Prevalence of colonization factor antigens (CFAs) and adherence to HeLa cells in enterotoxigenic Escherichia coli isolated from feces of children in São Paulo.

abstract：:An oral delivery system based on ApxIIA#5-expressed on Saccharomyces cerevisiae was studied for its potential to induce immune responses in mice. Murine bone marrow-derived dendritic cells (DCs) stimulated in vitro with ApxIIA#5-expressed on S. cerevisiae upregulated the expression of maturation and activation markers...

journal_title：Microbiology and immunology

authors： Shin MK,Lee WJ,Jung MH,Cha SB,Shin SW,Yoo A,Kim DH,Yoo HS

更新日期：2013-06-01 00:00:00

abstract：:The effect of dermonecrotic toxin (DNT), fimbrial hemagglutinin (FHA), K-agglutinogen, lipopolysaccharide (LPS), and pertussigen from Bordetella pertussis on the production of IgE and IgG1 antibodies to hen egg albumin (Ea) was investigated in C57BL/6 mice. The IgE antibody contents were determined by passive cutaneou...

journal_title：Microbiology and immunology

authors： Sekiya K

更新日期：1983-01-01 00:00:00

abstract：:A recombinant pertussis DNA vaccine was described here with its immunogenicity and the ability to induce protection against B. pertussis infection in mice. Three immunodominant antigen gene fragments of pertussis, pertussis toxin subunit 1 (pts1), fragments of pertactin (prn) and filamentous hemagglutinin (fha), were ...

journal_title：Microbiology and immunology

authors： Li Q,Zhu Y,Chu J,Wang Y,Xu Y,Hou Q,Zhang S,Guo X

更新日期：2006-01-01 00:00:00

abstract：:The purpose of this study was to sequence the central part of the coding region of the Clostridium tyrobutyricum fiagellin gene to improve the immunoenzymatic counting of cells after milk filtration. The coding region was amplified by PCR, and the amplified products were cloned. A DNA sequence analysis of positive clo...

journal_title：Microbiology and immunology

authors： Arnold F,Bédouet L,Batina P,Robreau G

更新日期：1999-01-01 00:00:00

abstract：:Human immunodeficiency virus type-1 (HIV-1) and human T-cell leukemia virus type-I (HTLV-I) have a similar tropism for target cell types, especially for CD4+ T cells. In this study, we provide evidence that receptors of these two viruses exist independently on the target cell. We established an HTLV-I-producing CD8+ T...

journal_title：Microbiology and immunology

authors： Kannagi M,Kuroda MJ,Maeda Y,Harada S

更新日期：1991-01-01 00:00:00

abstract：:The function of MHC class II HLA-DR molecules expressed on a human B lymphoid progenitor cell line FL8.2.4.4 (abbreviated as FL4.4) was examined. FL4.4 cells expressed HLA-DR molecules and stimulation of the DR molecules by anti-DR mAb or by superantigen TSST-1 induced strong augmentation of homocytic aggregation and ...

journal_title：Microbiology and immunology

authors： Naitoh K,Ichigi Y,Miyake K,Muraguchi A,Kimoto M

更新日期：1994-01-01 00:00:00

abstract：:To clarify the humoral immunity in herpes simplex virus (HSV) infection, HSV-specific IgM, IgA and IgG subclass antibody responses were studied in patients with genital herpes: 17 primary, 13 recurrent and 6 nonprimary first episode. A total of 181 serum samples serially collected from the patients, 5 per patient unti...

journal_title：Microbiology and immunology

authors： Hashido M,Kawana T

更新日期：1997-01-01 00:00:00

abstract：:We performed a cluster analysis of human and animal pathogenic Microsporum species and their teleomorphic states, Arthroderma species, including A. otae-related species (M. canis, M. audouinii, M. distortum, M. equinum, M. langeronii, and M. ferrugineum) and M. gypseum complex (A. fulvum, A. gypseum, and A. incurvatum...

journal_title：Microbiology and immunology

authors： Makimura K,Tamura Y,Murakami A,Kano R,Nakamura Y,Hasegawa A,Uchida K,Yamaguchi H

更新日期：2001-01-01 00:00:00

abstract：:An R-form lipopolysaccharide (LPS) extracted from Klebsiella strain LEN-111 (O3-:K1-) by the phenol-chloroform-petroleum ether method was compared with that extracted by the phenol-water method in the ability to form a hexagonal assembly. The LPS which was extracted by the phenol-water method and dialyzed against tap ...

journal_title：Microbiology and immunology

authors： Kato N,Ohta M,Kido N,Ito H,Naito S,Kuno T

更新日期：1985-01-01 00:00:00

abstract：:CD4+ cytotoxic T lymphocytes (CTL) clones, YT-4 and YT-9, specific for Toxoplasma gondii (T. gondii)-infected melanoma SK-MEL 28 (P36), were generated from the peripheral blood lymphocytes (PBL) of a patient with chronic toxoplasmosis. These CTL clones were shown to secrete significant amounts of interleukin 6 (IL-6) ...

