Abstract:
:Pneumocystis spp. are opportunistic fungal pathogens that are closely associated with severe pneumonia and pulmonary complications in patients with impaired immunity. In this study, the antigenic epitopes of the gene encoding the 55 kDa antigen fragment of Pneumocystis (p55), which may play an important role in Pneumocystis pneumonia, were analyzed. A gene containing tandem variants of the p55 antigen was synthesized and named the tandem antigen gene (TAG). TAG's potential as a DNA vaccine was assessed in immunosuppressed rats. Immunization with p55-TAG DNA vaccine significantly reduced both the pathogen burden and lung-weight to body-weight ratios. Additionally, p55-TAG vaccination in immunosuppressed rats elicited both cell-mediated and humoral immunity.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Fan H,Guo JY,Ma SL,Zhang N,An CLdoi
10.1111/1348-0421.12386subject
Has Abstractpub_date
2016-06-01 00:00:00pages
397-406issue
6eissn
0385-5600issn
1348-0421journal_volume
60pub_type
杂志文章abstract::Antiplague indirect hemagglutination tests were investigated with Yersinia pestis fraction-I antigen and sera from experimentally infected rodents. The same tests with sera treated with acetone or acetone-2-mercaptoethanol were performed in parallel. Technical conditions were determined to insure the specificity and r...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1979.tb00548.x
更新日期:1979-01-01 00:00:00
abstract::Serum samples were collected from 938 pigs of 24 farms in Hokkaido, Kagoshima, and Okinawa prefectures in Japan in 2001-2005. Enzyme-linked immunosorbent assay (ELISA) was used for the detection of antibodies to LipL32 antigen which is common to Leptospira interrogans. Samples positive in ELISA were then investigated ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2007.tb03945.x
更新日期:2007-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1994.tb01823.x
更新日期:1994-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2002.tb02677.x
更新日期:2002-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2003-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1998.tb02330.x
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journal_title:Microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1348-0421.2008.00028.x
更新日期:2008-02-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2005.tb03728.x
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1995.tb02206.x
更新日期:1995-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1996.tb01138.x
更新日期:1996-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2011.00347.x
更新日期:2011-07-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1997.tb01944.x
更新日期:1997-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1997.tb01954.x
更新日期:1997-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12470
更新日期:2017-02-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1994.tb01754.x
更新日期:1994-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2011.00329.x
更新日期:2011-06-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1995.tb02218.x
更新日期:1995-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1979.tb00491.x
更新日期:1979-01-01 00:00:00
abstract::A loop-mediated isothermal amplification (LAMP) assay for simple detection of Bordetella holmesii was developed. This assay discriminates between B. holmesii and other Bordetella species and successfully detect B. holmesii DNA in nasopharyngeal swab samples from subjects with suspected pertussis. The LAMP assay result...
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pub_type: 杂志文章
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1980.tb02845.x
更新日期:1980-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2008.00004.x
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1982.tb00156.x
更新日期:1982-01-01 00:00:00
abstract::β-1,3-glucan is a major component of fungal cell walls with various biological activities, including effects on the production of inflammatory mediators in vivo and in vitro. However, few reports have examined its influence on the fungal cell itself. In this study, the influences of β-1,3-glucan on the growth and cell...
journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2010-11-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1991.tb01569.x
更新日期:1991-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2005.tb03645.x
更新日期:2005-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1997.tb01183.x
更新日期:1997-01-01 00:00:00
abstract::The immune responses of individuals exposed to Leishmania major were evaluated and compared with those of non-exposed volunteers. Forty-one patients with active lesion(s), 43 healed individuals, 15 vaccinees 1 month or 1 year post vaccination, and 15 non-exposed volunteers were studied. Leishmanin skin test (LST) resp...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2007.tb03984.x
更新日期:2007-01-01 00:00:00
abstract::We attempted to detect Coxiella burnetii in dust samples collected from a barn housing dairy cattle by the polymerase chain reaction (PCR) method. Ten dust samples (five from ventilation fans and five from crossbeams) were collected from two areas in a barn on a farm near Sapporo, Hokkaido. C. burnetii was detected in...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1998.tb01969.x
更新日期:1998-01-01 00:00:00
abstract::Japanese encephalitis virus (JEV) core protein is detected not only in the cytoplasm but also in the nucleoli of infected cells. We previously showed that a mutant JEV lacking the nucleolar localization of the core protein impaired viral replication in mammalian cells. In this study, we identified a nucleolar phosphop...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2006.tb03789.x
更新日期:2006-01-01 00:00:00
abstract::Antibodies to fibronectin-binding proteins (FnBPs) of Staphylococcus aureus, including binding domain of FnBPA, the D region, or the A-C regions of FnBPB were produced in rabbits and mice. These antibodies were used to characterize cell-associated FnBPs of S. aureus strain Cowan I, S. aureus strain U320 and a coagulas...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1994.tb01791.x
更新日期:1994-01-01 00:00:00