Abstract:
OBJECTIVE:To determine the expression profile of small noncoding RNAs (sncRNAs) in leiomyoma, which has not been investigated to date. DESIGN:Laboratory-based investigation. SETTING:Academic center. PATIENT(S):Women undergoing hysterectomy for benign indications. INTERVENTION(S):Next-generation sequencing and screening of an sncRNA database with confirmatory analysis by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). MAIN OUTCOME MEASURE(S):Expression profile of sncRNAs in leiomyoma and matched myometrium. RESULT(S):Screening our previously determined RNA sequencing data with the sncRNA database resulted in identification of 15 small nuclear (sn) RNAs, 284 small nucleolar (sno) RNAs, 98 Piwi-interacting (pi) RNAs, 152 transfer (t) RNAs, and 45 ribosomal (r) RNAs, of which 15 snoRNAs, 24 piRNAs, 7 tRNAs, and 6 rRNAs were differentially expressed at a 1.5-fold change cutoff in leiomyoma compared with myometrium. We selected 5 snoRNAs, 4 piRNAs, 1 tRNA, and 1 rRNA that were differentially expressed and confirmed their expression in paired tissues (n = 20) from both phases of the menstrual cycle with the use of qRT-PCR. The results indicated up-regulation of the snoRNAs (SNORD30, SNORD27, SNORA16A, SNORD46, and SNORD56) and down-regulation of the piRNAs (piR-1311, piR-16677, piR-20365, piR-4153), tRNA (TRG-GCC5-1), and rRNA (RNA5SP202) expression in leiomyoma compared with myometrium (P<.05). The pattern of expression of these sncRNAs was similar to RNA sequencing analysis, with no menstrual cycle-dependent differences detected except for SNORD30. Because Argonaute 2 (AGO2) is required for sncRNA-mediated gene silencing, we determined its expression and found greater abundance in leiomyoma. CONCLUSION(S):Our results provide the first evidence for the differential expression of additional classes of sncRNAs and AGO2 in leiomyoma, implicating their roles as a gene regulatory mechanism.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Chuang TD,Xie Y,Yan W,Khorram Odoi
10.1016/j.fertnstert.2018.01.034subject
Has Abstractpub_date
2018-05-01 00:00:00pages
919-929issue
5eissn
0015-0282issn
1556-5653pii
S0015-0282(18)30059-1journal_volume
109pub_type
杂志文章abstract::Selenium (Se) was measured in the semen of 125 men from couples consulting for infertility. A mean concentration of 71.3 +/- 29.7 ng/ml of semen was found, with a range of 7 to 230 ng/ml. More than 85% of the Se is in the seminal plasma. There was a significant positive correlation between sperm count and semen Se. Sp...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00
abstract::Meta-analyses have become an increasingly popular method of drawing conclusions when there are multiple publications addressing a particular topic. While the statistical calculations are straightforward, many of the decisions regarding the research question, populations of interest, and inclusion/exclusion criteria ar...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2011.05.029
更新日期:2011-07-01 00:00:00
abstract:OBJECTIVE:To provide a step-by-step description of our published technique of single-site robot-assisted laparoscopic myomectomy with the goal of promoting its safe adoption. DESIGN:Surgical video tutorial. SETTING:University medical center. PATIENT(S):Ten women undergoing single-site robot-assisted laparoscopic myo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2015.07.1159
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVE:To investigate whether a history of previous ectopic pregnancy is an independent risk factor for failure of methotrexate therapy in subsequent ectopic pregnancies and whether the previous treatment method influenced failure. SETTING:Inner-city teaching hospital. DESIGN:Retrospective case review. PATIENT(S)...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2003.09.070
更新日期:2004-05-01 00:00:00
abstract::The effects of various doses of indomethacin and ketoprofen as compared with placebo were examined in 100 oligospermic patients who participated in this study. It was found that the treatment increased sperm count, sperm motility, and fertilizing capacity. The radioimmunoassay examination showed an increase in plasma ...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(16)48081-7
更新日期:1984-09-01 00:00:00
abstract:OBJECTIVE:To determine whether mutations in the gene for FSH beta are present, and possibly etiologic, in some patients with 46,XX premature ovarian failure (POF). DESIGN:DNA samples obtained from 18 study patients with POF and two menopausal fertile controls were studied by Southern blot analysis. DNA sequencing was ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56286-4
更新日期:1993-11-01 00:00:00
abstract::In 80 normal puerperae, serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), including human chorionic gonadotropin (hCG/LH), and prolactin (PRL) levels were evaluated 6 to 29 hours after vaginal delivery. In these puerperae, PRL levels were higher and FSH levels were lower than in menstruating women; h...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)46103-0
