Abstract:
OBJECTIVE:To identify and treat the gamete responsible for complete fertilization failure with intracytoplasmic sperm injection (ICSI) using a newly proposed assisted gamete treatment (AGT). DESIGN:Prospective cohort study. SETTING:Center for reproductive medicine. PATIENT(S):One-hundred and fourteen couples with an adequate number of spermatozoa for ICSI and a fertilization rate of ≤10%, after controlling for maternal age. INTERVENTION(S):Couples with an oocyte-related oocyte activation deficiency (OAD) underwent a subsequent cycle with a modified superovulation protocol; couples with sperm-related OAD had an additional genetic and epigenetic assessment to identify mutations and expression levels of the corresponding genes. MAIN OUTCOME MEASURE(S):Treatment cycle outcome for couples undergoing ICSI with either a modified superovulation protocol or AGT compared with their historical cycle. RESULT(S):A total of 114 couples matched the inclusion criteria, representing approximately 1.3% of the total ICSI cycles performed at our center, with age-matched controls. Fifty-two couples were confirmed negative for sperm-related OAD by the phospholipase Cζ (PLCζ) assay, indicating oocyte-related factors in their failed fertilization cycles. Couples were treated by one of two AGT protocols, AGT-initial or AGT-revised, in a subsequent attempt that was compared with their historical cycle. Subsequent ICSI cycles with a tailored superovulation protocol yielded significantly higher fertilization (59.0% vs. 2.1%) and clinical pregnancy (28.6% vs. 0) rates. In 24 couples (mean ± standard deviation: maternal age, 35.6 ± 5 years; paternal age, 39.8 ± 6 years) sperm-related OAD was confirmed; in four men, a deletion on the PLCZ1 gene was identified. Additional mutations were also identified of genes supporting spermiogenesis and embryo development (PIWIL1, BSX, NLRP5) and gene deletions confirming a complete absence of the subacrosomal perinuclear theca (PICK1, SPATA16, DPY19L). Subsequent AGT treatment provided higher fertilization (42.1%) and clinical pregnancy (36% vs. 0%) rates for couples with a history of impaired (9.1%) fertilization. A comparison of the two AGT protocols, AGT-initial or AGT-revised, revealed that the latter yielded even more favorable fertilization (37.6% vs. 45.9%) and clinical pregnancy (21.1% vs. 83.3%) rates. CONCLUSION(S):In couples with an oocyte-related OAD, tailoring the superovulation protocol resulted in successful fertilization, term pregnancies, and deliveries. In couples with a sperm-related OAD as determined by PLCζ assay, mouse oocyte activation test, and the assessment of gene mutations and function, AGT was successful. The AGT-revised protocol yielded an even higher fertilization rate than the AGT-initial protocol, resulting in the birth of healthy offspring in all couples who achieved a clinical pregnancy.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Cheung S,Xie P,Parrella A,Keating D,Rosenwaks Z,Palermo GDdoi
10.1016/j.fertnstert.2020.04.044subject
Has Abstractpub_date
2020-09-01 00:00:00pages
535-544issue
3eissn
0015-0282issn
1556-5653pii
S0015-0282(20)30408-8journal_volume
114pub_type
杂志文章abstract:OBJECTIVE:To evaluate surgical sperm retrieval rates in adolescents with Klinefelter syndrome and testosterone replacement therapy (TRT). DESIGN:Case series. SETTING:Academic medical center. PATIENT(S):Ten patients with Klinefelter syndrome, aged 14-22 years, treated with testosterone replacement and aromatase inhib...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.06.010
更新日期:2013-10-01 00:00:00
abstract:OBJECTIVE:To compare the FSH threshold level for follicle growth up to the preovulatory stage between superovulated and natural cycles. DESIGN:Normally cycling women were investigated during an untreated spontaneous cycle (control) and three cycles treated with FSH. SETTING:Infertility clinic in a university hospital...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)57610-9
更新日期:1995-06-01 00:00:00
abstract::The aim of this study was to ascertain whether an interrelationship exists between gonadal steroids and the inhibition of gonadotropin secretion by dopamine. The effect of dopamine infusion (4 micrograms/kg/minute intravenously) on gonadotropin plasma levels in four castrated men (18 to 23 years of age) and in four ag...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48273-7
更新日期:1984-12-01 00:00:00
abstract:OBJECTIVE:To report a case of complete androgen insensitivity syndrome (CAIS) with Müllerian duct persistence. DESIGN:Case report. SETTING:Academic hospital. PATIENT(S):A case of CAIS at 20 weeks' gestational age, and three male and one female 20-week-old fetuses for comparison. INTERVENTION(S):DNA screening for an...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.09.028
更新日期:2011-03-01 00:00:00
abstract::Obesity seems to exert a small but important extraovarian effect that may influence cycle outcome during assisted reproduction. Future investigation should focus on elucidating whether the endometrium is truly responsible for this deficit, and if it is, determining the mechanisms involved. ...
