Abstract：
OBJECTIVE:The objective of the present studies was to determine whether luteinizing hormone (LH) depletes ascorbic acid in the preovulatory follicle. DESIGN:Controlled, prospective experimental study. SETTING:University-based research center. ANIMAL(S):Sprague-Dawley female rats. INTERVENTION(S):Follicular growth and ovulation were induced in immature rats by gonadotropin treatment. MAIN OUTCOME MEASURE(S):Analysis of ovary, follicle, and oocyte levels of ascorbic acid by colorimetric analysis and high-pressure liquid chromatography. RESULT(S):Ovarian ascorbic acid was maximally depleted (50%) within 2 h of LH treatment and was sustained for 8 h. Follicle ascorbic acid levels were unchanged 1 h after LH injection but were significantly reduced within 2 h (40%). Incubation of isolated preovulatory follicles for 3 h with hCG or with menadione (a generator of oxygen radicals) reduced ascorbic acid levels. Isolation of cumulus-enclosed or denuded oocytes depleted ascorbic acid to undetectable levels, but follicular ascorbic acid levels were only moderately depleted by isolation and incubation. Accumulation of ascorbic acid by oocytes was significantly enhanced by the presence of intact cumulus cells. CONCLUSION(S):Elevation of LH and the production of oxygen radicals deplete ascorbic acid in the preovulatory follicle.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Guarnaccia MM,Takami M,Jones EE,Preston SL,Behrman HRdoi
10.1016/s0015-0282(00)01550-8keywords:
subject
Has Abstractpub_date
2000-11-01 00:00:00pages
959-63issue
5eissn
0015-0282issn
1556-5653pii
S0015-0282(00)01550-8journal_volume
74pub_type
杂志文章abstract：OBJECTIVE:To investigate the expression profiles of microRNA (miRNA) in human testes showing different histopathological patterns. DESIGN:Microarray with quantitative real-time polymerase chain reaction (qRT-PCR) validation. SETTING:University research and clinical institutes. PATIENT(S):Azoospermic men who underwen...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.09.009
更新日期：2014-01-01 00:00:00
abstract：OBJECTIVE:To analyze the efficacy of intracytoplasmic sperm injection (ICSI) for "rescue" of failed conventional insemination cycles. DESIGN:Retrospective clinical study. SETTING:Private infertility clinic. PATIENT(S):Fifty-four couples with non-male factor infertility undergoing routine IVF within our clinic. INTE...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)00223-9
更新日期：1997-09-01 00:00:00
abstract：:Among 2,245 women, those who experienced a chemical pregnancy that failed to progress to a clinically recognized pregnancy or a spontaneous abortion on their first IVF cycle were more likely to discontinue IVF treatment than those whose first cycle ended before embryo transfer or who did not have a positive pregnancy ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.07.1732
更新日期：2009-04-01 00:00:00
abstract：OBJECTIVE:To evaluate the interactive effects of dietary soy and menstrual cycle phase on breast proliferation, apoptosis, and sex steroid receptor expression in premenopausal female cynomolgus monkeys. DESIGN:Animals were randomized to receive diets with either casein lactalbumin or soy as the protein source for 12 m...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.08.059
更新日期：2006-04-01 00:00:00
abstract：OBJECTIVE:To investigate the mental and general health of infertile women who had not sought medical advice for their recognized infertility and were therefore not represented in clinical populations. DESIGN:Longitudinal cohort study. SETTING:Population based. PATIENT(S):Participants in the Australian Longitudinal S...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.10.062
更新日期：2010-10-01 00:00:00
abstract：OBJECTIVE:To determine the effect of P on nitric oxide synthase (NOS) expression in human endometrial epithelial cells. DESIGN:Laboratory-based study. SETTING:University-based research institute. PATIENT(S):None. INTERVENTION(S):The effect of P on the expression of NOS protein isoforms was examined in an in vitro p...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.05.019
更新日期：2009-05-01 00:00:00
abstract：OBJECTIVE:To describe a woman with blepharophimosis-ptosis-epicanthus inversus syndrome and hypergonadotropic hypogonadism. DESIGN:Case report. SETTING:University medical center. PATIENT(S):One 25-year-old woman. INTERVENTION(S):Pedigree, hormone assays, and donor embryo transfer. MAIN OUTCOME MEASURE(S):Pregnancy...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.07.1763
