Novel multiplexed genotyping of human papillomavirus using a VeraCode-allele-specific primer extension method.

abstract：:Seroprevalence of Bartonella henselae was investigated in Japan in 48 individuals clinically suspected of having cat-scratch disease (CSD), 159 patients with cardiovascular diseases, and 129 healthy veterinary students. Of 48 CSD-suspected patients examined, 19 (39.6%) were positive for B. henselae-IgG and 4 (8.3%) fo...

journal_title：Microbiology and immunology

authors： Kikuchi E,Maruyama S,Sakai T,Tanaka S,Yamaguchi F,Hagiwara T,Katsube Y,Mikami T

更新日期：2002-01-01 00:00:00

abstract：:Absorption of a pooled human gamma globulin preparation with acetone-treated measles virus-infected cells removed all antibodies to measles virus antigens except a portion of the antibody to the fusion (F) protein. The residual anti-F antibody had hemolysis-inhibiting and virus-neutralizing activities, inhibited sprea...

journal_title：Microbiology and immunology

authors： Sato TA,Kohama T,Sugiura A

更新日期：1989-01-01 00:00:00

abstract：:Salmonella typhimurium strain 9 produces mini-cells during cell proliferation. Mini-cells are viable but cannot proliferate since they do not contain chromosomal DNA. Effects of whole cells and mini-cells of S. typhimurium on the immune responses were investigated, with the following results. Phagocytosis of peritonea...

journal_title：Microbiology and immunology

authors： Kurashige S,Yoshida T,Kodama K,Mitsuhashi S

更新日期：1981-01-01 00:00:00

abstract：:The genospecies Borrelia afzelii was isolated from a patient of Lyme disease in Hokkaido, Japan, for the first time, by culturing the minced erythema lesion in BSK II medium. Two analytical methods, rRNA gene restriction fragment length polymorphism (RFLP) and polymerase chain reaction (PCR) using the specific primer ...

journal_title：Microbiology and immunology

authors： Nakao M,Miyamoto K,Fukunaga M,Hashimoto Y,Takahashi H

更新日期：1994-01-01 00:00:00

abstract：:The effect of fatty acids on Mycobacterium smegmatis was examined in vitro at pH 5.0 to 7.0 to determine the role of fatty acids in the intracellular killing of mycobacteria. Unsaturated fatty acids showed strong bactericidal activity in low concentrations (0.005 to 0.02 mM), whereas saturated fatty acids, except for ...

journal_title：Microbiology and immunology

authors： Kanetsuna F

更新日期：1985-01-01 00:00:00

abstract：:Corilagin and tellimagrandin I are polyphenols isolated from the extract of Arctostaphylos uvaursi and Rosa canina L. (rose red), respectively. We have reported that corilagin and tellimagrandin I remarkably reduced the minimum inhibitory concentration (MIC) of beta-lactams in methicillin-resistant Staphylococcus aure...

journal_title：Microbiology and immunology

authors： Shiota S,Shimizu M,Sugiyama J,Morita Y,Mizushima T,Tsuchiya T

更新日期：2004-01-01 00:00:00

abstract：:Vibrio vulnificus infection has attracted special interest because of its high mortality. A strong clinical association exists between hepatic dysfunction and increased morbidity and mortality from V. vulnificus infection. In this study, the effect of C-reactive protein (CRP), a typical hepatogenic acute phase protein...

journal_title：Microbiology and immunology

authors： Chae MR,Park BH,Kim JS,Rho HW,Park JW,Kim HR

更新日期：2000-01-01 00:00:00

abstract：:Us3 is a serine-threonine protein kinase that is encoded by herpes simplex virus 1 (HSV-1). In experimental animal models of HSV infection, peripheral and intracranial inoculations can be used to study viral pathogenicity in peripheral sites (e.g., eyes and vagina) and central nervous systems (CNSs), respectively. In ...

journal_title：Microbiology and immunology

authors： Koyanagi N,Imai T,Arii J,Kato A,Kawaguchi Y

更新日期：2014-01-01 00:00:00

abstract：:The use of continuous free-flow electrophoresis for the purification of extracted lipopolysaccharides ( LPSs ) was investigated. Commercial (nucleic acid contaminated) LPS preparations, isolated by the hot phenol-water method of Westphal from Salmonella typhimurium and Escherichia coli 0111: B4, were analyzed. Continu...

journal_title：Microbiology and immunology

authors： Kuwae T,Kurata M

更新日期：1984-01-01 00:00:00

abstract：:A gene for protein Arp4, an IgA receptor protein derived from Streptococcus pyogenes AP4, was expressed in Escherichia coli. The product was demonstrated to be accumulated in a periplasmic space as a polypeptide with an apparent molecular weight of 40 kDa with the deleted C-terminal membrane anchor portion of protein ...

