Identification of heat-stable enterotoxin-producing strains of Yersinia enterocolitica and Vibrio cholerae non-O1 by a monoclonal antibody-based enzyme-linked immunosorbent assay.

Abstract:

:Using a mouse monoclonal antibody (MAb) 2F raised against Vibrio cholerae non-O1 heat-stable enterotoxin (NAG-ST) which also recognizes a shared epitope of Yersinia enterocolitica heat-stable enterotoxin (Y-ST), a competitive enzyme-linked immunosorbent assay (ELISA) was developed for independent detection of NAG-ST and Y-ST. There was good concordance between the Y-ST ELISA and the suckling mouse assay (SMA) for detection of Y-ST from test strains of Y. enterocolitica, and the Y-ST ELISA can effectively replace the SMA for routine detection of Y-ST. On the contrary, evaluation of the NAG-ST ELISA and the SMA using 139 strains of V. cholerae non-O1 showed discordant results and this was attributed to the presence of the suckling mice active factor(s) such as El Tor hemolysin and to the production of low amounts of NAG-ST. Concentration of culture supernatants of V. cholerae non-O1 followed by heating at 100 C was essential to obtain reproducible results by both the NAG-ST ELISA and the SMA. The ELISA developed in this study can be used for the identification of biologically active strains. While recently genetic methods such as polymerase chain reaction became available and were very reliable and simple techniques, the ELISA in this study has an advantage in detecting biologically toxic gene products of the strains. The genetic methods cannot differentiate silent STa genes which we often encounter in the case of Y. enterocolitica.

journal_name

Microbiol Immunol

authors

Nair GB,Bhattacharya SK,Takeda T

doi

10.1111/j.1348-0421.1993.tb03198.x

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

181-6

issue

3

eissn

0385-5600

issn

1348-0421

journal_volume

37

pub_type

杂志文章
  • Effects of cytochalasins B and D on Staphylococcus aureus adherence to and ingestion by mouse renal cells from primary culture.

    abstract::Cytochalasin B (CB) and cytochalasin D (CD), inhibitors of microfilament function of host cell, were examined for their effects on Staphylococcus aureus Cowan I adherence to and ingestion by several types of the hyperosmolarity-tolerant (HOT) cells obtained from primary culture of mouse kidney. Staphylococcal adherenc...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1993.tb03181.x

    authors: Murai M,Seki K,Sakurada J,Usui A,Masuda S

    更新日期:1993-01-01 00:00:00

  • Characterization of antibody response to Pseudomonas aeruginosa in patients with wound infections.

    abstract::ELISA was used to measure the amount and avidity of IgG antibodies to exotoxin A (ExA) and 7 Fisher's immunotypes of lipopolysaccharide (LPS) in the sera of 13 patients with mild or moderate Pseudomonas aeruginosa infections. Changes in the specificity of tested sera during the course of infection were demonstrated. A...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1991.tb01574.x

    authors: Trafny EA,Grzybowski J,Patzer J,Popiel D,Wrembel-Wargocka J,Dzierzanowska D,Zawistowska-Marciniak I,Kobus K

    更新日期:1991-01-01 00:00:00

  • Antibodies to human-related H3 influenza A virus in Baikal seals (Phoca sibirica) and ringed seals (Phoca hispida) in Russia.

    abstract::Antibodies to influenza A virus were detected using enzyme-linked immunosorbent assay (ELISA) in the sera from two of seven Baikal seals (Phoca sibrica) and from five of six ringed seals (Phoca hispida) in Russia. In a hemagglutination-inhibition test using H1-H15 reference influenza A viruses, ELISA-positive sera fro...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2004.tb03610.x

    authors: Ohishi K,Kishida N,Ninomiya A,Kida H,Takada Y,Miyazaki N,Boltunov AN,Maruyama T

    更新日期:2004-01-01 00:00:00

  • Suppressive effect of topoisomerase inhibitors on JC polyomavirus propagation in human neuroblastoma cells.

