Abstract：
OBJECTIVE:To characterize and to purify the growth substances for mouse embryo fibroblasts (NIH/3T3) and endometrial-like cells of the peritoneal fluid of women with endometriosis. DESIGN:A pool of 102 mL of peritoneal fluid (PF) collected from 24 women with laparoscopic evidence of endometriosis was dialyzed and thereafter chromatographed onto carboxymethyl (CM)-sepharose, heparin-sepharose, cartridges of C18 silica, and reverse-phase high-performance liquid chromatography (reverse-phase HPLC). Mitogenic activity throughout various steps of chromatography was monitored by the increase of the (3H)-thymidine incorporation into NIH/3T3 fibroblasts and enzymatically isolated epithelial cells of rabbit endometrium. RESULTS:Peritoneal fluid of women with endometriosis contained mitogen(s) for NIH/3T3 fibroblasts that were retained on and eluted from CM-sepharose, heparin-sepharose, cartridges of C18 silica, and reverse-phase HPLC columns performed sequentially. Mitogenic material purified 2,500-fold exerted bioactivity on NIH/3T3 and the enzymatically isolated epithelial cells from rabbit endometrium. CONCLUSION:Peritoneal fluid of women with endometriosis contains heparin-binding growth factors that can be purified by sequential chromatography on CM-sepharose, heparin-sepharose, and reverse-phase HPLC columns. The presence in the PF of potent mitogens for fibroblasts and endometrium-derived epithelial cells could play an important role in the pathogenesis of endometriosis.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Koutsilieris M,Niklinski W,Frenette G,Lemay Asubject
Has Abstractpub_date
1993-01-01 00:00:00pages
93-7issue
1eissn
0015-0282issn
1556-5653pii
S0015-0282(16)55621-0journal_volume
59pub_type
杂志文章abstract：:Carbohydrate and lipid metabolism was evaluated in 31 women before and after 1 year's use of a progesterone-releasing intrauterine device. The studies involved measuring blood glucose and plasma insulin levels during a 3-hour oral glucose tolerance test and also measuring fasting cholesterol and triglyceride levels. T...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43932-4
更新日期：1979-04-01 00:00:00
abstract：OBJECTIVE:To evaluate whether the efficacy of naltrexone administration in patients with hypothalamic amenorrhea correlates to the response to an acute naloxone test. DESIGN:Thirty patients with hypothalamic amenorrhea associated with weight loss were studied. After naloxone test (4 mg in bolus IV) patients were divid...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)57908-4
更新日期：1995-11-01 00:00:00
abstract：OBJECTIVE:To develop and validate a specific simple measure of insulin sensitivity using oral glucose tolerance test (OGTT) values for lean polycystic ovary syndrome (PCOS) women. DESIGN:Retrospective study. SETTING:Gynecologic Outpatient Clinic of University Hospital, affiliated with Unit of Gynecologic Endocrinolog...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.04.004
更新日期：2014-07-01 00:00:00
abstract：:Asherman's syndrome is described in today's context. Meaningful literature as it relates to the description and management of this entity is reviewed, and the forms of treatment used at the Hospital of the University of Pennsylvania or at the Philadelphia General Hospital are presented. Asherman's syndrome describes...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)39918-6
更新日期：1973-09-01 00:00:00
abstract：OBJECTIVE:To examine the expression of the heat shock protein hsp70-2, and the possible relationship with the pathogenesis of male infertility. DESIGN:Prospective study. SETTING:Reproductive testing laboratory in a university hospital. PATIENT(S):Men undergoing testicular biopsy during an investigation of subfertili...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)02892-8
更新日期：2001-12-01 00:00:00
abstract：:Nitrofurantoin sodium and nitorfurazone, in certain critical concentrations, have the ability to immobilize spermatozoa. In this study, this effect was consisitent with both 5:1, and 10:1 dilutions. Histologic examination of the effects of these drugs as intraoperative vas irrigants revealed no apparent tissue toxicit...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)41168-4
更新日期：1975-06-01 00:00:00
abstract：OBJECTIVE:To evaluate the reproductive outcome of infertile couples undergoing assisted reproduction techniques (ART) with or without human papillomavirus (HPV) semen infection. DESIGN:Cross-sectional clinical study. SETTING:Units of andrology, reproductive medicine, and gynecology. PATIENT(S):A total of 226 inferti...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.09.018
更新日期：2016-01-01 00:00:00
abstract：:There is a paucity of data concerning the factors involved in the growth of uterine leiomyomata. The expression of mRNAs encoding EGF and the EGF receptor in myometrial and leiomyoma cultures suggest that EGF may be involved in the autocrine/paracrine regulation of human uterine leiomyomata and myometrial growth. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54681-0
更新日期：1991-11-01 00:00:00
abstract：OBJECTIVE:To determine whether cognitive behavior therapy (CBT) targeted to problematic attitudes common among women with functional hypothalamic amenorrhea would restore ovarian function. DESIGN:Randomized, prospective, controlled intervention. SETTING:Clinical research center in an academic medical institution. PA...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(03)01124-5
更新日期：2003-10-01 00:00:00
abstract：:Since the late 1980s premature progesterone elevation has repeatedly caught the attention of investigators, not only for its importance, but also because studies have shown differing results and conclusions, constituting an unexplainable sequence of doubts and uncertainties. This issue's Views and Reviews section seek...
