Bactericidal effect of fatty acids on mycobacteria, with particular reference to the suggested mechanism of intracellular killing.

abstract：:The phospholipase C (alpha-toxin) gene (plc) of Clostridium perfringens was cloned into pUC19 and the effects of the upstream regions on expression of the plc gene were examined in Escherichia coli JM109. When the 0.7-kb region just upstream of the putative -35 site of the gene was deleted, production of phospholipase...

journal_title：Microbiology and immunology

authors： Toyonaga T,Matsushita O,Katayama S,Minami J,Okabe A

更新日期：1992-01-01 00:00:00

abstract：:Twelve hybridoma cell lines producing monoclonal antibodies to the human interleukin 2 (IL-2) receptor (IL-2R) molecule were prepared. These antibodies were characterized by competitive antibody-binding assay and sequential immunoprecipitation assay with four known monoclonal antibodies to the human IL-2R molecule. Th...

journal_title：Microbiology and immunology

authors： Tanaka Y,Inoi T,Tozawa H,Sugamura K,Hinuma Y

更新日期：1986-01-01 00:00:00

abstract：:Irradiation treatment enhanced resistance of C57BL/6, but not BALB/c against Toxoplasma gondii infection. Six Gy-irradiated (IR) C57BL/6 recipients of B-2 cells from T. gondii-infected C57BL/6 died after infection. B-2 suppressor cells from infected C57BL/6 enhanced production of IL-4 and IL-10 in peritoneal exudate c...

journal_title：Microbiology and immunology

authors： Mun HS,Aosai F,Fang H,Piao LX,Winn T,Norose K,Yano A

更新日期：2005-01-01 00:00:00

abstract：:The present study was designed to evaluate the distribution of epithelioid cells, myofibroblasts, and TGF-beta1 in the formation of granuloma caused by Mycobacterium avium intracellulare complex (MAC) lung infection. A retrospective study was performed for 9 cases of positive MAC culture in which lung resections were ...

journal_title：Microbiology and immunology

authors： Fujita J,Ohtsuki Y,Suemitsu I,Yamadori I,Shigeto E,Shiode M,Nishimura K,Hirayama T,Matsushima T,Ishida T

更新日期：2002-01-01 00:00:00

abstract：:Sialyl Lewis X (SLe(x)), an E-selectin ligand, was conjugated with carboxymethylpullulan (CMPul) and the disposition characteristics of this conjugate after intravenous administration were investigated using mice with ear edema. The concentration of 3H-labeled SLe(x)-CMPul in the spleen was significantly high. When CM...

journal_title：Microbiology and immunology

authors： Horie K,Sakagami M,Kuramochi K,Ito T,Hamana H

更新日期：2000-01-01 00:00:00

abstract：:A serological typing scheme of Bacillus cereus has been developed by immunochemical analyses of flagellar antigen using an agglutination method. Enzyme-linked immunosorbent assay (ELISA) for the classification of flagellar serotype of Bacillus cereus had greater sensitivity. 10-500 times, than that of agglutination me...

journal_title：Microbiology and immunology

authors： Murakami T,Hiraoka K,Mikami T,Matsumoto T,Katagiri S,Suzuki M

更新日期：1991-01-01 00:00:00

abstract：:Recurrent otitis media are frequently intractable during childhood. It is unclear whether recurrent otitis media is caused by etiological bacteria colonization or by new infections. Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis were isolated from the nasopharynx of 7 otitisprone and 2 non-...

journal_title：Microbiology and immunology

authors： Yokota S,Harimaya A,Sato K,Somekawa Y,Himi T,Fujii N

更新日期：2007-01-01 00:00:00

abstract：:Photodynamic therapy (PDT) has been considered a feasible alternative for antimicrobial therapy of multidrug-resistant pathogens. However, bacterial response mechanisms against PDT-generated photo-oxidative stress remain largely unknown. Herein, it is shown that the accessory gene regulator Agr is involved in Staphylo...

journal_title：Microbiology and immunology

authors： Park HJ,Moon YH,Yoon HE,Park YM,Yoon JH,Bang IS

更新日期：2013-08-01 00:00:00

abstract：:Common EDTA-soluble antigens of Clostridium chauvoei and C. septicum were examined by indirect-immunofluorescence (IFA) and immunoblot analysis. The monoclonal antibodies (mAbs) specific for the 35 kDa antigen of C. chauvoei strain ATCC 10092 were used. These mAbs reacted with all 11 strains, 6 of C. chauvoei and 5 of...

journal_title：Microbiology and immunology

authors： Hamaoka T,Terakado N,Nakamura S

更新日期：1994-01-01 00:00:00

abstract：:The botulinum neurotoxin light chain (BoNT-LC) is a zinc-dependent metalloprotease that cleaves neuronal SNARE proteins such as SNAP-25, VAMP2, and Syntaxin1. This cleavage interferes with the neurotransmitter release of peripheral neurons and results in flaccid paralysis. SNAP, VAMP, and Syntaxin are representative o...