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVE:To determine whether men with azoospermia are at an elevated risk of developing cancer in the years following an infertility evaluation. DESIGN:Cohort study. SETTING:United States andrology clinic. PATIENT(S):A total of 2,238 men with complete records were evaluated for infertility at a single andrology cl...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.05.022
更新日期：2013-09-01 00:00:00
abstract：OBJECTIVE:To determine whether serum interleukin (IL)-8 concentration can be measured in patients with ovarian endometrioma and whether this measurement is a useful tool in diagnosing this disease. DESIGN:A controlled clinical study and an in vitro study. SETTING:Department of Obstetrics and Gynecology, Tottori Unive...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.07.1355
更新日期：2008-10-01 00:00:00
abstract：:The promoter of P receptor isoform B (PR-B) has recently been shown to be hypermethylated in endometriotic tissues, but one question remains as what causes the aberrant methylation. We show that sustained stimulation of an epithelial-like endometriotic cell line with tumor necrosis factor alpha stimulation for 30 days...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.06.008
更新日期：2008-07-01 00:00:00
abstract：OBJECTIVE:To assess the association between recent cigarette smoking (CS) in female and male partners and assisted reproduction technology (ART) outcomes. DESIGN:Cohort prospective study. SETTING:University ART program in Chile. PATIENT(S):One hundred sixty-six couples seeking pregnancy through ART. INTERVENTION(S)...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.09.073
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the effects of tumor necrosis factor-alpha (TNF-alpha) on porcine oocyte maturation, spindle dynamics, and chromosome alignment. DESIGN:Controlled, prospective study. SETTING:University hospital and IVF research laboratory. ANIMAL(S):Ovaries collected from slaughtered prepubertal gilts. MAIN O...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.01.131
更新日期：2010-02-01 00:00:00
abstract：:Under guidance of an external pulsed magnetic field the Cuproferrogel iron oxide-copper-styrene maleic anhydride-dimethyl sulphoxide delivered into the rat/rabbit oviduct resulted in oocytes with granulated cytoplasm, zona enlargement, membrane disintegration, and finally loss of viability in 72 hours. Also, the perce...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.06.032
更新日期：2010-12-01 00:00:00
abstract：OBJECTIVE:To evaluate novel clinical markers of endometriosis including the neurotrophins brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), and neurotrophin 4/5 (NT4/5) and compare them to others previously reported in the literature including cancer antigen 125 (CA-125) and C-reactive protein (CRP)....
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.09.003
更新日期：2016-01-01 00:00:00
abstract：OBJECTIVE:To test the hypothesis that over a 4-week treatment period, Nal-Glu GnRH antagonist ([AcD2Nal1, D4ClPhe2, D3Pal3, Arg5, DGlu6 [AA], DAla10] GnRH) at a dose of 200 micrograms/kg per day SC would suppress levels of immunologically active and biologically active LH and FSH more completely than a dose of 100 micr...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1995-07-01 00:00:00
abstract：:Direct effects of a gonadotropin-releasing hormone agonist (GnRHa), danazol, or estrogen/progestogen (E/P) on experimental endometriosis were evaluated in castrated female rats. Endometrial explants decreased in size following castration, but there was no further change in the treatment groups. Histologic examination ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)59727-1
更新日期：1988-02-01 00:00:00
abstract：:The purpose of this study was to investigate the prognosis for fertility in children with hydrocele. Our results show that hydrocele seems to be more of a symptom than an actual pathological entity. It occurs both with an open and a closed processus vaginalis. Bilateral hydrocele seems to be a rare occurrence, mostly ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53467-0
更新日期：1990-04-01 00:00:00
abstract：OBJECTIVE:To compare the amount of regulated upon activation, normal T cell expressed and secreted (RANTES) and monocyte chemotactic protein 1 (MCP-1) in follicular fluid (FF) of patients with and without endometriosis and to determine their oocyte fertilization and pregnancy rates. DESIGN:Case-control study. SETTING...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.05.043
更新日期：2006-12-01 00:00:00
abstract：OBJECTIVE:To investigate whether polymorphisms at the promoter or 5'-untranslated region of annexin A5 gene (ANXA5) influence miscarriage. DESIGN:Case-control study and nested case-control study. SETTING:Hospitals. PATIENT(S):A total of 264 patients with two to nine recurrent pregnancy losses (RPLs) and 195 fertile ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.06.020
