Cloning and sequencing of two new Verotoxin 2 variant genes of Escherichia coli isolated from cases of human and bovine diarrhea.

abstract：:A solubilized sheep red blood cell (SRBC) antigen (supernatant fraction obtained by centrifuging 10(7)-2 x 108 sonicated SRBC at 6 x 10(4) g for 30 min [Sup-SRBC]), whose ability to inhibit anti-SRBC plaque formation was 70% of that of the original sonicated SRBC, was unable to elicit a detectable antibody response in...

journal_title：Microbiology and immunology

authors： Nagase F,Nakashima I,Nagase N,Kato N

更新日期：1982-01-01 00:00:00

abstract：:We examined the mechanisms of fluconazole resistance in a fluconazole-resistant Candida albicans isolate from a Japanese patient with chronic mucocutaneous candidiasis. It was demonstrated that the highly resistant phenotype of this strain was associated with combined mechanisms of the energy-dependent reduced intrace...

journal_title：Microbiology and immunology

authors： Kamai Y,Maebashi K,Kudoh M,Makimura K,Naka W,Uchida K,Yamaguchi H

更新日期：2004-01-01 00:00:00

abstract：:Japanese Lyme borrelias classified as ribotype IV is predominant among isolates derived from clinical specimens, reservoir rodents and Ixodes persulcatus ticks, and has been characterized as Borrelia garinii. These B. garinii isolates have antigenic and genetic features apparently different from North American, Europe...

journal_title：Microbiology and immunology

authors： Wang J,Masuzawa T,Komikado T,Yanagihara Y

更新日期：1997-01-01 00:00:00

abstract：:There are two types of carboxypeptidases present in human blood, carboxypeptidase N (CPN) and arginine carboxypeptidase (CPR). CPR is generated during coagulation from a precursor (proCPR) which can be converted to the active form by trypsin in vitro. Since it is difficult to distinguish the two types of carboxypeptid...

journal_title：Microbiology and immunology

authors： Guo X,Morioka A,Kaneko Y,Okada N,Obata K,Nomura T,Campbell W,Okada H

更新日期：1999-01-01 00:00:00

abstract：:The Clostridium perfringens strain 13 genome contains two genes (fbpA, fbpB) that encode putative Fbp. Both rFbpA and rFbpB were purified and their reactivity with human serum Fn was analyzed. To determine the region of the Fn molecule recognized by rFbp, a plate binding assay using N-terminal 70-kDa peptide, III1-C p...

journal_title：Microbiology and immunology

authors： Yamasaki T,Hitsumoto Y,Katayama S,Nogami Y

更新日期：2010-04-01 00:00:00

abstract：:Accurate and rapid detection of carbapenemases and identification of their types in Enterobacteriaceae are both still major challenges for clinical laboratories in attempting to prevent the intrusion and transmission of carbapenemase-producing Enterobacteriaceae. This study aimed to evaluate the performance of the MAS...

journal_title：Microbiology and immunology

authors： Ohsaki Y,Kubo R,Hobson J,Davies M,Osaka S,Hayashi W,Taniguchi Y,Koide S,Nagano Y,Nagano N

更新日期：2018-01-01 00:00:00

abstract：:To investigate cellular components incorporated into the rabies virion, monoclonal antibodies (MAbs) were screened based on their reactivity with additional virion components. Two of the MAbs we prepared recognized a virion-associated 21 kDa polypeptide (referred to as VAP21) from a BHK-21 cell. Since the MAbs precipi...

journal_title：Microbiology and immunology

authors： Sagara J,Tochikura TS,Yamamoto T,Tsukita S,Tsukita S,Kawai A

更新日期：1997-01-01 00:00:00

abstract：:Many studies have proved the relevance of local immune responses, rather than systemic immunity, to the pathogenesis of allergic rhinitis. Indeed, allergen-specific B lymphocyte undergoes class switching to IgE in situ. However, the relative contribution of in situ production to the amount in serum is still ambiguous....

journal_title：Microbiology and immunology

authors： Yoshida T,Usui A,Kusumi T,Inafuku S,Sugiyama T,Koide N,Yokochi T

更新日期：2005-01-01 00:00:00

abstract：:Erythematous delayed reactions without induration, presumably assigned to Jones-Mote type, were characterized by the resistance to treatment with cyclophosphamide (CY) before elicitation or immunization in guinea pigs immunized with BGG in IFA or CFA. CY-treatment before elicitation converted delayed erythematous reac...

journal_title：Microbiology and immunology

authors： Himeno K,Nomoto K,Takeya K

更新日期：1978-01-01 00:00:00

abstract：:The biological effect of purified beta toxin of Clostridium perfringens type C in vivo was investigated. After intravenous injection of the purified beta toxin into rats, a rise in blood pressure and a simultaneous fall in heart rate were observed. After the blood pressure reached a maximum, the heart rate recovered g...

