Morphologic and functional studies of immature rat oocyte-cumulus complexes after cryopreservation.


:The effects of freezing and thawing (F/T) on functional activity of immature rat oocyte-cumulus complexes (OCC) were studied. The OCC were divided into three groups according to the number of cumulus-cell layers surrounding them. The OCC were then frozen and thawed (F/T), with dimethyl sulfoxide (DMSO) as cryoprotectant. The survival rates after thawing increased significantly (P less than 0.001) as the number of cumulus-cell layers increased. Germinal vesicle breakdown (GVBD) was evaluated in F/T oocytes. After 2 hours, significantly (P less than 0.05) fewer oocytes demonstrated GVBD than did those in the control group. There was no difference, however, after 4 hours of culture. A significant (P less than 0.05) decrease in follicle-stimulating hormone (FSH)-dependent cyclic adenosine monophosphate (cAMP) accumulation was observed in the F/T group. However, the amount of cAMP produced was sufficient to maintain the oocyte in meiotic arrest. There was a borderline significant decrease of the coupling between cumulus cells and the oocyte in F/T OCC, as evaluated by the transport of 3H-uridine into the oocyte. It is concluded that immature rat oocytes can be successfully cryopreserved when they are surrounded by five or more layers of cumulus cells. FSH responsiveness and intercellular communication were essentially maintained. There was a slight delay in GVBD, which needs further clarification.


Fertil Steril


Fertility and sterility


Pellicer A,Lightman A,Parmer TG,Behrman HR,De Cherney AH


Has Abstract


1988-11-01 00:00:00














  • A clinical pregnancy after a simple method of zona cutting, cryopreservation, and zygote intrafallopian transfer.

    abstract::A 30-year-old nulliparous woman, who was infertile for greater than 3 years, conceived after the procedures of a simple method of zona cutting, cryopreservation, and ZIFT. This case illustrates: (1) acid medium, chymotrypsin, or sucrose are not needed for the procedure of zona cutting; (2) the zygotes resulting from z...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Chang SY,Soong YK,Chang MY,Lin PW,Guu HF,Wang ML

    更新日期:1991-02-01 00:00:00

  • Human cumulus cell gene expression as a biomarker of pregnancy outcome after single embryo transfer.

    abstract:OBJECTIVE:To identify the cumulus cell gene expression associated with oocyte developmental competence, specifically live birth, after single ET (SET) assisted reproductive technology. DESIGN:Retrospective gene expression analysis in human cumulus cells from oocytes that established a pregnancy resulting in live birth...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Gebhardt KM,Feil DK,Dunning KR,Lane M,Russell DL

    更新日期:2011-07-01 00:00:00

  • Influence of subfertility and assisted reproductive technology treatment on mortality of women after delivery.

    abstract:OBJECTIVE:To compare incidence, risk factors, and etiology of women's deaths in fertile, subfertile, and undergoing assisted reproductive technology (ART) in the years after delivery. DESIGN:Retrospective cohort. SETTING:University hospital. PATIENT(S):Women who had delivered in Massachusetts. INTERVENTION(S):This ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Coddington CC,Gopal D,Cui X,Cabral H,Diop H,Stern JE

    更新日期:2020-03-01 00:00:00

  • The use of transvaginal color flow imaging after in vitro fertilization to identify optimum uterine conditions before embryo transfer.

    abstract:OBJECTIVE:To assess whether a measure of uterine blood flow impedance (the pulsatility index, PI) as determined by transvaginal ultrasonography with color blood flow imaging, may be used to assess endometrial receptivity immediately before the time of embryo transfer (ET) after assisted conception. DESIGN:A prospectiv...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Steer CV,Campbell S,Tan SL,Crayford T,Mills C,Mason BA,Collins WP

    更新日期:1992-02-01 00:00:00

  • Insights from gene arrays on the development and growth regulation of uterine leiomyomata.

    abstract:OBJECTIVE:To use microarray analysis as an unbiased approach to identify genes involved in the induction and growth of uterine leiomyomata. DESIGN:Screen by arrays for up to 12,000 genes in leiomyoma (L) and control myometrium (M) from nine patients. SETTING:University research laboratories. PATIENT(S):Nine patients...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Tsibris JC,Segars J,Coppola D,Mane S,Wilbanks GD,O'Brien WF,Spellacy WN

    更新日期:2002-07-01 00:00:00

  • Studies of gene expression in human cumulus cells indicate pentraxin 3 as a possible marker for oocyte quality.