更新日期:1982-03-01 00:00:00
abstract::Reportedly, gamete intrafallopian transfer and zygote intrafallopian transfer are successful methods in assisted conception. This pilot study describes the experiences and results of a recently developed technique of vaginal transcervical intrafallopian transfer. In a group of 38 women with unexplained infertility, oo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53704-2
更新日期:1990-08-01 00:00:00
abstract:OBJECTIVE:To compare the rate of ectopic pregnancy (EP) with fresh versus frozen blastocyst transfer in our program during the same time period. DESIGN:Retrospective analysis. SETTING:University IVF program. PATIENT(S):Women who achieved a clinical pregnancy after IVF between 1998 and 2005. INTERVENTION(S):In our p...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.12.005
更新日期:2007-09-01 00:00:00
abstract::In this study, both IVF-ET cycle and pregnancy outcome were compared with increases seen in P concentration during the 24-hour period from 12 hours before to 12 hours after hCG administration. A significantly higher PR (P < 0.001) was seen in women who had at least a threefold increase in P during this time period. Fu...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract::This study indicates that SP occur in a higher percentage than theoretically expected after IVE-ET treatment, but it is not conclusive as to whether the SP is independent or a result of the treatment or operative laparoscopy. Our results are in agreement with those of previous studies that have shown that SP occur wit...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)49289-7
更新日期:1986-04-01 00:00:00
abstract:OBJECTIVE:To assess the endocrine, paracrine, and autocrine milieu in patients with endometriosis on the basis of the measurement of several cytokines in serum and follicular fluid (FF) and in vitro culture of granulosa luteal cells. DESIGN:Case-control study. SETTING:In vitro fertilization program at the Instituto V...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00204-0
更新日期:1998-09-01 00:00:00
abstract:OBJECTIVE:To compare the incidence of noncompliance measured objectively by a home use fertility monitor with the traditional self-reported incidence of compliance in a study of a new method of contraception. DESIGN:Prospective cohort study. SETTING:A large family planning clinic in Edinburgh. PATIENT(S):Thirty-two ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(01)02899-0
更新日期:2001-12-01 00:00:00
abstract:OBJECTIVE:To determine whether there is a difference in fibrinolytic compounds in endometriotic tissue, endometrium, peritoneal fluid (PF), and plasma from women with endometriosis and in endometrium and PF from healthy women. DESIGN:Prospective study. SETTING:Two university clinics. PATIENT(S):Regularly menstruatin...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00285-4
更新日期:1998-11-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of obesity on the outcome of testicular sperm extraction (TESE) and assisted reproductive technology. DESIGN:Clinical retrospective study. SETTING:Center for reproductive medicine at a tertiary-care university hospital. PATIENT(S):Nine hundred seventy patients with nonobstructive azo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.10.025
更新日期:2013-02-01 00:00:00
abstract:OBJECTIVE:To reassess the predictive value of detecting sperm cells in men with AZFb or AZFb-c deletions. DESIGN:Retrospective analysis of previously reported men with AZFb or AZFb-c deletions and the addition of six new cases. SETTING:Fertility institution. PATIENT(S):Men with both sequence tagged site marker ident...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2011.01.162
更新日期:2011-05-01 00:00:00
abstract::Seven homozygotes for F-XIII deficiency were found in a large inbred Israeli-Arab kindred. One affected woman had early abortions. Two affected men and one probably affected man had children, two of whom also were affected. Paternity was confirmed by segregation of HLAs and erythrocyte antigens and enzymes. This is th...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)49351-9
更新日期:1986-05-01 00:00:00
abstract::Baboons were immunized using a synthetic peptide adjuvant with two purified pig zona pellucida glycoproteins. The major zona pellucida glycoprotein (ZPI) was purified by preparative isoelectric focusing, and the 80 K deglycosylated zona pellucida protein (ZPIII) was purified by preparative sodium dodecyl sulfate polya...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60861-0
更新日期:1989-08-01 00:00:00
abstract::Certainly, varicoceles can have a substantive effect on sperm production in infertile males. In addition, varicocele repair may optimize sperm production. However, additional studies and evaluation of the role of varicocele repair in men with this condition (nonobstructive azoospermia) are warranted. ...
