Herpes simplex virus-specific IgM, IgA and IgG subclass antibody responses in primary and nonprimary genital herpes patients.

abstract：:The effect of host cell factors on infectivity of human immunodeficiency virus type 1 (HIV-1) was studied by infecting a monoblastoid cell line (U937) or a T-cell line (MOLT-4) with a highly infective single clone of HIV-1 and comparing the infectivity of the produced viruses to different cell lines. Chronically infec...

journal_title：Microbiology and immunology

authors： el-Farrash MA,Masuda T,Kuroda MJ,Harada S

更新日期：1993-01-01 00:00:00

abstract：:The Fr/V and Oc/V sibling viruses of the Osaka-1 strain of the subacute sclerosing panencephalitis (SSPE) virus were defective in cell-free virus production. By radioimmunoprecipitation assay, the matrix (M) protein was not detected in cells persistently infected with the Osaka-1 strain. This undetectable expression w...

journal_title：Microbiology and immunology

authors： Ayata M,Hayashi K,Seto T,Murata R,Ogura H

更新日期：1998-01-01 00:00:00

abstract：:Natural killer cells, a critical component of the innate immune system, eradicate both virus-infected cells and tumor cells through cytotoxicity and secretion of cytokines. Human NK cell research has largely been based on in vitro studies because of the lack of appropriate animal models. In this study, a selective pro...

journal_title：Microbiology and immunology

authors： Hattori S,Matsuda K,Kariya R,Harada H,Okada S

更新日期：2016-02-01 00:00:00

abstract：:β-1,3-glucan is a major component of fungal cell walls with various biological activities, including effects on the production of inflammatory mediators in vivo and in vitro. However, few reports have examined its influence on the fungal cell itself. In this study, the influences of β-1,3-glucan on the growth and cell...

journal_title：Microbiology and immunology

authors： Ishibashi K,Kurone Y,Motoi M,Miura NN,Adachi Y,Shirasu Y,Ohno N

更新日期：2010-11-01 00:00:00

abstract：:We previously reported the establishment of an HEp2 cell line which expresses the US3 protein kinase (PK) of herpes simplex virus type 2 (HSV-2) upon induction with IPTG. Here we report that expression, phosphorylation and ubiquitination of cytokeratin 17 (CK17) are enhanced in US3-expressing HEp2 cells. In vitro kina...

journal_title：Microbiology and immunology

authors： Murata T,Goshima F,Nishizawa Y,Daikoku T,Takakuwa H,Ohtsuka K,Yoshikawa T,Nishiyama Y

更新日期：2002-01-01 00:00:00

abstract：:Cholesterol is necessary for the conversion of Vibrio cholerae hemolysin (VCH) monomers into oligomers in liposome membranes. Using different sterols, we determined the stereochemical structures of the VCH-binding active groups present in cholesterol. The VCH monomers are bound to cholesterol, diosgenin, campesterol, ...

journal_title：Microbiology and immunology

authors： Ikigai H,Otsuru H,Yamamoto K,Shimamura T

更新日期：2006-01-01 00:00:00

abstract：:A high molecular weight proteinaceous factor in the cell extract of sarcoma 180 (S-180) was found to inhibit phorbol myristate acetate (PMA)-triggering of macrophage H2O2 release. This factor (S-180 factor) was stable at 56 C for 1 hr and resistant to ultraviolet-irradiation. The S-180 factor inhibited the specific bi...

journal_title：Microbiology and immunology

authors： Tomioka H,Saito H

更新日期：1985-01-01 00:00:00

abstract：:proteins which were able to bind noncovalently with mouse factor B were found in cells that are nonsecretors of factor B such as mouse-established monocytic cells and L cells but not in peritoneal resident macrophages. These proteins were isolated from lysates of L cells and separated into four distinct proteins by pr...

journal_title：Microbiology and immunology

authors： Moriyama T,Kawamoto Y,Ichikawa H,Okamoto K,Inoue T,Kobayashi T,Miyama A

更新日期：1983-01-01 00:00:00

abstract：:One strain each of granular and downy varieties of Trichophyton mentagrophytes and two strains each of granular and floccose colonial types Trichophyton rubrum were used for preparation of antigens. The antigens were analyzed by double diffusion against homologous and heterologous antisera raised in rabbits. A total o...

journal_title：Microbiology and immunology

authors： Shukla NP,Agarwal GP

更新日期：1983-01-01 00:00:00

abstract：:In order to assess the role of the route of immunization on the immunogenicity of killed Salmonella vaccine, mice were immunized with killed S. enteritidis by intraperitoneal (i.p.) and intradermal (i.d.) routes. Whereas the former was non-immunogenic, the i.d. immunization generated an excellent delayed-type hypersen...

