Effect of cytokine-encoding plasmid delivery on immune response to Japanese encephalitis virus DNA vaccine in mice.

Abstract:

:We have previously shown that immunization of mice with plasmid pMEa synthesizing Japanese encephalitis virus (JEV) envelope protein induced anti-JEV humoral and cellular immune responses. We now show that intra-muscular co-administration of mice with pMEa and pGM-CSF, encoding murine granulocyte-macrophage colony-stimulating factor or pIL-2, encoding murine interleukin-2 given 4 days after pMEa, augmented anti-JEV antibody titers. This did not enhance the level of protection in immunized mice against JEV. However, intra-dermal co-administration of pMEa and pGM-CSF in mice using the gene gun, enhanced anti-JEV antibody titers resulting in an increased level of protection in mice against lethal JEV challenge.

journal_name

Microbiol Immunol

authors

Bharati K,Appaiahgari MB,Vrati S

doi

10.1111/j.1348-0421.2005.tb03739.x

keywords:

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

349-53

issue

4

eissn

0385-5600

issn

1348-0421

pii

JST.JSTAGE/mandi/49.349

journal_volume

49

pub_type

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