    abstract:OBJECTIVE:To search for differentially expressed genes in cumulus cells from two groups of oocytes with different developmental outcome in vitro. DESIGN:Analyses of gene expression in human cumulus cells from oocytes that failed to fertilize in vitro (group A) and those that developed into normal-appearing embryos on ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Zhang X,Jafari N,Barnes RB,Confino E,Milad M,Kazer RR

    更新日期:2005-04-01 00:00:00

  • Surgically induced ovulation in the polycystic ovary syndrome: wedge resection revisited in the age of laparoscopy.

    abstract:OBJECTIVE:To provide an up-to-date comprehensive review of published data on laparoscopic methods of ovulation induction in the polycystic ovary syndrome (PCOS). Areas to be considered include the historical background of these procedures, their outcomes as reported in the literature, and their potential adverse effect...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Donesky BW,Adashi EY

    更新日期:1995-03-01 00:00:00

  • Introduction: Uterus transplantation.

    abstract::Uterus transplantation is the first available treatment for absolute uterine factor infertility. Live births have been reported after transplantation of uteri both from live and deceased donors. Although this novel infertility treatment is still at its experimental stage, with human attempts performed within clinical ...

    journal_title:Fertility and sterility



    authors: Brännström M

    更新日期:2019-07-01 00:00:00

  • Follicular development in transplanted fetal and neonatal mouse ovaries is influenced by the gonadal status of the adult recipient.

    abstract:OBJECTIVE(S):To investigate the pattern of follicular development in transplanted fetal and neonatal mouse ovaries in the presence or absence of the recipient's own ovaries. DESIGN:Controlled experiment. SETTING:Academic research laboratory, Department of Physiology, Monash University, Clayton, Australia. INTERVENTI...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Cox S,Shaw J,Jenkin G

    更新日期:2000-08-01 00:00:00

  • Nulliparity, fertility treatments and twins: a time for rethinking.

    abstract:OBJECTIVE:To evaluate the risk that nulliparity poses to the outcome of twin pregnancies, an issue that gained importance due to the rise of twin gestations following assisted reproduction interventions. DESIGN:A prospective cohort study. SETTING AND PATIENT(S):Between January 1, 2004, and January 7, 2008, we prospec...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Berkovitz A,Hershko-Klement A,Fejgin M

    更新日期:2010-04-01 00:00:00

  • Correlation between serum antichlamydial antibodies and tubal factor as a cause of infertility.

    abstract::Although salpingitis frequently produces tubal damage and infertility, many women with tubal factor as a cause of their infertility do not have a clinical history of salpingitis. In order to investigate whether or not some such cases might be due to subclinical chlamydial infections, we measured antichlamydial antibod...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Jones RB,Ardery BR,Hui SL,Cleary RE

    更新日期:1982-11-01 00:00:00

  • Profiling signaling proteins in human spermatozoa: biomarker identification for sperm quality evaluation.

    abstract:OBJECTIVE:To determine the correlation between semen basic parameters and the expression and activity of signaling proteins. DESIGN:In vitro studies with human spermatozoa. SETTING:Academic research institute. PATIENT(S):Thirty-seven men provided semen samples for routine analysis. INTERVENTION(S):None. MAIN OUTCO...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Silva JV,Freitas MJ,Correia BR,Korrodi-Gregório L,Patrício A,Pelech S,Fardilha M

    更新日期:2015-10-01 00:00:00

  • The effect of egg yolk medium on human sperm binding in the hemizona assay.

    abstract:OBJECTIVE:To evaluate, using the hemizona assay (HZA), whether egg yolk treatment of human sperm enhances binding to the human zona pellucida in vitro and to determine whether such a treatment is as efficient as the standard swim-up procedure for promoting sperm binding ability. DESIGN:Ejaculates were divided into ali...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Lanzendorf SE,Holmgren WJ,Jeyendran RS

    更新日期:1992-09-01 00:00:00

  • Significance of one pronucleus before fertilization.

    abstract:OBJECTIVE:To describe a case of primary infertility associated with oocytes having one pronucleus before fertilization on repeated IVF attempts. DESIGN:Case report. SETTING:A university-based assisted reproduction unit. PATIENT(S):A 30-year-old woman with primary infertility and oocytes containing one pronucleus bef...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Westphal LM,Rosencrantz M,Behr B,Milki AA

    更新日期:2003-04-01 00:00:00

  • Sperm penetration into cervical mucus in vitro. I. Comparative studies.

