当前位置： SCI文献检索 > FERTILITY AND STERILITY期刊下所有文献 > Impact of follicular-fluid meiosis-activating sterol in an albumin-based formulation on the incidence of human pre-embryos with chromosome abnormalities.

Impact of follicular-fluid meiosis-activating sterol in an albumin-based formulation on the incidence of human pre-embryos with chromosome abnormalities.

Abstract：

OBJECTIVE:To evaluate the effect of adding follicular-fluid meiosis-activating sterol (FF-MAS) in a novel 0.2% recombinant human albumin-based formulation to cumulus-enclosed oocytes on chromosomal status and development of pre-embryos. DESIGN:Multicenter, prospective, randomized, open (double-blind for vehicle and FF-MAS groups), four parallel groups, controlled trial. SETTING:Four public IVF clinics in Denmark. PATIENT(S):Two hundred eighteen women undergoing IVF donated 483 oocytes. INTERVENTION(S):Follicle-stimulating hormone/hCG-primed cumulus-enclosed oocytes randomized to 4 hours of exposure to medium with 1 or 10 micromol/L of FF-MAS dissolved in 0.2% recombinant human albumin, medium with 0.2% recombinant human albumin (vehicle control), or medium alone (control) before insemination. MAIN OUTCOME MEASURE(S):Primary endpoint: incidence of human pre-embryos with chromosomal abnormalities. Secondary endpoint: fertilization rate, cleavage rate, and pre-embryo quality assessed after 68 hours of culture. RESULT(S):At pre-embryo level, the overall abnormality rates in the control, vehicle control, and 1- and 10-micromol/L FF-MAS groups were 53%, 39%, 42%, 53%, respectively, and at blastomere level 49%, 44%, 44%, and 48%, respectively. After 20 and 26 hours, the fertilization rates were between 67% and 71% in all groups. No differences in the cleavage rates were observed. CONCLUSION(S):The concentrations of FF-MAS in a novel 0.2% recombinant human albumin-based formulation of FF-MAS did not increase the risk of chromosomal abnormalities in pre-embryos or blastomeres. No statistically significant differences in fertilization rate, cleavage rate, or number of good quality pre-embryos were found among the four groups.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Loft A,Ziebe S,Erb K,Rasmussen PE,Agerholm I,Hauge B,Bungum M,Bungum L,Grøndahl C,Lyby K

doi

10.1016/j.fertnstert.2005.05.017

keywords:

subject

Has Abstract

pub_date

2005-10-01 00:00:00

pages

1269-76

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(05)01439-1

journal_volume

84 Suppl 2

pub_type

杂志文章,多中心研究,随机对照试验

在线工具

Impact of follicular-fluid meiosis-activating sterol in an albumin-based formulation on the incidence of human pre-embryos with chromosome abnormalities.