journal_title：Microbiology and immunology

authors： Sakurai J,Fujii Y,Matsuura M,Endo K

更新日期：1981-01-01 00:00:00

abstract：:A VeraCode-allele-specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)-DNA. Oligonucleotide primers containing HPV-type-specific L1 sequences were annealed to HPV-DNA amplified by PGMY-PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The...

journal_title：Microbiology and immunology

authors： Kitamura-Muramatsu Y,Kusumoto-Matsuo R,Kondo K,Mori S,Saito S,Tsukahara Y,Kukimoto I

更新日期：2012-02-01 00:00:00

abstract：:Susceptibility of eight strains of influenza A and B viruses to interferon and to poly(I) . poly(C) were determined by the plaque reduction method. All strains tested were slightly less susceptible than vesicular stomatitis virus (VSV) in an established line of canine kidney (MDCK) cells. The 50% plaque depression dos...

journal_title：Microbiology and immunology

authors： Machida H,Kuninaka A,Yoshino H

更新日期：1980-01-01 00:00:00

abstract：:Autoantibodies to myeloperoxidase (MPO) are a subset of anti-neutrophil cytoplasmic antibody (ANCA, MPO-ANCA) detected in the sera of some patients with primary systemic vasculitis. The titer of MPO-ANCA does not always reflect disease activity and this inconsistency may be attributable to differences in epitopic spec...

journal_title：Microbiology and immunology

authors： Suzuki K,Kobayashi S,Yamazaki K,Gondo M,Tomizawa K,Arimura Y,Nakabayashi K,Ozaki S,Yoshida M,Yoshida T,Tsusaka N,Muso E,Okazaki T,Hashimoto H

更新日期：2007-01-01 00:00:00

abstract：:We have previously identified a heterodimer molecule, Z-1, on guinea pig peritoneal macrophages (M phi s) by the newly prepared monoclonal antibody, anti-Z-1, and Z-1 has been assumed to be the complement receptor type three (CR3) in this species. To clarify this assumption, the cell type distribution of the antigen i...

journal_title：Microbiology and immunology

authors： Tamoto K,Nochi H,Tada M,Kakinuma T,Mori Y

更新日期：1993-01-01 00:00:00

abstract：:The effects of B subunit of cholera toxin (CTB) on delayed-type hypersensitivity (DTH) response to influenza vaccine derived from influenza virus A/PR/8/34 (PR-8, HlNl) virus were investigated in B10 mice that were immunized intranasally with both influenza vaccine and CTB. The result showed that intranasal inoculatio...

journal_title：Microbiology and immunology

authors： Kikuta K,Kurata H,Nagamine T,Aizawa C,Ueno Y,Kurata T,Tamura S

更新日期：1990-01-01 00:00:00

abstract：:BALB/c mice were immunized intraperitoneally with the food antigen ovalbumin (OVA) while they were fed with Lactobacillus GG heated killed cell preparation. The oral administration of Lactobacillus GG did not appear to modify the antigen-augmented serum IgE in the tested mice but significantly augmented serum OVA spec...

journal_title：Microbiology and immunology

authors： He F,Morita H,Kubota A,Ouwehand AC,Hosoda M,Hiramatsu M,Kurisaki J

更新日期：2005-01-01 00:00:00

abstract：:The IFN-induced double-stranded RNA (dsRNA)-activated protein kinase PKR is one of the key molecules in the antiviral effects of IFN. To clarify the effects of hepatitis C virus nonstructural protein 5A (NS5A) on antiviral activity of IFN, in particular on PKR kinase activity, in mammalian cells, we established induci...

journal_title：Microbiology and immunology

authors： Noguchi T,Satoh S,Noshi T,Hatada E,Fukuda R,Kawai A,Ikeda S,Hijikata M,Shimotohno K

更新日期：2001-01-01 00:00:00

abstract：:Site-specific recombinase is widely applied for the regulation of gene expression because its regulatory action is strict and efficient. However, each system can mediate regulation of only one gene at a time. Here, we demonstrate efficient "sequential" gene regulation using Cre-and FLP-expressing recombinant adenoviru...

journal_title：Microbiology and immunology

authors： Kondo S,Takahashi Y,Shiozawa S,Ichise H,Yoshida N,Kanegae Y,Saito I

更新日期：2006-01-01 00:00:00

abstract：:Although most influenza vaccines are produced in eggs, new types of vaccines must be developed. In this study, the immunogenicity and safety of a baculovirus-expressed hemagglutinin (HA) of H1N1 influenza virus (Korea/01/2009; designated "HA-Bac-K") was compared with those of a commercially available baculovirus-expre...

journal_title：Microbiology and immunology

authors： Na HN,Kim KH,Song MK,Park HL,Lee EY,Shim SH,Park S,Nam JH

更新日期：2013-09-01 00:00:00

abstract：:An environmental isolate of V. mimicus, strain E-33, has been reported to produce and secrete a hemolysin of 63 kDa. The hemolysin is enterotoxic in test animals. The nucleotide sequence of the structural gene of the hemolysin was determined. We found a 2,232 bp open reading frame, which codes a peptide of 744 amino a...

