当前位置： SCI文献检索 > FERTILITY AND STERILITY期刊下所有文献 > MicroRNA expression profiles in human testicular tissues of infertile men with different histopathologic patterns.

MicroRNA expression profiles in human testicular tissues of infertile men with different histopathologic patterns.

Abstract：

OBJECTIVE:To investigate the expression profiles of microRNA (miRNA) in human testes showing different histopathological patterns. DESIGN:Microarray with quantitative real-time polymerase chain reaction (qRT-PCR) validation. SETTING:University research and clinical institutes. PATIENT(S):Azoospermic men who underwent testicular biopsy for sperm recovery in preparation for intracytoplasmic sperm injection. INTERVENTION(S):Testicular biopsies. MAIN OUTCOME MEASURE(S):Statistically significantly altered miRNA expression profiles among the testicular histopathologic patterns groups compared with normal pattern group. RESULT(S):According to miRNA array, a total of 197, 68, and 46 miRNAs were found to be differentially expressed when comparing the samples from Sertoli cell only (SCO), mixed atrophy (MA), and germ cell arrest (GA) groups, respectively, with normal spermatogenesis (N). Five miRNAs have been validated using qRT-PCR, and the results were consistent with miRNA array analysis. Bioinformatics analysis showed that five microRNAs (hsa-mir-34b*, hsa-mir-34b, hsa-mir-34c-5p, hsa-mir-449a, and hsa-mir-449b*) were involved in apoptosis, cell proliferation, and differentiation. Notably, potential target genes of these five miRNAs were involved in the spermatogenesis process. CONCLUSION(S):This study provides new insights into specific miRNAs that are expressed in infertile men with different histopathologic patterns, suggesting a role of miRNAs in regulating male germ and somatic cells and that their alteration is associated with reproductive abnormalities.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Abu-Halima M,Backes C,Leidinger P,Keller A,Lubbad AM,Hammadeh M,Meese E

doi

10.1016/j.fertnstert.2013.09.009

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

78-86.e2

issue

1

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(13)03059-8

journal_volume

101

pub_type

杂志文章

相关文献

FERTILITY AND STERILITY文献大全
  • Sperm viability, apoptosis, and intracellular reactive oxygen species levels in human spermatozoa before and after induction of oxidative stress.

    abstract：OBJECTIVE:To investigate sperm viability, incidence of apoptosis, and intracellular basal and induced reactive oxygen species (ROS) in sperm fractions. DESIGN:Prospective controlled study. SETTING:Center for Reproductive Medicine at a tertiary care hospital. METHOD(S):Liquefied seminal ejaculates (n = 12) prepared b...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2008.10.068

    authors： Mahfouz RZ,du Plessis SS,Aziz N,Sharma R,Sabanegh E,Agarwal A

    更新日期：2010-02-01 00:00:00

  • Combined use of clomiphene and intranasal luteinizing hormone-releasing hormone for induction of ovulation in chronically anovulatory women.

    abstract：:Combined therapy with clomiphene and intranasal luteinizing hormone-releasing hormone (LHRH) was used to induce ovulation in eight chronically anovulatory patients under treatment for infertility. Clomiphene, 100 mg daily, was given from the 5th to the 9th day of the cycle. Synthetic LHRH was administered intranasally...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：

    authors： Phansey SA,Barnes MA,Williamson HO,Sagel J,Nair RM

    更新日期：1980-11-01 00:00:00

  • Cryptorchidism in the subfertile male.

    abstract：:Certain conclusions may be drawn from the present review and presentation of new data concerning the unilaterally cryptorchid patient and possible subfertility: 1. The truly undescended testis--not the retractile testis of infancy--will not descend spontaneously after 1 year of age. 2. The seminal quality of the unila...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(16)41889-3

    authors： Lipshultz LI

    更新日期：1976-06-01 00:00:00

  • Effect of freezing on the relative escape force of sperm as measured by a laser optical trap.