journal_title：Fertility and sterility
pub_type：
doi：10.1016/j.fertnstert.2018.02.134
更新日期：2018-04-01 00:00:00
abstract：OBJECTIVE:To determine whether there is significant quality of life score improvement after uterine artery embolization (UAE) and to compare UAE and myomectomy outcomes. DESIGN:Prospective cohort controlled study. SETTING:Sixteen medical centers in the United States. PATIENT(S):One hundred forty-nine UAE patients an...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究
doi：10.1016/j.fertnstert.2005.05.074
更新日期：2006-01-01 00:00:00
abstract：:Cryopreservation has become a central pillar in assisted reproduction, reflected in the exponential increase of "freeze all" cycles in the past few years. Vitrification makes it possible to cool and warm human eggs and embryos with far less cryo-damage than 'slow-freeze' and allows nearly intact survival of embryos wi...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2019.12.009
更新日期：2020-02-01 00:00:00
abstract：:To further define the role of CO2 laser in infertility surgery, the authors compared the immediate tissue damage and the subsequent healing patterns that followed the randomized use of CO2 laser and electrocautery on opposite sides of the reproductive tracts of 20 mature, female rabbits. Blind histopathologic evaluati...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)59603-4
更新日期：1987-12-01 00:00:00
abstract：:Three groups of women with different types of ovulatory dysfunction who had failed to conceive on conventional therapy were treated with pulsatile gonadotropin-releasing hormone (GnRH). Group A consisted of nine patients with luteal phase defect; group B included four patients with apparently normal menstrual cycles b...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48971-5
更新日期：1985-11-01 00:00:00
abstract：OBJECTIVE:To investigate whether ghrelin signaling is involved in the pathogenesis of male factor infertility induced by leptin deficiency. DESIGN:Experimental study. SETTING:University academic medical center. ANIMAL(S):Ten-week-old C57BL/6J mice and ob/ob mice. INTERVENTION(S):Western blotting, (quantitative) rev...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.11.022
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVE:To evaluate whether ovarian function might have an influence on the adrenal hyperandrogenism present in patients with functional ovarian hyperandrogenism. DESIGN:Controlled clinical study. SETTING:Tertiary institutional hospital. PATIENT(S):Twenty-nine hirsute women with functional ovarian hyperandrogenism...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)81362-3
更新日期：1997-04-01 00:00:00
abstract：OBJECTIVE:To summarize current understanding of the effects of novel and prior coronaviruses on human reproduction, specifically male and female gametes, and in pregnancy. DESIGN:Review of English publications in PubMed and Embase to April 6, 2020. METHOD(S):Articles were screened for reports including coronavirus, r...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.04.025
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVE:To examine the frequency of anomalies of the vas deferens and the frequency of mutations of the cystic fibrosis transmembrane regulator (CFTR) gene in male candidates for intracytoplasmic sperm injection (ICSI) who had severe oligoasthenoteratozoospermia. DESIGN:The clinical data for male candidates for ICSI...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00050-8
更新日期：1998-05-01 00:00:00
abstract：:D-group chromosome translocations have been associated in isolated cases with infertility in males. In this study, we demonstrated the effect of a translocated D-group chromosome on spermatogenesis as it had segregated among three male members of one family. Although the father, who carried the translocation, was obvi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)41651-1
更新日期：1976-02-01 00:00:00
abstract：OBJECTIVES:To test the hypothesis that fertility is reduced in baboons with endometriosis. DESIGN:Prospective controlled follow-up study. SETTING:Institute of Primate Research, Nairobi Kenya. SUBJECTS:Seventy-one baboons, including 34 controls (normal pelvis) and 37 animals with histologically proven endometriosis (...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1996-11-01 00:00:00