    abstract::JC polyomavirus (JCPyV) causes progressive multifocal leukoencephalopathy (PML), a fatal demyelinating disease of the central nervous system, in immunocompromised patients. Because no drugs have been approved for treating PML, many antiviral agents are currently being investigated for this purpose. The inhibitory effe...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12372

    authors: Nukuzuma S,Nakamichi K,Kameoka M,Sugiura S,Nukuzuma C,Tasaki T,Takegami T

    更新日期:2016-04-01 00:00:00

  • The thermostable direct hemolysin gene (tdh) of Vibrio hollisae is dissimilar in prevalence to and phylogenetically distant from the tdh genes of other vibrios: implications in the horizontal transfer of the tdh gene.

    abstract::Vibrio hollisae strains isolated recently from patients in various locations were examined for the presence of the thermostable direct hemolysin gene (tdh) using nucleic acid hybridization and polymerase chain reaction assays. The results were consistent with the previous finding that all strains of V. hollisae carry ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1996.tb03304.x

    authors: Nishibuchi M,Janda JM,Ezaki T

    更新日期:1996-01-01 00:00:00

  • A novel combination of selective agents for isolation of Leptospira species.

    abstract::A novel combination of antimicrobial agents (sulfamethoxazole, 40 μg/mL; trimethoprim, 20 μg/mL; amphotericin B, 5 μg/mL; fosfomycin, 400 μg/mL; and 5-fluorouracil, 100 μg/mL) was developed for selective isolation of leptospires from contaminated samples. The growth of 16 microorganisms considered as possible contamin...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2011.00347.x

    authors: Chakraborty A,Miyahara S,Villanueva SY,Saito M,Gloriani NG,Yoshida S

    更新日期:2011-07-01 00:00:00

  • Variation in virulence of Coxsackie virus B3 in the hearts of mice. I. Comparison of mortality and virus growth in the heart and other organs.

    abstract::Virulence of Coxsackie virus B3 (CB3) was compared in mice between strain SK-74 isolated from a patient and strain T-70 isolated from a healthy child as well as between prototype strain Nancy and its mouse-passaged derivative strain PMH. Strain SK-74 showed a high mortality (40-80%), while strain T-70 did not induce d...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1983.tb03588.x

    authors: Komatsu T,Hashimoto I,Kohara T

    更新日期:1983-01-01 00:00:00

  • In vivo safety assessment of two Bifidobacterium longum strains.

    abstract::Bifidobacteria are important members of the intestinal microbiota and are considered to contribute to maintaining health. However, the level of bifidobacteria colonising the intestine of elderly subjects tends to be lower than in younger adults. Therefore, two Bifidobacterium longum strains, isolated from healthy elde...

    journal_title:Microbiology and immunology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1111/j.1348-0421.2003.tb03464.x

    authors: Mäkeläinen H,Tahvonen R,Salminen S,Ouwehand AC

    更新日期:2003-01-01 00:00:00

  • Purification and characterization of heat-labile toxin from Bordetella bronchiseptica.

    abstract::The heat-labile toxin (HLT) of Bordetella bronchiseptica was purified successively from sonic extracts of phase I organisms grown in Stainer-Scholte medium, by partition in hydrophobic interaction, sucrose density gradient centrifugation, gel filtration through Sepharose 4B and 6B, isoelectric precipitation and isoele...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1986.tb02992.x

    authors: Endoh M,Amitani M,Nakase Y

    更新日期:1986-01-01 00:00:00

  • Mycobacterial cord factor enhances migration of neutrophil-like HL-60 cells by prolonging AKT phosphorylation.