    abstract：OBJECTIVE:To determine the possible effect of freezing on sperm escape force as measured by laser trap. DESIGN:Controlled clinical study. SETTING:Normal volunteers, academic setting. PATIENTS:Normal, healthy volunteers. INTERVENTION:Sperm selection, freezing and thawing. Measurement of relative (sperm) escape force...

    journal_title：Fertility and sterility

    pub_type： 临床试验,杂志文章

    doi：

    authors： Dantas ZN,Araujo E Jr,Tadir Y,Berns MW,Schell MJ,Stone SC

    更新日期：1995-01-01 00:00:00

  • Radiozinc spermatogenesis test: follow-up semen analysis.

    abstract：:Semen analysis was performed on 8 men, every 2 months, for a period of 8 months, after the application of the radiozinc spermatogensis test. Semen was examined for volume, cell count, motility, and abnormal forms. Results showed no evidence of any changes in semen parameters. Student's test was used to compare the ...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(16)40037-3

    authors： el-Arini AF,Salama MN,el-Beheiry AH,el-Din Nofal MM

    更新日期：1973-11-01 00:00:00

  • Neuroendocrine response to an intravenous L-tryptophan challenge in women with premenstrual syndrome.

    abstract：OBJECTIVE:To evaluate the neuroendocrine responses to an intravenous L-tryptophan challenge across the menstrual cycle in women with premenstrual syndrome (PMS) and controls. DESIGN:Controlled clinical study. SETTING:The Clinical Research Center of an academic research environment. PATIENT(S):Women with PMS and heal...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(99)00452-5

    authors： Rasgon N,McGuire M,Tanavoli S,Fairbanks L,Rapkin A

    更新日期：2000-01-01 00:00:00

  • An interclass nuclear transfer between fowl and mammal: in vitro development of chicken-to-cattle interclass embryos and the detection of chicken genetic complements.

    abstract：:An attempt was made to develop an interclass somatic cell nuclear transfer method as an alternative means of establishing chicken embryonic stem cells. Chicken-to-cattle interclass embryos that activated calcium ionophore, cycloheximide, and cytochalasin D were developed into blastocysts, and the developing interclass...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2004.06.019

    authors： Kim TM,Park TS,Shin SS,Han JY,Moon SY,Lim JM

    更新日期：2004-10-01 00:00:00

  • A decade of experience emphasizes that testing for Y microdeletions is essential in American men with azoospermia and severe oligozoospermia.

    abstract：OBJECTIVE:To evaluate the benefit of Y microdeletion testing. DESIGN:Retrospective analysis. SETTING:University-based male fertility clinic and genetics laboratory. PATIENT(S):A total of 1,591 men with sperm concentrations less than 5 million sperm/mL. INTERVENTION(S):Semen analysis, Y microdeletion testing, microd...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2009.09.006

    authors： Stahl PJ,Masson P,Mielnik A,Marean MB,Schlegel PN,Paduch DA

    更新日期：2010-10-01 00:00:00

  • The desire of infertile patients for multiple births.

    abstract：OBJECTIVE:To determine the proportion of infertile women who prefer a multiple birth over a singleton, patient characteristics associated with this desire, and patient knowledge about the risks of multiple births. DESIGN:Prospective analysis. SETTING:Academic university hospital-based infertility center and private g...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2003.05.035

    authors： Ryan GL,Zhang SH,Dokras A,Syrop CH,Van Voorhis BJ

    更新日期：2004-03-01 00:00:00

  • The clinical value of salpingoscopy in tubal infertility.

    abstract：:A specially designed rigid salpingoscope has been developed to allow inspection of the tubal mucosa during laparoscopy. The presence and extent of intratubal adhesion formation can be evaluated more accurately by this technique than by either HSG or laparoscopy. In 22 patients with bilateral hydrosalpinges, an intraut...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(16)60501-0

    authors： De Bruyne F,Puttemans P,Boeckx W,Brosens I

    更新日期：1989-02-01 00:00:00

  • Efficacy of intravenous tramadol treatment for reducing pain during office diagnostic hysteroscopy.

