Abstract:
OBJECTIVE:To study and compare the effects of three sperm separation media, two silica-based (Percoll; Pharmacia Biotech AB, Uppsala, Sweden, and Isolate; Irvine Scientific, Santa Ana, CA) and one non-silica-based (Ixaprep; Medicult, Copenhagen, Denmark), on the recovery of progressive motile sperm, the percentage of sperm with normal morphology, various sperm motion characteristics determined by computer-aided sperm analysis, and the percentage of acrosome-reacted sperm. DESIGN:Prospective study. SETTING:A university-based assisted reproductive technology center. PATIENT(S):Male partners of couples attending our infertility clinic. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Various semen parameters. RESULT(S):Both Isolate and Ixaprep resulted in enhanced recovery of motile spermatozoa compared with Percoll. The percentage of sperm with forward progressive motility, the percentage of sperm with normal morphology, and various sperm motion characteristics were similar after the use of Percoll and Isolate and were significantly better than after the use of Ixaprep. The same percentage of acrosome-reacted spermatozoa was observed with all three media. Similar results were observed in both normal and subnormal semen samples. CONCLUSION(S):The use of Isolate and Ixaprep resulted in better recovery of motile spermatozoa. Other semen parameters were similar with the use of Isolate and Percoll, whereas the use of Ixaprep was associated with lower sperm velocities and fewer morphologically normal spermatozoa.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Makkar G,Ng HY,Yeung SB,Ho PCdoi
10.1016/s0015-0282(99)00375-1keywords:
subject
Has Abstractpub_date
1999-11-01 00:00:00pages
796-802issue
5eissn
0015-0282issn
1556-5653pii
S0015-0282(99)00375-1journal_volume
72pub_type
杂志文章abstract::In young women receiving chemotherapy for Hodgkin's disease, the combined use of triptorelin and tibolone cotreatment may be a useful tool for preserving ovarian function because all but three (10%) of the women in this treatment group returned to spontaneous ovulation and menses, in contrast to 23% of subjects in the...
journal_title:Fertility and sterility
pub_type: 信件
doi:10.1016/j.fertnstert.2006.10.004
更新日期:2007-03-01 00:00:00
abstract::The histologic appearance of the testes of men exposed to chronic gonadotropin-releasing hormone agonist (GnRH-A) therapy has not been previously documented. Herein, we report on the histologic features of the testes of four patients with disseminated prostatic carcinoma who received at least 1 year of daily treatment...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48205-1
更新日期:1984-11-01 00:00:00
abstract:OBJECTIVE:To compare the laparoscopic excision of primary versus recurrent ovarian endometriomas. DESIGN:Descriptive study. SETTING:Tertiary referral center for the treatment of endometriosis. PATIENT(S):Between 1993 and 2002, 359 consecutive patients: 305 primary surgeries (group A) and 54 reoperations for a recurr...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2005.08.028
更新日期:2006-03-01 00:00:00
abstract:OBJECTIVE:To investigate the incidence of complications in the use of assisted reproductive technology in the management of infertile couples. DESIGN:Retrospective study. SETTING:The Egyptian IVF & ET Center, Maadi, Cairo, Egypt. PATIENT(S):Two thousand nine hundred twenty-four patients underwent IVF-ET or intracyto...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00250-7
更新日期:1998-10-01 00:00:00
abstract::This study describes maternal and birth outcomes of primiparae aged ≥ 45. High rates of pregnancy complications and poor birth outcomes were found, stressing that the personal risks and ramifications to the health system should be taken into account in establishing obstetric health policy regarding primiparity at adva...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2011.02.031
更新日期:2011-06-30 00:00:00
abstract::A study was undertaken comparing the outcomes of 30 women with infertility due to untreated severe (grade IV) pelvic endometriosis with a comparable series of 28 women whose infertility was caused solely by irreversible tubal disease. There were no significant differences in either the follicular phase or luteal phase...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)60078-x
更新日期:1988-08-01 00:00:00
abstract:OBJECTIVE:To demonstrate whether leptin modulates reproduction by a direct effect within the ovary. DESIGN:Animal model. SETTING:National Key Laboratory of Infertility. ANIMAL(S):Adult female db/db mice. INTERVENTION(S):Adult littermate wild-type (WT) and diabetic (db) leptin receptor (LR) mutant female mice were m...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2012.01.126
更新日期:2012-05-01 00:00:00
abstract::Serum anti-Müllerian hormone concentrations vary significantly over time and this should be taken into account when tailoring treatment protocols for patients undergoing controlled ovarian hyperstimulation (COH). Compared with FSH, serum anti-Müllerian hormone may have greater discriminatory power because of its modes...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.10.001