journal_title：Microbiology and immunology

authors： Rahman MM,Miyoshi S,Tomochika K,Wakae H,Shinoda S

更新日期：1997-01-01 00:00:00

abstract：:During the three-year period from 1984 to 1987, 506 acute gastroenteritis outbreaks involving 14,383 patients were reported to the Bureau of Public Health, Tokyo Metropolitan Government. Eighty (4,324 patients) of 150 outbreaks (4,860 patients) from which etiologic agents were not identified were subjected to virologi...

journal_title：Microbiology and immunology

authors： Sekine S,Okada S,Hayashi Y,Ando T,Terayama T,Yabuuchi K,Miki T,Ohashi M

更新日期：1989-01-01 00:00:00

abstract：:Several liposomes containing ovalbumin (OVA), a model antigen, with different lipid compositions were prepared in order to evaluate their ability to induce oral tolerance. Oral administration of these liposomal OVAs induced suppression of the proliferative responses of popliteal lymph node cells from the treated mice ...

journal_title：Microbiology and immunology

authors： Masuda K,Horie K,Suzuki R,Yoshikawa T,Hirano K

更新日期：2002-01-01 00:00:00

abstract：:Specific tumor microenvironment signaling might prevent the maturation of dendritic cells (DCs) with tolerogenic and immunosuppressive potential accounting for antigen-specific unresponsiveness in the lymphoid organs and in the periphery. In the present study, dendritic cells treated with LLC lung cancer cell or 4T1 b...

journal_title：Microbiology and immunology

authors： Ma C,Su M,Shen K,Chen J,Ning Y,Qi C

更新日期：2020-01-01 00:00:00

abstract：:We previously reported a helper T-cell (Th) epitope (peptide i) which corresponded to the sequence ranging from positions 462 to 479 from the N-terminus of the Friend-murine leukemia virus (F-MuLV) envelope protein (env462-479). Homologous sequences exist in both Moloney-murine leukemia (M-MuLV env452-469) and endogen...

journal_title：Microbiology and immunology

authors： Uenishi H,Iwanami N,Yamagishi H,Nakatani T,Kawasaki T,Tamamura H,Fujii N,Kuribayashi K

更新日期：1998-01-01 00:00:00

abstract：:Farnesol is well known as a quorum-sensing molecule of Candida albicans. To assess the pathological function of farnesol, its effects on macrophage viability and functions including growth inhibitory activities against C. albicans were examined in vitro. Murine macrophages, when cultured in the presence of 56-112 micr...

journal_title：Microbiology and immunology

authors： Abe S,Tsunashima R,Iijima R,Yamada T,Maruyama N,Hisajima T,Abe Y,Oshima H,Yamazaki M

更新日期：2009-06-01 00:00:00

abstract：:We studied the heat resistance and the range of growth temperature o gram-negative rods to find one of the bacterial factors governing their infectivity in exogenous and endogenous infections in predisposed patients. Escherichia coli, Klebsiella pneumoniae, serratia marcescens, Pseudomonas aeruginosa, and Acinetobacte...

journal_title：Microbiology and immunology

authors： Tsuji A,Kaneko Y,Takahashi K,Ogawa M,Goto S

更新日期：1982-01-01 00:00:00

abstract：:Sera from 3,158 individuals living in northern Egypt were tested for the presence of antibodies against human T-lymphotropic virus type I (HTLV-I) by the newly developed particle agglutination (PA) test. Ten sera gave a positive reaction in the PA test. Eight of these sera were examined further by Western blotting and...

journal_title：Microbiology and immunology

authors： el-Farrash MA,Badr MF,Hawas SA,el-Nashar NM,Imai J,Komoda H,Hinuma Y

更新日期：1988-01-01 00:00:00

abstract：:The test for susceptibility to kanamycin is useful for differentiating between the genera Rhodococcus and Nocardia. All rhodococci except R. equi, R. erythropolis, and R. aurantiacus are susceptible to kanamycin, whereas all nocardiae except N. otitidis-caviarum are resistant to kanamycin. Tests for susceptibility to ...

journal_title：Microbiology and immunology

authors： Tsukamura M

更新日期：1988-01-01 00:00:00

abstract：:Natural killer cells, a critical component of the innate immune system, eradicate both virus-infected cells and tumor cells through cytotoxicity and secretion of cytokines. Human NK cell research has largely been based on in vitro studies because of the lack of appropriate animal models. In this study, a selective pro...

journal_title：Microbiology and immunology

authors： Hattori S,Matsuda K,Kariya R,Harada H,Okada S

更新日期：2016-02-01 00:00:00

abstract：:A mouse model for the study of postexposure prophylaxis of rabies was established. Mice injected intramuscularly with a street strain of rabies virus were significantly protected from death by five daily 0.2-ml doses of inactivated rabies vaccine of chick embryo cell culture origin initiated immediately or 3 hr after ...

journal_title：Microbiology and immunology

authors： Mifune K,Shichijo A,Makino Y,Takeuchi E,Yamada A,Sakamoto K

更新日期：1980-01-01 00:00:00