Rapid purification of extracted bacterial lipopolysaccharides by continuous free-flow electrophoresis.

abstract：:Our objective was to study the alterations of CD4(+) CD25(+) Foxp3(+) T(regs) in HIV-infected SPs and to examine the role of T(regs) in the disease progression of HIV. The proportion of CD4(+) CD25(+) Foxp3(+) T(regs) in peripheral blood of 24 SPs, 30 asymptomatic HIV-infected patients, 20 AIDS patients, and 16 non-in...

journal_title：Microbiology and immunology

authors： Zhang Z,Jiang Y,Zhang M,Liu J,Sun G,Shi W,Wang Y,Shang H

更新日期：2010-10-01 00:00:00

abstract：:No systematic study on serotyping of Enterococcus faecalis has been reported since 1964 when M.E. Sharpe conducted serotyping of group D streptococcus in U.K. So, we attempted to re-evaluate serotyping of E. faecalis. For this purpose, we received 42 Sharpe's strains and first examined for their biochemical characteri...

journal_title：Microbiology and immunology

authors： Maekawa S,Yoshioka M,Kumamoto Y

更新日期：1992-01-01 00:00:00

abstract：:We developed an effective system to eliminate poliovirus from modified tap water using a positively-charged carbon felt electrode. The zeta potential of polioviruses was measured using laser microscopic electrophoresis. Poliovirus adsorption to the electrode was examined by indirect immunofluorescence. The tissue cult...

journal_title：Microbiology and immunology

authors： Kondo Y,Morita Y,Yamada A,Kimura H

更新日期：2004-01-01 00:00:00

abstract：:The aim of this study was to explore the presence of methicillin-resistant Staphylococcus pseudintermedius (MRSP) in a collection of S. pseudintermedius strains isolated from dogs and cats with dermatitis in Japan and to compare their genotypic and phenotypic characteristics. Clonal relationships were determined by pu...

journal_title：Microbiology and immunology

authors： Bardiau M,Yamazaki K,Ote I,Misawa N,Mainil JG

更新日期：2013-07-01 00:00:00

abstract：:The in vitro effect of the heat-labile toxin (HLT) of Bordetella parapertussis on HeLa, baby hamster kidney (BHK), chinese hamster ovary (CHO), myeloma (Pa-NS-1), human embryonic lung (HEL-R66) cells, erythrocytes, adipocytes and lymphocytes from guinea pigs, mice or rats, or aortic smooth muscle cells from pigs or gu...

journal_title：Microbiology and immunology

authors： Endoh M,Nagai M,Ueda T,Yoshida Y,Nakase Y

更新日期：1988-01-01 00:00:00

abstract：:The incidence of infection by Actinobacillus actinomycetemcomitans, one of the important pathogens in human periodontal diseases, has been reported to be associated with racial background and genetic factors. We attempted to determine the genetic regulation of immune responses to A. actinomycetemcomitans fimbriae, an ...

journal_title：Microbiology and immunology

authors： Honma K,Ishii N,Kato T,Ishihara K,Okuda K,Okuda K

更新日期：1997-01-01 00:00:00

abstract：:SCG is a major 6-branched 1,3-beta-D-glucan in Sparassis crispa Fr. SCG shows antitumor activity and also enhances the hematopoietic response in cyclophosphamide (CY)-treated mice. In the present study, the molecular mechanism of the enhancement of the hematopoietic response was investigated. The levels of interferon-...

journal_title：Microbiology and immunology

authors： Harada T,Kawaminami H,Miura NN,Adachi Y,Nakajima M,Yadomae T,Ohno N

更新日期：2006-01-01 00:00:00

abstract：:The IFN-induced double-stranded RNA (dsRNA)-activated protein kinase PKR is one of the key molecules in the antiviral effects of IFN. To clarify the effects of hepatitis C virus nonstructural protein 5A (NS5A) on antiviral activity of IFN, in particular on PKR kinase activity, in mammalian cells, we established induci...

journal_title：Microbiology and immunology

authors： Noguchi T,Satoh S,Noshi T,Hatada E,Fukuda R,Kawai A,Ikeda S,Hijikata M,Shimotohno K

更新日期：2001-01-01 00:00:00

abstract：:We analyzed the mechanism of spontaneous B cell activation in lupus mice by using anticlass-II antibody in vitro. The in vitro culture of B cells from old NZB mice markedly produced Ig without any stimulation, while B cells from NZW mice did not. The addition of anticlass-II antibody (anti-Iad antibody) to the culture...

journal_title：Microbiology and immunology

authors： Saito K,Tanaka Y,Ota T,Eto S,Yamashita U

更新日期：1991-01-01 00:00:00

abstract：:Staphylococcal exfoliative toxin A (ETA) had a splitting effect at the granular layer of skin in humans and neonatal mice, but not in rabbits, guinea pigs, golden hamsters, or rats. Besides its splitting effect, ETA could stimulate productions of neutralizing antibody to ETA in rabbits, rats and B10D2 mice, but not in...