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVE:To determine if increases in core body temperature preceding most hot flashes are caused by increased metabolic rate, peripheral vasoconstriction, or central noradrenergic activation. DESIGN:Laboratory physiological study. SETTING:University medical center. PATIENT(S):Fourteen healthy, postmenopausal women...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/s0015-0282(98)00137-x
更新日期:1998-08-01 00:00:00
abstract:OBJECTIVE:To assess the association between transsexualism and allele and genotype frequencies of the common cytochrome P450 (CYP) 17 -34 T>C single nucleotide polymorphism (SNP). DESIGN:Case-control study. SETTING:Academic research institution. PATIENT(S):102 male-to-female (MtF) and 49 female-to-male (FtM) transse...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.05.056
更新日期:2008-07-01 00:00:00
abstract::Twenty-seven patients underwent ultrasonographic and clinical monitoring of a spontaneous cycle so that human chorionic gonadotropin administration could be timed in an attempt to schedule the screening laparoscopy at the optimal time for oocyte aspiration. Inadequate ovarian access was encountered in only 2 of 26 lap...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)46871-8
更新日期:1983-03-01 00:00:00
abstract::The administration of five subcutaneous 250-microgram doses of lutienizing hormone (LH)-releasing hormone (LHRH) at 4-hour intervals, the first injection being given at 8 A.M. on 1 or 2 consecutive days between days 1 and 9 following the LH surge in normal women, shortened the luteal phase from 1 to 4 days in 16 of 17...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)43755-6
更新日期:1979-01-01 00:00:00
abstract:OBJECTIVE:To characterize and contrast protamine levels, chromatin decondensation, chromosome aneuploidy rates, and functional ability of round-headed sperm from two brothers with round-headed sperm syndrome. DESIGN:Analysis of semen samples from siblings with round-headed sperm syndrome and comparison with semen of f...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(98)00498-1
更新日期:1999-03-01 00:00:00
abstract::Nineteen vasectomized men were followed for 21 to 42 months after surgery, and their sera were tested for the presence of HL-A lymphocytoxic antibodies. In a previous study, the sera of two of these men had shown a definite increase in serum reactivity 6 to 12 months after surgery. Only one of the nineteen tested in t...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)42494-5
更新日期:1977-04-01 00:00:00
abstract:OBJECTIVE:To investigate the localization of tenascin expression in the endometrium of women without endometriosis and in endometriotic implants, and to determine the in vitro regulation of tenascin by E(2) in these tissues. DESIGN:Experimental laboratory study. SETTING:University medical center. PATIENT(S):Reproduc...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.05.028
更新日期:2008-05-01 00:00:00
abstract:OBJECTIVE:To determine the distribution of catalase in eutopic and ectopic endometria in patients with endometriosis or adenomyosis. DESIGN:Retrospective randomized study. SETTING:Department of obstetrics and gynecology in a university hospital. PATIENT(S):Thirty-three patients with endometriosis, 36 with adenomyosi...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(02)03344-7
更新日期:2002-10-01 00:00:00
abstract::Transferrin and somatomedin C receptors (TFr and SMCr) were studied in human ovaries by immunostaining using monoclonal antisera. The oocyte of evoluting follicles was intensely positive for both types of receptors, but this positivity decreases in large follicles. An evident positivity for both TFr and SMCr was shown...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)59533-8
更新日期:1987-11-01 00:00:00
abstract:OBJECTIVE:To enable serodiscordant couples to reproduce while minimizing the risk of infecting the female partner and to demonstrate the feasibility of including these couples in an insemination program. DESIGN:Prospective follow-up study. SETTING:Ambulatory IUIs. PATIENT(S):Fifty-six HIV-1-serodiscordant couples wi...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/j.fertnstert.2004.02.128
更新日期:2004-10-01 00:00:00
abstract:OBJECTIVE:The growth of the follicles induced by gonadotropins during IVF treatment is accompanied by physiologic angiogenesis, which is essential for the maturation of the oocytes. We describe the IVF-induced changes in the vascularization and compare normal with polycystic ovaries (PCOs). DESIGN:Prospective study. ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2004.04.048
更新日期:2004-11-01 00:00:00
abstract:OBJECTIVE:To present the results of assisted reproductive technology (ART) cycles performed in 2004 in Canada. This is the fourth annual report of Canadian ART outcomes. DESIGN:Prospective cohort study. SETTING:Twenty-six of 26 ART centers in Canada. PATIENT(S):Couples undergoing ART treatment in Canada during 2004....
