Abstract:
:Deep-seated trichosporonosis caused by Trichosporon asahii has a high mortality rate and a very poor prognosis. New species-specific oligonucleotide primers for T. asahii were developed from a sequence analysis of rRNA genes that included the internal transcribed spacer regions. A nested PCR assay with specific primers was used to examine 11 serum samples from 7 patients, who were diagnosed with deep-seated trichosporonosis histologically at autopsy. In addition, Trichosporon cell wall polysaccharide (PS) was detected by a latex agglutination (LA) test. Of 11 samples, seven had a positive LA test, and T. asahii DNA was also detected with the nested PCR assay. Of the four samples in which PS antigen was not detected, the nested PCR of two samples was positive. Our new nested PCR assay may be used as an adjunct to conventional methods for diagnosing T. asahii infection.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Sugita T,Nakajima M,Ikeda R,Niki Y,Matsushima T,Shinoda Tdoi
10.1111/j.1348-0421.2001.tb01282.xkeywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
143-8issue
2eissn
0385-5600issn
1348-0421journal_volume
45pub_type
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