Immunological studies of a 21 kDa cellular component efficiently incorporated into rabies virion grown in a BHK-21 cell culture.

abstract：:Twelve hybridoma cell lines producing monoclonal antibodies to the human interleukin 2 (IL-2) receptor (IL-2R) molecule were prepared. These antibodies were characterized by competitive antibody-binding assay and sequential immunoprecipitation assay with four known monoclonal antibodies to the human IL-2R molecule. Th...

journal_title：Microbiology and immunology

authors： Tanaka Y,Inoi T,Tozawa H,Sugamura K,Hinuma Y

更新日期：1986-01-01 00:00:00

abstract：:Previous studies have demonstrated that the murine monoclonal antibody (MoAb) NM-01 activates the human complement classical pathway resulting in lysis of human immunodeficiency virus (HIV). The present study was performed to determine the availability of the V3-loop of gp120 relative to the complement regulatory prot...

journal_title：Microbiology and immunology

authors： Nakamura M,Okada H,Sasaki H,Yoshida K,Kamada M,Okada N,Terada M,Ohno T

更新日期：1996-01-01 00:00:00

abstract：:The present study was done to determine the molecular epidemiology of rabies virus (RV) in Vietnam. The nucleoprotein (N) and glycoprotein (G) genes of RVs were amplified from the brains of ten rabid dogs of Ho Chi Minh City, Vietnam. The nucleotide sequences of these genes were compared with those of other Asian stra...

journal_title：Microbiology and immunology

authors： Yamagata J,Ahmed K,Khawplod P,Mannen K,Xuyen DK,Loi HH,Dung NV,Nishizono A

更新日期：2007-01-01 00:00:00

abstract：:A human T-lymphoblastoid cell line, TCL-Fuj, produces large amounts of interferon (IFN)-gamma constitutively. A variant cell line, 2M, was derived from it. Both cell lines express similar surface antigen markers, but differ in surface morphology. Compared with the parent TCL-Fuj cell line, 2M produced less IFN-gamma c...

journal_title：Microbiology and immunology

authors： Matsuyama M,Sugamura K,Hinuma Y

更新日期：1987-01-01 00:00:00

abstract：:Absorption of a pooled human gamma globulin preparation with acetone-treated measles virus-infected cells removed all antibodies to measles virus antigens except a portion of the antibody to the fusion (F) protein. The residual anti-F antibody had hemolysis-inhibiting and virus-neutralizing activities, inhibited sprea...

journal_title：Microbiology and immunology

authors： Sato TA,Kohama T,Sugiura A

更新日期：1989-01-01 00:00:00

abstract：:Macrophages act as a reservoir for Mycobacterium tuberculosis, producing latent infection in approximately 90% of infected people. In this study, J774A.1 mouse macrophage cell line response and microRNA (miRNA) expression during infection with the most relevant mycobacterial strains for humans (M. tuberculosis, M. bov...

journal_title：Microbiology and immunology

authors： Elizabeth MC,Hernández de la Cruz ON,Mauricio CA

更新日期：2016-05-01 00:00:00

abstract：:An oral delivery system based on ApxIIA#5-expressed on Saccharomyces cerevisiae was studied for its potential to induce immune responses in mice. Murine bone marrow-derived dendritic cells (DCs) stimulated in vitro with ApxIIA#5-expressed on S. cerevisiae upregulated the expression of maturation and activation markers...

journal_title：Microbiology and immunology

authors： Shin MK,Lee WJ,Jung MH,Cha SB,Shin SW,Yoo A,Kim DH,Yoo HS

更新日期：2013-06-01 00:00:00

abstract：:Although the RC-HL strain of rabies virus is avirulent in adult mice, the amino acid at position 333 of its G protein is arginine, which is thought to be necessary for virulence in adult mice upon intracerebral inoculation of the virus. This result suggests that besides arginine at position 333, some other positions o...

journal_title：Microbiology and immunology

authors： Ito H,Minamoto N,Watanabe T,Goto H,Rong LT,Sugiyama M,Kinjo T,Mannen K,Mifune K,Konobe T

更新日期：1994-01-01 00:00:00

abstract：:Murine B cell mitogens such as bacterial lipopolysaccharide (LPS), butanol-extracted water soluble adjuvant (Bu-WSA), dextran sulfate (DS), synthetic muramyl dipeptide (MDP), and its analog MDP-Lys (L18) do not show any mitogenic ability in vitro on human peripheral blood lymphocytes or mixed cell populations of purif...

journal_title：Microbiology and immunology

authors： Nitta T,Konno-Ejiri H,Okumura S,Ozawa A,Nakano M

更新日期：1985-01-01 00:00:00

abstract：:We previously reported the establishment of an HEp2 cell line which expresses the US3 protein kinase (PK) of herpes simplex virus type 2 (HSV-2) upon induction with IPTG. Here we report that expression, phosphorylation and ubiquitination of cytokeratin 17 (CK17) are enhanced in US3-expressing HEp2 cells. In vitro kina...

