Japanese patients with chronic fatigue syndrome are negative for known retrovirus infections.

abstract：:To detect neutralization-relevant antibodies against 3 types of poliovirus (PV) without using tissue cultures and live viruses, an enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibody-binding inhibition was evaluated using sera from 80 vaccinated Japanese children and 60 Pakistani poliomyelitis patie...

journal_title：Microbiology and immunology

authors： Hashido M,Horie H,Abe S,Doi Y,Hashizume S,Agboatwalla M,Isomura S,Nishio O,Hagiwara A,Inouye S

更新日期：1999-01-01 00:00:00

abstract：:In a previous study, it was found that polyoxotungstates such as undecatungstosilicate (SiW11) greatly sensitized strains of methicillin-resistant Staphylococcus aureus (MRSA) to beta-lactams. In this report, the effects of SiW11 on several MRSA strains with unique resistant mechanisms were studied. SiW11 was still ef...

journal_title：Microbiology and immunology

authors： Tajima Y

更新日期：2003-01-01 00:00:00

abstract：:The standard products of V(D)J recombination of immunoglobulin and T cell receptor genes are two kinds of DNA junction, a coding joint and a signal joint. TCR delta V-D and D-D signal joints in adult mouse thymocytes were sequenced following PCR amplification. We observed differential nucleotide insertions at the V de...

journal_title：Microbiology and immunology

authors： Kanari Y,Muto M,Yamagishi H

更新日期：2003-01-01 00:00:00

abstract：:The effect of host cell factors on infectivity of human immunodeficiency virus type 1 (HIV-1) was studied by infecting a monoblastoid cell line (U937) or a T-cell line (MOLT-4) with a highly infective single clone of HIV-1 and comparing the infectivity of the produced viruses to different cell lines. Chronically infec...

journal_title：Microbiology and immunology

authors： el-Farrash MA,Masuda T,Kuroda MJ,Harada S

更新日期：1993-01-01 00:00:00

abstract：:We previously reported the establishment of an HEp2 cell line which expresses the US3 protein kinase (PK) of herpes simplex virus type 2 (HSV-2) upon induction with IPTG. Here we report that expression, phosphorylation and ubiquitination of cytokeratin 17 (CK17) are enhanced in US3-expressing HEp2 cells. In vitro kina...

journal_title：Microbiology and immunology

authors： Murata T,Goshima F,Nishizawa Y,Daikoku T,Takakuwa H,Ohtsuka K,Yoshikawa T,Nishiyama Y

更新日期：2002-01-01 00:00:00

abstract：:An important toxin-antitoxin (TA) system hok/sok, encoded by R1 plasmid of Escherichia coli, is involved in the post segregation killing of cells that have lost the plasmid. The lethal properties of hok protein have been utilized for the environmental containment of microbes and the development of potential vaccine ca...

journal_title：Microbiology and immunology

authors： Kaur A,Muthukumarappa T,Kanta P,Banday AZ,Chidananda MK

更新日期：2020-11-01 00:00:00

abstract：:B lymphocytes express multiple TLRs that regulate their cytokine production.We investigated the effect of TLR4 and TLR9 activation on receptor activator of NF‐kB ligand (RANKL) expression by rat spleen B cells. Splenocytes or purified spleen B cells from Rowett rats were cultured with TLR4 ligand Escherichia coli LPS ...

journal_title：Microbiology and immunology

authors： Yu X,Lin J,Yu Q,Kawai T,Taubman MA,Han X

更新日期：2014-01-01 00:00:00

abstract：:The pea cyv1 gene is a yet-to-be-identified recessive resistance gene that inhibits the infection of clover yellow vein virus (ClYVV). Previous studies confirmed that the cell-to-cell movement of ClYVV is inhibited in cyv1-carrying pea plants; however, the effect of cyv1 on viral replication remains unknown. In this s...

journal_title：Microbiology and immunology

authors： Taninaka Y,Nakahara KS,Hagiwara-Komoda Y

更新日期：2020-01-01 00:00:00

abstract：:Using a new plaque cloning technique, we obtained unique Chlamydia trachomatis strains which were confirmed to be free of the 7.5-kb common plasmid and glycogen in inclusions. The in vitro susceptibility of these strains to various chemotherapeutic agents was tested by comparison with their parent strains and clinical...

journal_title：Microbiology and immunology

authors： Miyashita N,Matsumoto A,Matsushima T

更新日期：2000-01-01 00:00:00

abstract：:To evaluate DNA fingerprinting as an epidemiologic tool, pulsed-field gel electrophoresis (PFGE) was performed on isolates of Salmonella, including S. typhimurium, S. thompson, and S. enteritidis. Chromosomal DNA was digested with the restriction endonucleases Bln I and Xba I. The patterns of S. thompson and S. typhim...

journal_title：Microbiology and immunology

authors： Murase T,Okitsu T,Suzuki R,Morozumi H,Matsushima A,Nakamura A,Yamai S

更新日期：1995-01-01 00:00:00

abstract：:The US3 of HSV encodes a serine/threonine protein kinase that is highly conserved among members of the alphaherpesviruses. It is an accessory gene that is not required for viral replication in cultured cells but appears essential for viral survival in humans. Although accumulating in vitro evidence suggested that the ...

