Abstract:
:The effect of host cell factors on infectivity of human immunodeficiency virus type 1 (HIV-1) was studied by infecting a monoblastoid cell line (U937) or a T-cell line (MOLT-4) with a highly infective single clone of HIV-1 and comparing the infectivity of the produced viruses to different cell lines. Chronically infected U937 cells consistently produced viruses with minimal infectivity. This phenotypic change was host-dependent as the back-passage of the U937-produced low infective viruses into MOLT-4 cells resulted in regaining their original high infectivity. Southern and Northern blot analyses of the HIV-1 grown in U937 cells did not reveal any genomic difference between it and the virus grown it MOLT-4 cells. The radioimmunoprecipitation analysis of viral proteins showed that the HIV-1-infected U937 cells had a different pattern of envelope glycoproteins and core proteins, which well correlated with the low infectivity of the produced viruses. This experimental system using MOLT-4 and U937 cell lines would be useful to further explore host cell factor(s) which play an important role in the regulation of HIV-1 infectivity.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
el-Farrash MA,Masuda T,Kuroda MJ,Harada Sdoi
10.1111/j.1348-0421.1993.tb03221.xsubject
Has Abstractpub_date
1993-01-01 00:00:00pages
349-57issue
5eissn
0385-5600issn
1348-0421journal_volume
37pub_type
杂志文章abstract::Three hundred and twenty-five breast milk samples were examined for the occurrence of human cytomegalovirus (HCMV) by cell culture method. Virus was isolated from the milk in 1 of 177 samples collected within 6 days after delivery, 2 of 115 samples collected during the period of 7 days to 1 month after delivery, 10 of...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1994.tb01862.x
更新日期:1994-01-01 00:00:00
abstract::To clarify the epidemiology of viral acute respiratory infections (ARIs), 305 human parainfluenza virus types 1 (HPIV1), 154 HPIV2 and 574 HPIV3 strains were isolated from 16,962 nasopharyngeal swabs obtained between 2002 and 2011 at pediatric clinics in Yamagata, Japan. The total isolation frequency for HPIV1-3 was 6...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2012.00507.x
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abstract::The spontaneous production of a rat C-type RNA virus (ACV) in a cultured cell line (AC cells) established from a chemically induced rat glioma was studied. The characteristics of ACV were: morphology typical of C-type RNA virus; buoyant density of 1.15 g/ml in a sucrose density gradient; RNA directed DNA polymerase ac...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1979.tb00435.x
更新日期:1979-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2005.tb03649.x
更新日期:2005-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2004.tb03550.x
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1987.tb03155.x
更新日期:1987-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12194
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12357
更新日期:2016-02-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1995.tb02200.x
更新日期:1995-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1988.tb01384.x
更新日期:1988-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1984.tb00722.x
更新日期:1984-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1999.tb02427.x
更新日期:1999-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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journal_title:Microbiology and immunology
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doi:10.1111/j.1348-0421.1996.tb03332.x
更新日期:1996-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12045
更新日期:2013-05-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1983.tb00639.x
更新日期:1983-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1997-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1985.tb02963.x
更新日期:1985-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1996.tb01138.x
更新日期:1996-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1999.tb02375.x
更新日期:1999-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1990.tb01003.x
更新日期:1990-01-01 00:00:00
abstract::A mouse monoclonal antibody, LP4D3, was raised against purified Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) fused to glutathione-S-transferase. The antibody detected endogenous and exogenous EBNA-LP in immunoblotting, immunofluorescence and immunoprecipitation assays, and the epitope of the antibody wa...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2005.tb03743.x
更新日期:2005-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
abstract::Us3 is a serine-threonine protein kinase that is encoded by herpes simplex virus 1 (HSV-1). In experimental animal models of HSV infection, peripheral and intracranial inoculations can be used to study viral pathogenicity in peripheral sites (e.g., eyes and vagina) and central nervous systems (CNSs), respectively. In ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12108
更新日期:2014-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1988.tb01461.x
更新日期:1988-01-01 00:00:00
abstract::Adult T-cell leukemia-associated membrane antigen (ATLMA) expressed on the surface of living ATL virus (ATLV)-carrying cells was investigated by an indirect membrane immunofluorescence method using natural antibodies to ATLV in human sera. All the ATLV-positive cell lines tested that had cytoplasmic ATL-associated ant...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1984.tb00696.x
更新日期:1984-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1987.tb03134.x
更新日期:1987-01-01 00:00:00
abstract::A polymerase chain reaction (PCR) method was performed for rapid and sensitive detection of pathogenic Vibrio trachuri isolated from cultured Japanese horse mackerel. A set of primers was selected from the base sequence of the Pst I fragment of T9210 chromosomal DNA and used for PCR detection of T9210. This PCR specif...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1995.tb03290.x
更新日期:1995-01-01 00:00:00