abstract：:This immunohistochemical study shows that platelet endothelial cell adhesion molecule-1 (PECAM-1), known to play a role in neoangiogenesis and vascular development, is strongly and homogeneously expressed in endothelial cells lining blood vessels from red and black pelvic endometriotic lesions. The distribution of PEC...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.05.075
更新日期：2004-10-01 00:00:00
abstract：:Previous investigations have demonstrated the propensity of strong IgA-class sperm autoantibodies to impede fertilization. However, because there has not been a general consensus on this issue, the aim of this retrospective analysis was to focus on the effects of different levels of IgA-class antibodies on each stage ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.02.089
更新日期：2006-09-01 00:00:00
abstract：OBJECTIVE:To assess whether provision of an educational DVD was more effective in increasing the uptake of elective single embryo transfers (eSET) than an educational brochure in an IVF population. DESIGN:Randomized controlled trial. SETTING:Private IVF clinic. PATIENT(S):One hundred thirty-one couples starting thei...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2009.03.080
更新日期：2010-07-01 00:00:00
abstract：OBJECTIVE:To assess the use and effectiveness of IVF in a cohort of women undergoing surgery for endometriosis. DESIGN:Cohort study. SETTING:University hospital. PATIENT(S):Four hundred thirty-eight patients who attempted to become pregnant after conservative surgery for endometriosis. INTERVENTION(S):Interview. M...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.02.004
更新日期：2009-05-01 00:00:00
abstract：:To date, ovum donation (OD) has involved luteinizing hormone (LH) synchronization between recipient and donor for normally cycling women, and a complex steroid replacement regimen given on a sequential and incremental basis for women with primary or secondary ovarian failure. The authors designed a simple hormonal reg...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)59354-6
更新日期：1987-08-01 00:00:00
abstract：:In women with polycystic ovary syndrome, metformin administration, not laparoscopic ovarian drilling, reduces plasminogen activator inhibitor 1 (PAI-1) activity. The lack of a decrease in PAI-1 activity is related to a high risk for miscarriage. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.04.021
更新日期：2005-09-01 00:00:00
abstract：:To describe trends in the types of fertility-related medical services that women and/or their partners have received while trying to become pregnant, the author analyzed data from women aged 20-44 years who participated in the 2002 National Survey of Family Growth. Advice (74%) and infertility testing (59%) were the m...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.10.034
更新日期：2008-10-01 00:00:00
abstract：:In this prospective, randomized clinical trial, we tested the hypothesis that a needle-free CO(2) injection system (Biojector 2000) would be better tolerated by patients when compared with traditional sharp needles used for gonadotropin injections during stimulation for IVF. As measured by our questionnaire, the needl...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2009.03.013
更新日期：2009-10-01 00:00:00
abstract：OBJECTIVE:To assess the potential association between polycystic ovary syndrome (PCOS) and chromosomally aberrant miscarriage during treatment with assisted reproductive technology (ART). DESIGN:A retrospective, single-center study. SETTING:University-affiliated reproductive center. PATIENT(S):A total of 328 patient...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2019.01.026
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVE:To compare and quantify leukocyte populations at the tubal implantation site, in the tubal mucosa away from the implantation site and in the intrauterine decidua in women who have had an ectopic tubal pregnancy with leukocyte populations in the decidualized endometrium in women who have had a normal intrauter...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00005-3
更新日期：1998-04-01 00:00:00
