Abstract:
:Alkaline phosphatase-conjugated oligonucleotide probes were developed to detect the gene coding for Vero toxin 1 (VT1) and Vero toxin 2 (VT2). Using these probes, 3 hr was enough to detect VT genes when suspicious colonies of enterohaemorrhagic Escherichia coli (EHEC) were obtained on an agar plate. The results of a hybridization test with 144 isolates of EHEC O157 and one isolate of Shigella dysenteriae Type 1 agreed exactly with the immunological detection, reversed passive latex agglutination (RPLA) test, of VTs in their culture supernatants. The sensitivity levels of these probes for the detection of VT genes were 100%. The specificity of these probes were also tested with a total of 1,002 strains of Escherichia coli other than EHEC and 8 strains of Shigella sp. other than Shigella dysenteriae Type 1; the results showed 100% specificity.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Yoh M,Takagi K,Eda J,Ohtomo M,Takarada Y,Shibata S,Honda Tdoi
10.1111/j.1348-0421.1997.tb01944.xsubject
Has Abstractpub_date
1997-01-01 00:00:00pages
879-82issue
11eissn
0385-5600issn
1348-0421journal_volume
41pub_type
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