journal_title：Microbiology and immunology

authors： Yang TH,Aosai F,Norose K,Ueda M,Yano A

更新日期：1996-01-01 00:00:00

abstract：:The nucleotide sequence of coxsackievirus B6 (CVB6) has been determined, and the nucleotides encoding the 5' nontranslated region (5' NTR) and virion polypeptides (VP4, 2, 3 and 1) were compared with other serotype CVBs. An Unweighted Pair-Group Method Analysis (UPGMA) of phylogenetic trees indicated that the 5' NTR o...

journal_title：Microbiology and immunology

authors： Kato S,Tsutsumi R,Sato S

更新日期：1999-01-01 00:00:00

abstract：:Although the BCG vaccine can prevent tuberculosis (TB) in infants, its ability to prevent adult pulmonary TB is reportedly limited. Therefore, development of a novel effective vaccine against pulmonary TB has become an international research priority. We have previously reported that intranasal vaccination of mice wit...

journal_title：Microbiology and immunology

authors： Fukui M,Shinjo K,Umemura M,Shigeno S,Harakuni T,Arakawa T,Matsuzaki G

更新日期：2015-12-01 00:00:00

abstract：:Patients with typhoid fever presenting to the Tokyo Metropolitan Komagome Hospital during the period 1975-1998 were retrospectively investigated. All cases were diagnosed by a positive culture for Salmonella typhi in either of their clinical specimens. Of the total number of 130 patients, 57% contracted the disease ab...

journal_title：Microbiology and immunology

authors： Hoshino Y,Masuda G,Negishi M,Ajisawa A,Imamura A,Hachimori K,Takayama N,Yamaguchi T,Kimura M

更新日期：2000-01-01 00:00:00

abstract：:We found a halophilic vibrio in B bile from a 75-year-old female patient with chronic cholecystitis, and examined its biochemical characteristics. The organisms are gram-negative short rods or comma shaped, with some ring forms. They have a single polar flagellum, but not capsule. The strains can grow in peptone water...

journal_title：Microbiology and immunology

authors： Konishi K,Yamagishi T,Sakamoto K

更新日期：1981-01-01 00:00:00

abstract：:There was a significant amount of non-specific, but not of allergen (e.g., papain, mite feces and four kinds of pollen)-specific, IgE antibodies (Abs) in the sera of normal mice. An i.n. injection of each allergen without adjuvant into mice caused an increase in total IgE Ab titers with a similar time course in the se...

journal_title：Microbiology and immunology

authors： Hannya N,Ogita-Nakanishi H,Kato R,Ijiri Y,Hayashi T,Tanaka K,Kawata R,Takenaka H,Kubota T,Yoshida R

更新日期：2018-02-01 00:00:00

abstract：:The polymerase chain reaction (PCR) was applied for the etiological diagnosis of spotted fever group (SFG) rickettsiosis in Japan. Nucleotide primers derived from the 17-kDa antigen gene of Rickettsia rickettsii primed a rickettsia-specific 246-base-pair product for all of the Katayama, Abe, Misaka and Kojima strains,...

journal_title：Microbiology and immunology

authors： Tange Y,Matsumoto M,Okada T,Hasegawa H,Kobayashi Y

更新日期：1994-01-01 00:00:00

abstract：:A serological typing scheme of Bacillus cereus has been developed by immunochemical analyses of flagellar antigen using an agglutination method. Enzyme-linked immunosorbent assay (ELISA) for the classification of flagellar serotype of Bacillus cereus had greater sensitivity. 10-500 times, than that of agglutination me...

journal_title：Microbiology and immunology

authors： Murakami T,Hiraoka K,Mikami T,Matsumoto T,Katagiri S,Suzuki M

更新日期：1991-01-01 00:00:00

abstract：:The purpose of this study was to clarify the taxonomic relationship between all the serovars and species of the genus Erysipelothrix by performing DNA-DNA hybridization experiments, the customary criterion for separation of bacterial genospecies. A total of 93 strains were isolated from a wide variety of sources, incl...

journal_title：Microbiology and immunology

authors： Takahashi T,Fujisawa T,Umeno A,Kozasa T,Yamamoto K,Sawada T

更新日期：2008-10-01 00:00:00

abstract：:A bacterial strain with affinity to Candida albicans was successfully obtained from a natural environment. An uncovered Petri dish containing a suspension of heat-killed C. albicans cells was allowed to stand in a laboratory for several days. Some bacteria which had adhered to the candidal cells were tested for their ...

journal_title：Microbiology and immunology

authors： Seki K,Kobayashi K,Tomiya Y,Sakurada J,Murai M,Usui A,Masuda S

更新日期：1998-01-01 00:00:00

abstract：:Porins were prepared from smooth strain of Salmonella typhi 0-901 and chemotype rough mutant of S. typhimurium Ra-30. Porins could significantly stimulate the immune systems of mice. Immunization of mice with the porins provoked synthesis of anti-porin antibodies. Macrophages from the immunized mice showed increased c...

