Physical and functional map of an amikacin-resistance plasmid isolated from a multiresistant strain of Serratia marcescens.

Abstract:

:pTK159, a multiresistance 40-kilobase (kb) plasmid, was isolated from a clinical strain of Serratia marcescens. pTK159 was nonconjugative and carried determinants for resistance to amikacin, streptomycin/spectinomycin, sulfamethoxazole and ampicillin. A physical and functional map of pTK159 was constructed. By cloning various fragments of pTK159 in pACYC184 or pBR322, genes for resistance to amikacin, streptomycin/spectinomycin, and sulfamethoxazole were found to be located on a 2.0-kb BamHI-HindIII fragment, a 1.4-kb HindIII fragment and a 2.1-kb HindIII fragment, respectively. The map of pTK159 was compared with published maps of amikacin-resistance determinants and transposons.

journal_name

Microbiol Immunol

authors

Kanno R

doi

10.1111/j.1348-0421.1988.tb01384.x

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

241-50

issue

3

eissn

0385-5600

issn

1348-0421

journal_volume

32

pub_type

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