Abstract:
OBJECTIVE:To study the direct effects of progesterone (P) and its antagonist RU486 (mifepristone) on sperm hyperactivation (HA) and acrosome reaction. DESIGN:Prospective evaluation of semen samples incubated in capacitation media with P and/or RU486. SETTING:University-affiliated tertiary care center. PATIENTS:Normal healthy volunteers. INTERVENTIONS:Semen samples were incubated in media with P or RU486 alone or in combination, and aliquots were taken at 10 minutes, 1, 5, and 24 hours for HA analyses by computer-aided sperm analysis system, and at 0, 5, and 24 hours for assessment of acrosome reaction by fluorescein-labeled Pisum sativum (pea) agglutinin. MAIN OUTCOME MEASURES:HA and acrosome reaction. RESULTS:Sperm HA was significantly increased at 10 minutes by P both at 10(-7) M (9.27 +/- 1.59%; mean +/- SEM) and 10(-6) M (9.39 +/- 1.94%) when compared with untreated spermatozoa (5.62 +/- 1.59%). The stimulatory effect of P on sperm HA was transient because this was not observed after 1, 5, and 24 hours of incubation. The antiprogesterone RU486 (10(-6) M) alone had no effect and did not abolish the stimulatory effect of P on HA. The %HA was further enhanced by the addition of RU486 at 10(-6) M to P at 10(-7) M (12.43 +/- 3.31%) or P at 10(-6) M (13.52 +/- 4.10%); however, this effect was not significantly different from P alone. Coincubation of P or RU486 with spermatozoa during capacitation did not stimulate the acrosome reaction in the concentrations tested. CONCLUSION:Progesterone directly stimulates human sperm HA transiently. Progesterone has no significant effect on acrosome reaction in capacitating spermatozoa. The effects of P are rapid and not counteracted by RU486, suggesting that the mechanism of action of P may not be mediated by specific P nuclear receptors. :Reproductive endocrinologists at the University of California, Los Angeles Medical Center, obtained semen samples from healthy volunteers to examine the direct effects of progesterone and the antiprogesterone RU-486 on sperm hyperactivation and acrosome reaction, which they hoped would elucidate progesterone's mechanism of action. They used progesterone concentrations which match those of the female reproductive tract. Progesterone induced a considerable increase in sperm hyperactivation at 10 millionths M and 1 millionth M after 10 minutes (9.27% and 9.39% vs. 5.62% for untreated spermatozoa; p .05). The ability of progesterone to increase sperm hyperactivation was only temporary, however. In fact, progesterone had no effect at 1, 5, and 24 hours. RU-486 alone did not affect sperm hyperactivation. Addition of RU-486 at 1 millionth M to progesterone further did strengthen the stimulatory effect, however, but it was not significant (13.52% vs. 9.39%). When progesterone or RU-486 were coincubated with spermatozoa during the process by which they become able to fertilize the ovum after they are at the ampullar portion of the uterine tube, neither chemical stimulated the acrosome reaction, regardless of the concentration. These results indicated that progesterone can only directly stimulate sperm hyperactivation temporarily and rapidly (within about 10 minutes) and does not influence acrosome reaction during capacitation. They also demonstrated that RU-486 cannot counteract progesterone. Thus, specific progesterone receptors do not mediate progesterone's mechanism of action.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Uhler ML,Leung A,Chan SY,Wang Ckeywords:
subject
Has Abstractpub_date
1992-12-01 00:00:00pages
1191-8issue
6eissn
0015-0282issn
1556-5653pii
S0015-0282(16)55568-Xjournal_volume
58pub_type
杂志文章abstract::The third annual meeting of the Androgen Excess Society, held in San Diego, California, June 3, 2005, reported on new developments in the field, including intrafollicular steroidogenesis, intrathecal biochemistry, mechanisms of insulin signal and resistance of polycystic ovary syndrome, and novel aspects of androgen s...
