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Emerging role of embryo secretome in the paracrine communication at the implantation site: a proof of concept.

Abstract：

OBJECTIVE:To assess the role of embryo secretome in modifying the molecular profile of glycodelin A (GdA) in endometrial organoids (ORG) mimicking the implantation window. To verify whether the use of embryo-conditioned culture medium at the time of the embryo transfer may increase in vitro fertilization outcome. DESIGN:Molecular study with human endometrial ORG and embryo-conditioned culture medium. Retrospective study using prospectively recorded data. SETTING:University hospital. PATIENT(S):For isolation and culture of endometrial glandular ORG, endometrial biopsy specimens from five white women of proven fertility undergoing laparoscopy for tubal sterilization. A total of 75 women undergoing intracytoplasmic sperm injection for tubal and/or male infertility factor. INTERVENTIONS(S):In vitro fertilization. MAIN OUTCOME MEASURE(S):Pinopodes presence in human endometrial ORG. Glycodelin A expression profile by means of two-dimensional electrophoresis. In vitro fertilization outcome. RESULT(S):This in vitro study demonstrated that the treatment of endometrial ORG with the secretome of medium conditioned by the growing embryo increased the GdA relative abundance and induced a different glycoform pattern. Biochemical and clinical pregnancy rate significantly increased when the spent medium was loaded during the transfer (17.5% vs. 36.6% and 16.5% vs. 35.1%, respectively). CONCLUSION(S):This study demonstrated that the secretome of implanting embryos is able to induce the expression as well as to determine the relative abundance and the glycosilation profile of endometrial GdA, a protein having a key role in the embryo-endometrial cross talk. Moreover, a significant increase in pregnancy rate was observed when the embryo transfer was performed by using the culture medium conditioned by the growing embryo.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Luddi A,Pavone V,Governini L,Capaldo A,Landi C,Ietta F,Paccagnini E,Morgante G,De Leo V,Piomboni P

doi

10.1016/j.fertnstert.2020.10.058

subject

Has Abstract

pub_date

2021-01-23 00:00:00

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(20)32630-3

pub_type

杂志文章

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Emerging role of embryo secretome in the paracrine communication at the implantation site: a proof of concept.