Abstract：
OBJECTIVE:To determine the expression profiles of the DAZ gene family in men with and without spermatogenic failure. DESIGN:Prospective case study. SETTING:University-based reproductive clinics and genetics laboratory. PATIENT(S):Thirty-four infertile men presenting with azoospermia. INTERVENTION(S):The mRNA transcript concentrations of the DAZ family genes (BOULE, DAZL, DAZ) and the housekeeping GAPDH gene in the testes of azoospermic men were examined by quantitative competitive-reverse transcription-polymerase chain reaction (QC-RT-PCR). The steady-state concentrations of mRNA encoding for each gene in each testicular sample were normalized by the amounts of GAPDH. MAIN OUTCOME MEASURE(S):Transcript ratios (gene/GAPDH) of BOULE, DAZL, and DAZ. RESULT(S):The transcript ratios for BOULE, DAZL, and DAZ were significantly decreased in tissues with spermatogenic failure (hypospermatogenesis, maturation arrest, and Sertoli cell-only). However, the ratios of BOULE/DAZL and DAZ/DAZL did not reveal any significant difference in all tissues. Three patients with DAZ deletion possess lower transcripts of BOULE and DAZL. CONCLUSION(S):All members of the DAZ gene family play important roles in human spermatogenesis. Decreased concentrations of DAZ family members in men with spermatogenic failure may be due to the secondary effect of germ cell loss, and transcriptional control of BOULE, DAZL, and DAZ are not altered in the various degrees of spermatogenic failure. Although the sample size is limited, no compensatory increase of DAZL or BOULE transcription was found in men with DAZ deletion.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Kuo PL,Wang ST,Lin YM,Lin YH,Teng YN,Hsu CCdoi
10.1016/j.fertnstert.2003.08.047keywords:
subject
Has Abstractpub_date
2004-04-01 00:00:00pages
1034-40issue
4eissn
0015-0282issn
1556-5653pii
S0015028203030917journal_volume
81pub_type
杂志文章abstract：OBJECTIVE:To compare the efficacy of two antiandrogens, spironolactone and flutamide, in the treatment of hirsutism. PATIENTS, PARTICIPANTS:Twenty women with idiopathic hirsutism were randomized to receive either flutamide or spironolactone. DESIGN:Twenty hirsute women were recruited from patients presenting to the h...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1994-04-01 00:00:00
abstract：OBJECTIVE:To determine the role of heterozygosity for mutations in the 21-hydroxylase gene (CYP21) in the pathogenesis of hyperandrogenism. DESIGN:Controlled clinical study. SETTING:Tertiary care institutional hospital. PATIENT(S):Forty hirsute women and 13 healthy control women. INTERVENTION(S):The source of andro...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(99)00317-9
更新日期：1999-10-01 00:00:00
abstract：:Hypoxia downregulated the concentration of endostatin in the culture media of human endometrial stromal cells but did not affect the messenger (m)RNA expression of collagen XVIII. Both mRNA and protein expression of vascular endothelial growth factor were upregulated in a hypoxic condition. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.03.032
更新日期：2004-09-01 00:00:00
abstract：OBJECTIVE:To evaluate sperm DNA fragmentation in normozoospermic male partners of couples undergoing infertility evaluation. DESIGN:Retrospective cohort study. SETTING:Clinical andrology laboratory. PATIENT(S):A total of 1,974 consecutive normozoospermic men selected from a larger cohort of 4,345 consecutive, nonazo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.02.006
更新日期：2014-06-01 00:00:00
abstract：:Forty years ago this month the first baby was born as a result of in vitro fertilization (IVF). The arrival of Louise Brown brought with her a new hope and possibility for the many men and women across the global struggling with infertility who previously had very few options for treatment or family building. In this ...
