Isolated spermatogonia protrude active pseudopodia in vitro.

Abstract:

:When dispersed spermatogenic cells obtained by enzymatic digestion from prepuberal mice, adult male mice, nonazoospermic men and normospermic men were observed live using Normarski optics, it was found that, respectively, 47.4%, 1.4%, 5.1%, and 2.4% of them protruded active pseudopodia. These cells were 8 to 10 mum in diameter, had a high N/C ratio, and had one to two prominent nucleoli that were close to a distinct nuclear membrane. They showed low alkaline phosphatase activities and homogeneous nuclear immunoreactive patterns using gamma-H2AX, which suggests that they were spermatogonia.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Tanaka A,Nagayoshi M,Awata S,Himeno N,Tanaka I,Kusunoki H

doi

10.1016/j.fertnstert.2007.06.055

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

453-5

issue

2

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(07)01367-2

journal_volume

90

pub_type

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