journal_title：Microbiology and immunology

authors： Machida K,Sakurai S,Kondo I,Ikawa S

更新日期：1988-01-01 00:00:00

abstract：:Rabies virus Nishigahara strain causes lethal infection in adult mice after intracerebral inoculation. On the other hand, the RC-HL strain, derived from the Nishigahara strain, does not cause lethal infection in adult mice. We previously demonstrated that a chimeric virus, R(G), with the open reading frame of the G ge...

journal_title：Microbiology and immunology

authors： Yamada K,Ito N,Takayama-Ito M,Sugiyama M,Minamoto N

更新日期：2006-01-01 00:00:00

abstract：:The Env and Gag proteins of HIV-1 are the two major structural proteins of this retrovirus. The interactions between Env and Gag proteins and their regulation in HIV-1 are required for several steps of the replication cycle, involving not only virus assembly, specifically Env incorporation, but also entry steps after ...

journal_title：Microbiology and immunology

authors： Murakami T

更新日期：2008-05-01 00:00:00

abstract：:A simple method for the separation and identification of Bacteroides fragilis from other Bacteroides species through the use of oxgall-impregnated filter paper disks is described. As in the antibiotic sensitivity test, filter paper disks containing defined amounts of bile salt were placed on a lawn of bacterial cells ...

journal_title：Microbiology and immunology

authors： Yamazaki E,Sugimoto K,Niwano K,Okada J

更新日期：1982-01-01 00:00:00

abstract：:The genus Enhydrobacter, first reported as a member of the family Vibrionaceae, has been placed in the family Moraxellaceae, but as a genus incertae sedis in Bergey's Manual of Systematic Bacteriology 2nd edition. During our taxonomic investigation of Enhydrobacter-like organisms, we observed that the 16S rRNA sequenc...

journal_title：Microbiology and immunology

authors： Kawamura Y,Fujiwara N,Naka T,Mitani A,Kubota H,Tomida J,Morita Y,Hitomi J

更新日期：2012-01-01 00:00:00

abstract：:Hg- and Cd-spores of Bacillus megaterium QM B1551 were produced in Schaeffer's medium containing mercuric chloride and cadmium chloride respectively. Metals were added to the medium at 9 hr of incubation (Stage V) to give a final concentration of 50 microM. It was found by electron microscopic and biochemical studies ...

journal_title：Microbiology and immunology

authors： Imagawa M,Ichikawa T,Kondo M

更新日期：1982-01-01 00:00:00

abstract：:The ompA gene, 981 bp in size and identified in the Mycobacterium bovis genome, was cloned and sequenced. Predicted amino acid sequences showed a protein of molecular weight 33.5 kDa with an isoelectric point of 7.28 and an OmpA conserved domain at the carboxy terminal with signal sequence at the N-terminal. Homology ...

journal_title：Microbiology and immunology

authors： Singh JP,Verma R

更新日期：2008-08-01 00:00:00

abstract：:Adult T-cell leukemia-associated membrane antigen (ATLMA) expressed on the surface of living ATL virus (ATLV)-carrying cells was investigated by an indirect membrane immunofluorescence method using natural antibodies to ATLV in human sera. All the ATLV-positive cell lines tested that had cytoplasmic ATL-associated ant...

journal_title：Microbiology and immunology

authors： Chosa T,Yamamoto N,Koyanagi Y,Kohno M,Nagata K,Hinuma Y

更新日期：1984-01-01 00:00:00

abstract：:The biological effect of purified beta toxin of Clostridium perfringens type C in vivo was investigated. After intravenous injection of the purified beta toxin into rats, a rise in blood pressure and a simultaneous fall in heart rate were observed. After the blood pressure reached a maximum, the heart rate recovered g...

journal_title：Microbiology and immunology

authors： Sakurai J,Fujii Y,Matsuura M,Endo K

更新日期：1981-01-01 00:00:00

abstract：:M protein is an important virulence determinant in Streptococcus pyogenes, but the amounts of M protein in various strains of the species remain to be elucidated. To assess the amount of M protein in strains of each emm genotype, dot blot analysis was performed on 141 clinically isolated strains. Among the cell membra...

journal_title：Microbiology and immunology

authors： Matsumoto M,Suzuki M,Hirose K,Hiramatsu R,Minagawa H,Minami M,Tatsuno I,Okamoto A,Ohta M,Hasegawa T

更新日期：2011-06-01 00:00:00

abstract：:Butanol-extracted water-soluble adjuvant (Bu-WSA) obtained from Bacterionema matruchotii was cultured with peripheral blood mononuclear cells (PBM) in the presence of sub- and/or supra-optimal mitogenic concentrations of concanavalin A (Con A). The addition of Bu-WSA resulted in increased tritiated thymidine incorpora...