更新日期：2013-10-01 00:00:00
abstract：OBJECTIVE:To report the feasibility of ovarian cyst enucleation by using a laparoendoscopic single-site trocar through a transumbilical access. DESIGN:Case reports. SETTING:Teaching and research hospital. PATIENT(S):Three patients affected by large ovarian cysts. INTERVENTION(S):Enucleation of three large ovarian c...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.05.060
更新日期：2009-09-01 00:00:00
abstract：:Under pressure from involved families and time limitations, Israeli judges tend to authorize postmortem sperm retrieval and freezing, even in unmarried men and in contradiction to current, albeit non-legally binding, guidelines of the Attorney General. Postmortem sperm retrieval in our institution (2003 to June 2009) ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.10.035
更新日期：2010-08-01 00:00:00
abstract：OBJECTIVE:To validate recently proposed models of ovarian nongrowing follicle (NGF) decay associated with aging within the context of an independent data set. DESIGN:Prospective investigation. SETTING:Academic medical center. PATIENT(S):Normal appearing ovaries collected from 52 women (age 28-51 years) undergoing oo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.12.008
更新日期：2014-03-01 00:00:00
abstract：OBJECTIVE:To visualize by microhysteroscopy any possible lesions on the endocervix and endometrium made by the catheters commonly used for embryo transfer (ET). DESIGN:Prospective descriptive study. Tertiary fertility center (IFER). PATIENT(S):Twenty-three infertile patients underwent a mock transfer before a microhy...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(03)00607-1
更新日期：2003-08-01 00:00:00
abstract：:To further define the role of CO2 laser in infertility surgery, the authors compared the immediate tissue damage and the subsequent healing patterns that followed the randomized use of CO2 laser and electrocautery on opposite sides of the reproductive tracts of 20 mature, female rabbits. Blind histopathologic evaluati...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)59603-4
更新日期：1987-12-01 00:00:00
abstract：OBJECTIVE:To examine the expression and localization of Ets-1 in the rat ovary during the estrous cycle and pregnancy, and to investigate its effects on ovarian function. DESIGN:Prospective, randomized study. SETTING:Department of Physiology at Harbin Medical University. ANIMAL(S):Pubertal female Wistar rats. INTER...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.02.166
更新日期：2009-05-01 00:00:00
abstract：OBJECTIVE:To determine if estrogen ameliorates hot flashes by raising the core body temperature sweating threshold, by reducing core body temperature fluctuations, and/or by reducing sympathetic activation (as measured by plasma 3-methoxy-4-hydroxyphenylglycol). DESIGN:Laboratory physiological study. SETTING:Universi...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(01)03009-6
更新日期：2002-03-01 00:00:00
abstract：:Surgical alteration of rabbit oviducts was employed to investigate the mechanisms of ovum transport. Resection of a 5- to 10-mm segment of midampulla was followed by microsurgical end-to-end anastomosis. One to two months later, direct observations were made, in situ, of ampullary egg transport. Stained cumulus masses...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44421-3
更新日期：1979-12-01 00:00:00
abstract：OBJECTIVE:To assess the molecular signatures underlying endometrial disorder using cDNA microarray. DESIGN:Gene expression-based oligonucleotide array of the normal endometrium. SETTING:University hospital. PATIENT(S):Humans. INTERVENTION(S):Endometrial tissues were obtained from 28 normal cycling women undergoing ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.01.130
更新日期：2010-06-01 00:00:00
abstract：:Heterologous antibodies have frequently been used to develop assays in other species. Human urinary LH kits, widely available for predicting the LH surge, offer a unique opportunity to determine the timing of ovulation and mating in nonhuman primates. We tested three commercially available ovulation predictor kits for...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53301-9
更新日期：1990-02-01 00:00:00
abstract：OBJECTIVE:To measure autoantibodies that recognize oxidatively modified proteins in the sera of women with surgically proven endometriosis. DESIGN:Prospective study. SETTING:Tertiary care academic medical center. PATIENT(S):Women undergoing surgery for endometriosis or tubal ligation. INTERVENTION(S):None. MAIN OU...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00145-4
更新日期：1999-06-01 00:00:00
