Effect of constitutively expressed phoP gene on the localization of Salmonella typhimurium within Mac-1 positive phagocytes.

abstract：:The spontaneous production of a rat C-type RNA virus (ACV) in a cultured cell line (AC cells) established from a chemically induced rat glioma was studied. The characteristics of ACV were: morphology typical of C-type RNA virus; buoyant density of 1.15 g/ml in a sucrose density gradient; RNA directed DNA polymerase ac...

journal_title：Microbiology and immunology

authors： Igarashi K,Sasada R,Niiyama Y,Kozai Y,Sugino Y

更新日期：1979-01-01 00:00:00

abstract：:Viable but nonculturable (VBNC) Vibrio cholerae non-O1/non-O139, V. parahaemolyticus, enterohemorrhagic Escherichia coli, enterotoxigenic E. coli, enteropathogenic E. coli, Shigella flexneri, and Salmonella enterica were converted to the culturable state by co-culture with selected eukaryotic cells, e.g., HT-29, Caco-...

journal_title：Microbiology and immunology

authors： Senoh M,Ghosh-Banerjee J,Ramamurthy T,Colwell RR,Miyoshi S,Nair GB,Takeda Y

更新日期：2012-05-01 00:00:00

abstract：:Macrophages act as a reservoir for Mycobacterium tuberculosis, producing latent infection in approximately 90% of infected people. In this study, J774A.1 mouse macrophage cell line response and microRNA (miRNA) expression during infection with the most relevant mycobacterial strains for humans (M. tuberculosis, M. bov...

journal_title：Microbiology and immunology

authors： Elizabeth MC,Hernández de la Cruz ON,Mauricio CA

更新日期：2016-05-01 00:00:00

abstract：:Inhibition of hemagglutinin (HA) activity in a membrane fraction of Bacteroides gingivalis was examined using various compounds. Leupeptin and anti-pain inhibited the HA activity at nM order. This potency was lost when the aldehyde group of leupeptin was converted to an alcohol moiety. Irreversible protease inhibitors...

journal_title：Microbiology and immunology

authors： Nishikata M,Yoshimura F,Nodasaka Y

更新日期：1989-01-01 00:00:00

abstract：:Although the RC-HL strain of rabies virus is avirulent in adult mice, the amino acid at position 333 of its G protein is arginine, which is thought to be necessary for virulence in adult mice upon intracerebral inoculation of the virus. This result suggests that besides arginine at position 333, some other positions o...

journal_title：Microbiology and immunology

authors： Ito H,Minamoto N,Watanabe T,Goto H,Rong LT,Sugiyama M,Kinjo T,Mannen K,Mifune K,Konobe T

更新日期：1994-01-01 00:00:00

abstract：:Stromal cell-derived factor-1 (SDF-1) is an efficacious chemoattractant for lymphocytes, monocytes and hematopoietic progenitor cells. In the present study, we examined whether SDF-1 has growth promoting activity on human peripheral T cells and analyzed the possible underlying signal transduction pathways. SDF-1 augme...

journal_title：Microbiology and immunology

authors： Yonezawa A,Hori T,Sakaida H,Uchiyama T

更新日期：2000-01-01 00:00:00

abstract：:We compared the intracellular survival and growth of Legionella pneumophila Philadelphia-1 in peritoneal macrophages obtained from A/J, C57BL/6, and X-linked chronic granulomatous disease (CGD) mice produced from C57BL/6 strain. The initial killing was observed in A/J and C57BL/6 macrophages at 2, 4 and 6 hr after in ...

journal_title：Microbiology and immunology

authors： Saito M,Kajiwara H,Miyamoto H,Yoshida S

更新日期：2001-01-01 00:00:00

abstract：:Antibodies specific to Salmonella enteritidis (S.E.) were obtained from immunized egg yolk, and their protective effects against S.E. were studied by using monolayer-cultured human intestinal epithelial cells, Caco-2 and T84. The Salmonella adherence and entry to the cells were partially inhibited by the antibodies. T...

journal_title：Microbiology and immunology

authors： Sugita-Konishi Y,Ogawa M,Arai S,Kumagai S,Igimi S,Shimizu M

更新日期：2000-01-01 00:00:00

abstract：:An emetic toxin cereulide, produced by Bacillus cereus, causes emetic food poisonings, but a method for quantitative measurement of cereulide has not been well established. A current detection method is a bioassay method using the HEp-2 cell vacuolation test, but it was unable to measure an accurate concentration. We ...

journal_title：Microbiology and immunology

authors： Kawamura-Sato K,Hirama Y,Agata N,Ito H,Torii K,Takeno A,Hasegawa T,Shimomura Y,Ohta M

更新日期：2005-01-01 00:00:00

abstract：:The plasmids R401 and Rtsl belong to the same incompatibility group, IncT. The nucleotide sequence of the basic replicon of R401 consisting of 1,857 base pairs was determined and compared with that of mini-Rtsl previously reported. The mini-R401 was found to be composed of two clusters of direct repeated sequences fla...