journal_title：Microbiology and immunology

authors： Nitta T,Okumura S,Tsushi M,Nakano M

更新日期：1982-07-01 00:00:00

abstract：:Alpha-fetoprotein (AFP) expression is observed in embryonic tissues and, the expression of this protein is absent in normal adult tissues. The re-elevation of serum AFP strongly suggests generation of a malignant tumor in an adult. We demonstrated here that AFP-producing gastric cancer (AFP-gastric cancer) could be tr...

journal_title：Microbiology and immunology

authors： Miura Y,Kataoka H,Joh T,Tada T,Asai K,Nakanishi M,Okada N,Okada H

更新日期：2004-01-01 00:00:00

abstract：:To study the role of Moraxella (subgenus Branhamella) catarrhalis (B. catarrhalis) adherence to airway cells in lower respiratory tract infections, the in vitro attachments of B. catarrhalis to upper airway (oropharyngeal) and lower airway (bronchial) epithelial cells were compared. The adherence of 4 strains (1 nonfi...

journal_title：Microbiology and immunology

authors： Rikitomi N,Ahmed K,Nagatake T

更新日期：1997-01-01 00:00:00

abstract：:Congenitally athymic BALB/cA nu/nu mice were employed to elucidate the role of the thymus in experimental Mycoplasma pulmonis strain m53 infection, and nu/+ mice were used for comparison. Chronic polyarthritis was frequently produced in both of nu/nu and nu/+ mice by intravenous injection of the organisms. Macroscopic...

journal_title：Microbiology and immunology

authors： Tanaka H

更新日期：1979-01-01 00:00:00

abstract：:We determined the linkage of 16S, 23S, and 5S rRNA genes in several strains of Leptospira and Leptonema by DNA-DNA hybridization. Almost all the hybridizations in all leptospires used in these experiments gave two radioactive bands and the results strongly suggest that the number of the 16S and the 23S rRNA genes in t...

journal_title：Microbiology and immunology

authors： Fukunaga M,Masuzawa T,Okuzako N,Mifuchi I,Yanagihara Y

更新日期：1990-01-01 00:00:00

abstract：:In this review, we have emphasized: 1) bacterial lipopolysaccharide (LPS) involvement in IgA responses to orally administered thymic-dependent (TD) antigens; 2) characterization of Peyer's patch (PP) lymphoreticular cells; and 3) gastrointestinal immunization with gram negative pathogens and anti-LPS immunity to infec...

journal_title：Microbiology and immunology

authors： McGhee JR,Michalek SM,Kiyono H,Eldridge JH,Colwell DE,Williamson SI,Wannemuehler MJ,Jirillo E,Mosteller LM,Spalding DM

更新日期：1984-01-01 00:00:00

abstract：:Measuring the amount of HIV-1 DNA in infected cells is important to estimate the size of the viral reservoir in patients. However, the clinical impact of the intracellular viral DNA level remains unclear. The present study examines the clinical significance of the HIV-1 DNA level in peripheral CD4+ T lymphocytes from ...

journal_title：Microbiology and immunology

authors： Wada K,Nagai H,Hagiwara T,Ibe S,Utsumi M,Kaneda T

更新日期：2004-01-01 00:00:00

abstract：:Sodium vanadate enhanced Newcastle disease virus (NDV)-induced cell fusion in L cells, and there was a direct correlation between the degree of cell fusion and the dose of vanadate added. When anti-F protein of NDV monospecific antiserum was added to the culture fluid of L cells infected with NDV, the enhancement of c...

journal_title：Microbiology and immunology

authors： Yamamoto F,Fujioka H,Iinuma M,Takano M,Maeno K,Nagai Y,Ito Y

更新日期：1984-01-01 00:00:00

abstract：:We investigated here the kinetics of natural killer (NK) cells and extrathymic T cells, which include intermediate CD3 cells and gamma delta T cells, in the cord factor-induced granulomatous inflammation of the lungs and liver. In Balb/c mice, pulmonary inflammation elevated the proportion of NK cells and that of extr...

journal_title：Microbiology and immunology

authors： Tabata A,Kaneda K,Watanabe H,Abo T,Yano I

更新日期：1996-01-01 00:00:00

abstract：:Periapical lesions are induced by bacterial infection of the dental pulp and result in destruction of the surrounding alveolar bone. Although various immunological studies concerning periapical bone resorption have been reported, the role of cytokines in the formation of periapical lesions remains unclear. In this stu...

journal_title：Microbiology and immunology

authors： Oseko F,Yamamoto T,Akamatsu Y,Kanamura N,Iwakura Y,Imanishi J,Kita M

更新日期：2009-05-01 00:00:00

abstract：:Sequence analysis of the gene encoding the major neutralization glycoprotein (VP7) was performed on sixteen human isolates of serotype 2 of rotavirus in Japan, China, and Pakistan and their genetic variations were examined. Comparative studies of their nucleotide and deduced amino acid sequences between the sixteen is...

journal_title：Microbiology and immunology

authors： Wen L,Ushijima H,Kakizawa J,Fang ZY,Nishio O,Morikawa S,Motohiro T

更新日期：1995-01-01 00:00:00