Abstract：
OBJECTIVE:To evaluate, using the hemizona assay (HZA), whether egg yolk treatment of human sperm enhances binding to the human zona pellucida in vitro and to determine whether such a treatment is as efficient as the standard swim-up procedure for promoting sperm binding ability. DESIGN:Ejaculates were divided into aliquots and half incubated at 37 degrees C for 21 hours in standard culture medium or combined with buffered medium containing chicken egg yolk and stored at 4 degrees C for 21 hours. A second set of ejaculates were split and processed by a standard 1-hour rise alone or treated with egg yolk medium. SETTING:University teaching hospital. PARTICIPANTS:Two healthy sperm donors. MAIN OUTCOME MEASURE:The number of sperm tightly bound to the hemizona were measured and compared between the groups. RESULTS:A significant increase (P less than 0.0001) in hemizona binding (n = 46) for egg yolk treatment (90.1 +/- 9.8; range 7 to 258) as compared with standard culture medium (53.0 +/- 8.8; range 0 to 228) was observed. Similarly, a significant increase (P less than 0.0001) in binding (n = 37) for egg yolk treatment (74.9 +/- 8.2; range 7 to 219) as compared with samples obtained with a sperm rise (37.1 +/- 5.7; range 2 to 122). CONCLUSIONS:Treatment of human spermatozoa with an egg yolk medium at 4 degrees C overnight significantly increases sperm binding in the HZA.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Lanzendorf SE,Holmgren WJ,Jeyendran RSdoi
10.1016/s0015-0282(16)55261-3keywords:
subject
Has Abstractpub_date
1992-09-01 00:00:00pages
547-50issue
3eissn
0015-0282issn
1556-5653pii
S0015-0282(16)55261-3journal_volume
58pub_type
杂志文章abstract：OBJECTIVE:To investigate the slope of linear regression of postevacuation serum hCG as an independent risk factor for postmolar gestational trophoblastic neoplasia (GTN). DESIGN:Multicenter retrospective cohort study. SETTING:Academic referral health care centers. PATIENT(S):All subjects with confirmed hydatidiform ...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究
doi：10.1016/j.fertnstert.2015.06.001
更新日期：2015-09-01 00:00:00
abstract：OBJECTIVE:To determine whether fallopian sperm perfusion significantly improves pregnancy rates over intrauterine insemination (IUI) in infertile patients. DESIGN:Prospective, randomized, controlled trial and metaanalysis of the literature. SETTING:University-based infertility practice. PATIENT(S):Patients undergoin...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,meta分析,随机对照试验
doi：10.1016/s0015-0282(99)00068-0
更新日期：1999-05-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of estrogen and progestogen on the resistance to blood flow in the uterine arteries of Turner's syndrome patients. DESIGN:Prospective clinical study. SETTING:A tertiary infertility clinic. PATIENTS:Five Turner's syndrome patients, six patients who had surgical castration, and five...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)57968-0
更新日期：1995-12-01 00:00:00
abstract：OBJECTIVE:To determine whether the known inactivating FSH receptor gene mutations are present in Japanese women with secondary amenorrhea because of premature ovarian failure (POF) and polycystic ovary syndrome (PCOS). DESIGN:Clinical and molecular studies. SETTING:An outpatient clinic in a university hospital. PATI...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(00)01673-3
更新日期：2001-01-01 00:00:00
abstract：:Fifteen subfertile males with absent or atrophic right testes underwent ligation of left varicoceles. Sperm concentration rose significantly with correction of scrotal thermographic abnormality and nine wives (60%) became pregnant. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)55098-5
更新日期：1992-06-01 00:00:00
abstract：OBJECTIVE:To investigate the direct effects of sodium nitroprusside, a nitric oxide donor, on the fertilizing ability of human spermatozoa in vitro. DESIGN:Prospective, controlled in vitro study. SETTING:IVF Unit, Medical College Hospital. PATIENT(S):Women undergoing conventional IVF. INTERVENTION(S):Human spermato...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)00537-2
更新日期：1998-03-01 00:00:00
abstract：:In this observational, case-control study in infertile women, we observed elevated serum oxidative stress that was significantly correlated with an increase in serum paraoxonase-1 activity and without any evidence of a proinflammatory reaction. These results suggest a protective role of paraoxonase-1 against inflammat...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.11.043
更新日期：2010-08-01 00:00:00