journal_title：Fertility and sterility
pub_type： 历史文章
doi：10.1016/j.fertnstert.2018.05.017
更新日期：2018-07-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to assess which of the basal ovarian reserve markers provides the best reflection of the changes occurring in ovarian function over time (i.e., reproductive aging). DESIGN:Prospective longitudinal study. SETTING:Healthy volunteers in an academic research center. PATIENT(S):Eighty-...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.11.029
更新日期：2005-04-01 00:00:00
abstract：OBJECTIVE:To evaluate whether the use of theophylline improves sperm motility and treatment outcome in frozen-thawed testicular sperm extraction (TESE). DESIGN:Artificial sperm activation was offered to azoospermic patients between January and October 2010 in two different centers (identical lab conditions). SETTING:...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.08.041
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVE:To provide an up-to-date comprehensive review of published data on laparoscopic methods of ovulation induction in the polycystic ovary syndrome (PCOS). Areas to be considered include the historical background of these procedures, their outcomes as reported in the literature, and their potential adverse effect...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/s0015-0282(16)57408-1
更新日期：1995-03-01 00:00:00
abstract：:Semen specimens were obtained for analysis from nine men immediately before vasectomy and 3 days following the vasectomy procedure. After vasectomy there was a significant decrease in seminal sperm concentration, percentage of sperm motility, and mean swimming speed. In seven of nine cases the spermatozoa did not surv...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)48262-2
更新日期：1984-12-01 00:00:00
abstract：OBJECTIVE:To determine whether luteal phase defect (LPD) may be an etiologic factor in ectopic pregnancy (EP). DESIGN:All patients who were seen over a 6-year period with the chief complaint of infertility underwent an extensive infertility workup and were followed prospectively. The diagnoses of the causes of inferti...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)57292-6
更新日期：1995-01-01 00:00:00
abstract：OBJECTIVE:To determine inhibin-B concentrations during ovarian reserve screening in women with normal and diminished ovarian reserve as determined by the clomiphene citrate challenge test. DESIGN:Retrospective. SETTING:Tertiary fertility center. PATIENT(S):Women undergoing ovarian reserve screening for a routine fer...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)00531-1
更新日期：1998-03-01 00:00:00
abstract：OBJECTIVE:To report the results of 20 orthotopic retransplantations of cryopreserved ovarian tissue after cancer treatment. DESIGN:Retrospective analysis. SETTING:Tertiary gynecology department. PATIENT(S):Twenty patients with malignant disease: 11 with hematological malignancies (55%), four with breast cancer (20%)...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.10.045
更新日期：2015-02-01 00:00:00
abstract：OBJECTIVE:To determine the prevalence of anticardiolipin and antiphophatidylserine antibodies in an IVF population and to correlate their presence and specific isotype with IVF cycle outcome. DESIGN:Retrospective clinical study using stored midfollicular sera for determination of antibody status. SETTING:University h...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)81519-1
更新日期：1997-08-01 00:00:00
abstract：OBJECTIVE:To examine gonadal protective properties of granulocyte colony-stimulating factor (G-CSF) alone or in combination with stem cell factor (SCF) in female mice treated with high-dose alkylating chemotherapy. DESIGN:Experimental laboratory animal study. SETTING:Tertiary care academic hospital and research insti...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.01.135
更新日期：2013-06-01 00:00:00
abstract：:It is suggested that heparin given in the peri-implantation period may improve clinical outcomes in women undergoing assisted reproduction techniques (ART). This systematic review evaluates the use of heparin in subfertile women undergoing ART. ...
journal_title：Fertility and sterility
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.fertnstert.2014.09.005
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVE:To investigate whether the Ala16Val polymorphism in the SOD2 gene, encoding for mitochondrial manganese superoxide dismutase (SOD), is associated with [1] infertility and [2] the pregnancy rate (PR) in IVF cycles. DESIGN:Prospective case-control study. SETTING:Public university and public university hospita...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2010.11.022
更新日期：2011-04-01 00:00:00
abstract：:A prospective randomized study was conducted in 100 women with leiomyomas in order to evaluate the effect of gestrinone, a synthetic derivative of ethynil-nor-testosterone. Patients in group A received capsules containing 2.5 mg of gestrinone three times weekly orally. Those in group B received capsules containing 5.0...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)60722-7
更新日期：1989-06-01 00:00:00
abstract：:This document is designed to assist in vitro fertility clinics in the management of cryopreserved reproductive tissues stored in cryogenic storage (cryostorage) tanks, based upon scientific principles and laboratory experience related to best practice for safe and reliable storage of cryopreserved reproductive tissue....