journal_title：Microbiology and immunology

authors： George A,Shroff KE,Rath S,Ghosh SN,Sengupta SR,Kamat RS

更新日期：1989-01-01 00:00:00

abstract：:Common EDTA-soluble antigens of Clostridium chauvoei and C. septicum were examined by indirect-immunofluorescence (IFA) and immunoblot analysis. The monoclonal antibodies (mAbs) specific for the 35 kDa antigen of C. chauvoei strain ATCC 10092 were used. These mAbs reacted with all 11 strains, 6 of C. chauvoei and 5 of...

journal_title：Microbiology and immunology

authors： Hamaoka T,Terakado N,Nakamura S

更新日期：1994-01-01 00:00:00

abstract：:CD4+ cytotoxic T lymphocytes (CTL) clones, YT-4 and YT-9, specific for Toxoplasma gondii (T. gondii)-infected melanoma SK-MEL 28 (P36), were generated from the peripheral blood lymphocytes (PBL) of a patient with chronic toxoplasmosis. These CTL clones were shown to secrete significant amounts of interleukin 6 (IL-6) ...

journal_title：Microbiology and immunology

authors： Yang TH,Aosai F,Norose K,Ueda M,Yano A

更新日期：1996-01-01 00:00:00

abstract：:To determine the infection source of a sporadic Legionella pneumonia case associated with a hot spring bath, we used five molecular methods, including repetitive element polymerase chain reaction (rep-PCR), arbitrarily primed PCR (AP-PCR), ribotyping, restriction endonuclease analysis (REA), and macrorestriction endon...

journal_title：Microbiology and immunology

authors： Miyamoto H,Jitsurong S,Shiota R,Maruta K,Yoshida S,Yabuuchi E

更新日期：1997-01-01 00:00:00

abstract：:Bacteria of Stenotrophomonas maltophilia have been isolated with increasing frequency from the airways of cystic fibrosis (CF) patients, usually following P. aeruginosa infections, but their adherence to human epithelial respiratory cells has never been investigated. In this study, various S. maltophilia strains were ...

journal_title：Microbiology and immunology

authors： De Vidipó LA,De Marques EA,Puchelle E,Plotkowski MC

更新日期：2001-01-01 00:00:00

abstract：:Streptococcus suis infection induces formation of neutrophil extracellular traps (NETs) in vitro; however, the contribution of NETs-mediated killing to the pathogenesis of S. suis in vivo is yet to be elicited. The findings of the present study indicated that extracellular DNA fiber can be induced in a murine model in...

journal_title：Microbiology and immunology

authors： Zhao J,Lin L,Fu L,Han L,Zhang A

更新日期：2016-04-01 00:00:00

abstract：:Absorption of a pooled human gamma globulin preparation with acetone-treated measles virus-infected cells removed all antibodies to measles virus antigens except a portion of the antibody to the fusion (F) protein. The residual anti-F antibody had hemolysis-inhibiting and virus-neutralizing activities, inhibited sprea...

journal_title：Microbiology and immunology

authors： Sato TA,Kohama T,Sugiura A

更新日期：1989-01-01 00:00:00

abstract：:We developed the antigen capture enzyme-linked immunosorbent assay (ELISA) systems for quantification of Borna disease virus (BDV) major antigens, p40 and p24. Using these ELISAs, we quantified the two proteins in various BDV-infected materials, including the cell lysates and culture supernatants as well as the homoge...

journal_title：Microbiology and immunology

authors： Watanabe M,Zhong Q,Kobayashi T,Kamitani W,Tomonaga K,Ikuta K

更新日期：2000-01-01 00:00:00

abstract：:Salmonella typhimurium strain 9 produces mini-cells during cell proliferation. Mini-cells are viable but cannot proliferate since they do not contain chromosomal DNA. Effects of whole cells and mini-cells of S. typhimurium on the immune responses were investigated, with the following results. Phagocytosis of peritonea...

journal_title：Microbiology and immunology

authors： Kurashige S,Yoshida T,Kodama K,Mitsuhashi S

更新日期：1981-01-01 00:00:00

abstract：:When the R-form lipopolysaccharide (LPS) from Klebsiella pneumoniae strain LEN-111 (O3-:K1-), from which cationic material had been removed by electrodialysis, was suspended in 50 mM Tris buffer at pH 8.5 containing 0.1 mM or higher concentrations of MgCl2, it formed an ordered two-dimensional hexagonal lattice struct...

journal_title：Microbiology and immunology

authors： Kato N,Ohta M,Kido N,Arakawa Y,Ito H,Naito S

更新日期：1990-01-01 00:00:00

abstract：:The major structural proteins of Newcastle disease virus and Sendai virus were localized in infected BHK-21 and MDBK cells by ultrastructural immunoperoxidase cytochemistry using antibodies against the individual viral protein antigens. The intracellular glycoproteins were strictly membrane bound, being localized in t...