abstract：OBJECTIVE:To measure the effect of competition among fertility centers on pregnancy and high-order multiple (HOM) gestation rates after IVF. DESIGN:Retrospective cohort study. SETTING:Four hundred eight fertility clinics registered with the Society for Assisted Reproductive Technology as providing IVF services in 200...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.10.048
更新日期：2005-05-01 00:00:00
abstract：OBJECTIVE:To evaluate the efficacy of blastocyst culture and transfer in human in vitro fertilization (IVF) as compared to day 3 embryo transfer. DESIGN:Prospective randomized trial. SETTING:Private assisted reproduction unit. PATIENT(S):A total of 162 IVF patients were included in the day 3 embryo transfer (n = 82)...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(01)02939-9
更新日期：2002-01-01 00:00:00
abstract：:Twelve adult does in estrus were artificially inseminated with 1 ml of semen from bucks that had received injections of 3H-thymidine 22 to 32 days previously. The ejaculates contained radioactive seminal plasma and unlabeled sperm. The reproductive tracts of the does were removed at selected intervals after inseminati...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42622-1
更新日期：1977-06-01 00:00:00
abstract：OBJECTIVE:To evaluate the association between leiomyomas and estrogen receptor gene polymorphism. DESIGN:Prospective study. SETTING:Department of gynecology and genetics. PATIENT(S):Women with (n = 159) or without leiomyomas (n = 131). MAIN OUTCOME MEASURE(S):Polymerase chain reaction was used to detect dinucleotid...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)04563-6
更新日期：2003-01-01 00:00:00
abstract：OBJECTIVE:To determine the proportion of infertile women who prefer a multiple birth over a singleton, patient characteristics associated with this desire, and patient knowledge about the risks of multiple births. DESIGN:Prospective analysis. SETTING:Academic university hospital-based infertility center and private g...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2003.05.035
更新日期：2004-03-01 00:00:00
abstract：OBJECTIVE:To investigate whether cigarette smoking affects the blood lead levels (BLL) and whether exposure to lead introduces sperm chromatin DNA damage in factory workers. DESIGN:Cross-sectional study. SETTING:A battery plant in Taiwan. PATIENT(S):Eighty male workers employed within a battery plant. INTERVENTION(...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.01.005
更新日期：2009-04-01 00:00:00
abstract：OBJECTIVE:To investigate whether nicotine-induced testicular toxicity involves the induction of apoptosis in Leydig cells. DESIGN:Cell study using cells of the TM3 cell line derived from mouse Leydig cells. SETTING:Academic research laboratory. INTERVENTION(S):Morphological and biochemical analyses for the detection...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.12.013
更新日期：2005-04-01 00:00:00
abstract：:In 6-day pregnant New Zealand White rabbits dosed intravenously with 3H-nicotine, the 3H-activity in the uterine fluid was approximately 5 to 11 times greater than that in the plasma at the corresponding times; 3H-nicotine itself accounted for most of this radioactivity. Dichlorodiphenyltrichloroethane (DDT) also accu...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1976-10-01 00:00:00
abstract：OBJECTIVE:To review current knowledge regarding recombinant DNA technology and its safety and efficacy in relation to recombinant gonadotropin production. DATA IDENTIFICATION AND SELECTION:Studies that relate specifically to recombinant DNA technology, method of laboratory production, and the clinical aspects of using...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/s0015-0282(16)58437-4
更新日期：1996-08-01 00:00:00
abstract：:We describe a cotyledonoid dissecting leiomyoma in a 33-year-old nulliparous woman treated by laparoscopy. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.08.131
更新日期：2009-04-01 00:00:00
abstract：OBJECTIVE:Proinflammatory cytokines have been described to be involved in the pathogenesis of idiopathic recurrent miscarriage (IRM). We investigated the association between IRM and a polymorphism in exon 5 of the interleukin-1beta gene (IL1B) and interleukin-1beta (IL-1beta) serum levels. DESIGN:Case control study. ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(01)01914-8
更新日期：2001-08-01 00:00:00
abstract：OBJECTIVE:To introduce an effective approach for excision of ovarian endometrioma by "water injection"-assisted laparoscopy treatment. DESIGN:Step-by-step video explanation of the surgical procedure with still pictures and surgical video clips to demonstrate the detailed technique, approved by the Shengjing Hospital o...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2019.05.014