journal_title：Microbiology and immunology

authors： Yamamoto H,Ida T,Tsutsuki H,Mori M,Matsumoto T,Kohda T,Mukamoto M,Goshima N,Kozaki S,Ihara H

更新日期：2012-04-01 00:00:00

abstract：:The 16S rRNA gene sequences were determined for type strains of 21 Bifidobacterium species. A phylogenetic tree was constructed using the determined sequences and sequences from DNA databases, which contain the sequences of 11 type strains of Bifidobacterium species and 11 strains of related genera. All species of the...

journal_title：Microbiology and immunology

authors： Miyake T,Watanabe K,Watanabe T,Oyaizu H

更新日期：1998-01-01 00:00:00

abstract：:The antibacterial activity of cefpodoxime against Branhamella catarrhalis was studied. All of the 65 clinical isolates tested were inhibited at and below 1.56 micrograms/ml, both at 10(7) and at 10(5) CFUs. The following was further studied on B. catarrhalis N-5 which showed average susceptibility to each drug examine...

journal_title：Microbiology and immunology

authors： Takenouchi T,Nishino T

更新日期：1991-01-01 00:00:00

abstract：:We developed a rapid and simple method for the screening of antiviral agents against herpes simplex virus (HSV) in a model of gastrointestinal herpetic infection in vitro. This method was based on inhibition of HSV-induced cytopathogenicity in gastric adenocarcinoma MKN-28 cells, as monitored by an MTT colorimetric as...

journal_title：Microbiology and immunology

authors： Kodama E,Igarashi A,Mori S,Hashimoto K,Suzuki T,De Clercq E,Shigeta S

更新日期：1996-01-01 00:00:00

abstract：:Site-specific recombinase is widely applied for the regulation of gene expression because its regulatory action is strict and efficient. However, each system can mediate regulation of only one gene at a time. Here, we demonstrate efficient "sequential" gene regulation using Cre-and FLP-expressing recombinant adenoviru...

journal_title：Microbiology and immunology

authors： Kondo S,Takahashi Y,Shiozawa S,Ichise H,Yoshida N,Kanegae Y,Saito I

更新日期：2006-01-01 00:00:00

abstract：:The nucleotide sequence of coxsackievirus B6 (CVB6) has been determined, and the nucleotides encoding the 5' nontranslated region (5' NTR) and virion polypeptides (VP4, 2, 3 and 1) were compared with other serotype CVBs. An Unweighted Pair-Group Method Analysis (UPGMA) of phylogenetic trees indicated that the 5' NTR o...

journal_title：Microbiology and immunology

authors： Kato S,Tsutsumi R,Sato S

更新日期：1999-01-01 00:00:00

abstract：:To clarify the epidemiology of viral acute respiratory infections (ARIs), 305 human parainfluenza virus types 1 (HPIV1), 154 HPIV2 and 574 HPIV3 strains were isolated from 16,962 nasopharyngeal swabs obtained between 2002 and 2011 at pediatric clinics in Yamagata, Japan. The total isolation frequency for HPIV1-3 was 6...

journal_title：Microbiology and immunology

authors： Mizuta K,Abiko C,Aoki Y,Ikeda T,Itagaki T,Katsushima F,Katsushima Y,Matsuzaki Y,Noda M,Kimura H,Ahiko T

更新日期：2012-12-01 00:00:00

abstract：:Polar monotrichous flagella (M-flagella) of Vibrio parahaemolyticus have antigens in common with those of various species of Vibrio including V. cholerae and V. anguillarum, and of Beneckea, revealed by gel diffusion tests with flagelli monomers. However, antiserum against M-flagellin of V. parahaemolyticus did not ag...

journal_title：Microbiology and immunology

authors： Shinoda S,Senoh T,Asano K,Nakahara N,Ono B

更新日期：1980-01-01 00:00:00

abstract：:pTK159, a multiresistance 40-kilobase (kb) plasmid, was isolated from a clinical strain of Serratia marcescens. pTK159 was nonconjugative and carried determinants for resistance to amikacin, streptomycin/spectinomycin, sulfamethoxazole and ampicillin. A physical and functional map of pTK159 was constructed. By cloning...

journal_title：Microbiology and immunology

authors： Kanno R

更新日期：1988-01-01 00:00:00

abstract：:Fifty-eight enterotoxigenic Escherichia coli (ETEC) strains, isolated from children with and without diarrhea in São Paulo, were examined for the presence of colonization factor antigens (CFAs) and their ability to adhere to HeLa cells. Antisera to CFA/I, the coli surface (CS) antigens CS1CS3, CS2CS3, and CS2 of CFA/I...

journal_title：Microbiology and immunology

authors： Guth BE,Aguiar EG,Griffin PM,Ramos SR,Gomes TA

更新日期：1994-01-01 00:00:00

abstract：:Cross-linking of surface receptors in hematopoietic cells results in the enrichment of these receptors in the rafts along with other downstream signaling molecules. A possible explanation how signal is transduced through the plasma membrane has arisen from the concept of raft. From the study of cellular responses in t...

