Abstract：
OBJECTIVE:To investigate the usefulness of dual-parameter flow cytometry of testicular fine-needle aspirates for the quantification of spermatogenesis. Immunofluorescence staining for vimentin was introduced to discriminate vimentin-negative germ cells from vimentin-positive stromal cells. The results of flow cytometry were compared with testicular morphology and immunohistochemistry in cases of regular and disturbed germ cell maturation. DESIGN:Testicular fine-needle aspiration and surgical biopsy were performed in 50 autopsy cases. The fine-needle aspirates were double stained for the intermediate filament vimentin by indirect immunofluorescence and DNA propidium iodide and analyzed by flow cytometry. Surgical biopsies were examined by light microscopy. The distribution of vimentin-positive cells was evaluated by immunohistochemistry. RESULTS:A comparison of morphology and flow cytometric analysis yielded characteristic quantitative distribution patterns of the different germ cell and somatic cell populations in regular and disturbed spermatogenesis. All three ploidy compartments of the germ epithelium correlated with high statistical significance with the respective histologic diagnoses. Moreover, a marked quantitative increase of stromal cells could be demonstrated in spermatogenetic disorders. CONCLUSIONS:The simultaneous analysis of the cellular DNA content and the intermediate filament vimentin by flow cytometry enables a detailed investigation of spermatogenetic disorders. Quantitative changes of the relationships between germ cells and somatic cells can be selectively investigated.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Hittmair A,Rogatsch H,Mikuz G,Feichtinger Hdoi
10.1016/s0015-0282(16)56656-4subject
Has Abstractpub_date
1994-04-01 00:00:00pages
746-50issue
4eissn
0015-0282issn
1556-5653pii
S0015-0282(16)56656-4journal_volume
61pub_type
杂志文章abstract：OBJECTIVE:To compare ovarian performance and hormonal levels, after ovulation induction, in patients with isolated hypogonadotropic hypogonadism, using two different gonadotropin drugs. DESIGN:Patients were treated during consecutive cycles, using the same stimulation protocol, with human menopausal gonadotropin (hMG)...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)54715-3
更新日期：1991-12-01 00:00:00
abstract：:In this prospective case-control study, we determined the role of surface-enhanced laser desorption and ionization time-of-flight mass spectrometry in the detection of histologically proven endometriosis. In the plasma of the group with endometriosis, there were 20 different protein peaks, and the classifier showed a ...
journal_title：Fertility and sterility
pub_type： 信件
doi：10.1016/j.fertnstert.2006.08.095
更新日期：2007-04-01 00:00:00
abstract：OBJECTIVE:To measure the level of distress and its relationship with other psychologic factors in women with diminished ovarian reserve (DOR) who participated in a fragile X genetics study. DESIGN:Longitudinal data analyzed with structural equation modeling. SETTING:Four U.S. private and academic fertility centers. ...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究
doi：10.1016/j.fertnstert.2013.02.033
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVE:To determine the effect of sonographer experience during ultrasound guided embryo transfer on pregnancy outcomes. DESIGN:Retrospective chart review. SETTING:A university-based academic practice. PATIENT(S):A total of 319 women who underwent in vitro fertilization and embryo transfer (IVF-ET) from the site-...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.07.1743
更新日期：2009-09-01 00:00:00
abstract：:A case of coma due to embolization after HSG, using oil-soluble contrast medium is presented. The patient was in a comatose state 11 days and then spontaneously regained full consciousness. The safety of oil-contrast medium for HSG is questionable. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53537-7
更新日期：1990-05-01 00:00:00
abstract：OBJECTIVE:To prove that low-dose hCG alone can be clinically used to replace FSH-containing gonadotropins to complete controlled ovarian hyperstimulation (COH). DESIGN:Controlled, prospective, randomized study. SETTING:Academic center. PATIENT(S):Infertile patients who are candidates for assisted reproduction. INTE...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2005.02.036
更新日期：2005-08-01 00:00:00
