Cytopathic effect of heat-labile toxin of Bordetella parapertussis on aortic smooth muscle cells from pigs or guinea pigs.

abstract：:Serum samples were collected from 938 pigs of 24 farms in Hokkaido, Kagoshima, and Okinawa prefectures in Japan in 2001-2005. Enzyme-linked immunosorbent assay (ELISA) was used for the detection of antibodies to LipL32 antigen which is common to Leptospira interrogans. Samples positive in ELISA were then investigated ...

journal_title：Microbiology and immunology

authors： Naito M,Sakoda Y,Kamikawa T,Nitta Y,Hirose K,Sakashita M,Kurokawa S,Kida H

更新日期：2007-01-01 00:00:00

abstract：:We investigated sex- and age-specific prevalences of hepatitis B e antigen (HBeAg) and antibody to HBeAg (anti-HBe) in 1,163 carriers of hepatitis B surface antigen (HBsAg) in Okinawa, Japan, and followed them up for longer than one year in correlation with liver function abnormalities. Elevated serum transaminase lev...

journal_title：Microbiology and immunology

authors： Hayashi J,Kashiwagi S,Ikematsu H,Nomura H,Kajiyama W,Noguchi A,Ikeda K

更新日期：1988-01-01 00:00:00

abstract：:Using a mouse monoclonal antibody (MAb) 2F raised against Vibrio cholerae non-O1 heat-stable enterotoxin (NAG-ST) which also recognizes a shared epitope of Yersinia enterocolitica heat-stable enterotoxin (Y-ST), a competitive enzyme-linked immunosorbent assay (ELISA) was developed for independent detection of NAG-ST a...

journal_title：Microbiology and immunology

authors： Nair GB,Bhattacharya SK,Takeda T

更新日期：1993-01-01 00:00:00

abstract：:In order to elucidate the molecular characteristics of Japanese encephalitis (JE) virus in Okinawa, 23 strains of JE virus isolated in a 25-year span were sequenced for the 240 nucleotides of the C-preM junction region and 111 nucleotides of the E gene region and compared with those of reference strains isolated in ma...

journal_title：Microbiology and immunology

authors： Ma SP,Arakaki S,Makino Y,Fukunaga T

更新日期：1996-01-01 00:00:00

abstract：:An oral delivery system based on ApxIIA#5-expressed on Saccharomyces cerevisiae was studied for its potential to induce immune responses in mice. Murine bone marrow-derived dendritic cells (DCs) stimulated in vitro with ApxIIA#5-expressed on S. cerevisiae upregulated the expression of maturation and activation markers...

journal_title：Microbiology and immunology

authors： Shin MK,Lee WJ,Jung MH,Cha SB,Shin SW,Yoo A,Kim DH,Yoo HS

更新日期：2013-06-01 00:00:00

abstract：:Clostridium perfringens KZ1340 isolated from Antarctic soil was first classified as Clostridium plagarum and later as a lecithinase-negative variant of C. perfringens. Although the strain produced no detectable lecithinase (phospholipase C, PLC) activity in the culture supernatant, it was shown by Southern blot hybrid...

journal_title：Microbiology and immunology

authors： Kameyama K,Matsushita O,Katayama S,Minami J,Maeda M,Nakamura S,Okabe A

更新日期：1996-01-01 00:00:00

abstract：:Hemadsorption (HAD) induced in HEp-2 cells infected with vaccinia virus was observed. In ultrathin sections, binding of 36 red blood cells (RBCs) was examined in detail and 3 types of HAD were observed: (1) direct and close binding of RBCs to infected HEp-2 cells (cyto-HAD) was observed in cross sections of 27 RBCs (2...

journal_title：Microbiology and immunology

authors： Saburi Y,Okuda K,Takahashi T,Tadokoro I

更新日期：1977-10-20 00:00:00

abstract：:The killing activity of cord blood mononuclear cells (cMNC) against cytomegalovirus (CMV)-uninfected and -infected fibroblasts was comparable to that of adult peripheral blood mononuclear cells (aPBMC). The killing activity of cMNC against K562 cells was significantly lower compared with that of aPBMC. Treatment of cM...

journal_title：Microbiology and immunology

authors： Hoshina T,Kida K,Ito M

更新日期：1999-01-01 00:00:00

abstract：:By subcutaneous inoculation of 10(8) CFU of enterohemorrhagic E. coli O157:H7, specific-pathogen-free mice revealed most of the symptoms and histological changes observed in patients. The histological changes in intestine were mainly seen in the distal parts of small intestine and the cecum. Vacuolation of villi in th...

journal_title：Microbiology and immunology

authors： Lai XH,Xu JG,Liu BY

更新日期：1991-01-01 00:00:00

abstract：:The effects of recombinant human interleukin-2 (rIL-2) administered prophylactically on the death of CBA/J mice challenged with Klebsiella pneumoniae 27 intraperitoneally were examined. rIL-2 administered subcutaneously at 20 micrograms per mouse for 7 days enhanced survival after a lethal challenge. The injection of ...