    abstract::Comparative studies have been carried out on the behavior of human and bovine spermatozoa toward homologous cervical mucus in vitro. In both cases the degree of sperm penetration and the pattern of sperm motility were influenced in a characteristic fashion by prior manipulation of the mucus: the most rapid and extensi...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Gaddum-Rosse P,Blandau RJ,Lee WI

    更新日期:1980-06-01 00:00:00

  • Outcome of ovarian stimulation for oocyte cryopreservation in women with Turner syndrome.

    abstract:OBJECTIVE:To study the safety and efficacy of ovarian stimulation and oocyte cryopreservation as a method of fertility preservation in women with Turner syndrome (TS). DESIGN:Retrospective cohort study. SETTING:Reproductive medicine clinic. PATIENT(S):Seven women with TS who attended the clinic between 2011 and 2017...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Talaulikar VS,Conway GS,Pimblett A,Davies MC

    更新日期:2019-03-01 00:00:00

  • Embryo transfer technique as a cause of ectopic pregnancies in in vitro fertilization.

    abstract::Against an overall risk of around 2% ectopic pregnancies in in vitro fertilization programs around the world, we report an incidence more than three times greater (5 tubal ectopic pregnancies in 80 clinical in vitro fertilization pregnancies). Of two techniques used for embryo transfers, one produced a significantly h...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Yovich JL,Turner SR,Murphy AJ

    更新日期:1985-09-01 00:00:00

  • Interruption of pregnancy by prostaglandin 15-methyl F2alpha.

    abstract::The current study was formulated to investigate the abortifacient activity of prostaglandin 15-methyl F2alpha (15-methyl PGF2alpha) administered intramuscularly to 80 healthy women with gestations between 8 and 22 weeks. Goals were the establishment of an effective dosage schedule and assessment of the incidence and s...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Bolognese RJ,Corson SL

    更新日期:1975-07-01 00:00:00

  • The use of lead follicle diameter to initiate gonadotropin-releasing hormone antagonist does not affect in vitro fertilization clinical pregnancy, implantation, or live birth rates: a prospective, randomized study.

    abstract::In this prospective, randomized study, waiting for the lead follicle to reach 14 mm before initiating GnRH antagonist administration effectively prevents an LH surge and ovulation during an IVF cycle. Furthermore, delaying GnRH start until the dominant follicle reaches 14 mm neither impacts the clinical pregnancy, imp...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,随机对照试验


    authors: Dayal MB,Frankfurter D,O'Hern C,Peak D,Dubey A,Gindoff PR

    更新日期:2009-12-01 00:00:00

  • Evidence for a histaminergic system in the human testis.

    abstract::The complete lack of information about mast cells as a source of histamine and potential target cells for histamine in human testes prompted us to investigate these issues in testes of fertile and infertile patients using a combination of laser microdissection, reverse transcription-polymerase chain reaction (RT-PCR),...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Albrecht M,Frungieri MB,Gonzalez-Calvar S,Meineke V,Köhn FM,Mayerhofer A

    更新日期:2005-04-01 00:00:00

  • Risk of type 2 diabetes is increased in nonobese women with polycystic ovary syndrome: the National Health Insurance Service-National Sample Cohort Study.

    abstract:OBJECTIVE:To investigate the relationship between polycystic ovary syndrome (PCOS) and type 2 diabetes mellitus (T2DM) in Korean women. DESIGN:Longitudinal case-control study. SETTING:Not applicable. PATIENT(S):PCOS patients aged 15 to 44 years (n = 1,136) and control individuals (n = 5,675), matched 1:5 by age grou...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Ryu KJ,Kim MS,Kim HK,Kim YJ,Yi KW,Shin JH,Hur JY,Kim T,Park H

    更新日期:2021-01-25 00:00:00

  • Changes of bioactive luteinizing hormone after laparoscopic ovarian cautery in patients with polycystic ovarian syndrome.

    abstract::The serum levels of bioactive luteinizing hormone (LH), immunoreactive LH, follicle-stimulating hormone, androstenedione (A), and testosterone (T) were determined in nine anovulatory women with polycystic ovarian syndrome (PCOS) before and after laparoscopic ovarian cautery. Eight ovulated spontaneously and three conc...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Sakata M,Tasaka K,Kurachi H,Terakawa N,Miyake A,Tanizawa O

    更新日期:1990-04-01 00:00:00

  • Role of follicle-stimulating hormone receptor Ser680Asn polymorphism in the efficacy of follicle-stimulating hormone.