journal_title：Microbiology and immunology

authors： Murata T,Goshima F,Nishizawa Y,Daikoku T,Takakuwa H,Ohtsuka K,Yoshikawa T,Nishiyama Y

更新日期：2002-01-01 00:00:00

abstract：:We found that a sub-lethal concentration of hydrogen peroxide (HPOx) enhanced the growth of Helicobacter pylori in Brucella broth supplemented with 10% fetal bovine serum (BB/FBS). The enhancement was evident at 0.1 mM HPOx and reached a maximun at 3.5 mM. The growth stimulation was dependent on the basal media used; ...

journal_title：Microbiology and immunology

authors： Murano A,Miyake M,Kato J,Tanzawa H,Takeo K,Noda M

更新日期：1999-01-01 00:00:00

abstract：:We observed the effects of a chinese herb medicine Sho-saiko-to on the lethal and antitumor activities of recombinant human tumor necrosis factor (rhTNF) administered in mice. Sho-saiko-to was noted to protect the rhTNF-induced lethality in galactosamine-hypersensitized mice, and also Sho-saiko-to pretreated mice was ...

journal_title：Microbiology and immunology

authors： Sakaguchi S,Tutumi E,Yokota K,Furusawa S,Sasaki K,Takayanagi Y

更新日期：1991-01-01 00:00:00

abstract：:The fusion of BHK-21-KB cells by vesicular stomatitis virus was not induced in Eagle's minimal essential medium without glucose. In medium containing glucose, the rate of polykaryocyte formation decreased as the concentration of glucose was reduced below 5 mM. However, no reduction in virus production 24 hr after infe...

journal_title：Microbiology and immunology

authors： Takehara M

更新日期：1981-01-01 00:00:00

abstract：:There are many examples of probiotic effects of various lactic bacteria on enteropathogens. In this study, Lactobacillus strains (L. rhamnosus, L. gasseri, L. casei and L. plantarum) were tested in an in vitro model of enterohemorrhagic Escherichia coli (EHEC) infection of a human colon epithelial cell line, C2BBe1. W...

journal_title：Microbiology and immunology

authors： Hirano J,Yoshida T,Sugiyama T,Koide N,Mori I,Yokochi T

更新日期：2003-01-01 00:00:00

abstract：:The genus Enhydrobacter, first reported as a member of the family Vibrionaceae, has been placed in the family Moraxellaceae, but as a genus incertae sedis in Bergey's Manual of Systematic Bacteriology 2nd edition. During our taxonomic investigation of Enhydrobacter-like organisms, we observed that the 16S rRNA sequenc...

journal_title：Microbiology and immunology

authors： Kawamura Y,Fujiwara N,Naka T,Mitani A,Kubota H,Tomida J,Morita Y,Hitomi J

更新日期：2012-01-01 00:00:00

abstract：:Patients with typhoid fever presenting to the Tokyo Metropolitan Komagome Hospital during the period 1975-1998 were retrospectively investigated. All cases were diagnosed by a positive culture for Salmonella typhi in either of their clinical specimens. Of the total number of 130 patients, 57% contracted the disease ab...

journal_title：Microbiology and immunology

authors： Hoshino Y,Masuda G,Negishi M,Ajisawa A,Imamura A,Hachimori K,Takayama N,Yamaguchi T,Kimura M

更新日期：2000-01-01 00:00:00

abstract：:We determined the effect of heat-stable enterotoxin produced by Yersinia enterocolitica (Y. enterocolitica ST) on cyclic nucleotide levels in the intestines of 6-day-old mice and in cultured cell line cells. The concentration of cyclic guanosine 3',5'-monophosphate (cyclic GMP) in homogenates of the intestines increas...

journal_title：Microbiology and immunology

authors： Inoue T,Okamoto K,Moriyama T,Takahashi T,Shimizu K,Miyama A

更新日期：1983-01-01 00:00:00

abstract：:The standard products of V(D)J recombination of immunoglobulin and T cell receptor genes are two kinds of DNA junction, a coding joint and a signal joint. TCR delta V-D and D-D signal joints in adult mouse thymocytes were sequenced following PCR amplification. We observed differential nucleotide insertions at the V de...

journal_title：Microbiology and immunology

authors： Kanari Y,Muto M,Yamagishi H

更新日期：2003-01-01 00:00:00

abstract：:Polar monotrichous flagella (M-flagella) of Vibrio parahaemolyticus have antigens in common with those of various species of Vibrio including V. cholerae and V. anguillarum, and of Beneckea, revealed by gel diffusion tests with flagelli monomers. However, antiserum against M-flagellin of V. parahaemolyticus did not ag...

journal_title：Microbiology and immunology

authors： Shinoda S,Senoh T,Asano K,Nakahara N,Ono B

更新日期：1980-01-01 00:00:00

abstract：:A simple method for the separation and identification of Bacteroides fragilis from other Bacteroides species through the use of oxgall-impregnated filter paper disks is described. As in the antibiotic sensitivity test, filter paper disks containing defined amounts of bile salt were placed on a lawn of bacterial cells ...