journal_title:Fertility and sterility
pub_type:
doi:10.1016/j.fertnstert.2006.06.049
更新日期:2006-11-01 00:00:00
abstract::Semen analyses were performed and serum and seminal plasma prolactin (PRL) concentrations were determined in 165 samples from 120 men seen with their wives because of infertility. The mean (+/- standard deviation) serum and seminal plasma PRL concentrations were 6.5 +/- 3.3 and 7.5 +/- 3.1 ng/ml, respectively. The mea...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59664-2
更新日期:1988-01-01 00:00:00
abstract:OBJECTIVE:To determine whether complete failure of fertilization of oocytes in couples with unexplained infertility persists during subsequent in vitro fertilization (IVF) cycles. DESIGN:A retrospective study of 120 cycles of IVF in 44 couples with unexplained infertility and complete failure of fertilization during t...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)55677-5
更新日期:1993-02-01 00:00:00
abstract::A patient with primary adrenocortical deficiency presented with galactorrhea and hyperprolactinemia, in addition to the usual characteristic symptoms of adrenal insufficiency. With steroid replacement therapy, PRL levels returned to normal, with resumption of normal ovulatory cycles; and the patient became pregnant. P...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48872-2
更新日期:1985-09-01 00:00:00
abstract:OBJECTIVE:To analyze the effectiveness of autologous endometrial coculture by the cycle day of the endometrial biopsy. DESIGN:Retrospective study. SETTING:University-based IVF center. PATIENT(S):Two hundred eight patients with multiple IVF failures. INTERVENTION(S):Embryos were split and randomly allocated to growt...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(02)03134-5
更新日期:2002-06-01 00:00:00
abstract:OBJECTIVE:To investigate the expression and localization of interleukin-8 (IL-8) and monocyte chemotactic protein 1 (MCP-1) in women with and without endometriosis. DESIGN:Comparative immunohistochemical study. SETTING:Academic medical center. PATIENT(S):Ectopic (n = 24) and homologous eutopic endometrium (n = 24) f...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.12.067
更新日期:2009-03-01 00:00:00
abstract:OBJECTIVE:To assess the potential involvement of vascular endothelial growth factor in the hyperpermeability characterizing the ovarian hyperstimulation syndrome (OHSS). DESIGN:A controlled clinical study that followed the kinetics of vascular endothelial growth factor in the plasma of patients with severe OHSS from t...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/S0015-0282(97)81908-5
更新日期:1997-02-01 00:00:00
abstract::In 115 patients (group I) in which normal liquefaction of semen occurred, the fertilization rate of the yielded oocytes was 71.2% after standard in vitro fertilization procedure, and the viable pregnancy rate per patient per cycle was 13.0%. In a comparable group of 26 patients (group II), in which semen liquefaction ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60444-2
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the effect of frozen, compared with fresh, embryo transfer on neonatal and pediatric weight and weight gain trajectory. DESIGN:Retrospective cohort study. SETTING:Academic medical center. PATIENT(S):Women who underwent fresh or frozen embryo transfer at the Mayo Clinic from 2010 to 2014. All in...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2019.05.020
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVE:Mutation within androgen receptor (AR) can be seen. Presently, prediction of protein nanostructure and function is a great challenge in the proteomics and structural genomics era. To identify the points vulnerable to mutation is a new trend to expand the knowledge on disorders in genomic and proteomic level o...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.06.089
更新日期:2009-01-01 00:00:00
abstract::Paternal aging is associated with increased risk of genetic disease transmission to the offspring. The changes associated with aging arise predominantly through formation of single nucleotide variation through DNA replication errors, as well as possibly chronic exposure to environmental toxins and reactive oxygen spec...
journal_title:Fertility and sterility
pub_type: 杂志文章,评审
doi:10.1016/j.fertnstert.2016.12.021
更新日期:2017-02-01 00:00:00
abstract:OBJECTIVE:To examine the relationship between an antibody against GAPDH-2, a sperm-specific protein, and infertility of female mice. DESIGN:Basic research. SETTING:National Research Institute for Family Planning Beijing, World Health Organization Collaboration Center of Human Reproduction. ANIMAL(S):New Zealand rabb...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.09.001
更新日期:2009-12-01 00:00:00
abstract::Multiple factors influence the outcome of in vitro fertilization and embryo transfer (IVF-ET). This prospective study was designed to assess their relative importance, in order to improve prognostic ability and treatment success. Prior to IVF-ET, couples were divided into "good" (GP) and "poor" (PP) prognosis groups a...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60676-3
更新日期:1989-05-01 00:00:00
abstract:OBJECTIVE:To determine whether female body mass index (BMI) is associated with an increased risk of miscarriage after euploid embryo transfer. DESIGN:A retrospective, observational, multicenter cohort study. SETTING:University-affiliated in vitro fertilization center. PATIENT(S):In this study, 3,480 cycles of in vit...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2020.09.139
更新日期:2020-11-30 00:00:00
abstract:OBJECTIVE:To examine the expression of the heat shock protein hsp70-2, and the possible relationship with the pathogenesis of male infertility. DESIGN:Prospective study. SETTING:Reproductive testing laboratory in a university hospital. PATIENT(S):Men undergoing testicular biopsy during an investigation of subfertili...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(01)02892-8
更新日期:2001-12-01 00:00:00
abstract:OBJECTIVE:To examine the literature systematically in order to identify prospective comparative trials answering the following question: Is vitrification of human embryos associated with a higher postthawing survival rate as compared with slow freezing? DESIGN:Systematic review and meta-analysis. SETTING:University-b...
