Effect of different procedures of semen preparation on antibody-coated spermatozoa and immunological infertility.

Abstract:

:To assess whether procedures of semen preparation can reduce the proportion of antibody-coated spermatozoa, semen samples with positive direct mixed antiglobulin reaction (MAR) were washed in media supplemented with 10% or 50% fetal cord serum (FCS). Washing reduced the MAR to a negative level, but the MAR was identical to that in the native semen when spermatozoa were resuspended in serum-free medium. Donor spermatozoa, recovered after swim-up in media supplemented with 10% or 50% FCS or after passage through a column with 7.5% human serum albumin (HSA), were incubated in serum samples with both agglutinating and cytotoxic antisperm antibodies. Cytotoxic activity was significantly reduced against sperm filtered over the albumin column.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Hinting A,Vermeulen L,Goethals I,Dhont M,Comhaire F

doi

10.1016/s0015-0282(16)53169-0

subject

Has Abstract

pub_date

1989-12-01 00:00:00

pages

1022-6

issue

6

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(16)53169-0

journal_volume

52

pub_type

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    更新日期:2007-10-01 00:00:00

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