journal_title：Microbiology and immunology

authors： Katagiri YU,Kiyokawa N,Fujimoto J

更新日期：2001-01-01 00:00:00

abstract：:Antibodies specific to Salmonella enteritidis (S.E.) were obtained from immunized egg yolk, and their protective effects against S.E. were studied by using monolayer-cultured human intestinal epithelial cells, Caco-2 and T84. The Salmonella adherence and entry to the cells were partially inhibited by the antibodies. T...

journal_title：Microbiology and immunology

authors： Sugita-Konishi Y,Ogawa M,Arai S,Kumagai S,Igimi S,Shimizu M

更新日期：2000-01-01 00:00:00

abstract：:Clostridium perfringens KZ1340, previously classified as Clostridium plagarum, is an isolate from Antarctic soil, and was identified as an alpha-, theta-, and kappa-toxin non-producing variant. On Southern hybridization, the variant was found to be defective in the pfoA (theta-toxin) gene, but the plc (alpha-toxin) an...

journal_title：Microbiology and immunology

authors： Shimizu T,Ohtani K,Ba-Thein W,Inui S,Nakamura S,Hayashi H

更新日期：1996-01-01 00:00:00

abstract：:Selective and differential media were designed for each species of Pityrosporum; P. pachydermatis, P. ovale, and P. orbiculare in order to make feasible a quantitative cultivation. Medium for P. pachydermatis (medium A) was composed of 1% trypticase peptone (BBL), 0.5% yeast extract (BBL), 0.3% glucose, 0.2% NaCl, 1.2...

journal_title：Microbiology and immunology

authors： Ushijima T,Takahashi M,Ozaki Y

更新日期：1981-01-01 00:00:00

abstract：:Soil specimens obtained from several areas of Japan, which are closely located to or facing the Continental land of China, were examined for the distribution of Clostridium botulinum, especially pertaining to types A and B. A total of 266 specimens of Japan, when cultured, showed no type A or B toxicity, although 30 (...

journal_title：Microbiology and immunology

authors： Yamakawa K,Kamiya S,Nishida S,Yoshimura K,Yu H,Lu DY,Nakamura S

更新日期：1988-01-01 00:00:00

abstract：:We developed the antigen capture enzyme-linked immunosorbent assay (ELISA) systems for quantification of Borna disease virus (BDV) major antigens, p40 and p24. Using these ELISAs, we quantified the two proteins in various BDV-infected materials, including the cell lysates and culture supernatants as well as the homoge...

journal_title：Microbiology and immunology

authors： Watanabe M,Zhong Q,Kobayashi T,Kamitani W,Tomonaga K,Ikuta K

更新日期：2000-01-01 00:00:00

abstract：:Live attenuated bacteria can be used as a carrier for the delivery of foreign antigens to a host's immune system. The N-terminal domain of SipB, a translocon protein of the type III secretion system of Salmonella enterica serovar Typhimurium, is required for secretion and outer membrane localization. In the present st...

journal_title：Microbiology and immunology

authors： Jang JI,Kim JS,Eom JS,Kim HG,Kim BH,Lim S,Bang IS,Park YK

更新日期：2012-09-01 00:00:00

abstract：:Influenza A virus (IAV) PA-X is a critical ribonuclease protein involved in host cell shutoff but its role in modulating the host immune response to IAV infection remains to be addressed. In this study, host cellular proteins that directly interact with PA-X were screened to investigate the biological function of PA-X...

journal_title：Microbiology and immunology

authors： Li M,Qi W,Chang Q,Chen R,Zhen D,Liao M,Wen J,Deng Y

更新日期：2020-11-26 00:00:00

abstract：:Natural killer cells, a critical component of the innate immune system, eradicate both virus-infected cells and tumor cells through cytotoxicity and secretion of cytokines. Human NK cell research has largely been based on in vitro studies because of the lack of appropriate animal models. In this study, a selective pro...

journal_title：Microbiology and immunology

authors： Hattori S,Matsuda K,Kariya R,Harada H,Okada S

更新日期：2016-02-01 00:00:00

abstract：:The effects of B subunit of cholera toxin (CTB) on delayed-type hypersensitivity (DTH) response to influenza vaccine derived from influenza virus A/PR/8/34 (PR-8, HlNl) virus were investigated in B10 mice that were immunized intranasally with both influenza vaccine and CTB. The result showed that intranasal inoculatio...

journal_title：Microbiology and immunology

authors： Kikuta K,Kurata H,Nagamine T,Aizawa C,Ueno Y,Kurata T,Tamura S

更新日期：1990-01-01 00:00:00

abstract：:Germfree (GF) F344 rats developed severe adjuvant-induced arthritis with a 100% incidence after a single intradermal injection of heat-killed Mycobacterium bovis (BCG). Specific pathogene-free (SPF) rats developed less severe arthritis with a lower incidence. The rats colonized with Escherichia coli or Bacteroides dev...

journal_title：Microbiology and immunology

authors： Kohashi O,Kohashi Y,Takahashi T,Ozawa A,Shigematsu N

更新日期：1985-01-01 00:00:00