    abstract：OBJECTIVE:To assess whether IV tramadol before outpatient hysteroscopy could reduce procedure-related pain. DESIGN:A randomized double-blind placebo controlled trial. SETTING:Outpatient Hysteroscopy Centre in the Department of Obstetrics and Gynaecology of Cagliari University. PATIENT(S):Fifty healthy, parous, women...

    journal_title：Fertility and sterility

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.fertnstert.2006.05.072

    authors： Floris S,Piras B,Orrù M,Silvetti E,Tusconi A,Melis F,Tuveri M,Piga M,Paoletti AM,Melis GB

    更新日期：2007-01-01 00:00:00

  • Parasitic leiomyoma in the abdominal wall after laparoscopic myomectomy.

    abstract：OBJECTIVE:To report a case of parasitic leiomyoma of the abdominal wall after laparoscopic myomectomy. DESIGN:Case report. SETTING:Large medical center. PATIENT(S):A 31-year-old woman with a newly developed palpable mass in the abdominal wall near the trocar site of a previous laparoscopic myomectomy. INTERVENTION(...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2007.08.068

    authors： Moon HS,Koo JS,Park SH,Park GS,Choi JG,Kim SG

    更新日期：2008-10-01 00:00:00

  • Different plasma neuropeptide Y concentrations in women athletes with and without menstrual cyclicity.

    abstract：:The circulating levels of leptin and neuropeptide Y, which are both involved in the control of feeding and reproduction, were measured in amenorrheic and normal cycling highly trained women athletes, and in normal cycling sedentary controls. Leptin showed similar low values in all athletes, whereas neuropeptide Y leve...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2005.08.041

    authors： Coiro V,Chiodera P,Melani A,Manfredi G,Saccani Jotti G,Casti A

    更新日期：2006-03-01 00:00:00

  • Significance of one pronucleus before fertilization.

    abstract：OBJECTIVE:To describe a case of primary infertility associated with oocytes having one pronucleus before fertilization on repeated IVF attempts. DESIGN:Case report. SETTING:A university-based assisted reproduction unit. PATIENT(S):A 30-year-old woman with primary infertility and oocytes containing one pronucleus bef...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(02)04852-5

    authors： Westphal LM,Rosencrantz M,Behr B,Milki AA

    更新日期：2003-04-01 00:00:00

  • Effects of exposure to high levels of particulate air pollution during the follicular phase of the conception cycle on pregnancy outcome in couples undergoing in vitro fertilization and embryo transfer.

    abstract：:The objective of this retrospective cohort study was to assess the potential effects of preconceptional short-term exposure to particulate air pollution in a real-world situation on pregnancy outcome in infertile women evaluating the possible role of IVF/embryo transfer treatment on this outcome using women who had co...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2009.06.031

    authors： Perin PM,Maluf M,Czeresnia CE,Nicolosi Foltran Januário DA,Nascimento Saldiva PH

    更新日期：2010-01-01 00:00:00

  • Proximal ectopic pregnancy: a descriptive general population-based study and results of different management options in 86 cases.

    abstract：OBJECTIVE:To describe different approaches for diagnosis and management of proximal ectopic pregnancies (PP) in general population. DESIGN:Observational population based-study. SETTING:Regional ectopic pregnancy registry. PATIENT(S):Eighty-six PP registered from 1992 to 2008. INTERVENTION(S):Surgical (radical or co...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2010.10.025

    authors： Larraín D,Marengo F,Bourdel N,Jaffeux P,Aublet-Cuvelier B,Pouly JL,Mage G,Rabischong B

    更新日期：2011-03-01 00:00:00

  • Mechanisms regulating invasiveness and growth of endometriosis lesions in rat experimental model and in humans.

    abstract：OBJECTIVE:To compare the expression of MMP-2, TIMP-2, and TGFbeta2 mRNA in experimental and human endometriotic lesions and to assess the possibility of its cytokine regulation. DESIGN:Experimental laboratory study. SETTING:Medical center. ANIMALS AND PATIENT(S): Thirty female Wistar rats, 17 women with endometriosis...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2009.11.024

    authors： Sotnikova NY,Antsiferova YS,Posiseeva LV,Shishkov DN,Posiseev DV,Filippova ES