journal_title：Microbiology and immunology

authors： Tabuchi A

更新日期：1985-01-01 00:00:00

abstract：:Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were prepared and an ass...

journal_title：Microbiology and immunology

authors： Kuramitsu M,Okuma K,Nakashima M,Sato T,Sasaki D,Hasegawa H,Umeki K,Kubota R,Sasada K,Sobata R,Matsumoto C,Kaneko N,Tezuka K,Matsuoka S,Utsunomiya A,Koh KR,Ogata M,Ishitsuka K,Taki M,Nosaka K,Uchimaru K,Iwanaga M

更新日期：2018-10-01 00:00:00

abstract：:Streptococcus suis infection induces formation of neutrophil extracellular traps (NETs) in vitro; however, the contribution of NETs-mediated killing to the pathogenesis of S. suis in vivo is yet to be elicited. The findings of the present study indicated that extracellular DNA fiber can be induced in a murine model in...

journal_title：Microbiology and immunology

authors： Zhao J,Lin L,Fu L,Han L,Zhang A

更新日期：2016-04-01 00:00:00

abstract：:Recipient cells migrating into the transplantation site of an allograft recognize histocompatibility antigens on the grafts and are cytotoxic against the grafts. Although the alloreactive immune response is predominantly directed at the major histocompatibility complex (major histocompatibility complex [MHC]; H-2 in m...

journal_title：Microbiology and immunology

authors： Inoue Y,Tashiro-Yamaji J,Hayashi M,Kiyonari H,Shimizu T,Ibata M,Yamana H,Kubota T,Tanigawa N,Yoshida R

更新日期：2011-06-01 00:00:00

abstract：:Cattle enterotoxemia caused by Clostridium perfringens toxins is a noncontagious, sporadic, and fatal disease characterized by sudden death. Strategies for controlling and preventing cattle enterotoxemia are based on systematic vaccination of herds with toxoids. Because the process of producing conventional clostridia...

journal_title：Microbiology and immunology

authors： Jiang Z,De Y,Chang J,Wang F,Yu L

更新日期：2014-11-01 00:00:00

abstract：:In recent years, phosphatidylinositol 4-kinase III beta (PI4KB) has emerged as a conserved target of anti-picornavirus compounds. In the present study, PI4KB was identified as the direct target of the plant-derived anti-picornavirus compounds, oxoglaucine and pachypodol (also known as Ro 09-0179). PI4KB was also ident...

journal_title：Microbiology and immunology

authors： Arita M,Philipov S,Galabov AS

更新日期：2015-06-01 00:00:00

abstract：:Common EDTA-soluble antigens of Clostridium chauvoei and C. septicum were examined by indirect-immunofluorescence (IFA) and immunoblot analysis. The monoclonal antibodies (mAbs) specific for the 35 kDa antigen of C. chauvoei strain ATCC 10092 were used. These mAbs reacted with all 11 strains, 6 of C. chauvoei and 5 of...

journal_title：Microbiology and immunology

authors： Hamaoka T,Terakado N,Nakamura S

更新日期：1994-01-01 00:00:00

abstract：:The attachment of Mycoplasma pulmonis m53 organisms to mouse and rat synovial cells was examined by using the organisms and the synovial cells treated in various ways. M. pulmonis treated with trypsin attached to the synovial cells, but the organisms treated with pronase, formaldehyde, glutaraldehyde, or heat did not....

journal_title：Microbiology and immunology

authors： Araake M,Yayoshi M,Yoshioka M

更新日期：1985-01-01 00:00:00

abstract：:A total of 55 strains of Clostridium sordellii, 21 lethal toxin-positive and 34 lethal toxin-negative, were tested for cytotoxin production in brain heart infusion medium supplemented with 0.2% Na2HPO4 (m-BHI) and cooked-meat-glucose (CMG) medium using baby hamster kidney (BHK-21/WI-2) cells as indicator cells. The m-...

journal_title：Microbiology and immunology

authors： Nakamura S,Tanabe N,Yamakawa K,Nishida S

更新日期：1983-01-01 00:00:00

abstract：:A 9.8-kbp DNA fragment which contained a neurotoxin gene and its upstream region was cloned from Clostridium botulinum type D strain CB-16. Nucleotide sequencing of the fragment revealed that genes encoding for hemagglutinin (HA) subcomponents and one for a nontoxic-nonhemagglutinin (NTNH) component were located upstr...

journal_title：Microbiology and immunology

authors： Ohyama T,Watanabe T,Fujinaga Y,Inoue K,Sunagawa H,Fujii N,Inoue K,Oguma K

更新日期：1995-01-01 00:00:00

abstract：:DNase B is a major nuclease and a possible virulence factor in Streptococcus pyogenes. The allelic diversity of streptococcal DNase B (sdaB) gene was investigated in 83 strains with 14 emm genotypes. Of the 15 alleles identified, 11 alleles carried only synonymous nucleotide substitutions. On the other hand, 4 alleles...

