Analysis of human T-cell antigens by one- and two-dimensional polyacrylamide gel electrophoresis.

abstract：:The combination of a Legionella pneumophila culture isolation technique and macrophage infectivity potentiator (mip) gene-specific nested polymerase chain reaction (PCR) is pivotal for effective routine use in an environmental water system laboratory. Detection of Legionella organisms in 169 environmental samples was ...

journal_title：Microbiology and immunology

authors： Nintasen R,Utrarachkij F,Siripanichgon K,Bhumiratana A,Suzuki Y,Suthienkul O

更新日期：2007-01-01 00:00:00

abstract：:The antigenic formula and chemical structure of capsular polysaccharide (CPS) of Cryptococcus albidus var. albidus (C. albidus) were studied in relation to those of C. neoformans var. neoformans serotype A (C. neoformans A). The results of slide agglutination tests with factor sera and reciprocal adsorption experiment...

journal_title：Microbiology and immunology

authors： Ikeda R,Matsuyama H,Nishikawa A,Shinoda T,Fukazawa Y

更新日期：1991-01-01 00:00:00

abstract：:We analyzed the mechanism of spontaneous B cell activation in lupus mice by using anticlass-II antibody in vitro. The in vitro culture of B cells from old NZB mice markedly produced Ig without any stimulation, while B cells from NZW mice did not. The addition of anticlass-II antibody (anti-Iad antibody) to the culture...

journal_title：Microbiology and immunology

authors： Saito K,Tanaka Y,Ota T,Eto S,Yamashita U

更新日期：1991-01-01 00:00:00

abstract：:Macrophages are extremely heterogeneous mononuclear phagocytes widely distributed throughout the body. They play unique roles in each organ where they reside. Among macrophage subsets, red pulp macrophages (RPMs) that localize in the splenic red pulp, are critical for maintenance of blood homeostasis by actively phago...

journal_title：Microbiology and immunology

authors： Kurotaki D,Uede T,Tamura T

更新日期：2015-02-01 00:00:00

abstract：:The mechanism of MHC-restricted T and B cell interactions in antibody response was studied with IgM-inducible B hybridomas and antigen-specific helper T cell clones. B hybridomas were prepared by fusion between splenic B cells from (CBA/N (H-2k) X BALB/c (H-2d)) F1 (NBF1) male mice and a B lymphoma cell line, M12.4.5....

journal_title：Microbiology and immunology

authors： Yoshikubo T,Kimoto M,Kishimoto T

更新日期：1985-01-01 00:00:00

abstract：:Cholesterol is necessary for the conversion of Vibrio cholerae hemolysin (VCH) monomers into oligomers in liposome membranes. Using different sterols, we determined the stereochemical structures of the VCH-binding active groups present in cholesterol. The VCH monomers are bound to cholesterol, diosgenin, campesterol, ...

journal_title：Microbiology and immunology

authors： Ikigai H,Otsuru H,Yamamoto K,Shimamura T

更新日期：2006-01-01 00:00:00

abstract：:The distribution of the antibody to influenza C virus in the dogs and pigs in Yamagata prefecture, Japan was investigated by using three different serological methods: hemagglutination-inhibition (HI), radioimmunoprecipitation (RIP), and immunoblotting. The antibody against influenza C virus glycoprotein (gp88) was de...

journal_title：Microbiology and immunology

authors： Ohwada K,Kitame F,Sugawara K,Nishimura H,Homma M,Nakamura K

更新日期：1987-01-01 00:00:00

abstract：:The HMV-II cells infected with influenza C virus were labeled with inorganic [32P]phosphate to identify phosphorylated proteins. Analysis by radioimmunoprecipitation with antiviral serum or monoclonal antibodies revealed that three major structural proteins of the virus, hemagglutinin-esterase (HE), nucleoprotein (NP)...

journal_title：Microbiology and immunology

authors： Nishimura H,Sugawara K,Gao P,Muraki Y,Hongo S,Kitame F,Nakamura K

更新日期：1995-01-01 00:00:00

abstract：:Noroviruses cause acute gastroenteritis. Since multiple genotypes of norovirus co-circulate in humans, changing the genotype composition and eluding host immunity, development of a polyvalent vaccine against norovirus in which the genotypes of vaccine strains match the major strains in circulation in the target season...

journal_title：Microbiology and immunology

authors： Suzuki Y,Doan YH,Kimura H,Shinomiya H,Shirabe K,Katayama K

更新日期：2016-06-01 00:00:00

abstract：:The genus Enhydrobacter, first reported as a member of the family Vibrionaceae, has been placed in the family Moraxellaceae, but as a genus incertae sedis in Bergey's Manual of Systematic Bacteriology 2nd edition. During our taxonomic investigation of Enhydrobacter-like organisms, we observed that the 16S rRNA sequenc...

journal_title：Microbiology and immunology

authors： Kawamura Y,Fujiwara N,Naka T,Mitani A,Kubota H,Tomida J,Morita Y,Hitomi J