journal_title：Microbiology and immunology

authors： Nakatani T,Higuchi A,Noguchi H

更新日期：1992-01-01 00:00:00

abstract：:We previously reported the establishment of an HEp2 cell line which expresses the US3 protein kinase (PK) of herpes simplex virus type 2 (HSV-2) upon induction with IPTG. Here we report that expression, phosphorylation and ubiquitination of cytokeratin 17 (CK17) are enhanced in US3-expressing HEp2 cells. In vitro kina...

journal_title：Microbiology and immunology

authors： Murata T,Goshima F,Nishizawa Y,Daikoku T,Takakuwa H,Ohtsuka K,Yoshikawa T,Nishiyama Y

更新日期：2002-01-01 00:00:00

abstract：:Many studies have proved the relevance of local immune responses, rather than systemic immunity, to the pathogenesis of allergic rhinitis. Indeed, allergen-specific B lymphocyte undergoes class switching to IgE in situ. However, the relative contribution of in situ production to the amount in serum is still ambiguous....

journal_title：Microbiology and immunology

authors： Yoshida T,Usui A,Kusumi T,Inafuku S,Sugiyama T,Koide N,Yokochi T

更新日期：2005-01-01 00:00:00

abstract：:Coagulase negative staphylococci (CNS) are significant pathogens, particularly in medical device related infections and in immunocompromised patients. Five CNS strains were isolated from 5 NOS2 knockout mice with dermatitis. Histologically, granulomatous dermatitis was found in the skin around the ear with epidermal u...

journal_title：Microbiology and immunology

authors： Won YS,Kwon HJ,Oh GT,Kim BH,Lee CH,Park YH,Hyun BH,Choi YK

更新日期：2002-01-01 00:00:00

abstract：:Irradiation treatment enhanced resistance of C57BL/6, but not BALB/c against Toxoplasma gondii infection. Six Gy-irradiated (IR) C57BL/6 recipients of B-2 cells from T. gondii-infected C57BL/6 died after infection. B-2 suppressor cells from infected C57BL/6 enhanced production of IL-4 and IL-10 in peritoneal exudate c...

journal_title：Microbiology and immunology

authors： Mun HS,Aosai F,Fang H,Piao LX,Winn T,Norose K,Yano A

更新日期：2005-01-01 00:00:00

abstract：:Specific tumor microenvironment signaling might prevent the maturation of dendritic cells (DCs) with tolerogenic and immunosuppressive potential accounting for antigen-specific unresponsiveness in the lymphoid organs and in the periphery. In the present study, dendritic cells treated with LLC lung cancer cell or 4T1 b...

journal_title：Microbiology and immunology

authors： Ma C,Su M,Shen K,Chen J,Ning Y,Qi C

更新日期：2020-01-01 00:00:00

abstract：:Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were prepared and an ass...

journal_title：Microbiology and immunology

authors： Kuramitsu M,Okuma K,Nakashima M,Sato T,Sasaki D,Hasegawa H,Umeki K,Kubota R,Sasada K,Sobata R,Matsumoto C,Kaneko N,Tezuka K,Matsuoka S,Utsunomiya A,Koh KR,Ogata M,Ishitsuka K,Taki M,Nosaka K,Uchimaru K,Iwanaga M

更新日期：2018-10-01 00:00:00

abstract：:Pneumocystis spp. are opportunistic fungal pathogens that are closely associated with severe pneumonia and pulmonary complications in patients with impaired immunity. In this study, the antigenic epitopes of the gene encoding the 55 kDa antigen fragment of Pneumocystis (p55), which may play an important role in Pneumo...

journal_title：Microbiology and immunology

authors： Fan H,Guo JY,Ma SL,Zhang N,An CL

更新日期：2016-06-01 00:00:00

abstract：:We isolated a filamentous bacteriophage, VfO4K68, from the pandemic Vibrio parahaemolyticus strain belonging to 04:K68 serovar. The VfO4K68 DNA lacked a 1,893-bp fragment present in that of the distinctive region of f237, a filamentous phage isolated from a pandemic 03:K6 strain (Nasu, H. et al., J. Clin. Microbiol., ...

journal_title：Microbiology and immunology

authors： Chan B,Miyamoto H,Taniguchi H,Yoshida S

更新日期：2002-01-01 00:00:00

abstract：:Antimicrobial peptides contribute to innate host defense against a number of bacteria and fungal pathogens. Some of antimicrobial synthetic peptides were systemically administered in vivo; however, effective protection has so far not been obtained because the effective dose of peptides in vivo seems to be very high, o...

journal_title：Microbiology and immunology

authors： Hamamoto K,Kida Y,Zhang Y,Shimizu T,Kuwano K

更新日期：2002-01-01 00:00:00

abstract：:The botulinum neurotoxin light chain (BoNT-LC) is a zinc-dependent metalloprotease that cleaves neuronal SNARE proteins such as SNAP-25, VAMP2, and Syntaxin1. This cleavage interferes with the neurotransmitter release of peripheral neurons and results in flaccid paralysis. SNAP, VAMP, and Syntaxin are representative o...

