Comparison of two colloidal silica-based sperm separation media with a non-silica-based medium.

Abstract:

OBJECTIVE:To study and compare the effects of three sperm separation media, two silica-based (Percoll; Pharmacia Biotech AB, Uppsala, Sweden, and Isolate; Irvine Scientific, Santa Ana, CA) and one non-silica-based (Ixaprep; Medicult, Copenhagen, Denmark), on the recovery of progressive motile sperm, the percentage of sperm with normal morphology, various sperm motion characteristics determined by computer-aided sperm analysis, and the percentage of acrosome-reacted sperm. DESIGN:Prospective study. SETTING:A university-based assisted reproductive technology center. PATIENT(S):Male partners of couples attending our infertility clinic. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Various semen parameters. RESULT(S):Both Isolate and Ixaprep resulted in enhanced recovery of motile spermatozoa compared with Percoll. The percentage of sperm with forward progressive motility, the percentage of sperm with normal morphology, and various sperm motion characteristics were similar after the use of Percoll and Isolate and were significantly better than after the use of Ixaprep. The same percentage of acrosome-reacted spermatozoa was observed with all three media. Similar results were observed in both normal and subnormal semen samples. CONCLUSION(S):The use of Isolate and Ixaprep resulted in better recovery of motile spermatozoa. Other semen parameters were similar with the use of Isolate and Percoll, whereas the use of Ixaprep was associated with lower sperm velocities and fewer morphologically normal spermatozoa.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Makkar G,Ng HY,Yeung SB,Ho PC

doi

10.1016/s0015-0282(99)00375-1

keywords:

subject

Has Abstract

pub_date

1999-11-01 00:00:00

pages

796-802

issue

5

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(99)00375-1

journal_volume

72

pub_type

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