    abstract::Trehalose 6,6'-dimycolate (TDM), or cord factor, is a crucial stimulus of immune responses during Mycobacterium tuberculosis infection. Although TDM has immuno-stimulatory properties, including adjuvant activity and the ability to induce granuloma formation, the mechanisms underlying these remain unknown. We hypothesi...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12544

    authors: Lee WB,Yan JJ,Kang JS,Chung S,Kim LK

    更新日期:2017-12-01 00:00:00

  • Menadione-catalyzed O2- production by Escherichia coli cells: application of rapid chemiluminescent assay to antimicrobial susceptibility testing.

    abstract::This study proposes a novel chemiluminescent assay of bacterial activity. Luminol chemiluminescence (LC) was amplified on addition of menadione to Escherichia coli suspension, and it was effectively inhibited by addition of superoxide dismutase rather than catalase. This fact suggests that H2O2 produced from O2 by sup...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2001.tb02628.x

    authors: Yamashoji S,Manome I,Ikedo M

    更新日期:2001-01-01 00:00:00

  • Genetic variation in VP7 gene of human rotavirus serotype 2 (G2 type) isolated in Japan, China, and Pakistan.

    abstract::Sequence analysis of the gene encoding the major neutralization glycoprotein (VP7) was performed on sixteen human isolates of serotype 2 of rotavirus in Japan, China, and Pakistan and their genetic variations were examined. Comparative studies of their nucleotide and deduced amino acid sequences between the sixteen is...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1995.tb03277.x

    authors: Wen L,Ushijima H,Kakizawa J,Fang ZY,Nishio O,Morikawa S,Motohiro T

    更新日期:1995-01-01 00:00:00

  • Expression of interferon-stimulated gene 20 in vascular endothelial cells.

    abstract::ISG20 is an ribonuclease specific for single-stranded RNA and considered to play a role in innate immunity against virus infections. We herein show that both poly IC, an authentic double-stranded RNA, and IFN-gamma induced ISG20 expression in cultured HUVEC. Poly IC-induced ISG20 expression was inhibited by LY294002, ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2008.00004.x

    authors: Imaizumi T,Mechti N,Matsumiya T,Sakaki H,Kubota K,Yoshida H,Kimura H,Satoh K

    更新日期:2008-01-01 00:00:00

  • Molecular epidemiology of rabies in Vietnam.

    abstract::The present study was done to determine the molecular epidemiology of rabies virus (RV) in Vietnam. The nucleoprotein (N) and glycoprotein (G) genes of RVs were amplified from the brains of ten rabid dogs of Ho Chi Minh City, Vietnam. The nucleotide sequences of these genes were compared with those of other Asian stra...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2007.tb03979.x

    authors: Yamagata J,Ahmed K,Khawplod P,Mannen K,Xuyen DK,Loi HH,Dung NV,Nishizono A

    更新日期:2007-01-01 00:00:00

  • Influenza A virus protein PA-X suppresses host Ankrd17-mediated immune responses.

    abstract::Influenza A virus (IAV) PA-X is a critical ribonuclease protein involved in host cell shutoff but its role in modulating the host immune response to IAV infection remains to be addressed. In this study, host cellular proteins that directly interact with PA-X were screened to investigate the biological function of PA-X...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12863

    authors: Li M,Qi W,Chang Q,Chen R,Zhen D,Liao M,Wen J,Deng Y

    更新日期:2020-11-26 00:00:00

  • Effects of tungstosilicate on strains of methicillin-resistant Staphylococcus aureus with unique resistant mechanisms.

    abstract::In a previous study, it was found that polyoxotungstates such as undecatungstosilicate (SiW11) greatly sensitized strains of methicillin-resistant Staphylococcus aureus (MRSA) to beta-lactams. In this report, the effects of SiW11 on several MRSA strains with unique resistant mechanisms were studied. SiW11 was still ef...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2003.tb03388.x

    authors: Tajima Y

    更新日期:2003-01-01 00:00:00

  • Intracellular proliferation of clover yellow vein virus is unaffected by the recessive resistance gene cyv1 of Pisum sativum.