journal_title:Fertility and sterility
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.fertnstert.2007.06.010
更新日期:2008-07-01 00:00:00
abstract:OBJECTIVE:To study attitudes about posthumous assisted reproduction (PAR) in individuals presenting for assisted reproduction, to examine the degree of concordance of attitudes within couples, and to determine whether individuals can accurately predict the attitudes of their intimate partners. DESIGN:Cross sectional s...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.09.018
更新日期:2011-12-01 00:00:00
abstract::The pituitary response to luteinizing hormone-releasing hormone (LHRH) was tested in women before and approximately 2, 8, and 12 weeks after the injection of either Depo-Provera or norethisterone oenanthate. The secretion of both luteinizing hormone and follicle-stimulating hormone in response to LHRH was similar in t...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)43635-6
更新日期:1978-11-01 00:00:00
abstract:OBJECTIVE:To compare fecundity rates following intrauterine insemination (IUI) with donor sperm frozen conventionally versus an IUI-ready preparation. DESIGN:Both retrospective results and a prospective, randomized study where recipients were assigned to one of two sperm cryopreservation methods in each cycle of intra...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0015-0282(01)01857-x
更新日期:2001-07-01 00:00:00
abstract:OBJECTIVE:To determine CA-125 levels in cervical mucus (CM) during the menstrual cycle and their relationship to gonadal steroids and ovulation. DESIGN:Prospective study. SETTING:Two academic tertiary referral centers. PARTICIPANTS:Thirteen women with a normal fertility work-up. INTERVENTIONS:CA-125 and protein con...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)56694-1
更新日期:1994-05-01 00:00:00
abstract:OBJECTIVE:To report a rare complication of GnRH agonist therapy for intestinal endometriosis. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 45-year-old nulliparous Japanese woman with catamenial digestive symptoms. INTERVENTION(S):GnRH agonist therapy. MAIN OUTCOME MEASURE(S):Acute abdomenal crisis...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2006.12.082
更新日期:2007-10-01 00:00:00
abstract::The purpose of this study was to determine the effect of four different assisted hatching techniques on pregnancy rates in women with prior IVF failure in fresh IVF cycles. The results suggested that assisted hatching utilizing laser, chemical, or microsurgical techniques increases both implantation and pregnancy rate...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.02.133
更新日期:2009-04-01 00:00:00
abstract::Luteinization of an unruptured ovarian follicle has been reported as a cause of infertility and has heretofore been diagnosed only by direct visualization of the ovarian surface. Four patients who demonstrated defects in ovulation requiring therapy to induce ovulation and who were not achieving desired conception desp...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)46160-1
更新日期:1982-04-01 00:00:00
abstract::Twelve human uteri containing intrauterine contraceptive devices (IUDs) and ten uteri without IUDs were obtained at hysterectomy. Samples of fluid were collected from the uterine lumina by absorbing the fluid in small pieces of lens paper. In the samples of luminal fluid we measured the concentration of beta-galactosi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)42078-9
更新日期:1976-09-01 00:00:00
abstract:OBJECTIVE:To clarify the role of ovarian steroids in the development and progression of endometriosis, estrogen receptors (ERs) and progesterone receptors (PRs) were localized by immunohistochemistry, and ER messenger RNA (mRNA) was detected by in situ hybridization in the uterine endometrium and in normal and altered ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(97)81397-0
更新日期:1997-05-01 00:00:00
abstract:OBJECTIVE:To determine association of cytokine gene polymorphism with risk for recurrent miscarriages (RM). DESIGN:Retrospective case-control study on northern Indian RM cases versus control subjects. SETTING:Medical facility. PATIENT(S):A total of 200 women with at least three unexplained spontaneous abortions befo...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.09.025
更新日期:2013-02-01 00:00:00
abstract::Blood glucose and plasma insulin levels were measured during an oral glucose tolerance test in 18 women before and after 3 months' use of a low-estrogen oral contraceptive containing 0.035 mg of ethinylestradiol and 0.5 mg of norethindrone. There was no significant change in either of these two metabolic parameters. I...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)45620-7
更新日期:1981-07-01 00:00:00
abstract:OBJECTIVE:To determine the effect of moderate alcohol intake on probability of conception. DESIGN:Prospective cohort study. SETTING:Normal healthy women enrolled in an artificial donor insemination program. PATIENTS:Women starting artificial donor insemination for the very first time were selected. INTERVENTIONS:Al...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)57056-3
更新日期:1994-11-01 00:00:00
abstract::Seven homozygotes for F-XIII deficiency were found in a large inbred Israeli-Arab kindred. One affected woman had early abortions. Two affected men and one probably affected man had children, two of whom also were affected. Paternity was confirmed by segregation of HLAs and erythrocyte antigens and enzymes. This is th...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)49351-9
更新日期:1986-05-01 00:00:00
abstract:OBJECTIVE:To evaluate the effectiveness of progestogen supplementation in improving clinical pregnancy rates in women undergoing fresh IVF cycles and to compare different routes, start times, durations, and estrogen coadministration regimen. DESIGN:Comprehensive systematic review and meta-analysis. SETTING:University...
journal_title:Fertility and sterility
pub_type: 杂志文章,meta分析
doi:10.1016/j.fertnstert.2019.04.021
更新日期:2019-09-01 00:00:00