Abstract:
:The pea cyv1 gene is a yet-to-be-identified recessive resistance gene that inhibits the infection of clover yellow vein virus (ClYVV). Previous studies confirmed that the cell-to-cell movement of ClYVV is inhibited in cyv1-carrying pea plants; however, the effect of cyv1 on viral replication remains unknown. In this study, we developed a new pea protoplast transfection method to investigate ClYVV propagation at the single-cell level. Using this method, we revealed that ClYVV accumulates to similar levels in both ClYVV-susceptible and cyv1-carrying pea protoplasts. Thus, the cyv1-mediated resistance would not suppress intracellular ClYVV replication.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Taninaka Y,Nakahara KS,Hagiwara-Komoda Ydoi
10.1111/1348-0421.12755subject
Has Abstractpub_date
2020-01-01 00:00:00pages
76-82issue
1eissn
0385-5600issn
1348-0421journal_volume
64pub_type
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