abstract：OBJECTIVE:To study the direct effects of progesterone (P) and its antagonist RU486 (mifepristone) on sperm hyperactivation (HA) and acrosome reaction. DESIGN:Prospective evaluation of semen samples incubated in capacitation media with P and/or RU486. SETTING:University-affiliated tertiary care center. PATIENTS:Norma...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:A prospective study of carbohydrate metabolism was carried out on seven women by using an oral glucose tolerance stimulation test and measuring blood glucose and plasma insulin values over a 3-hour time period. The test was performed before and after 6 months of treatment with a "low-estrogen" oral contraceptive conta...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)42747-0
更新日期：1977-08-01 00:00:00
abstract：OBJECTIVE:To determine whether polymorphisms in the estrogen receptor (ER) alpha and beta genes are associated with endometriosis in a Japanese population. DESIGN:Association study. SETTING:University hospital. PATIENT(S):Japanese women diagnosed with endometriosis by laparotomy or laparoscopy. INTERVENTION(S):Dete...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.02.094
更新日期：2004-06-01 00:00:00
abstract：OBJECTIVE:To evaluate the efficiency of using a continuous (one-step) protocol with a single medium for the culture of human embryos in a time-lapse incubator (TLI). DESIGN:Prospective cohort study on sibling donor oocytes. SETTING:University-affiliated in vitro fertilization (IVF) center. PATIENT(S):Embryos from 59...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2015.11.038
更新日期：2016-03-01 00:00:00
abstract：:Activities of deoxyribonucleic acid (DNA) polymerase alpha, beta, and gamma were measured in extracts of testicular biopsy specimen obtained from 37 cases of male infertility with left varicocele and compared with those of 6 normal controls. It was observed that levels of DNA polymerase alpha, beta, and gamma were sig...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60318-7
更新日期：1988-11-01 00:00:00
abstract：OBJECTIVE:To examine the inter-relationships between inhibin, relaxin, steroid concentrations, estradiol (E2), progesterone (P), and gonadotropins in early pregnancy. DESIGN:Hormone concentrations in plasma were measured during the luteal phase of subjects who became pregnant (n = 58) or failed to become pregnant (n =...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55628-3
更新日期：1993-01-01 00:00:00
abstract：:The outcome of a series of pre-embryo transfers to 31 women with ovarian failure is described. Twenty six fertile women functioned as nonanonymous donors, providing oocytes for in vitro fertilization after undergoing controlled ovarian hyperstimulation and transvaginal ultrasound directed oocyte aspiration. Recipients...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54055-2
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:Racial disparity in assisted reproductive technology (ART) outcomes has been reported but remains controversial. Reasons for the disparity are unclear, and access to care has been suggested as a causative factor. In this study, we sought to examine minority utilization of ART in the Department of Defense (DoD...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.10.028
更新日期：2006-04-01 00:00:00
abstract：OBJECTIVE:To determine if endometrial tissue specific complement (C) activation occurs in patients with endometriosis. DESIGN:Prospective. SETTING:University of Oklahoma Health Sciences Center, a tertiary care referral center. PATIENTS:Twenty-six women, 9 with endometriosis and 17 without endometriosis. INTERVENTIO...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:In this prospective, randomized clinical trial, we tested the hypothesis that a needle-free CO(2) injection system (Biojector 2000) would be better tolerated by patients when compared with traditional sharp needles used for gonadotropin injections during stimulation for IVF. As measured by our questionnaire, the needl...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2009.03.013
更新日期：2009-10-01 00:00:00
abstract：OBJECTIVE:To compare the clinical and endocrinologic effects of cyproterone acetate (CPA), an antiandrogen with progestational activity; flutamide, a nonsteroidal antiandrogen, and finasteride, an inhibitor of 5alpha-reductase. DESIGN:Randomized, open, controlled clinical study. SETTING:Department of Obstetrics and G...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(98)00486-5
更新日期：1999-03-01 00:00:00
abstract：OBJECTIVE:To assess the impact on women's reproductive outcomes of barbed sutures to repair uterine breaches during laparoscopic myomectomy compared with traditional smooth sutures. DESIGN:Retrospective, monocentric cohort study, with information on subsequent pregnancies prospectively acquired for some women. SETTIN...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.07.036
更新日期：2021-01-01 00:00:00
abstract：:A patient with amenorrhea due to a prolactin-secreting pituitary microadenoma was treated with the antiserotoninergic drug metergoline for 8 months. The first menstruation occurred after 1 month of therapy, and it was followed by regular menses by the 3rd month. Presumptive evidence of ovulation was obtained in at lea...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43466-7