journal_title:Fertility and sterility
pub_type: 评论,杂志文章
doi:10.1016/j.fertnstert.2007.03.079
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:To investigate the influence of folic acid supplementation on the follicular fluid concentrations of folate and total homocysteine and their relationship to the diameter of the follicle. DESIGN:Observational study. SETTING:Tertiary referral fertility clinic at the Erasmus MC, University Medical Center, Rott...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.06.036
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVE:To evaluate the impact of female smoking on early embryo development in vitro using a time-lapse system. DESIGN:Retrospective analysis of prospectively collected database. SETTING:Assisted reproductive technology (ART) program. PATIENT(S):A total of 135 infertile couples undergoing IVF-intracytoplasmic spe...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.01.136
更新日期:2013-06-01 00:00:00
abstract:OBJECTIVE:To determine the feasibility of performing in vivo magnetic resonance elastography (MRE) for uterine leiomyoma. DESIGN:Pilot study. SETTING:Academic medical center. PATIENT(S):Six subjects planning surgical excision of uterine leiomyomas. INTERVENTION(S):MRE before planned surgery. MAIN OUTCOME MEASURE(S...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/j.fertnstert.2010.06.004
更新日期:2011-01-01 00:00:00
abstract::The interaction of medroxyprogesterone acetate (MPA) with cortisol binding to corticosteroid-binding globulin (CBG) was studied with the use of an aqueous two-phase system with polyethylene glycol and dextran for equilibrium partition. Competitive binding analyses were also performed for progesterone (P), levonorgestr...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48612-7
更新日期:1985-06-01 00:00:00
abstract::In 1951 it was shown that human spermatozoa contained cytochromes a, b, and c. It was noted that the cytochromes were confined to the sperm and were not found in seminal fluid. More recently we have observed in seminal plasma a soluble component that has a typical spectrum of a reduced cytochrome b, but can be disting...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1981-03-01 00:00:00
abstract:OBJECTIVE:To compare blastocyst production, after IVF and ICSI, from sheep oocytes of various quality. Sham-injected oocytes and parthenogenetic activated oocyte groups were considered as control. DESIGN:Prospective experimental study. SETTING:University. ANIMAL(S):Three- to 6-month-old sheep. INTERVENTION(S):Oocyt...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.12.043
更新日期:2012-04-01 00:00:00
abstract::This prospective study assessed von Willebrand factor (VWF) expression in the endometrium using immunohistochemical staining in women with menorrhagia with or without von Willebrand disease (VWD) compared with a control group of women with normal menstrual loss. Endometrial VWF expression is significantly lower in wom...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.02.055
更新日期:2010-11-01 00:00:00
abstract::We found the prevalence of ovarian cancer in infertile women less than 40 years at the time of planned microsurgery for infertility to be 1 in 95. This may represent a greater prevalence of ovarian cancer than that of all women less than 40 years. Importantly, laparoscopy did not find the ovarian cancer in these women...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
abstract:OBJECTIVE:To review current knowledge regarding recombinant DNA technology and its safety and efficacy in relation to recombinant gonadotropin production. DATA IDENTIFICATION AND SELECTION:Studies that relate specifically to recombinant DNA technology, method of laboratory production, and the clinical aspects of using...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/s0015-0282(16)58437-4
更新日期:1996-08-01 00:00:00
abstract::A prospective randomized study of 243 embryo transfers revealed that the use of blastocysts in assisted reproductive technology is not more effective than the use of day 2 and day 3 embryos. ...