更新日期：2012-01-01 00:00:00

abstract：:Previous studies have demonstrated that the murine monoclonal antibody (MoAb) NM-01 activates the human complement classical pathway resulting in lysis of human immunodeficiency virus (HIV). The present study was performed to determine the availability of the V3-loop of gp120 relative to the complement regulatory prot...

journal_title：Microbiology and immunology

authors： Nakamura M,Okada H,Sasaki H,Yoshida K,Kamada M,Okada N,Terada M,Ohno T

更新日期：1996-01-01 00:00:00

abstract：:Zymographic analysis was performed to know the bacteriolytic enzyme profiles of 4% SDS extracts of oral streptococci, Streptococcus mutans, S. sobrinus, S. sanguis, S. mitis and S. salivarius. We investigated the five strains in each species and found that the profile was very similar among strains of the same species...

journal_title：Microbiology and immunology

authors： Yoshimura G,Komatsuzawa H,Kajimura J,Fujiwara T,Ohara M,Kozai K,Sugai M

更新日期：2004-01-01 00:00:00

abstract：:Burkholderia cepacia complex (Bcc) are opportunistic pathogens implicated with nosocomial infections, and high rates of morbidity and mortality, especially in individuals with cystic fibrosis (CF). B. cepacia are naturally resistant to different classes of antibiotics, and can subvert the host innate immune responses ...

journal_title：Microbiology and immunology

authors： Ganesh PS,Vishnupriya S,Vadivelu J,Mariappan V,Vellasamy KM,Shankar EM

更新日期：2020-02-01 00:00:00

abstract：:BALB/c mice were immunized intraperitoneally with the food antigen ovalbumin (OVA) while they were fed with Lactobacillus GG heated killed cell preparation. The oral administration of Lactobacillus GG did not appear to modify the antigen-augmented serum IgE in the tested mice but significantly augmented serum OVA spec...

journal_title：Microbiology and immunology

authors： He F,Morita H,Kubota A,Ouwehand AC,Hosoda M,Hiramatsu M,Kurisaki J

更新日期：2005-01-01 00:00:00

abstract：:The effects of added maltose on the activities of a preparation of crude glucosyltransferases (GTases) and purified dextransucrase (DS) were investigated to elucidate the inhibition mechanism of maltose on the synthesis of water-insoluble glucan (ISG) in Streptococcus mutans HS-6. Tri- and tetra-saccharides produced b...

journal_title：Microbiology and immunology

authors： Fukui K,Moriyama T

更新日期：1983-01-01 00:00:00

abstract：:Japanese encephalitis virus (JEV) core protein is detected not only in the cytoplasm but also in the nucleoli of infected cells. We previously showed that a mutant JEV lacking the nucleolar localization of the core protein impaired viral replication in mammalian cells. In this study, we identified a nucleolar phosphop...

journal_title：Microbiology and immunology

authors： Tsuda Y,Mori Y,Abe T,Yamashita T,Okamoto T,Ichimura T,Moriishi K,Matsuura Y

更新日期：2006-01-01 00:00:00

abstract：:The effect of host cell factors on infectivity of human immunodeficiency virus type 1 (HIV-1) was studied by infecting a monoblastoid cell line (U937) or a T-cell line (MOLT-4) with a highly infective single clone of HIV-1 and comparing the infectivity of the produced viruses to different cell lines. Chronically infec...

journal_title：Microbiology and immunology

authors： el-Farrash MA,Masuda T,Kuroda MJ,Harada S

更新日期：1993-01-01 00:00:00

abstract：:The botulinum neurotoxin light chain (BoNT-LC) is a zinc-dependent metalloprotease that cleaves neuronal SNARE proteins such as SNAP-25, VAMP2, and Syntaxin1. This cleavage interferes with the neurotransmitter release of peripheral neurons and results in flaccid paralysis. SNAP, VAMP, and Syntaxin are representative o...

journal_title：Microbiology and immunology

authors： Yamamoto H,Ida T,Tsutsuki H,Mori M,Matsumoto T,Kohda T,Mukamoto M,Goshima N,Kozaki S,Ihara H

更新日期：2012-04-01 00:00:00

abstract：:In Streptococcus pneumoniae, heat shock induces the synthesis of 65-, 73-, and 84-kDa proteins, and ethanol shock induces a 104-kDa protein. In this study, the 65-, 84-, and 104-kDa proteins were identified as members of the GroEL, ClpL and alcohol dehydrogenase families, respectively, and the general properties of th...

journal_title：Microbiology and immunology

authors： Choi IH,Shim JH,Kim SW,Kim SN,Pyo SN,Rhee DK

更新日期：1999-01-01 00:00:00

abstract：:Recombinant human interferon-gammas (rHuIFN-gamma s) were obtained from two different mammalian cells (mouse C127 cells and Chinese hamster ovary, CHO, cells) cultured in a microcarrier culture system. Both rHuIFN-gamma s were purified using sequential chromatographies for their comparison of structural properties. Th...