journal_title:Fertility and sterility
pub_type: 评论,杂志文章
doi:10.1016/j.fertnstert.2010.11.057
更新日期:2011-02-01 00:00:00
abstract::We tested the hypothesis that prenatal exposure to cigarette smoke is associated with impaired fertility in adulthood. Fertility for nearly 600 couples was quantified by counting the number of noncontraception cycles required to conceive. Exposure in utero was assessed by asking couples about their parents' cigarette ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:
更新日期:1986-09-01 00:00:00
abstract:OBJECTIVE:To evaluate the effects of tumor necrosis factor-alpha (TNF-alpha) on porcine oocyte maturation, spindle dynamics, and chromosome alignment. DESIGN:Controlled, prospective study. SETTING:University hospital and IVF research laboratory. ANIMAL(S):Ovaries collected from slaughtered prepubertal gilts. MAIN O...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2009.01.131
更新日期:2010-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the efficiency of using a continuous (one-step) protocol with a single medium for the culture of human embryos in a time-lapse incubator (TLI). DESIGN:Prospective cohort study on sibling donor oocytes. SETTING:University-affiliated in vitro fertilization (IVF) center. PATIENT(S):Embryos from 59...
journal_title:Fertility and sterility
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.fertnstert.2015.11.038
更新日期:2016-03-01 00:00:00
abstract:OBJECTIVE:To assess vaginal development, sexual activity, and the efficacy of vaginal dilator therapy in women with vaginal agenesis (Mayer-Rokitansky-Kuster-Hauser syndrome). DESIGN:Retrospective case review. SETTING:Tertiary referral university teaching hospital clinic for disorders of sexual development and differ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.05.060
更新日期:2011-08-01 00:00:00
abstract::Where histology used the presence of glands and/or stroma in the myometrium as pathognomonic for adenomyosis, imaging uses the appearance of the myometrium, the presence of striations, related to the presence of endometrial tissue within the myometrium, the presence of intramyometrial cystic structures and the size an...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2018.01.006
更新日期:2018-03-01 00:00:00
abstract:OBJECTIVE:To determine the expression of glutathione peroxidase (GPx) in the eutopic and ectopic endometria during the menstrual cycle in endometriosis and adenomyosis. DESIGN:Immunohistochemical identification of GPx in endometrial tissues identified using the polyclonal antibody. SETTING:Department of obstetrics an...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(00)00638-5
更新日期:2000-08-01 00:00:00
abstract::Twenty-seven patients underwent ultrasonographic and clinical monitoring of a spontaneous cycle so that human chorionic gonadotropin administration could be timed in an attempt to schedule the screening laparoscopy at the optimal time for oocyte aspiration. Inadequate ovarian access was encountered in only 2 of 26 lap...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)46871-8
更新日期:1983-03-01 00:00:00
abstract:OBJECTIVE:To explore the role of tumor necrosis factor (TNF) α, an early embryonic product, on endometrial epithelial cell migration and endometrial milk fat globule-epidermal growth factor 8 protein (MFG-E8) production. DESIGN:In vitro study. SETTING:Academic center. INTERVENTION(S):Ishikawa cells, used as surrogat...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2013.10.032
更新日期:2014-02-01 00:00:00
abstract:OBJECTIVE:To determine if fertility treatment is associated with increased risk of severe maternal morbidity (SMM) compared with spontaneous pregnancies. DESIGN:Retrospective cohort study. SETTING:Academic medical center. PATIENT(S):In 2012, 6,543 women delivered live births >20 weeks gestation at our center. Women ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2016.03.039
更新日期:2016-08-01 00:00:00
abstract::To study the prevalence of osteoporosis and hyperandrogenism in neuroleptic-induced hyperprolactinemia, the authors evaluated 10 patients. Three were amenorrheic, while seven had oligomenorrhea. Nine patients had galactorrhea. The Ferriman-Gallway hirsutism score was 12 +/- 2. Vaginal smear maturation value was 53 +/-...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60365-5
更新日期:1988-12-01 00:00:00
abstract:OBJECTIVE:To study the distribution of a set of polymorphic alleles of genes encoding key regulators of the menstrual cycle in cohorts of women with different levels of fertility. DESIGN:Prospective cohort study. SETTING:University hospital. PATIENT(S):Two hundred fertile women, who reported to have conceived within...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.05.048
更新日期:2012-10-01 00:00:00