    abstract::The pea cyv1 gene is a yet-to-be-identified recessive resistance gene that inhibits the infection of clover yellow vein virus (ClYVV). Previous studies confirmed that the cell-to-cell movement of ClYVV is inhibited in cyv1-carrying pea plants; however, the effect of cyv1 on viral replication remains unknown. In this s...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12755

    authors: Taninaka Y,Nakahara KS,Hagiwara-Komoda Y

    更新日期:2020-01-01 00:00:00

  • Purification and characterization of Vibrio vulnificus protease.

    abstract::A protease was purified from a strain of Vibrio vulnificus isolated from the blood of a septicemic human. The vibrio was cultured in bacto peptone-yeast extract medium, and the protease was purified by a purification procedure including ultrafiltration of the culture supernatant with an Amicon YM 5 membrane, diethylam...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1987.tb03064.x

    authors: Miyoshi N,Shimizu C,Miyoshi S,Shinoda S

    更新日期:1987-01-01 00:00:00

  • Identification of influenza C virus phosphoproteins.

    abstract::The HMV-II cells infected with influenza C virus were labeled with inorganic [32P]phosphate to identify phosphorylated proteins. Analysis by radioimmunoprecipitation with antiviral serum or monoclonal antibodies revealed that three major structural proteins of the virus, hemagglutinin-esterase (HE), nucleoprotein (NP)...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1995.tb03251.x

    authors: Nishimura H,Sugawara K,Gao P,Muraki Y,Hongo S,Kitame F,Nakamura K

    更新日期:1995-01-01 00:00:00

  • In situ infiltration of natural killer-like cells induced by intradermal injection of the nucleic acid fraction from BCG.

    abstract::Intradermal injection of MY-1, a nucleic acid fraction extracted from Mycobacterium bovis strain BCG, induced in situ infiltration of mononuclear cells, most of which were asialo GM1 (GA1)-positive as determined by immunofluorescence microscopy. The infiltration occurred with as little as 1 microgram of MY-1 and laste...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1989.tb00980.x

    authors: Kuramoto E,Toizumi S,Shimada S,Tokunaga T

    更新日期:1989-01-01 00:00:00

  • Determination of minimum biofilm eradication concentrations of orbifloxacin for canine bacterial uropathogens over different treatment periods.

    abstract::Biofilm formation can cause refractory urinary tract infections (UTIs) in dogs; however, minimum biofilm eradication concentrations (MBECs) of veterinary drugs against canine uropathogens remain to be investigated. In this study, the MBECs of orbifloxacin (OBFX), trimethoprim-sulfamethoxazole (TMS) and amoxicillin/cla...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12461

    authors: Shimizu T,Harada K

    更新日期:2017-01-01 00:00:00

  • Virulence of representative Japanese Francisella tularensis and immunologic consequences of infection in mice.

    abstract::Francisella tularensis, which causes tularemia, is widely distributed in the Northern hemisphere. F. tularensis strains isolated in Japan are genetically unique from non-Japanese strains; however, their phenotypic properties have not been well studied. Thus, mice were infected with representative Japanese strains of F...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12363

    authors: Hotta A,Fujita O,Uda A,Yamamoto Y,Sharma N,Tanabayashi K,Yamada A,Morikawa S

    更新日期:2016-03-01 00:00:00

  • Spodiobacter cordis gen. nov. sp. nov., a member of the family Flavobacteriaceae isolated from patients with infective endocarditis.

    abstract::Two gram-negative, catalase-negative, oxidase-positive strains (PAGU 1467T and PAGU 1468) isolated from patients with infective endocarditis were investigated to determine their taxonomic status. 16S rRNA gene sequence analysis indicated that the two strains were members of the Bergeyella-Chryseobacterium-Riemerella b...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12673

    authors: Tomida J,Fujiwara N,Naka T,Morita Y,Sawabe E,Tojo N,Kikuchi K,Kawamura Y

    更新日期:2019-03-01 00:00:00

  • Analysis of the structural gene encoding a hemolysin in Vibrio mimicus.