    更新日期：2010-05-15 00:00:00

  • A preliminary concept, deduced from cytogenetic analyses, for explaining different types of multipronuclear oocytes obtained after intracytoplasmic sperm injection.

    abstract：:Oocytes with three or more pronuclei and one or two polar bodies arising after intracytoplasmic sperm injection were analyzed cytogenetically, and the data were used to develop a general model explaining the possible origin of the abnormal pronuclear stages. The mechanisms, either separately or in variable combination...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2010.03.075

    authors： Rosenbusch BE

    更新日期：2010-11-01 00:00:00

  • Recovery of microorganisms in semen and relationship to semen evaluation.

    abstract：:Semen samples from 120 consecutive unselected men attending our fertility clinic were studied to determine the bacterial isolation rate of semen and its influence on semen quality. Each sample was cultured aerobically, anaerobically, and for Ureaplasma urealyticum and Mycoplasma hominis. The following were analyzed fo...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(16)49105-3

    authors： Naessens A,Foulon W,Debrucker P,Devroey P,Lauwers S

    更新日期：1986-01-01 00:00:00

  • Recurrent implantation failure: gamete and embryo factors.

    abstract：:Chromosomal abnormalities, sperm DNA damage, zona hardening, inadequate culture conditions, and suboptimal embryo development all play a significant role in the etiology of recurrent implantation failure. Evidence suggests that preimplantation genetic screening does not increase implantation or live birth rates. Compa...

    journal_title：Fertility and sterility

    pub_type： 杂志文章,评审

    doi：10.1016/j.fertnstert.2012.02.029

    authors： Das M,Holzer HE

    更新日期：2012-05-01 00:00:00

  • Effects of letrozole on superovulation with gonadotropins in women undergoing intrauterine insemination.

    abstract：OBJECTIVE:To investigate the effects of letrozole in patients undergoing superovulation with gonadotropins and IUI. DESIGN:Retrospective analysis. SETTING:Academic teaching hospital. PATIENT(S):Women younger than 40 years of age with patent fallopian tubes and infertility of more than 1 year in duration who were und...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2003.03.001

    authors： Healey S,Tan SL,Tulandi T,Biljan MM

    更新日期：2003-12-01 00:00:00

  • Low-dose aspirin in reproductive health: effects on menstrual cycle characteristics.

    abstract：OBJECTIVE:To estimate the effect of daily 81 mg low-dose aspirin (LDA) on menstrual cycle length and hormone profiles. DESIGN:Secondary analysis of a trial evaluating the effect of daily LDA or placebo on live birth among women with one or two previous pregnancy losses. SETTING:University medical centers. PATIENT(S)...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2020.06.022

    authors： Evans MB,Nobles CJ,Kim K,Hill MJ,DeCherney AH,Silver RM,Mumford SL,Sjaarda LA,Perkins NJ,Schisterman EF

    更新日期：2020-12-01 00:00:00

  • Inhibition of G protein in human sperm and its influence on acrosome reaction and zona pellucida binding.

    abstract：OBJECTIVE:To evaluate human sperm acrosomal status, zona pellucida (ZP)-binding capacity, and sperm motion characteristics after treatment with pertussis toxin followed by exposure to increasing concentrations of solubilized human ZP. DESIGN:Prospective analytical study. SETTING:Normal human sperm donors in an academ...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：

    authors： Franken DR,Morales PJ,Habenicht UF

    更新日期：1996-12-01 00:00:00

  • Previous ectopic pregnancy as a predictor of failure of systemic methotrexate therapy.

    abstract：OBJECTIVE:To investigate whether a history of previous ectopic pregnancy is an independent risk factor for failure of methotrexate therapy in subsequent ectopic pregnancies and whether the previous treatment method influenced failure. SETTING:Inner-city teaching hospital. DESIGN:Retrospective case review. PATIENT(S)...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2003.09.070

    authors： Lipscomb GH,Givens VA,Meyer NL,Bran D

    更新日期：2004-05-01 00:00:00

  • Polycystic ovary syndrome is associated with negatively variable impacts on domains of health-related quality of life: evidence from a meta-analysis.

