Abstract：
:The effects of freezing and thawing (F/T) on functional activity of immature rat oocyte-cumulus complexes (OCC) were studied. The OCC were divided into three groups according to the number of cumulus-cell layers surrounding them. The OCC were then frozen and thawed (F/T), with dimethyl sulfoxide (DMSO) as cryoprotectant. The survival rates after thawing increased significantly (P less than 0.001) as the number of cumulus-cell layers increased. Germinal vesicle breakdown (GVBD) was evaluated in F/T oocytes. After 2 hours, significantly (P less than 0.05) fewer oocytes demonstrated GVBD than did those in the control group. There was no difference, however, after 4 hours of culture. A significant (P less than 0.05) decrease in follicle-stimulating hormone (FSH)-dependent cyclic adenosine monophosphate (cAMP) accumulation was observed in the F/T group. However, the amount of cAMP produced was sufficient to maintain the oocyte in meiotic arrest. There was a borderline significant decrease of the coupling between cumulus cells and the oocyte in F/T OCC, as evaluated by the transport of 3H-uridine into the oocyte. It is concluded that immature rat oocytes can be successfully cryopreserved when they are surrounded by five or more layers of cumulus cells. FSH responsiveness and intercellular communication were essentially maintained. There was a slight delay in GVBD, which needs further clarification.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Pellicer A,Lightman A,Parmer TG,Behrman HR,De Cherney AHsubject
Has Abstractpub_date
1988-11-01 00:00:00pages
805-10issue
5eissn
0015-0282issn
1556-5653pii
S0015-0282(16)60320-5journal_volume
50pub_type
杂志文章abstract：:We prospectively evaluated the behavior of serum 17-hydroxyprogesterone (17-OHP), sex-steroid hormones, and C-reactive protein (CRP) levels in 27 patients during controlled ovarian hyperstimulation (COH) for in vitro fertilization (IVF). While routine measurement of COH via serum 17-OHP may replace estradiol (E(2)) an...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.05.024
更新日期：2008-05-01 00:00:00
abstract：OBJECTIVE:To investigate whether Kremer's sperm-mucus penetration test may predict sperm fertilizing ability in IVF. DESIGN:Kremer's test was prospectively performed on semen samples used for 66 consecutive IVF trials and compared with the fertilization rates and fertilization failure rates observed. RESULTS:Fertiliz...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55981-0
更新日期：1993-06-01 00:00:00
abstract：OBJECTIVE:To determine the probability of detecting the gestational sac of a normal intrauterine pregnancy by vaginal ultrasound at different gestational ages and serum hCG concentrations. DESIGN:Prospective, randomized study. SETTING:Pregnant human volunteers in a university-based clinical research environment. PAT...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)56749-1
更新日期：1994-06-01 00:00:00
abstract：OBJECTIVE:To determine whether high levels of interleukin (IL)-10 can attenuate the production of tumor necrosis factor (TNF)-alpha-induced proinflammatory cytokines in endometriotic stromal cells. DESIGN:Prospective study. SETTING:Department of Ob/Gyn, Tottori University, Japan. PATIENT(S):Thirty-five patients with...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.04.052
更新日期：2009-05-01 00:00:00
abstract：OBJECTIVE:To evaluate the occurrence of mutations of 5alpha-reductase type 2 gene in patients with cryptorchidism or idiopathic azoospermia. DESIGN:Controlled clinical study. SETTING:Yamagata University Hospital, Yamagata and Tokyo Electric Power Company Hospital, Tokyo, Japan. PATIENT(S):Patients with isolated cryp...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(02)03220-x
更新日期：2002-08-01 00:00:00
abstract：:Luteal phase defect (LPD) accounts for a significant proportion of reproductive disorders, however its etiology is still debated. A prospective study was performed on 37 ovulatory women to determine whether LPD can occur in cycles characterized by completely normal folliculogenesis. Criteria for normal folliculogenesi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53152-5
更新日期：1989-12-01 00:00:00
abstract：OBJECTIVE:To investigate the localization of tenascin expression in the endometrium of women without endometriosis and in endometriotic implants, and to determine the in vitro regulation of tenascin by E(2) in these tissues. DESIGN:Experimental laboratory study. SETTING:University medical center. PATIENT(S):Reproduc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.05.028
