Immunopotentiating activity of whole cells and mini-cells of Salmonella typhimurium.

abstract：:We established an assay system for detecting T cell-replacing factor (TRF) activity of translated materials in Xenopus oocytes of poly (A)-positive mRNA extracted from a T cell hybrid cell line, B151K12 (B151) which constitutively produces TRF. Since it was difficult to detect TRF activity of the translated products o...

journal_title：Microbiology and immunology

authors： Tominaga A,Matsumoto M,Takahashi T,Harada N,Takatsu K

更新日期：1986-01-01 00:00:00

abstract：:We constructed a plasmid containing bovine leukemia virus (BLV)-tax gene driven by SR alpha promoter, designated as pME-BLVtax, to activate the promoter of the long terminal repeat (LTR) of BLV in various tumor cells. Activation of the promoter of BLV-LTR by pME-BLVtax was confirmed by luciferase assay. When the cells...

journal_title：Microbiology and immunology

authors： Tana,Watarai S,Aida Y,Tajima S,Kakidani H,Onuma M,Kodama H

更新日期：2001-01-01 00:00:00

abstract：:Alkaline phosphatase-conjugated oligonucleotide probes were developed to detect the gene coding for Vero toxin 1 (VT1) and Vero toxin 2 (VT2). Using these probes, 3 hr was enough to detect VT genes when suspicious colonies of enterohaemorrhagic Escherichia coli (EHEC) were obtained on an agar plate. The results of a h...

journal_title：Microbiology and immunology

authors： Yoh M,Takagi K,Eda J,Ohtomo M,Takarada Y,Shibata S,Honda T

更新日期：1997-01-01 00:00:00

abstract：:Blastomycosis, caused by the thermally dimorphic fungus Blastomyces dermatitides, which is endemic to eastern regions of the USA, is commonly misdiagnosed as a viral or bacterial infection and therefore treated improperly. Over the years, many immunodiagnostic assays to aid in the diagnosis of blastomycosis have been ...

journal_title：Microbiology and immunology

authors： Hatch WO,Scalarone GM

更新日期：2013-11-01 00:00:00

abstract：:Vpr, one of the accessory molecules of HIV-1, has been demonstrated to arrest the cell cycle at the G2 phase. This Vpr-mediated cell cycle arrest is implicated to have an important role in the viral life cycle. In the present study, we quantitate the extent of Vpr-mediated cell cycle arrest with the use of a bicistron...

journal_title：Microbiology and immunology

authors： Yamaguchi T,Watanabe N,Nakauchi H,Koito A

更新日期：1999-01-01 00:00:00

abstract：:The amino acid sequence of the principal neutralizing determinant (PND) of 224 cases of human immunodeficiency virus type 1 (HIV-1) was determined and the most frequently occurring sequence was used as a peptide antigen for studying virus-specific antibody responses. In our present study, a linear peptide of the most ...

journal_title：Microbiology and immunology

authors： Bukawa H,Fukushima J,Hamajima K,Kimura M,Tsuji T,Xin KQ,Okuda K

更新日期：1995-01-01 00:00:00

abstract：:Fifty-eight enterotoxigenic Escherichia coli (ETEC) strains, isolated from children with and without diarrhea in São Paulo, were examined for the presence of colonization factor antigens (CFAs) and their ability to adhere to HeLa cells. Antisera to CFA/I, the coli surface (CS) antigens CS1CS3, CS2CS3, and CS2 of CFA/I...

journal_title：Microbiology and immunology

authors： Guth BE,Aguiar EG,Griffin PM,Ramos SR,Gomes TA

更新日期：1994-01-01 00:00:00

abstract：:RobA is a member of the XylS/AraC subfamily of DNA binding proteins, and when overexpressed, it induces multiple antibiotic resistance in Escherichia coli. In this study, we introduced a multicopy robA plasmid (pMEP1) and its derivative into OmpF mutants and an AcrAB-deficient mutant. We found that a decrease in susce...

journal_title：Microbiology and immunology

authors： Tanaka T,Horii T,Shibayama K,Sato K,Ohsuka S,Arakawa Y,Yamaki K,Takagi K,Ohta M

更新日期：1997-01-01 00:00:00

abstract：:Hemadsorption (HAD) induced in HEp-2 cells infected with vaccinia virus was observed. In ultrathin sections, binding of 36 red blood cells (RBCs) was examined in detail and 3 types of HAD were observed: (1) direct and close binding of RBCs to infected HEp-2 cells (cyto-HAD) was observed in cross sections of 27 RBCs (2...

journal_title：Microbiology and immunology

authors： Saburi Y,Okuda K,Takahashi T,Tadokoro I

更新日期：1977-10-20 00:00:00

abstract：:Temperature dependence of transfer was examined with ten R plasmids originating from clinical isolates of Salmonella. Six of the plasmids were thermosensitive upon transfer, five of which were originally harbored in S. typhimurium and the remaining one in S. derby. One of these plasmids, pNR502, which conferred resist...