更新日期：1978-08-01 00:00:00
abstract：:Buserelin acetate, a luteinizing hormone-releasing hormone agonist, is known to be effective in the shrinkage of uterine fibroids. A prospective trial was undertaken (1) to compare the efficacy of intranasal (IN) and subcutaneous (SC) administration of buserelin acetate and (2) to assess if tumor regression correlated...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)54001-1
更新日期：1990-12-01 00:00:00
abstract：:Surgical alteration of rabbit oviducts was employed to investigate the mechanisms of ovum transport. Resection of a 5- to 10-mm segment of midampulla was followed by microsurgical end-to-end anastomosis. One to two months later, direct observations were made, in situ, of ampullary egg transport. Stained cumulus masses...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44421-3
更新日期：1979-12-01 00:00:00
abstract：:Luteal phase defect (LPD) accounts for a significant proportion of reproductive disorders, however its etiology is still debated. A prospective study was performed on 37 ovulatory women to determine whether LPD can occur in cycles characterized by completely normal folliculogenesis. Criteria for normal folliculogenesi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53152-5
更新日期：1989-12-01 00:00:00
abstract：OBJECTIVE:To report the use of cumulative live-birth rates (CLBRs) per ovarian stimulation cycle to measure the success of IVF is proving to be the most accurate method for advising couples who failed to conceive, although the accuracy yielded is relatively low, and cycle outcome is highly dependent on the number of em...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.05.008
更新日期：2011-07-01 00:00:00
abstract：OBJECTIVE:To assess effects of estrogen pretreatment in GnRH antagonist protocol. DESIGN:Prospective, randomized multicenter study. SETTING:Ten private or university-based centers. PATIENT(S):A total of 472 patients undergoing IVF/ICSI. INTERVENTION(S):Randomization by sealed envelopes to receive 17β-estradiol (4 m...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.fertnstert.2012.02.028
更新日期：2012-06-01 00:00:00
abstract：:After four unsuccessful attempts at multiple follicular induction with exogenous gonadotropins, a patient underwent two attempts at IVF in spontaneous cycles triggered with hCG. The second attempt resulted in the transfer of three embryos and a successful singleton intrauterine pregnancy. We believe that unstimulated ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60746-x
更新日期：1989-06-01 00:00:00
abstract：OBJECTIVE:To study the in vivo mechanisms of action of ulipristal acetate (UPA) on uterine myomas. DESIGN:Retrospective histologic and immunohistochemical (IHC) study of myomas. SETTING:Academic research unit. PATIENT(S):Among 59 women with symptomatic myomas who underwent myomectomy, 42 were treated preoperatively ...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2015.04.025
更新日期：2015-08-01 00:00:00
abstract：:Many women now choose to develop their careers before having children. Thus, it is becoming increasingly important to assess a woman's potential for extended fertility and to understand the health consequences of having children at a late age. In particular, there is a striking positive correlation between extended fe...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2015.02.008
更新日期：2015-05-01 00:00:00
abstract：OBJECTIVE:Partially damaged frozen and thawed embryos are currently considered to have a lower viability than intact ones. This study was undertaken to compare the performance of intact frozen and thawed embryos with that of partially damaged embryos after removal of the necrotic blastomeres. DESIGN:Observational clin...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.04.038
更新日期：2005-10-01 00:00:00
abstract：:Chronic treatment with highly active analogs of luteinizing hormone (LH)-releasing hormone (LH-RH) induces "paradoxical" antifertility effects. Intact male rats, a reduction in testosterone production is observed after the administration of [D-Ser(But)6]-LH-RH(1-9)ethylamide (burserelin, Hoe 766), 50 ng/day subcutaneo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)43461-8
更新日期：1978-08-01 00:00:00
abstract：OBJECTIVE:To evaluate the association between female cystic fibrosis (CF) carrier status and in vitro fertilization (IVF) response and outcomes. The presence of cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations in male carriers has been associated with infertility, yet possible adverse effects o...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.07.1234
更新日期：2014-11-01 00:00:00
