Identification of beta-tubulin isoform V as an autoantigen in allergic rhinitis by a proteomic approach.

Abstract:

:Autoantibodies to IgE and beta2-adrenergic receptor have been reported in patients with allergic rhinitis. To investigate whether autoimmunity in allergic rhinitis is directed to such limited molecules or directed to a wide range of self proteins, we here attempted to survey autoantigens/autoantibodies comprehensively, using proteomics. Specifically, we separated proteins extracted from peripheral blood mononuclear cells by 2-dimensional electrophoresis and then detected autoantigens by subsequent western blotting with sera from patients with allergic rhinitis. As a result, we detected multiple autoantigens, some of which were further identified by mass fingerprinting. Next, we confirmed antigenicity of one of the identified autoantigens, beta-tubulin isoform V (beta-tubV), using a recombinant protein and then measured prevalence of the anti-beta-tubV autoantibodies. As a result, 52% of the tested patients with allergic rhinitis were found to possess anti-beta-tubV autoantibodies. Our study indicates that autoimmunity is a common phenomena and beta-tubV is one of the major autoantigens in allergic rhinitis.

journal_name

Microbiol Immunol

authors

Nakamura M,Tsutsumi K,Ooka S,Sekine T,Koizuka I,Nishioka K,Kato T

doi

10.1111/j.1348-0421.2004.tb03532.x

keywords:

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

427-34

issue

5

eissn

0385-5600

issn

1348-0421

journal_volume

48

pub_type

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