journal_title：Microbiology and immunology

authors： Yoshida Y,Terawaki Y,Nakaya R

更新日期：1978-01-01 00:00:00

abstract：:Porphyromonas gingivalis autolyzes in the culture media. To examine in more detail the molecular components of the autolysate, two-dimensional gel electrophoresis was performed. Many protein spots varied both in number and volume. One of these spots included Arg-gingipain (Rgp) as determined by N-terminal amino acid s...

journal_title：Microbiology and immunology

authors： Kamaguchi A,Nakano M,Shoji M,Nakamura R,Sagane Y,Okamoto M,Watanabe T,Ohyama T,Ohta M,Nakayama K

更新日期：2004-01-01 00:00:00

abstract：:Accurate and rapid detection of carbapenemases and identification of their types in Enterobacteriaceae are both still major challenges for clinical laboratories in attempting to prevent the intrusion and transmission of carbapenemase-producing Enterobacteriaceae. This study aimed to evaluate the performance of the MAS...

journal_title：Microbiology and immunology

authors： Ohsaki Y,Kubo R,Hobson J,Davies M,Osaka S,Hayashi W,Taniguchi Y,Koide S,Nagano Y,Nagano N

更新日期：2018-01-01 00:00:00

abstract：:The lack of a suitable animal model is a major obstacle to developing anti-HIV-1 vaccines. We successfully generated an SIVmac/HIV-1 chimeric virus (SHIV) (designated as NM-3rN) that contains the HIV-1 env gene and is infectious to macaque monkeys. Challenging the vaccinated macaque monkeys with NM-3rN, we developed a...

journal_title：Microbiology and immunology

authors： Haga T,Kuwata T,Ui M,Igarashi T,Miyazaki Y,Hayami M

更新日期：1998-01-01 00:00:00

abstract：:Autoantibodies to IgE and beta2-adrenergic receptor have been reported in patients with allergic rhinitis. To investigate whether autoimmunity in allergic rhinitis is directed to such limited molecules or directed to a wide range of self proteins, we here attempted to survey autoantigens/autoantibodies comprehensively...

journal_title：Microbiology and immunology

authors： Nakamura M,Tsutsumi K,Ooka S,Sekine T,Koizuka I,Nishioka K,Kato T

更新日期：2004-01-01 00:00:00

abstract：:Human immunodeficiency virus type-1 (HIV-1) and human T-cell leukemia virus type-I (HTLV-I) have a similar tropism for target cell types, especially for CD4+ T cells. In this study, we provide evidence that receptors of these two viruses exist independently on the target cell. We established an HTLV-I-producing CD8+ T...

journal_title：Microbiology and immunology

authors： Kannagi M,Kuroda MJ,Maeda Y,Harada S

更新日期：1991-01-01 00:00:00

abstract：:B. pertussis is a causative agent of whooping cough (pertussis) in humans. Despite wide-scale vaccination in many countries, there is serious concern about pertussis as a re-emerging disease. Re-emergence of pertussis may be explained by several factors: the short duration of protection by the currently available acel...

journal_title：Microbiology and immunology

authors： Abe A,Nagamatsu K,Watanabe M

更新日期：2008-02-01 00:00:00

abstract：:This study revealed the presence of various rickettsial agents in mites from wild rodents collected in Southern Jeolla Province, Korea, by nested polymerase chain reaction (PCR) and sequence analysis of a partial citrate synthase and rickettsia outer membrane protein B genes. Rickettsial agents closely related to the ...

journal_title：Microbiology and immunology

authors： Choi YJ,Lee EM,Park JM,Lee KM,Han SH,Kim JK,Lee SH,Song HJ,Choi MS,Kim IS,Park KH,Jang WJ

更新日期：2007-01-01 00:00:00

abstract：:The attachment of Mycoplasma pulmonis m53 organisms to mouse and rat synovial cells was examined by using the organisms and the synovial cells treated in various ways. M. pulmonis treated with trypsin attached to the synovial cells, but the organisms treated with pronase, formaldehyde, glutaraldehyde, or heat did not....

journal_title：Microbiology and immunology

authors： Araake M,Yayoshi M,Yoshioka M

更新日期：1985-01-01 00:00:00

abstract：:A mass outbreak of Escherichia coli O157:H45 was first reported in Japan in 1998. This pathogen was classified as an enteropathogenic E. coli (EPEC) O157 because it was characterized by the Shiga toxin gene (stx)-negative and bundle-forming pilus (bfp) gene-positive genotypes. In this study, we investigated the type I...

journal_title：Microbiology and immunology

authors： Abe A,Nagano H

更新日期：2000-01-01 00:00:00

abstract：:The nucleotide sequence of coxsackievirus B6 (CVB6) has been determined, and the nucleotides encoding the 5' nontranslated region (5' NTR) and virion polypeptides (VP4, 2, 3 and 1) were compared with other serotype CVBs. An Unweighted Pair-Group Method Analysis (UPGMA) of phylogenetic trees indicated that the 5' NTR o...