journal_title:Fertility and sterility
pub_type: 临床试验,信件,随机对照试验
doi:10.1016/j.fertnstert.2003.07.016
更新日期:2004-02-01 00:00:00
abstract::The cumulative conception rate of 31 infertile women undergoing a second tuboplasty using microsurgery was compared with that 71 women undergoing a single microsurgical tuboplasty. The follow-up period ranged from ranged from 1 to 61/2 years. Five (16%) of the patients in the repeat tuboplasty group conceived; there w...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)45979-0
更新日期:1982-01-01 00:00:00
abstract:OBJECTIVE:To compare danazol and gestrinone treatment as preoperative endometrial preparation for operative hysteroscopy. DESIGN:Prospective, randomized clinical study. SETTING:University department of gynecological, obstetrical sciences and reproductive medicine. PATIENT(S):One hundred thirty-five patients with end...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2005.09.034
更新日期:2006-04-01 00:00:00
abstract:OBJECTIVE:To determine the expression profile of small noncoding RNAs (sncRNAs) in leiomyoma, which has not been investigated to date. DESIGN:Laboratory-based investigation. SETTING:Academic center. PATIENT(S):Women undergoing hysterectomy for benign indications. INTERVENTION(S):Next-generation sequencing and scree...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2018.01.034
更新日期:2018-05-01 00:00:00
abstract:OBJECTIVE:To investigate the impact of prolonged ovarian stimulation on pregnancy outcomes in IVF cycles with fresh day 3 ET. DESIGN:Retrospective cohort study. SETTING:University-affiliated center. PATIENT(S):All patients initiating their first IVF cycle with fresh day 3 ET. Prolonged ovarian stimulation was define...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.09.044
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:The purpose of the study was to describe and to compare the rate of rise of human chorionic gonadotropin (hCG) in vanishing twin and normally progressing twin pregnancies during the first trimester. DESIGN:All patients with twin pregnancies between 1985 and 1989 were prospectively studied. Human chorionic go...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)54475-6
更新日期:1991-08-01 00:00:00
abstract:OBJECTIVE:To assess the level of lipid peroxidation (LP) and vitamin E in the follicular fluid and serum of infertile patients, with or without endometriosis, who were submitted to ovulation induction for assisted reproduction procedures. DESIGN:Prospective study. SETTING:Assisted conception unit, university hospital...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/j.fertnstert.2007.10.072
更新日期:2008-12-01 00:00:00
abstract:OBJECTIVE:To determine whether fallopian sperm perfusion significantly improves pregnancy rates over intrauterine insemination (IUI) in infertile patients. DESIGN:Prospective, randomized, controlled trial and metaanalysis of the literature. SETTING:University-based infertility practice. PATIENT(S):Patients undergoin...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,meta分析,随机对照试验
doi:10.1016/s0015-0282(99)00068-0
更新日期:1999-05-01 00:00:00
abstract:OBJECTIVE:To examine the spatiotemporal expression of CD9 protein in the peri-implantation mouse uterus as well as the effects of ovarian steroid hormones on CD9. DESIGN:Experimental animal study. SETTING:Reproductive biological center of Chinese Academy of Sciences. ANIMAL(S):Female Kunming white strain mice (6-8 w...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.07.1525
更新日期:2007-03-01 00:00:00
abstract:OBJECTIVE:To compare the effects of intrauterine and subdermal administration of levonorgestrel on control of bleeding and on the endometrium in postmenopausal hormone replacement therapy. INTERVENTIONS:Nineteen women started continuous oral E2 valerate therapy (2 mg daily) together with continuous parenteral progesti...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(16)57365-8
更新日期:1995-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the effectiveness of sublingual misoprostol for cervical ripening before diagnostic hysteroscopy in premenopausal women. DESIGN:Placebo-controlled, double-blind, randomized trial. SETTING:University hospital. PATIENT(S):Fifty-two women with an indication for diagnostic hysteroscopy. INTERVENTI...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2009.01.073
更新日期:2010-05-01 00:00:00
abstract::The beta-adrenergic agonist isoproterenol was infused intravenously for 4 hours during the midluteal phase of the cycle in six normal women. A rapid increase in cardiac output occurred for the entire duration of the isoproterenol infusion and serum estrogen and progesterone levels (but not luteinizing hormone levels) ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)47712-5
更新日期:1984-03-01 00:00:00
abstract::Serum prolactin was studied in 25 fertile and 127 infertile men. The latter included 91 oligospermic, 27 azoospermic, and 9 hypogonadotropic hypogonadal men. The mean prolactin level in all three groups of infertile men was significantly above that of the fertile group. There was no correlation among serum levels of f...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)42259-4
更新日期:1976-12-01 00:00:00
abstract::The effectiveness of ovulation induction with clomiphene citrate or human menopausal gonadotropins was evaluated in 52 infertile women with stage I or stage II endometriosis and ovulatory dysfunction: anovulation or luteinized unruptured follicle (LUF) syndrome before (group I) and after (group II) danazol treatment. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)49811-0
更新日期:1986-11-01 00:00:00
abstract::Cooling experiments of abdominal testes in adult, naturally cryptorchid pigs indicate that spermatogenic arrest in abdominal testes is not due to an inborn defect, but is caused solely by maintenance of the testis at abdominal temperature. It is postulated that failure of spermatogenic cells to differentiate after orc...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)44364-5
更新日期:1979-11-01 00:00:00
abstract:OBJECTIVE:To present an alternative technique for the creation of a neovagina. DESIGN:Case study. SETTING:Academic university gynecology clinic. PATIENT(S):A patient diagnosed with müllerian agenesis. INTERVENTION(S):Laparoscopically assisted creation of a neovagina by using peritoneum. MAIN OUTCOME MEASURE(S):Pat...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(00)00700-7
更新日期:2000-09-01 00:00:00