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Detection of the ovulatory-luteinizing hormone surge with an enzyme-linked immunospecific human urinary luteinizing hormone assay: applicability to nonhuman primates.

Abstract：

:Heterologous antibodies have frequently been used to develop assays in other species. Human urinary LH kits, widely available for predicting the LH surge, offer a unique opportunity to determine the timing of ovulation and mating in nonhuman primates. We tested three commercially available ovulation predictor kits for utility in two macaque species. Midfollicular, midcycle, and castrate urines and their corresponding serum samples were assessed for LH activity using established RIAs as well as urinary ovulation kits. Macaque LH did not sufficiently cross-react with the human urinary anti-LH antibodies in any of the predictor test kits. Although these kits offer excellent results in predicting ovulation by measurement of urinary LH in humans, they appear not to be applicable for urinary LH detection in rhesus nor cynomolgus macaques.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Simon JA,Esparcia-Fenwick LA,Hodgen GD

doi

10.1016/s0015-0282(16)53301-9

subject

Has Abstract

pub_date

1990-02-01 00:00:00

pages

372-4

issue

2

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)53301-9

journal_volume

53

pub_type

杂志文章

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