Abstract:
:We recently developed a method for serotyping human rotavirus (HRV) by an enzyme-linked immunosorbent assay with HRV serotype-specific neutralizing monoclonal antibodies (ELISA serotyping). In the present study this method was compared with the fluorescent focus neutralization test with serotype-specific rabbit antisera (NT serotyping) in the sensitivity and specificity of the test. Direct serotyping of HRVs which were contained in stool specimens indicated that while only 37% of the samples were successfully serotyped in NT, 78% of the samples could be serotyped in ELISA. Regarding the samples whose serotype could be determined in the two tests, the assigned serotypes were identical in both tests. The results obtained indicated the utility of ELISA serotyping in clinical and epidemiologic studies of HRV infection.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Urasawa S,Urasawa T,Taniguchi K,Morita Y,Sakurada N,Saeki Y,Morita O,Hasegawa Sdoi
10.1111/j.1348-0421.1988.tb01431.xsubject
Has Abstractpub_date
1988-01-01 00:00:00pages
699-708issue
7eissn
0385-5600issn
1348-0421journal_volume
32pub_type
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