journal_title：Microbiology and immunology

authors： Yamamoto H,Ida T,Tsutsuki H,Mori M,Matsumoto T,Kohda T,Mukamoto M,Goshima N,Kozaki S,Ihara H

更新日期：2012-04-01 00:00:00

abstract：:A mass outbreak of Escherichia coli O157:H45 was first reported in Japan in 1998. This pathogen was classified as an enteropathogenic E. coli (EPEC) O157 because it was characterized by the Shiga toxin gene (stx)-negative and bundle-forming pilus (bfp) gene-positive genotypes. In this study, we investigated the type I...

journal_title：Microbiology and immunology

authors： Abe A,Nagano H

更新日期：2000-01-01 00:00:00

abstract：:Rapid western blot (WB) procedure for an abnormal isoform of prion protein (PrP(Sc) ) detection in lymphoid tissues was established and has been applied to the surveillance of fallen stock. In this program, brain and palatal tonsil were examined by WB and three cases of sheep scrapie were detected. While one clinicall...

journal_title：Microbiology and immunology

authors： Shimada K,Hayashi HK,Ookubo Y,Iwamaru Y,Imamura M,Takata M,Schmerr MJ,Shinagawa M,Yokoyama T

更新日期：2005-01-01 00:00:00

abstract：:A mutant MT of Micrococcus lysodeikticus (luteus) IFO 3333, whose minimum growing unit is not a single cell, but a tetrad unlike the wild-type divides by binary fission of each monococcus, and then separates first into two daughter tetrads, second into four tetrads and third into eight tetrads. The three planes of eit...

journal_title：Microbiology and immunology

authors： Monodane T,Matsushima Y,Kotani S

更新日期：1978-01-01 00:00:00

abstract：:The distribution of the antibody to influenza C virus in the dogs and pigs in Yamagata prefecture, Japan was investigated by using three different serological methods: hemagglutination-inhibition (HI), radioimmunoprecipitation (RIP), and immunoblotting. The antibody against influenza C virus glycoprotein (gp88) was de...

journal_title：Microbiology and immunology

authors： Ohwada K,Kitame F,Sugawara K,Nishimura H,Homma M,Nakamura K

更新日期：1987-01-01 00:00:00

abstract：:A 9.8-kbp DNA fragment which contained a neurotoxin gene and its upstream region was cloned from Clostridium botulinum type D strain CB-16. Nucleotide sequencing of the fragment revealed that genes encoding for hemagglutinin (HA) subcomponents and one for a nontoxic-nonhemagglutinin (NTNH) component were located upstr...

journal_title：Microbiology and immunology

authors： Ohyama T,Watanabe T,Fujinaga Y,Inoue K,Sunagawa H,Fujii N,Inoue K,Oguma K

更新日期：1995-01-01 00:00:00

abstract：:Tetrads-forming mutant MT cells of Micrococcus luteus, both treated with chemical reagents and non-treated, were observed with a scanning electron microscope (SEM). The agglutinability of the cells with antiserum containing anti-teichuronic acid antibody was examined. The binding of protein A-gold particles to the cel...

journal_title：Microbiology and immunology

authors： Monodane T,Tokunaga M,Torii M

更新日期：1990-01-01 00:00:00

abstract：:A reversed passive latex agglutination (RPLA) assay for detecting Clostridium difficile toxin A is presented. Purified monoclonal antibody (mAb 37B5) was used for latex sensitization. The culture supernatants of 93 strains of C. difficile were tested by RPLA assay and the results compared with those of a commercially ...

journal_title：Microbiology and immunology

authors： Toma C,Nakamura S,Kamiya S,Nakasone N,Iwanaga M

更新日期：1999-01-01 00:00:00

abstract：:Mg2+-availability in Staphylococcus aureus cells decreased significantly with increasing NaCl concentration in growth media. Cells grew in a NaCl-free, Chelex resin-treated complex medium only if the medium was supplemented with 50 microM MgCl2, while, growth was limited when the medium was further supplemented with 1...

journal_title：Microbiology and immunology

authors： Ochiai T

更新日期：2001-01-01 00:00:00

abstract：:Pathogens of the Aspergillus species are frequently seen in deep-seated mycoses. We previously demonstrated that the culture filtrate of Aspergillus fumigatus (CF) has immunosuppressive effects on polymorphonuclear leukocytes (PMNs), which act as the main phagocytes to hyphae of Aspergillus fumigatus (A. fumigatus). B...

journal_title：Microbiology and immunology

authors： Higurashi H,Arai M,Watanabe A,Igari H,Seki N,Kamei K,Kuriyama T

更新日期：2007-01-01 00:00:00

abstract：:To clarify the humoral immunity in herpes simplex virus (HSV) infection, HSV-specific IgM, IgA and IgG subclass antibody responses were studied in patients with genital herpes: 17 primary, 13 recurrent and 6 nonprimary first episode. A total of 181 serum samples serially collected from the patients, 5 per patient unti...

journal_title：Microbiology and immunology

authors： Hashido M,Kawana T

更新日期：1997-01-01 00:00:00