Possibility of Bacteroides gingivalis hemagglutinin possessing protease activity revealed by inhibition studies.

Abstract:

:Inhibition of hemagglutinin (HA) activity in a membrane fraction of Bacteroides gingivalis was examined using various compounds. Leupeptin and anti-pain inhibited the HA activity at nM order. This potency was lost when the aldehyde group of leupeptin was converted to an alcohol moiety. Irreversible protease inhibitors, tosyl-L-lysine chloromethyl ketone (TLCK), p-chloromercuribenzoate (PCMB), and N-ethylmaleimide (NEM) were also inhibitory. From the inhibition experiments, we speculate that the HA possesses protease activity and that the same site of the molecule participates in the erythrocyte binding and the substrate binding.

journal_name

Microbiol Immunol

authors

Nishikata M,Yoshimura F,Nodasaka Y

doi

10.1111/j.1348-0421.1989.tb01499.x

subject

Has Abstract

pub_date

1989-01-01 00:00:00

pages

75-80

issue

1

eissn

0385-5600

issn

1348-0421

journal_volume

33

pub_type

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