Validity of an enzyme-linked immunosorbent assay with serotype-specific monoclonal antibodies for serotyping human rotavirus in stool specimens.

Abstract:

:We recently developed a method for serotyping human rotavirus (HRV) by an enzyme-linked immunosorbent assay with HRV serotype-specific neutralizing monoclonal antibodies (ELISA serotyping). In the present study this method was compared with the fluorescent focus neutralization test with serotype-specific rabbit antisera (NT serotyping) in the sensitivity and specificity of the test. Direct serotyping of HRVs which were contained in stool specimens indicated that while only 37% of the samples were successfully serotyped in NT, 78% of the samples could be serotyped in ELISA. Regarding the samples whose serotype could be determined in the two tests, the assigned serotypes were identical in both tests. The results obtained indicated the utility of ELISA serotyping in clinical and epidemiologic studies of HRV infection.

journal_name

Microbiol Immunol

authors

Urasawa S,Urasawa T,Taniguchi K,Morita Y,Sakurada N,Saeki Y,Morita O,Hasegawa S

doi

10.1111/j.1348-0421.1988.tb01431.x

subject

Has Abstract

pub_date

1988-01-01 00:00:00

pages

699-708

issue

7

eissn

0385-5600

issn

1348-0421

journal_volume

32

pub_type

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