journal_title：Microbiology and immunology

authors： Kato S,Tsutsumi R,Sato S

更新日期：1999-01-01 00:00:00

abstract：:The production from murine resident peritoneal macrophages (M phi) of a soluble factor, which was capable of enhancing the antigen-presenting (AP) function of dendritic cells (DC), was examined. The supernatants of peritoneal M phi (M phi sup) were prepared by culturing peritoneal M phi with particles, i.e., zymosan A...

journal_title：Microbiology and immunology

authors： Miyazaki H,Ito A,Osawa T

更新日期：1988-01-01 00:00:00

abstract：:This study aimed to investigate the antifungal activity of Rubus chingii extract in combination with fluconazole (FLC) against FLC-resistant Candida albicans 100 in vitro. A R. chingii extract and FLC-resistant C. albicans fungus suspension were prepared. The minimum inhibitory concentration and fractional inhibitory ...

journal_title：Microbiology and immunology

authors： Han B,Chen J,Yu YQ,Cao YB,Jiang YY

更新日期：2016-02-01 00:00:00

abstract：:Mice primed with 1 microgram of reduced and alkylated ovalbumin (RA-OA) developed not only long-lived memory cells for delayed-type hypersensitivity (DTH), capable of differentiating into DTH-effector T cells (DTH-Te) against ovalbumin (OA) when restimulated in vitro with OA, but also spleen cells capable of augmentin...

journal_title：Microbiology and immunology

authors： Kojima A,Tamura SI,Egashira Y

更新日期：1982-01-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) was reactivated more rapidly in cells of latently infected mouse trigeminal ganglia which were cultured in serum-free medium (after 3.7 days of cultivation) than in those cultured in serum-containing Dulbecco's modified Eagle's medium (after 8.5 days of cultivation). The concentrati...

journal_title：Microbiology and immunology

authors： Kimura S

更新日期：1996-01-01 00:00:00

abstract：:Dengue virus multiplication in cultures of a murine myelomonocytic cell line (WEHI-3) as well as mouse peritoneal macrophages was enhanced by treatment of the cells with lipophilic derivatives of muramyl peptides for 2 or 3 days before virus inoculation, but not for 2 hr before virus inoculation or during the adsorpti...

journal_title：Microbiology and immunology

authors： Hotta H,Sanchez LF,Takada H,Homma M,Kotani S

更新日期：1985-01-01 00:00:00

abstract：:Triosephosphate isomerase (TPI; EC 5. 3. 1. 1) displayed on the cell surface of Staphylococcus aureus acts as an adhesion molecule that binds to the capsule of Cryptococcus neoformans, a fungal pathogen. This study investigated the function of TPI on the cell surface of S. aureus and its interactions with biological s...

journal_title：Microbiology and immunology

authors： Furuya H,Ikeda R

更新日期：2011-12-01 00:00:00

abstract：:Staphylococcus aureus Cowan I was incubated with monolayers of cells derived from several portions of mouse kidney, and found to be ingested by all types of the renal cells. Intracellular localization of S. aureus was determined by resistance of intracellular cocci against lysostaphin digestion and confirmed by electr...

journal_title：Microbiology and immunology

authors： Murai M,Usui A,Seki K,Sakurada J,Masuda S

更新日期：1992-01-01 00:00:00

abstract：:Using anti-human B cell monoclonal antibodies prepared against B1 (CD20), B2 (CD21), B4 (CD19), and BB-1 (B lymphoblast antigen-1), we compared the expression of B cell differentiation antigens on a Jijoye-P3HR-1 cell line family of Burkitt's lymphomas. The expression of BB-1 and B2 antigens was faint on P3HR-1 K cell...

journal_title：Microbiology and immunology

authors： Yokochi T,Inoue Y,Iwata H,Miyadai T,Kimura Y

更新日期：1988-01-01 00:00:00

abstract：:Fibronectin is known to be an important factor in colonization by Staphylococcus aureus of host tissues as well as other extracellular matrix proteins such as collagen and laminin. We investigated the effect of fibronectin on the phagocytosis of the S. aureus Cowan I strain by macrophages and of coagulase-negative sta...

journal_title：Microbiology and immunology

authors： Shinji H,Sakurada J,Seki K,Murai M,Masuda S

更新日期：1998-01-01 00:00:00

abstract：:Macrophages (Mphis), but not T cells, infiltrating into the rejection site of either i.p. allografted Meth A (H-2d) fibrosarcoma cells in C57BL/6 (B6) (H-2b) mice or BALB/c (H-2d) skin onto B6 mice are cytotoxic against allografts with H-2d specificity. To determine the mechanisms of specific killing of allografts by ...

journal_title：Microbiology and immunology

authors： Yoshida R,Oku T,Takikawa O,Einaga-Naito K,Yoneda Y,Hirota R,Kubota T

更新日期：2000-01-01 00:00:00