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.06.019
更新日期：2020-09-01 00:00:00
abstract：:A simple, sensitive, and reliable single-unit nonradioactive method for the detection of human chorionic gonadotropin (hCG) in concentrated urine and the diagnosis of early pregnancy is reported. This unit, presently termed the Ayerst pregnancy test kit (APTK), consists of four components: a sampler-filter paper cone,...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44299-8
更新日期：1979-10-01 00:00:00
abstract：OBJECTIVE:To determine whether nerve fibers can be detected in the endometrium and myometrium in women with painful uterine fibroids and adenomyosis. DESIGN:A retrospective immunohistochemical study. SETTING:An academic training hospital. PATIENT(S):Thirty-seven women with uterine fibroids and 29 women with adenomyo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.03.026
更新日期：2010-07-01 00:00:00
abstract：OBJECTIVE:To examine the association between the midfollicular FSH-LH ratio and the number of follicles, and the multifollicular ovarian response to gonadotropin stimulation in patients with polycystic ovary syndrome (PCOS) with normal basal LH and FSH levels. SUBJECT(S):Eighteen patients who had an abandoned treatmen...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)80069-6
更新日期：1997-03-01 00:00:00
abstract：:The Ethics Committee recommends development of evidence-based policies that are patient centered for each IVF center. In most cases, the provision of futile therapies is not ethically justifiable. For those treatments with very poor success rates, clinicians must be vigilant in their presentation of risks, benefits, a...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2009.07.979
更新日期：2009-10-01 00:00:00
abstract：OBJECTIVE:To study the effects of peritoneal macrophages on endometrial cellular proliferation in an in vitro coculture model and to compare the magnitude of these effects between macrophages from women with endometriosis and normal women. DESIGN:Controlled study of peritoneal macrophage function. SETTING:University ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00292-7
更新日期：1999-09-01 00:00:00
abstract：OBJECTIVE:To investigate, with pre-COVID-19 data, whether parental exposure to severe systemic infections near the time of conception is associated with pregnancy outcomes. DESIGN:Retrospective cohort study. SETTING:Population-based study covering births within the United States from 2009 to 2016. PARTICIPANTS:The I...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.09.153
更新日期：2020-12-01 00:00:00
abstract：OBJECTIVE:To study pain in women undergoing oocyte retrieval with a reduced needle (20/17 gauge) compared to a standard needle (16 gauge). DESIGN:Single-center randomized controlled trial. SETTING:Fertility clinic. PATIENTS:Women undergoing their first oocyte retrieval for in vitro fertilization or intracytoplasmic ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2020.06.040
更新日期：2020-10-16 00:00:00
abstract：:A battery-powered, bipolar electrocoagulator has been specifically developed for sealing the cut ends of the divided vas at vasectomy. With a minimum of electric power, the electrocoagulator destroys only the mucosa and one or two muscle cell layers of the vas, which leads to optimal fibrosis of the cut ends. This ins...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1978-06-01 00:00:00
abstract：OBJECTIVE:To study attitudes about posthumous assisted reproduction (PAR) in individuals presenting for assisted reproduction, to examine the degree of concordance of attitudes within couples, and to determine whether individuals can accurately predict the attitudes of their intimate partners. DESIGN:Cross sectional s...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2011.09.018
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVE:To examine whether a relationship exists between genetic polymorphisms of glutathione S-transferase (GST) M1 and T1, CYP1A1(*)2C, and male factor infertility. DESIGN:Genetic polymorphism analysis, case-control study. SETTING:University research laboratory and andrology clinic. PATIENT(S):One hundred ten me...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.07.017
更新日期：2009-08-01 00:00:00
abstract：OBJECTIVE:To examine the effects of coping and of infertility-specific and nonspecific stress on pregnancy outcomes after IVF treatment. DESIGN:Cross-sectional design controlling for potential confounding variables. Psychosocial measures were assessed on the day of embryo transfer. SETTING:Fertility clinic in Greece....
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.02.096
更新日期：2006-09-01 00:00:00
abstract：:Seminiferous tubules from rats killed 24 hours after injection of pargyline, a monoamine oxidase inhibitor, did not grow well in tissue culture when compared to control tubules. Treated tubules showed severe tubular degeneration and loss of cellular detail after nine days in culture. Animals injected with pargyline fo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)40992-1
更新日期：1975-03-01 00:00:00