journal_title：Microbiology and immunology

authors： Iizawa Y,Nakao M,Kondo M,Yamazaki T

更新日期：1990-01-01 00:00:00

abstract：:Burkholderia pseudomallei, a pathogenic gram-negative bacterium, causes the severe human disease melioidosis. This organism can survive in eukaryotic host cells by escaping reactive oxygen species via the regulation of stress responsive sigma factors, including RpoS. In B. pseudomallei, RpoS has been reported to play ...

journal_title：Microbiology and immunology

authors： Chutoam P,Charoensawan V,Wongtrakoongate P,Kum-Arth A,Buphamalai P,Tungpradabkul S

更新日期：2013-09-01 00:00:00

abstract：:One strain each of granular and downy varieties of Trichophyton mentagrophytes and two strains each of granular and floccose colonial types Trichophyton rubrum were used for preparation of antigens. The antigens were analyzed by double diffusion against homologous and heterologous antisera raised in rabbits. A total o...

journal_title：Microbiology and immunology

authors： Shukla NP,Agarwal GP

更新日期：1983-01-01 00:00:00

abstract：:We prepared polyclonal antibody specific to Mycoplasma pneumoniae. Using this antibody, we developed a latex agglutination test (LAT) for detecting the organism in respiratory exudates as rapid diagnosis of M. pneumoniae infection. Further, LAT was compared with DNA-probe test (DP) which was the only commercially avai...

journal_title：Microbiology and immunology

authors： Masayoshi T,Hirai Y,Kanemasa Y

更新日期：1992-01-01 00:00:00

abstract：:Murine γδ T cells develop as the first T-cell lineage within the fetal thymus and disproportionately localize in mucosal tissues such as lung, skin, uterus, and intestine of adult mice. These unique developmental features and distribution patterns of γδ T cells enable rapid functioning against various insults from pat...

journal_title：Microbiology and immunology

authors： Shibata K

更新日期：2012-04-01 00:00:00

abstract：:Samples of feces and vomit collected from patients during 13 non-bacterial gastroenteritis outbreaks which occurred in Hokkaido between 1995 and 1998 were examined by electron microscopy (EM) and reverse-transcription polymerase chain reaction (RT-PCR) for evidence of infection with human caliciviruses (HuCVs). In 6 f...

journal_title：Microbiology and immunology

authors： Ohyama T,Yoshizumi S,Sawada H,Uchiyama Y,Katoh Y,Hamaoka N,Utagawa E

更新日期：1999-01-01 00:00:00

abstract：:Antibodies to influenza A virus were detected using enzyme-linked immunosorbent assay (ELISA) in the sera from two of seven Baikal seals (Phoca sibrica) and from five of six ringed seals (Phoca hispida) in Russia. In a hemagglutination-inhibition test using H1-H15 reference influenza A viruses, ELISA-positive sera fro...

journal_title：Microbiology and immunology

authors： Ohishi K,Kishida N,Ninomiya A,Kida H,Takada Y,Miyazaki N,Boltunov AN,Maruyama T

更新日期：2004-01-01 00:00:00

abstract：:Streptococcus suis infection induces formation of neutrophil extracellular traps (NETs) in vitro; however, the contribution of NETs-mediated killing to the pathogenesis of S. suis in vivo is yet to be elicited. The findings of the present study indicated that extracellular DNA fiber can be induced in a murine model in...

journal_title：Microbiology and immunology

authors： Zhao J,Lin L,Fu L,Han L,Zhang A

更新日期：2016-04-01 00:00:00

abstract：:The current vaccination strategy against influenza is to induce production of antibodies directed against the surface antigens of these viruses. However, frequent changes in the surface antigens of influenza viruses allow them to avoid antibody-mediated immunity. On the other hand, it is known that cytotoxic T-lymphoc...

journal_title：Microbiology and immunology

authors： Uchida T

更新日期：2011-01-01 00:00:00

abstract：:Frauch's micro-slide method was improved to facilitate the endpoint-determination of the Limulus test. Two precise observations, by inverted phase contrast microscopy and with a staining procedure, were newly performed as additions to the slide test. The staining procedure was proposed as an improved method for the Li...

journal_title：Microbiology and immunology

authors： Okuguchi S

更新日期：1978-01-01 00:00:00

abstract：:B lymphocytes express multiple TLRs that regulate their cytokine production.We investigated the effect of TLR4 and TLR9 activation on receptor activator of NF‐kB ligand (RANKL) expression by rat spleen B cells. Splenocytes or purified spleen B cells from Rowett rats were cultured with TLR4 ligand Escherichia coli LPS ...