更新日期：2008-05-01 00:00:00
abstract：OBJECTIVE:To evaluate the outcome of repeated electroejaculation for obtaining semen from adolescents and young men before initiation of anticancer therapies. DESIGN:Retrospective clinical study. SETTING:Bikur Cholim Hospital, Jerusalem, Israel. PATIENT(S):Six young male patients (average age, 18+/-3 years) with dia...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)01680-6
更新日期：2001-04-01 00:00:00
abstract：:We wished to verify whether semen processing by discontinuous double-layered density gradient centrifugation could improve sperm apoptotic DNA fragmentation rates using a commercially available deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay in 35 consecutive men presenting for ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.01.059
更新日期：2008-09-01 00:00:00
abstract：:In this study of 132 infertile couples, findings with hysterosalpingography (HSG) were compared with those observed at subsequent culdoscopy or laparoscopy. Both procedures correctly revealed normal tubes in 29% of the subjects and identical abnormalities in 24%. A complete agreement between the two procedures was thu...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)41531-1
更新日期：1975-12-01 00:00:00
abstract：:Possible mechanisms of action of a combination of ethinylestradiol (EE) and dl-norgestrel as a postcoital contraceptive agent were studied in 12 healthy female volunteers. An oral dose of 0.1 mg of EE and 1.0 mg of dl-norgestrel was given at the predicted time of ovulation and again 12 hours later. Serum luteinizing h...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44237-8
更新日期：1979-09-01 00:00:00
abstract：OBJECTIVE:To evaluate whether the developmental potential of the blastocyst is affected by the number of trophectoderm (TE) cells biopsied in preimplantation genetic diagnosis (PGD) cycles. DESIGN:Retrospective study. SETTING:University-affiliated center. PATIENT(S):Women underwent PGD cycles of blastocyst biopsy an...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2016.01.011
更新日期：2016-05-01 00:00:00
abstract：OBJECTIVE:To investigate whether components of the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway are present and active in human sperm. DESIGN:Comparative study. SETTING:Reproductive biology department. PATIENT(S):Nine sperm donors. INTERVENTION(S):Sperm were exposed to interfer...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)01896-9
更新日期：2001-08-01 00:00:00
abstract：OBJECTIVE:To determine the optimal GnRH agonist dose for triggering of oocyte maturation in oocyte donors. DESIGN:Single-center, randomized, parallel, investigator-blinded trial. SETTING:IVFMD, My Duc Hospital, Ho Chi Minh City, Vietnam. PATIENT(S):One hundred sixty-five oocyte donors (aged 18-35 years, body mass in...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2015.10.014
更新日期：2016-02-01 00:00:00
abstract：:Pieces of polyurethane, Teflon, silicone, and polyvinyl chloride were placed intraperitoneally to compare the histologic findings regarding intraperitoneal adhesion development in a rat model. The overall adhesion score differed significantly among the groups, the highest being in the Teflon group (mean 13.2), followe...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.10.012
更新日期：2005-02-01 00:00:00
abstract：:The use of modified natural-cycle in vitro fertilization (IVF) is a valuable alternative to controlled ovarian hyperstimulation in young poor responders and should be considered in patients who require IVF and demonstrate endocrinologic evidence of ovarian aging and in those who have had one or two canceled controlled...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.09.052
更新日期：2011-11-01 00:00:00
abstract：:Seven nonionic detergents of the Igepal CO series differing in molecular size and including nonoxynol-9 and a representative anionic (SDS) and cationic (Cepacol) detergent were tested as to their relative cytotoxicity. The biologic effects of these detergents on human W1--38 fibroblasts (DNA and glycosaminoglycans syn...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1980-05-01 00:00:00
abstract：:This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/about/our-business/policies/article-withdrawal). This article has been retracted at the request of the Authors. The entire group of investigators contributing to this study requests to withdraw this article. We ...