journal_title：Microbiology and immunology

authors： Tabaraie B,Sharma BK,Sharma PR,Sehgal R,Ganguly NK

更新日期：1994-01-01 00:00:00

abstract：:A mass outbreak of Escherichia coli O157:H45 was first reported in Japan in 1998. This pathogen was classified as an enteropathogenic E. coli (EPEC) O157 because it was characterized by the Shiga toxin gene (stx)-negative and bundle-forming pilus (bfp) gene-positive genotypes. In this study, we investigated the type I...

journal_title：Microbiology and immunology

authors： Abe A,Nagano H

更新日期：2000-01-01 00:00:00

abstract：:B lymphocytes express multiple TLRs that regulate their cytokine production.We investigated the effect of TLR4 and TLR9 activation on receptor activator of NF‐kB ligand (RANKL) expression by rat spleen B cells. Splenocytes or purified spleen B cells from Rowett rats were cultured with TLR4 ligand Escherichia coli LPS ...

journal_title：Microbiology and immunology

authors： Yu X,Lin J,Yu Q,Kawai T,Taubman MA,Han X

更新日期：2014-01-01 00:00:00

abstract：:Actin is a major antigen involved in the reaction of smooth muscle antibody positive sera from patients with chronic active hepatitis. In the present study, actin extracted from rabbit skeletal muscle was denatured by sodium dodecyl sulfate and was immunized into the rabbit, a homologous animal for actin. The rabbits,...

journal_title：Microbiology and immunology

authors： Nishioka M,Aibiki T,Shirai M,Terada S,Kagawa H,Watanabe S

更新日期：1986-01-01 00:00:00

abstract：:Biofilm formation can cause refractory urinary tract infections (UTIs) in dogs; however, minimum biofilm eradication concentrations (MBECs) of veterinary drugs against canine uropathogens remain to be investigated. In this study, the MBECs of orbifloxacin (OBFX), trimethoprim-sulfamethoxazole (TMS) and amoxicillin/cla...

journal_title：Microbiology and immunology

authors： Shimizu T,Harada K

更新日期：2017-01-01 00:00:00

abstract：:The coat protein of cardamom mosaic virus (CdMV), a member of the genus Macluravirus, assembles into virus-like particles when expressed in an Escherichia coli expression system. The N and C-termini of the coat protein were engineered with the Kennedy peptide and the 2F5 and 4E10 epitopes of gp41 of HIV. The chimeric ...

journal_title：Microbiology and immunology

authors： Damodharan S,Gujar R,Pattabiraman S,Nesakumar M,Hanna LE,Vadakkuppattu RD,Usha R

更新日期：2013-05-01 00:00:00

abstract：:Common EDTA-soluble antigens of Clostridium chauvoei and C. septicum were examined by indirect-immunofluorescence (IFA) and immunoblot analysis. The monoclonal antibodies (mAbs) specific for the 35 kDa antigen of C. chauvoei strain ATCC 10092 were used. These mAbs reacted with all 11 strains, 6 of C. chauvoei and 5 of...

journal_title：Microbiology and immunology

authors： Hamaoka T,Terakado N,Nakamura S

更新日期：1994-01-01 00:00:00

abstract：:A mouse monoclonal antibody, LP4D3, was raised against purified Epstein-Barr virus nuclear antigen leader protein (EBNA-LP) fused to glutathione-S-transferase. The antibody detected endogenous and exogenous EBNA-LP in immunoblotting, immunofluorescence and immunoprecipitation assays, and the epitope of the antibody wa...

journal_title：Microbiology and immunology

authors： Shaku F,Matsuda G,Furuya R,Kamagata C,Igarashi M,Tanaka M,Kanamori M,Nishiyama Y,Yamamoto N,Kawaguchi Y

更新日期：2005-01-01 00:00:00

abstract：:Staphylococcus aureus Cowan I was incubated with monolayers of cells derived from several portions of mouse kidney, and found to be ingested by all types of the renal cells. Intracellular localization of S. aureus was determined by resistance of intracellular cocci against lysostaphin digestion and confirmed by electr...

journal_title：Microbiology and immunology

authors： Murai M,Usui A,Seki K,Sakurada J,Masuda S

更新日期：1992-01-01 00:00:00

abstract：:The mechanisms of B cell tolerance were studied in an attempt to learn whether B cells rendered tolerant are present in the immune system in a potentially responsive form. The author tested the in vitro anti-trinitrophenyl (TNP) antibody-forming cell (anti-TNP AFC) response to TNP-immunogens and polyclonal B cell acti...

journal_title：Microbiology and immunology

authors： Uchiyama T

更新日期：1980-01-01 00:00:00

abstract：:Salmonella typhimurium strain 9 produces mini-cells during cell proliferation. Mini-cells are viable but cannot proliferate since they do not contain chromosomal DNA. Effects of whole cells and mini-cells of S. typhimurium on the immune responses were investigated, with the following results. Phagocytosis of peritonea...

journal_title：Microbiology and immunology

authors： Kurashige S,Yoshida T,Kodama K,Mitsuhashi S

更新日期：1981-01-01 00:00:00