更新日期：2019-09-01 00:00:00
abstract：OBJECTIVE:To determine whether the G1733A polymorphism of the androgen receptor gene is associated with an increased risk for recurrent spontaneous abortion (RSA). DESIGN:Case-control study. SETTING:Division of Genetics and Biotechnology, Department of Biology, University of Athens. PATIENT(S):A total of 131 women w...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.04.071
更新日期：2008-11-01 00:00:00
abstract：OBJECTIVE:To assess the effectiveness of luteal start vaginal micronized P in a recurrent pregnancy loss (RPL) cohort. DESIGN:Observational cohort study using prospectively collected data. SETTING:Not applicable. PATIENT(S):Women seen between 2004 and 2012 with a history of two or more unexplained pregnancy losses <...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2016.11.029
更新日期：2017-03-01 00:00:00
abstract：OBJECTIVE:To determine whether NR5A1 (SF-1) variants are a cause of primary ovarian insufficiency (POI) in 26 young women with similar genetic background. DESIGN:Genetic and functional mutation study. SETTING:University hospitals. PATIENT(S):Genetic analysis of the NR5A1 gene in 26 XX girls with POI. INTERVENTION(S...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2012.10.026
更新日期：2013-02-01 00:00:00
abstract：OBJECTIVE:To determine the level of standardization in performance of the semen analysis among clinical laboratories in the United States. DESIGN:A survey was mailed to laboratories requesting information about the laboratory and performance of the semen analysis. Responses were received from 536 laboratories. SETTIN...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)03296-x
更新日期：2002-09-01 00:00:00
abstract：OBJECTIVE:To describe a patient with uterine rupture in the subsequent pregnancy after hysteroscopic resection of a uterine septum. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 30-year-old nulliparous woman, with a history of a first trimester spontaneous abortion, hysteroscopic uterine septum resec...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.08.075
更新日期：2009-03-01 00:00:00
abstract：:The hormonal profile of the aging male reveals an associated decrease in free testosterone and 5 alpha-dihydrotestosterone (DHT) levels and increased luteinizing hormone levels. Later events consist of a decrease in total testosterone, stable DHT, and increased follicle-stimulating hormone and estradiol levels. Althou...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)41958-8
更新日期：1976-07-01 00:00:00
abstract：:Suppressor activity in in vitro fertilization (IVF) culture medium correlates with successful implantation. High performance liquid chromatography fractionation revealed peak(s) of inhibitory activity in the 1,000- to 5,000-Da molecular weight range. Inhibitory activity was dependent on the presence of fetal bovine se...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1990-05-01 00:00:00
abstract：OBJECTIVE:To characterize and contrast protamine levels, chromatin decondensation, chromosome aneuploidy rates, and functional ability of round-headed sperm from two brothers with round-headed sperm syndrome. DESIGN:Analysis of semen samples from siblings with round-headed sperm syndrome and comparison with semen of f...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00498-1
更新日期：1999-03-01 00:00:00
abstract：:During hysteroscopy the uterus may be distended with carbon dioxide (CO2), nitrous oxide (N2O), or Hyskon (a high molecular weight dextran). An initial study in 27 patients (group 1) using arterialized venous blood samples demonstrated rises in carbon dioxide tension (PCO2) when N2O was insufflated by using a laparosc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44179-8
更新日期：1979-08-01 00:00:00
abstract：OBJECTIVE:To examine the effects of soy phytoestrogens on coronary vascular reactivity in atherosclerotic male and female rhesus monkeys. DESIGN:A prospective, randomized, blinded, controlled study. SETTING:Comparative Medicine Clinical Research Center of an academic medical center. PATIENT(S):Twenty-two young adult...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)81872-9
更新日期：1997-01-01 00:00:00
abstract：:The importance of the operating microscope in rabbit tubal anastomosis was assessed by operating on 36 rabbits, half with the aid of the operating microscope and half with the aid of low-magnification loupes. The surgeons' operating comfort and sense of assuredness that the anastomoses were being done properly were in...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)47303-6
更新日期：1983-09-01 00:00:00