journal_title：Microbiology and immunology

authors： Nagai Y,Yoshida T,Hamaguchi M,Nagura H,Hasegawa H,Yoshimura S,Watanabe K

更新日期：1983-01-01 00:00:00

abstract：:Alpha-fetoprotein (AFP) expression is observed in embryonic tissues and, the expression of this protein is absent in normal adult tissues. The re-elevation of serum AFP strongly suggests generation of a malignant tumor in an adult. We demonstrated here that AFP-producing gastric cancer (AFP-gastric cancer) could be tr...

journal_title：Microbiology and immunology

authors： Miura Y,Kataoka H,Joh T,Tada T,Asai K,Nakanishi M,Okada N,Okada H

更新日期：2004-01-01 00:00:00

abstract：:Amino acid requirements for the growth of Aedes albopictus, clone C6/36, cells and for the production of dengue (DEN) and Chikungunya (CHIK) viruses were examined by growing the cells or the viruses in media which were deprived of one of the 20 amino acids. Cell growth was markedly inhibited when cystine was omitted f...

journal_title：Microbiology and immunology

authors： Sasao F,Igarashi A,Fukai K

更新日期：1980-01-01 00:00:00

abstract：:Cross-linking of surface receptors in hematopoietic cells results in the enrichment of these receptors in the rafts along with other downstream signaling molecules. A possible explanation how signal is transduced through the plasma membrane has arisen from the concept of raft. From the study of cellular responses in t...

journal_title：Microbiology and immunology

authors： Katagiri YU,Kiyokawa N,Fujimoto J

更新日期：2001-01-01 00:00:00

abstract：:The use of continuous free-flow electrophoresis for the purification of extracted lipopolysaccharides ( LPSs ) was investigated. Commercial (nucleic acid contaminated) LPS preparations, isolated by the hot phenol-water method of Westphal from Salmonella typhimurium and Escherichia coli 0111: B4, were analyzed. Continu...

journal_title：Microbiology and immunology

authors： Kuwae T,Kurata M

更新日期：1984-01-01 00:00:00

abstract：:We developed a rapid and simple method for the screening of antiviral agents against herpes simplex virus (HSV) in a model of gastrointestinal herpetic infection in vitro. This method was based on inhibition of HSV-induced cytopathogenicity in gastric adenocarcinoma MKN-28 cells, as monitored by an MTT colorimetric as...

journal_title：Microbiology and immunology

authors： Kodama E,Igarashi A,Mori S,Hashimoto K,Suzuki T,De Clercq E,Shigeta S

更新日期：1996-01-01 00:00:00

abstract：:The protective role of neutrophils on intranasal infection of influenza virus was investigated in 3 strains of tumor-bearing mice with neutrophilic leukocytosis. In vitro multiplication of influenza virus was inhibited by neutrophils from both normal and tumor-bearing mice, and the inhibitory effect of neutrophils was...

journal_title：Microbiology and immunology

authors： Fujisawa H

更新日期：2001-01-01 00:00:00

abstract：:Direct stimulations of murine B lymphocytes with synthetic lipid A analogs and synthetic muramyl dipeptide (MDP) derivatives were studied using a limiting dilution assay system. Synthetic lipid A analogs, GLA-27 and GLA-40, when conjugated with bovine serum albumin (BSA) had the ability to induce B cell clonal expansi...

journal_title：Microbiology and immunology

authors： Saito-Taki T,Nakano M,Kiso M,Hasegawa A

更新日期：1985-01-01 00:00:00

abstract：:We investigated sex- and age-specific prevalences of hepatitis B e antigen (HBeAg) and antibody to HBeAg (anti-HBe) in 1,163 carriers of hepatitis B surface antigen (HBsAg) in Okinawa, Japan, and followed them up for longer than one year in correlation with liver function abnormalities. Elevated serum transaminase lev...

journal_title：Microbiology and immunology

authors： Hayashi J,Kashiwagi S,Ikematsu H,Nomura H,Kajiyama W,Noguchi A,Ikeda K

更新日期：1988-01-01 00:00:00

abstract：:Biofilm formation can cause refractory urinary tract infections (UTIs) in dogs; however, minimum biofilm eradication concentrations (MBECs) of veterinary drugs against canine uropathogens remain to be investigated. In this study, the MBECs of orbifloxacin (OBFX), trimethoprim-sulfamethoxazole (TMS) and amoxicillin/cla...

journal_title：Microbiology and immunology

authors： Shimizu T,Harada K

更新日期：2017-01-01 00:00:00

abstract：:Bacterial images can be obtained rather easily with an atomic-force microscope (AFM) in the magnification range of 5,000 to 30,000 times without any pretreatment of the specimens for such observations as chemical fixation, dehydration or staining. The bacterial shapes or the presence of flagella can be clearly recogni...

journal_title：Microbiology and immunology

authors： Umeda A,Saito M,Amako K

更新日期：1998-01-01 00:00:00