journal_title：Microbiology and immunology

authors： Yu X,Lin J,Yu Q,Kawai T,Taubman MA,Han X

更新日期：2014-01-01 00:00:00

abstract：:Intracellular localization of the wild-type (Spv+), the phoP-constitutively expressed strain (PhoPc), and the spv-deleted strain (Spv-) of Salmonella typhimurium was examined by the use of confocal laser scanning microscopy analysis of immunostained sections of mouse spleens after oral or subcutaneous inoculation. Onl...

journal_title：Microbiology and immunology

authors： Matsui H,Eguchi M,Kikuchi Y

更新日期：2001-01-01 00:00:00

abstract：:Measles vaccines are highly effective and safe; however, the mechanism(s) underlying their attenuation has not been well understood. In this study, type I IFNs (IFN-α and IFN-β) induction in macaques infected with measles virus (MV) strains was examined. Type I IFNs were not induced in macaques infected with wild-type...

journal_title：Microbiology and immunology

authors： Van Nguyen N,Kato SI,Nagata K,Takeuchi K

更新日期：2016-07-01 00:00:00

abstract：:We have developed an indirect microhemagglutination test (IMHA) for detecting antibodies to Borrelia burgdorferi using formalin-fixed chicken erythrocytes sensitized with a sonicated extract of the bacteria. The specificity of IMHA for Lyme disease was compared with that of a conventional indirect immunoperoxidase tec...

journal_title：Microbiology and immunology

authors： Fujita H,Sato T,Ohara Y,Homma M

更新日期：1991-01-01 00:00:00

abstract：:Opaque (Op) and translucent (Tr) colonial variants were isolated from Vibrio vulnificus strains. Op-type variants were more resistant than the isogenic Tr-type variants, but the survival rate of the Op-type variants varied with the strains. Antisera were prepared by immunizing rabbit with whole cells of Op and Tr vari...

journal_title：Microbiology and immunology

authors： Shinoda S,Kobayashi M,Yamada H,Yoshida S,Ogawa M,Mizuguchi Y

更新日期：1987-01-01 00:00:00

abstract：:Germfree (GF) F344 rats developed severe adjuvant-induced arthritis with a 100% incidence after a single intradermal injection of heat-killed Mycobacterium bovis (BCG). Specific pathogene-free (SPF) rats developed less severe arthritis with a lower incidence. The rats colonized with Escherichia coli or Bacteroides dev...

journal_title：Microbiology and immunology

authors： Kohashi O,Kohashi Y,Takahashi T,Ozawa A,Shigematsu N

更新日期：1985-01-01 00:00:00

abstract：:Bifidobacteria are important members of the intestinal microbiota and are considered to contribute to maintaining health. However, the level of bifidobacteria colonising the intestine of elderly subjects tends to be lower than in younger adults. Therefore, two Bifidobacterium longum strains, isolated from healthy elde...

journal_title：Microbiology and immunology

authors： Mäkeläinen H,Tahvonen R,Salminen S,Ouwehand AC

更新日期：2003-01-01 00:00:00

abstract：:To investigate cellular components incorporated into the rabies virion, monoclonal antibodies (MAbs) were screened based on their reactivity with additional virion components. Two of the MAbs we prepared recognized a virion-associated 21 kDa polypeptide (referred to as VAP21) from a BHK-21 cell. Since the MAbs precipi...

journal_title：Microbiology and immunology

authors： Sagara J,Tochikura TS,Yamamoto T,Tsukita S,Tsukita S,Kawai A

更新日期：1997-01-01 00:00:00

abstract：:A possible role of intracellular Ca2+ and participation of calmodulin in cellular metabolism in endotoxin-poisoned mice were investigated. The levels of calcium in liver cytosol and liver mitochondria fractions in poisoned mice were markedly higher 18-48 hr after endotoxin injection than in the control mice. On the ot...

journal_title：Microbiology and immunology

authors： Sakaguchi S,Abe H,Sakaguchi O

更新日期：1984-01-01 00:00:00

abstract：:The 16S toxin was purified from a Clostridium botulinum type D strain 1873 (D-1873). Furthermore, the entire nucleotide sequences of the genes coding for the 16S toxin were determined. It became clear that the purified D-1873 16S toxin consists of neurotoxin, nontoxic nonhemagglutinin (NTNH), and hemagglutinin (HA), a...

journal_title：Microbiology and immunology

authors： Nakajima H,Inoue K,Ikeda T,Fujinaga Y,Sunagawa H,Takeshi K,Ohyama T,Watanabe T,Inoue K,Oguma K

更新日期：1998-01-01 00:00:00

abstract：:Polar monotrichous flagella (M-flagella) of Vibrio parahaemolyticus have antigens in common with those of various species of Vibrio including V. cholerae and V. anguillarum, and of Beneckea, revealed by gel diffusion tests with flagelli monomers. However, antiserum against M-flagellin of V. parahaemolyticus did not ag...

journal_title：Microbiology and immunology

authors： Shinoda S,Senoh T,Asano K,Nakahara N,Ono B

更新日期：1980-01-01 00:00:00