journal_title：Microbiology and immunology

authors： Sano E,Kamata K,Okano K,Yamazaki S,Iizuka M,Kobayashi S

更新日期：1988-01-01 00:00:00

abstract：:Soil specimens obtained from several areas of Japan, which are closely located to or facing the Continental land of China, were examined for the distribution of Clostridium botulinum, especially pertaining to types A and B. A total of 266 specimens of Japan, when cultured, showed no type A or B toxicity, although 30 (...

journal_title：Microbiology and immunology

authors： Yamakawa K,Kamiya S,Nishida S,Yoshimura K,Yu H,Lu DY,Nakamura S

更新日期：1988-01-01 00:00:00

abstract：:Lactobacillus casei strain Shirota (LcS) is a widely used probiotic strain with health benefits. In this study, the survival of LcS in the intestines of healthy Chinese adults was assessed and the effects of LcS on stool consistency, stool SCFAs and intestinal microbiota evaluated. Subjects consumed 100 mL per day of ...

journal_title：Microbiology and immunology

authors： Wang R,Chen S,Jin J,Ren F,Li Y,Qiao Z,Wang Y,Zhao L

更新日期：2015-05-01 00:00:00

abstract：:The present study was conducted to determine the possible role of intracellular Ca2+ in lipid peroxide formation in endotoxin-poisoned mice. Leakages of LDH isozyme and acid phosphatase in serum of mice fed a Ca2+-deficient diet were remarkably increased after administration of 200 micrograms of endotoxin compared to ...

journal_title：Microbiology and immunology

authors： Sakaguchi S,Ibata H,Yokota K

更新日期：1989-01-01 00:00:00

abstract：:Autoantibodies to IgE and beta2-adrenergic receptor have been reported in patients with allergic rhinitis. To investigate whether autoimmunity in allergic rhinitis is directed to such limited molecules or directed to a wide range of self proteins, we here attempted to survey autoantigens/autoantibodies comprehensively...

journal_title：Microbiology and immunology

authors： Nakamura M,Tsutsumi K,Ooka S,Sekine T,Koizuka I,Nishioka K,Kato T

更新日期：2004-01-01 00:00:00

abstract：:The aim of this study was to investigate the pathogenesis of Mycoplasma pneumoniae (MP) infection and its association with cardiac and hepatic damage. Between March 2013 and March 2014, 59 children with MP pneumonia (MPP) and 30 healthy children were enrolled. Serum titers of TLR4, T cell immunoglobulin and mucin-doma...

journal_title：Microbiology and immunology

authors： Fan Q,Meng J,Li P,Liu Z,Sun Y,Yan P

更新日期：2015-07-01 00:00:00

abstract：:Using a mouse monoclonal antibody (MAb) 2F raised against Vibrio cholerae non-O1 heat-stable enterotoxin (NAG-ST) which also recognizes a shared epitope of Yersinia enterocolitica heat-stable enterotoxin (Y-ST), a competitive enzyme-linked immunosorbent assay (ELISA) was developed for independent detection of NAG-ST a...

journal_title：Microbiology and immunology

authors： Nair GB,Bhattacharya SK,Takeda T

更新日期：1993-01-01 00:00:00

abstract：:We cloned and sequenced two new Verotoxin 2 (VT2) variant genes: one from an Escherichia coli strain from a case of bovine diarrhea and the other from an E. coli strain from a patient with diarrhea. The nucleotide and amino acid sequences of these two genes were highly homologous with, but distinct from those of the V...

journal_title：Microbiology and immunology

authors： Lin Z,Yamasaki S,Kurazono H,Ohmura M,Karasawa T,Inoue T,Sakamoto S,Suganami T,Takeoka T,Taniguchi Y

更新日期：1993-01-01 00:00:00

abstract：:M protein is an important virulence determinant in Streptococcus pyogenes, but the amounts of M protein in various strains of the species remain to be elucidated. To assess the amount of M protein in strains of each emm genotype, dot blot analysis was performed on 141 clinically isolated strains. Among the cell membra...

journal_title：Microbiology and immunology

authors： Matsumoto M,Suzuki M,Hirose K,Hiramatsu R,Minagawa H,Minami M,Tatsuno I,Okamoto A,Ohta M,Hasegawa T

更新日期：2011-06-01 00:00:00

abstract：:The amino acid sequence of the principal neutralizing determinant (PND) of 224 cases of human immunodeficiency virus type 1 (HIV-1) was determined and the most frequently occurring sequence was used as a peptide antigen for studying virus-specific antibody responses. In our present study, a linear peptide of the most ...

journal_title：Microbiology and immunology

authors： Bukawa H,Fukushima J,Hamajima K,Kimura M,Tsuji T,Xin KQ,Okuda K

更新日期：1995-01-01 00:00:00

abstract：:Exocellular proteases produced by Vibrio fluvialis, V. furnissii, V. metschnikovii and V. campbellii were characterized and compared to those of V. mimicus protease (VMP) and V. vulnificus protease (VVP). These proteases possessed both elastolytic and hemagglutinating abilities and were identified, except that of V. m...

journal_title：Microbiology and immunology

authors： Alam M,Miyoshi S,Shinoda S

更新日期：1995-01-01 00:00:00