    abstract:OBJECTIVE:To evaluate the association between FSH efficacy and FSHR alleles. DESIGN:Retrospective study. SETTING:University-based fertility unit and a private center for biomedical research. PATIENT(S):One hundred two women with ovarian function who were undergoing controlled ovarian stimulation (COS). Women were ca...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: de Castro F,Ruiz R,Montoro L,Pérez-Hernández D,Sánchez-Casas Padilla E,Real LM,Ruiz A

    更新日期:2003-09-01 00:00:00

  • Lymphatic spread of endometriosis to pelvic sentinel lymph nodes: a prospective clinical study.

    abstract:OBJECTIVE:To establish the prevalence of endometriosis metastatic to pelvic sentinel lymph nodes (PSLN) in women with ovarian and/or peritoneal endometriosis. DESIGN:Prospective clinical study. SETTING:Academic research institution. PATIENT(S):Women with a laparoscopic diagnosis of ovarian and/or peritoneal endometr...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Tempfer CB,Wenzl R,Horvat R,Grimm C,Polterauer S,Buerkle B,Reinthaller A,Huber JC

    更新日期:2011-09-01 00:00:00

  • Spontaneous conception after a successful attempt at in vitro fertilization/intracytoplasmic sperm injection.

    abstract:OBJECTIVE:To determine the incidence of spontaneous pregnancy in women who were not actively undergoing therapy after a successful attempt at IVF/intracytoplasmic sperm injection and to characterize its pattern of occurrence. DESIGN:Retrospective postal questionnaire. SETTING:An assisted reproduction unit at a univer...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Hennelly B,Harrison RF,Kelly J,Jacob S,Barrett T

    更新日期:2000-04-01 00:00:00

  • Female obesity increases the risk of miscarriage of euploid embryos.

    abstract:OBJECTIVE:To determine whether female body mass index (BMI) is associated with an increased risk of miscarriage after euploid embryo transfer. DESIGN:A retrospective, observational, multicenter cohort study. SETTING:University-affiliated in vitro fertilization center. PATIENT(S):In this study, 3,480 cycles of in vit...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Cozzolino M,García-Velasco JA,Meseguer M,Pellicer A,Bellver J

    更新日期:2020-11-30 00:00:00

  • Are pregnancy planning and timing associated with preterm or small for gestational age births?

    abstract:OBJECTIVE:To investigate whether unplanned or poorly timed pregnancies (self-reported at enrollment) are associated with preterm or small for gestational age births. DESIGN:Prospective cohort study. SETTING:Not applicable. PATIENT(S):Two thousand six hundred fifty-four pregnant women <18 weeks estimated gestational ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,多中心研究


    authors: Gariepy AM,Lundsberg LS,Stolar M,Stanwood NL,Yonkers KA

    更新日期:2015-12-01 00:00:00

  • Risk factors for extrauterine pregnancy in women using an intrauterine device.

    abstract:OBJECTIVE:To identify the risk factors for ectopic pregnancy (EP) in women using an intrauterine device (IUD). DESIGN:Case-control study. SETTING:Auvergne region (France). PATIENT(S):Women using an IUD and suffering EP (243 cases) or having an intrauterine pregnancy (140 controls). INTERVENTION(S):None. MAIN OUTCO...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Bouyer J,Rachou E,Germain E,Fernandez H,Coste J,Pouly JL,Job-Spira N

    更新日期:2000-11-01 00:00:00

  • Measurement of the ovarian follicle by ultrasound in ovulation induction.

    abstract::Ultrasonic monitoring of ovarian follicles and estimation of serum estradiol were carried out in 12 patients with clomiphene therapy, in 5 patients with gonadotropin therapy, and in 7 normal controls. The average diameter of preovulatory follicles in normal controls was 12,8 mm; in ovulation induction groups it was 2 ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Ylöstalo P,Rönnberg L,Jouppila P

    更新日期:1979-06-01 00:00:00

  • Relation between indices of semen analysis and pregnancy rate in infertile couples.

    abstract::Both partners of 198 consecutive infertile couples were evaluated. Studies in the female included clinical evaluation of ovulation for a minimum of two menstrual cycles, investigation of tubal patency, and evaluation of sperm survival in cervical mucus. Six semen specimens were analyzed from each male. Laparoscopy, sc...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Smith KD,Rodriguez-Rigau LJ,Steinberger E

    更新日期:1977-12-01 00:00:00