    abstract::An environmental isolate of V. mimicus, strain E-33, has been reported to produce and secrete a hemolysin of 63 kDa. The hemolysin is enterotoxic in test animals. The nucleotide sequence of the structural gene of the hemolysin was determined. We found a 2,232 bp open reading frame, which codes a peptide of 744 amino a...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1997.tb01183.x

    authors: Rahman MM,Miyoshi S,Tomochika K,Wakae H,Shinoda S

    更新日期:1997-01-01 00:00:00

  • Epidemiology and Molecular Characterization of Chikungunya Virus from Human Cases in North India, 2016.

    abstract::Chikungunya virus (CHIKV) an arthropod-borne Alphavirus is responsible for chikungunya fever and joint pain etc. Arthralgia is a major symptom; some patients recover early while others suffer for a very long time. Epidemiology and molecular characterization of three whole-genome sequences of CHIKV have been carried ou...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12869

    authors: Khan N,Bhat R,Jain V,Raghuvendara B S,Patel AK,Ray P

    更新日期:2020-12-21 00:00:00

  • Process of consecutive cell divisions and separations in a regular tetrads-forming mutant of Micrococcus lysodeikticus (luteus).

    abstract::A mutant MT of Micrococcus lysodeikticus (luteus) IFO 3333, whose minimum growing unit is not a single cell, but a tetrad unlike the wild-type divides by binary fission of each monococcus, and then separates first into two daughter tetrads, second into four tetrads and third into eight tetrads. The three planes of eit...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1978.tb00391.x

    authors: Monodane T,Matsushima Y,Kotani S

    更新日期:1978-01-01 00:00:00

  • Characterization of an outer membrane protein gene, pgmA, and its gene product from Porphyromonas gingivalis.

    abstract::A gene upstream from fimA, the gene encoding fimbrilin, on the chromosome of Porphyromonas gingivalis was sequenced and shown to be the gene encoding an outer membrane protein in this organism based on homology and biochemical analyses. Therefore, the gene (formerly ORF5) was designated pgmA, the P. gingivalis outer m...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1999.tb03354.x

    authors: Hongo H,Osano E,Ozeki M,Onoe T,Watanabe K,Honda O,Tani H,Nakamura H,Yoshimura F

    更新日期:1999-01-01 00:00:00

  • Histopathological studies of reactions in mice injected with aluminum-adsorbed tetanus toxoid.

    abstract::Histopathological observations of changes at the injection site in mice were made for 20 weeks after a single intramuscular (im) injection of aluminum hydroxide-adsorbed tetanus toxoid (Alum-TT), plain tetanus toxoid (TT), or aluminum adjuvant (Alum alone). Marked injury to muscle fibers and infiltration of neutrophil...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1982.tb00261.x

    authors: Goto N,Akama K

    更新日期:1982-01-01 00:00:00

  • Roles of the interactions between Env and Gag proteins in the HIV-1 replication cycle.

    abstract::The Env and Gag proteins of HIV-1 are the two major structural proteins of this retrovirus. The interactions between Env and Gag proteins and their regulation in HIV-1 are required for several steps of the replication cycle, involving not only virus assembly, specifically Env incorporation, but also entry steps after ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1348-0421.2008.00008.x

    authors: Murakami T

    更新日期:2008-05-01 00:00:00

  • Regulation of marA, soxS, rob, acrAB and micF in Salmonella enterica serovar Typhimurium.

    abstract::Importance of the overexpression of AcrAB efflux pumps in the low-level resistance of pathogens to antimicrobials requires a better understanding of the AcrAB regulation. The goal of the present research was to study the transcription of acrAB, as well as the genes that play a role in its regulation in Salmonella ente...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2008.00075.x

    authors: Hartog E,Ben-Shalom L,Shachar D,Matthews KR,Yaron S

    更新日期:2008-12-01 00:00:00