journal_title：Microbiology and immunology

authors： Yamazaki E,Sugimoto K,Niwano K,Okada J

更新日期：1982-01-01 00:00:00

abstract：:Fcgamma receptor (R) IIB, a low-affinity FcR for IgG, inhibits B cell Ag R (BCR)-mediated activation when these two receptors are cross-linked by Ag and IgG-containing immune complexs (ICs). We found deletion polymorphisms in the promoter region of fcgr2b in NC/Nga mice, a model for human atopic dermatitis. NC/Nga mic...

journal_title：Microbiology and immunology

authors： Sakai T,Kogiso M,Mitsuya K,Komatsu T,Yamamoto S

更新日期：2006-01-01 00:00:00

abstract：:Alkaline phosphatase-conjugated oligonucleotide probes were developed to detect the gene coding for Vero toxin 1 (VT1) and Vero toxin 2 (VT2). Using these probes, 3 hr was enough to detect VT genes when suspicious colonies of enterohaemorrhagic Escherichia coli (EHEC) were obtained on an agar plate. The results of a h...

journal_title：Microbiology and immunology

authors： Yoh M,Takagi K,Eda J,Ohtomo M,Takarada Y,Shibata S,Honda T

更新日期：1997-01-01 00:00:00

abstract：:Samples of feces and vomit collected from patients during 13 non-bacterial gastroenteritis outbreaks which occurred in Hokkaido between 1995 and 1998 were examined by electron microscopy (EM) and reverse-transcription polymerase chain reaction (RT-PCR) for evidence of infection with human caliciviruses (HuCVs). In 6 f...

journal_title：Microbiology and immunology

authors： Ohyama T,Yoshizumi S,Sawada H,Uchiyama Y,Katoh Y,Hamaoka N,Utagawa E

更新日期：1999-01-01 00:00:00

abstract：:Frauch's micro-slide method was improved to facilitate the endpoint-determination of the Limulus test. Two precise observations, by inverted phase contrast microscopy and with a staining procedure, were newly performed as additions to the slide test. The staining procedure was proposed as an improved method for the Li...

journal_title：Microbiology and immunology

authors： Okuguchi S

更新日期：1978-01-01 00:00:00

abstract：:Leptospira was isolated from environmental water in central Japan using selective medium comprising five antibiotics, namely sulfamethoxazole, trimethoprim, amphotericin B, fosfomycin, and 5-fluorouracil. Of 100 water samples 57 (57%) were culture-positive and 50 pure cultures were isolated. Of the 50 cultures isolate...

journal_title：Microbiology and immunology

authors： Masuzawa T,Uno R,Matsuhashi N,Yamaguchi M,Xu J,Nakamura S

更新日期：2019-11-01 00:00:00

abstract：:Biofilm formation is an important phenotype associated with chronic Pseudomonas aeruginosa infections. In the present study, a total of 48 P. aeruginosa strains isolated from clinical specimens were examined for their biofilm-forming ability using a microtiter plate method. The different biofilm-forming abilities were...

journal_title：Microbiology and immunology

authors： Yang W,Shi L,Jia WX,Yin X,Su JY,Kou Y,Yi X,Shinoda S,Miyoshi S

更新日期：2005-01-01 00:00:00

abstract：:Burkholderia pseudomallei, a pathogenic gram-negative bacterium, causes the severe human disease melioidosis. This organism can survive in eukaryotic host cells by escaping reactive oxygen species via the regulation of stress responsive sigma factors, including RpoS. In B. pseudomallei, RpoS has been reported to play ...

journal_title：Microbiology and immunology

authors： Chutoam P,Charoensawan V,Wongtrakoongate P,Kum-Arth A,Buphamalai P,Tungpradabkul S

更新日期：2013-09-01 00:00:00

abstract：:Tetracycline (pp-tet), and kanamycin (pp-kan) resistance genes were cloned from a transferable R plasmid of fish pathogen Pasteurella piscicida, and complete nucleotide sequences were determined. The pp-tet was a class D Tet determinant constructed with the tetA resistance gene of 1,182 bp encoding a protein with a de...

journal_title：Microbiology and immunology

authors： Kim EH,Aoki T

更新日期：1994-01-01 00:00:00

abstract：:The mitogen effect on migration of eosinophils and monocytes was studied in embryonic chickens. On the 13th embryonic day, chickens were injected with mitogens, such as concanavalin A (Con A), phytohemagglutinin-P (PHA-P), and lipopolysaccharide (LPS), into the allantoic cavity, and the mitogenic effect was estimated ...

journal_title：Microbiology and immunology

authors： Moriya O,Ichikawa Y

更新日期：1979-01-01 00:00:00

abstract：:The in vitro activity of ofloxacin, a new pyridone-carboxylic acid, against 11 strains of Chlamydia trachomatis and six strains of Chlamydia psittaci was determined. All test strains of both species were inhibited by 0.39 micrograms of ofloxacin per ml. The antichlamydial activity of ofloxacin was comparable to that o...

journal_title：Microbiology and immunology

authors： Hirai K,Une T

更新日期：1986-01-01 00:00:00