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2007.05.015
更新日期:2008-05-01 00:00:00
abstract:OBJECTIVE:To manipulate the luteal endometrial progression by the use of antiprogestins. DESIGN:Prospective controlled clinical trial. SETTING:The IVF program of the University of Southern California School of Medicine, Los Angeles, California. PATIENT(S):Thirteen oocyte donors and 20 oocyte recipients. INTERVENTIO...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/S0015-0282(97)81918-8
更新日期:1997-02-01 00:00:00
abstract:OBJECTIVE:To investigate whether components of the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway are present and active in human sperm. DESIGN:Comparative study. SETTING:Reproductive biology department. PATIENT(S):Nine sperm donors. INTERVENTION(S):Sperm were exposed to interfer...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(01)01896-9
更新日期:2001-08-01 00:00:00
abstract:OBJECTIVE:To determine the levels of metabolites surrounding the human oocyte and embryo in vivo. DESIGN:Oviduct and uterine fluids were collected throughout the menstrual cycle. Cumulus cells were collected at oocyte retrieval and their production of metabolites was assessed. Samples were analyzed for pyruvate, lacta...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)58097-2
更新日期:1996-02-01 00:00:00
abstract::Embryo transfer is facilitated with ultrasound guidance. The 16 ultrasound-guided transfers were rated easier, and there was less catheter distortion, in comparison with 12 transfers guided by "clinical feel." The benefits we ascribe to ultrasound guidance are as follows: (1) transfers can be done with the patient sup...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)48317-2
更新日期:1985-01-01 00:00:00
abstract::22 physician-providers who serve natural family planning (NFP) programs, mostly in the private sector, gathered to formulate a standard terminology for the field. The Billings and sympto-thermal methods are the 2 methods taught by this group. The sypto-thermal method helps a woman predict ovulation by detecting chan...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)46409-5
更新日期:1982-07-01 00:00:00
abstract::In a prospective randomized fashion, this study evaluated embryo development in vitro after defragmentation versus assisted hatching alone of low grade day 3 embryos. Although a sustained decrease in day 5 fragmentation was observed in the defragmented group versus the assisted hatching only group, no difference in co...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.03.014
更新日期:2010-11-01 00:00:00
abstract:OBJECTIVE:To evaluate the association of an activating single-nucleotide polymorphism (SNP) at position -71 of the promoter of 17beta-hydroxysteroid dehydrogenase type 5 gene (-71A/G HSD17B5 SNP) and polycystic ovary syndrome (PCOS) in a well characterized cohort of caucasian PCOS women with biochemical hyperandrogenem...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2008.06.016
更新日期:2009-08-01 00:00:00
abstract::In ten ovarian endometriomas of diameter exceeding 30 mm, managed by complete vaporization of the inner surface using plasma energy followed by cystectomy, we performed histologic evaluation of the depth of necrosis and the effectiveness of endometrial tissue ablation. We observed that plasma energy allows the ablatio...
journal_title:Fertility and sterility
pub_type: 临床试验,杂志文章
doi:10.1016/j.fertnstert.2010.11.038
更新日期:2011-04-01 00:00:00
abstract::Antiphospholipid syndrome patients with recurrent miscarriage have an impairment in fibrinolysis demonstrated by prolonged clot lysis time (CLT) that cannot be attributed to differences in thrombin activatable fibrinolysis inhibitor (TAFI) antigen levels. Patients with unexplained recurrent miscarriage have an impairm...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/j.fertnstert.2010.02.032
更新日期:2010-11-01 00:00:00
abstract::The purpose of this study was to investigate the prognosis for fertility in children with hydrocele. Our results show that hydrocele seems to be more of a symptom than an actual pathological entity. It occurs both with an open and a closed processus vaginalis. Bilateral hydrocele seems to be a rare occurrence, mostly ...
journal_title:Fertility and sterility
pub_type: 杂志文章
doi:10.1016/s0015-0282(16)53467-0
更新日期:1990-04-01 00:00:00