Presence of soluble b-type cytochrome in human seminal fluid.

Abstract:

:In 1951 it was shown that human spermatozoa contained cytochromes a, b, and c. It was noted that the cytochromes were confined to the sperm and were not found in seminal fluid. More recently we have observed in seminal plasma a soluble component that has a typical spectrum of a reduced cytochrome b, but can be distinguished from the b-type cytochromes in other mammalian tissue. This substance, seminal plasma cytochrome (SPC) has not been described before. The spectrum of reduced SPC has peaks of 4290 A and 5620 A. The reduction can be induced chemically with sodium dithionite or enzymatically using reduced nicotinamide adenine dinucleotide as electron donor. The reduced SPC can bind carbon monoxide, giving an absorption peak in the range of 4140 A to 4250 A. SPC was present in semen from vasectomized men and infertility patients with azoospermia, as well as in the seminal plasma of normal donors. This indicates that the enzyme is not present as an artifact caused by leakage from spermatozoa. The possible function of SPC in seminal plasma is unknown at the present time.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Trifunac NP,Bernstein GS

subject

Has Abstract

pub_date

1981-03-01 00:00:00

pages

337-41

issue

3

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)45383-5

journal_volume

35

pub_type

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