    abstract：OBJECTIVE:To systematically review the literature to identify the impact of polycystic ovary syndrome (PCOS) on specific health-related quality of life domains. DESIGN:Meta-analysis. SETTING:N/A. PATIENT(S):The outcomes of 423 patients and 285 controls from 5 articles that used the Short Form 36 (SF-36) questionnair...

    journal_title：Fertility and sterility

    pub_type： 杂志文章,meta分析,评审

    doi：10.1016/j.fertnstert.2011.05.072

    authors： Li Y,Li Y,Yu Ng EH,Stener-Victorin E,Hou L,Wu T,Han F,Wu X

    更新日期：2011-08-01 00:00:00

  • Serum levels of soluble vascular cell adhesion molecule-1, tumor necrosis factor-alpha, and interleukin-6 in in vitro fertilization cycles.

    abstract：OBJECTIVE:To assess whether gonadotropin-induced changes in E(2) alter serum levels of soluble vascular cell adhesion molecule-1 (sVCAM-1) and proinflammatory cytokines. DESIGN:Prospective collection of serum in patients undergoing IVF. SETTING:University hospital. PATIENT(S):Twenty-four infertile women. INTERVENTI...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2008.04.039

    authors： Souter I,Huang A,Martinez-Maza O,Breen EC,Decherney AH,Chaudhuri G,Nathan L

    更新日期：2009-05-01 00:00:00

  • Emerging role of embryo secretome in the paracrine communication at the implantation site: a proof of concept.

    abstract：OBJECTIVE:To assess the role of embryo secretome in modifying the molecular profile of glycodelin A (GdA) in endometrial organoids (ORG) mimicking the implantation window. To verify whether the use of embryo-conditioned culture medium at the time of the embryo transfer may increase in vitro fertilization outcome. DESI...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2020.10.058

    authors： Luddi A,Pavone V,Governini L,Capaldo A,Landi C,Ietta F,Paccagnini E,Morgante G,De Leo V,Piomboni P

    更新日期：2021-01-23 00:00:00

  • A suboptimal endometrial pattern is associated with a reduced likelihood of pregnancy after a day 5 embryo transfer.

    abstract：:This retrospective study examined the association of endometrial pattern and pregnancy after a day 5 ET. The pregnancy rate of women with a triple-line ultrasound endometrial pattern on the day of hCG administration was significantly higher than the pregnancy rate of women with the other ultrasound patterns. This obse...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2004.05.102

    authors： Potlog-Nahari C,Catherino WH,McKeeby JL,Wesley R,Segars JH

    更新日期：2005-01-01 00:00:00

  • Chromosomal analysis of meiotic stages of human oocytes matured in vitro: benefits of protease treatment before fixation.

    abstract：OBJECTIVE:To modify Tarkowski's air-dry technique for mouse oocytes to develop a rapid, consistent procedure for human oocytes that enables accurate scoring of meiotic stage. DESIGN, SETTING, AND PATIENTS:Meiotically immature human oocytes, obtained after oophorectomy, were cultured for various periods and then subjec...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/s0015-0282(16)55021-3

    authors： Racowsky C,Kaufman ML,Dermer RA,Homa ST,Gunnala S

    更新日期：1992-05-01 00:00:00

  • Influence of hysterectomy on long-term fracture risk.

    abstract：OBJECTIVE:To assess long-term fracture risk after hysterectomy, with or without oophorectomy. DESIGN:Population-based, cohort study. SETTING:Olmsted County, Minnesota. PATIENT(S):Women residing in Olmsted County (n = 9,258) who underwent hysterectomy in 1965-2002, compared to an equal number of age- and sex-matched ...

    journal_title：Fertility and sterility

    pub_type： 杂志文章

    doi：10.1016/j.fertnstert.2006.11.080

    authors： Melton LJ 3rd,Achenbach SJ,Gebhart JB,Babalola EO,Atkinson EJ,Bharucha AE

    更新日期：2007-07-01 00:00:00