journal_title：Fertility and sterility
pub_type： 杂志文章,撤回出版物
doi：10.1016/j.fertnstert.2020.09.001
更新日期：2020-11-01 00:00:00
abstract：:A couple may be considered to have fertility problems if they have been trying to conceive for over 1 year with no success. Worldwide, the inability to have children affects 10% to 15% of all couples. Subfertility can be divided into either male or female factor, or both partners can be affected. However, for some cou...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2018.05.028
更新日期：2018-09-01 00:00:00
abstract：OBJECTIVE:To present a case report of a unilateral triplet ectopic pregnancy (EP) conceived by IVF-embryo transfer. DESIGN:Case report. SETTING:University Hospital, Budapest, Hungary. PATIENT(S):A 26-year-old infertile woman with a history of right salpingectomy, hyperprolactinemia, and male factor infertility under...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.03.018
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVE:To describe a clinical approach to and outcomes of IVF in reproductive-aged cancer survivors receiving targeted cancer therapies. DESIGN:Case report. SETTING:Not applicable. PATIENT(S):The first case is of a female patient with metastatic lung cancer receiving long-term crizotinib, an anaplastic lymphoma k...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2016.08.011
更新日期：2016-11-01 00:00:00
abstract：OBJECTIVE:To determine whether patients who failed an in vitro fertilization (IVF) cycle can proceed with a subsequent IVF cycle after waiting only one menstrual cycle, or whether there is a benefit to allowing two or more menstrual cycles to elapse before proceeding. DESIGN:Retrospective cohort study. SETTING:Academ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.11.044
更新日期：2013-04-01 00:00:00
abstract：OBJECTIVE:To determine the expression profiles of the DAZ gene family in men with and without spermatogenic failure. DESIGN:Prospective case study. SETTING:University-based reproductive clinics and genetics laboratory. PATIENT(S):Thirty-four infertile men presenting with azoospermia. INTERVENTION(S):The mRNA transc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.08.047
更新日期：2004-04-01 00:00:00
abstract：OBJECTIVE:To estimate the prevalence and the characteristics of intrauterine adhesions after cold loop resectoscopic myomectomy. DESIGN:Retrospective study. SETTING:Endoscopic gynecologic center. PATIENT(S):688 women with one or more G1-G2 myomas. INTERVENTION(S):Cold loop resectoscopic myomectomy and diagnostic hy...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.09.032
更新日期：2014-01-01 00:00:00
abstract：:The result of the hormone concentration of one blood sample was used to determine the accuracy of predicting not only the hormone concentrations of a second and third sample drawn 15 minutes apart, but also the mean value of the three samples. Three blood specimens from 73 men involved in two previously reported studi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)59662-9
更新日期：1988-01-01 00:00:00
abstract：OBJECTIVE:To determine if men with malignancy have increased sperm DNA fragmentation compared with men presenting for sperm donation. DESIGN:Retrospective observational study. SETTING:Tertiary-level fertility center. PATIENT(S):Eighty-nine men with cancer presenting for prophylactic semen cryopreservation and 35 men...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.02.015
更新日期：2013-06-01 00:00:00
abstract：OBJECTIVE:To examine the expression and localization of Ets-1 in the rat ovary during the estrous cycle and pregnancy, and to investigate its effects on ovarian function. DESIGN:Prospective, randomized study. SETTING:Department of Physiology at Harbin Medical University. ANIMAL(S):Pubertal female Wistar rats. INTER...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.02.166
更新日期：2009-05-01 00:00:00
abstract：OBJECTIVE:To examine the impact of a recent surgery on development of endometriosis-related adhesions in a chimeric model and to determine the therapeutic efficacy of pioglitazone (PIO). DESIGN:Human endometrial biopsies were maintained in E(2) with or without PIO for 24 h before intraperitoneal injection into immunoc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.09.017
更新日期：2011-03-15 00:00:00
abstract：OBJECTIVE:To present a case of a unilateral endometrioma with extremely elevated serum CA-125 levels. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 25-year-old woman with left adnexal mass and extremely elevated serum CA-125 level, 7,900 U/mL, underwent laparoscopy. There were unilateral endometrioma...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.12.076
更新日期：2007-10-01 00:00:00
abstract：OBJECTIVE:To evaluate the effectiveness of growth hormone (GH) supplementation in improving the in vitro fertilization (IVF) outcomes of poor responders. DESIGN:Systematic review and meta-analysis. SETTING:Not applicable. PATIENT(S):Poor ovarian responders undergoing conventional IVF or intracytoplasmic sperm inject...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.03.007
更新日期：2020-07-01 00:00:00