journal_title：Microbiology and immunology

authors： Matsumoto M,Sakae K,Hashikawa S,Torii K,Hasegawa T,Horii T,Endo M,Okuno R,Murayama S,Hirasawa K,Suzuki R,Isobe J,Tanaka D,Katsukawa C,Tamaru A,Tomita M,Ogata K,Ikebe T,Watanabe H,Ohta M,Working Group for Group A S

更新日期：2005-01-01 00:00:00

abstract：:We developed the antigen capture enzyme-linked immunosorbent assay (ELISA) systems for quantification of Borna disease virus (BDV) major antigens, p40 and p24. Using these ELISAs, we quantified the two proteins in various BDV-infected materials, including the cell lysates and culture supernatants as well as the homoge...

journal_title：Microbiology and immunology

authors： Watanabe M,Zhong Q,Kobayashi T,Kamitani W,Tomonaga K,Ikuta K

更新日期：2000-01-01 00:00:00

abstract：:Many pathogens colonize host tissues by binding to the extracellular matrix via their cell surface adhesion molecules, which are called MSCRAMMs (microbial surface components recognizing adhesive matrix molecules). Staphylococcus aureus expresses several of these adhesion molecules, some of which bind to fibronectin. ...

journal_title：Microbiology and immunology

authors： Shinji H,Kamada M,Seki K,Tajima A,Iwase T,Masuda S

更新日期：2007-01-01 00:00:00

abstract：:The mechanism of MHC-restricted T and B cell interactions in antibody response was studied with IgM-inducible B hybridomas and antigen-specific helper T cell clones. B hybridomas were prepared by fusion between splenic B cells from (CBA/N (H-2k) X BALB/c (H-2d)) F1 (NBF1) male mice and a B lymphoma cell line, M12.4.5....

journal_title：Microbiology and immunology

authors： Yoshikubo T,Kimoto M,Kishimoto T

更新日期：1985-01-01 00:00:00

abstract：:Biofilm formation is an important phenotype associated with chronic Pseudomonas aeruginosa infections. In the present study, a total of 48 P. aeruginosa strains isolated from clinical specimens were examined for their biofilm-forming ability using a microtiter plate method. The different biofilm-forming abilities were...

journal_title：Microbiology and immunology

authors： Yang W,Shi L,Jia WX,Yin X,Su JY,Kou Y,Yi X,Shinoda S,Miyoshi S

更新日期：2005-01-01 00:00:00

abstract：:Japanese Lyme borrelias classified as ribotype IV is predominant among isolates derived from clinical specimens, reservoir rodents and Ixodes persulcatus ticks, and has been characterized as Borrelia garinii. These B. garinii isolates have antigenic and genetic features apparently different from North American, Europe...

journal_title：Microbiology and immunology

authors： Wang J,Masuzawa T,Komikado T,Yanagihara Y

更新日期：1997-01-01 00:00:00

abstract：:The effect of dermonecrotic toxin (DNT), fimbrial hemagglutinin (FHA), K-agglutinogen, lipopolysaccharide (LPS), and pertussigen from Bordetella pertussis on the production of IgE and IgG1 antibodies to hen egg albumin (Ea) was investigated in C57BL/6 mice. The IgE antibody contents were determined by passive cutaneou...

journal_title：Microbiology and immunology

authors： Sekiya K

更新日期：1983-01-01 00:00:00

abstract：:This study proposes a novel chemiluminescent assay of bacterial activity. Luminol chemiluminescence (LC) was amplified on addition of menadione to Escherichia coli suspension, and it was effectively inhibited by addition of superoxide dismutase rather than catalase. This fact suggests that H2O2 produced from O2 by sup...

journal_title：Microbiology and immunology

authors： Yamashoji S,Manome I,Ikedo M

更新日期：2001-01-01 00:00:00

abstract：:Enterohaemorrhagic Escherichia coli O157: H7 is an important pathogen these days. Outbreaks of its infection have been reported all over the world, in Australia, Canada, Japan, the United States, south Africa, and various countries in Europe. In the summer of 2001, the first clinical infection by E. coli O157: H7 was ...

journal_title：Microbiology and immunology

authors： Tsai TY,Luo WC,Wu FT,Pan TM

更新日期：2005-01-01 00:00:00

abstract：:Hepatitis C virus (HCV) non-structural protein 5B (NS5B) is an RNA replicase. We expressed full-length NS5B (591 amino acid residues) in Escherichia coli as a fusion protein with maltose binding protein (MBP-NS5B). MBP-NS5B was recovered in the soluble fraction after centrifugation at 40,000 x g and affinity-purified ...

journal_title：Microbiology and immunology

authors： Tanaka T,Sugiyama K,Ikeda M,Naganuma A,Nozaki A,Saito M,Shimotohno K,Kato N

更新日期：2000-01-01 00:00:00

abstract：:Antimicrobial peptides contribute to innate host defense against a number of bacteria and fungal pathogens. Some of antimicrobial synthetic peptides were systemically administered in vivo; however, effective protection has so far not been obtained because the effective dose of peptides in vivo seems to be very high, o...

journal_title：Microbiology and immunology

authors： Hamamoto K,Kida Y,Zhang Y,Shimizu T,Kuwano K

更新日期：2002-01-01 00:00:00