journal_title：Microbiology and immunology

authors： Mori I

更新日期：2012-06-01 00:00:00

abstract：:A mutant MT of Micrococcus lysodeikticus (luteus) IFO 3333, whose minimum growing unit is not a single cell, but a tetrad unlike the wild-type divides by binary fission of each monococcus, and then separates first into two daughter tetrads, second into four tetrads and third into eight tetrads. The three planes of eit...

journal_title：Microbiology and immunology

authors： Monodane T,Matsushima Y,Kotani S

更新日期：1978-01-01 00:00:00

abstract：:The serovar-specific main antigen (TM antigen) of Leptospira interrogans serovar canicola, which as lipopolysaccharide properties, was treated with 0.1 N sodium hydroxide. This treatment degraded the antigen into two major antigenic components, one of high and one of low molecular weight. The component with the lower ...

journal_title：Microbiology and immunology

authors： Tsuji M,Kawaoka Y,Naiki M,Yanagawa R

更新日期：1981-01-01 00:00:00

abstract：:Although the BCG vaccine can prevent tuberculosis (TB) in infants, its ability to prevent adult pulmonary TB is reportedly limited. Therefore, development of a novel effective vaccine against pulmonary TB has become an international research priority. We have previously reported that intranasal vaccination of mice wit...

journal_title：Microbiology and immunology

authors： Fukui M,Shinjo K,Umemura M,Shigeno S,Harakuni T,Arakawa T,Matsuzaki G

更新日期：2015-12-01 00:00:00

abstract：:The NfxC-type mutant of Pseudomonas aeruginosa produces the MexEF-OprN efflux pump and down-regulates expression of the quorum-sensing-dependent efflux pump MexAB-OprM and production of virulence factors in the presence of an active transcriptional regulator, MexT. Consequently, these cells are resistant to chloramphe...

journal_title：Microbiology and immunology

authors： Uwate M,Ichise YK,Shirai A,Omasa T,Nakae T,Maseda H

更新日期：2013-04-01 00:00:00

abstract：:Escherichia coli O157 strains starved in sterile deionized water (SDW) and filter-sterilized natural river water (SRW) were investigated with specific reference to their culturability in selective and non-selective media. Growth of the strains starved in both SDW and SRW were markedly suppressed with time in selective...

journal_title：Microbiology and immunology

authors： Sata S,Osawa R,Asai Y,Yamai S

更新日期：1999-01-01 00:00:00

abstract：:We have previously reported a mouse model of severe group A streptococcal infection (Microbiol. Immunol. 45: 777-786, 2001). When we injected Streptococcus pyogenes strains intramuscularly, the mice suffered from acute phase of infection for a few days but recovered from the illness and gained body weight. These mice,...

journal_title：Microbiology and immunology

authors： Iida K,Seki M,Saito M,Kawamura Y,Kajiwara H,Yoshida S

更新日期：2006-01-01 00:00:00

abstract：:Previous studies have demonstrated that the murine monoclonal antibody (MoAb) NM-01 activates the human complement classical pathway resulting in lysis of human immunodeficiency virus (HIV). The present study was performed to determine the availability of the V3-loop of gp120 relative to the complement regulatory prot...

journal_title：Microbiology and immunology

authors： Nakamura M,Okada H,Sasaki H,Yoshida K,Kamada M,Okada N,Terada M,Ohno T

更新日期：1996-01-01 00:00:00

abstract：:Although the RC-HL strain of rabies virus is avirulent in adult mice, the amino acid at position 333 of its G protein is arginine, which is thought to be necessary for virulence in adult mice upon intracerebral inoculation of the virus. This result suggests that besides arginine at position 333, some other positions o...

journal_title：Microbiology and immunology

authors： Ito H,Minamoto N,Watanabe T,Goto H,Rong LT,Sugiyama M,Kinjo T,Mannen K,Mifune K,Konobe T

更新日期：1994-01-01 00:00:00

abstract：:Rapid western blot (WB) procedure for an abnormal isoform of prion protein (PrP(Sc) ) detection in lymphoid tissues was established and has been applied to the surveillance of fallen stock. In this program, brain and palatal tonsil were examined by WB and three cases of sheep scrapie were detected. While one clinicall...

journal_title：Microbiology and immunology

authors： Shimada K,Hayashi HK,Ookubo Y,Iwamaru Y,Imamura M,Takata M,Schmerr MJ,Shinagawa M,Yokoyama T

更新日期：2005-01-01 00:00:00

abstract：:A gene for protein Arp4, an IgA receptor protein derived from Streptococcus pyogenes AP4, was expressed in Escherichia coli. The product was demonstrated to be accumulated in a periplasmic space as a polypeptide with an apparent molecular weight of 40 kDa with the deleted C-terminal membrane anchor portion of protein ...

journal_title：Microbiology and immunology

authors： Nakatani T,Higuchi A,Noguchi H

更新日期：1992-01-01 00:00:00

abstract：:Development of accurate methods for predicting progression of tuberculosis (TB) from the latent state is recognized as vitally important in controlling TB, because a majority of cases develop from latent infections. Past TB that has never been treated has a higher risk of progressing than does latent Mycobacterium tub...

journal_title：Microbiology and immunology

authors： Osada-Oka M,Tateishi Y,Hirayama Y,Ozeki Y,Niki M,Kitada S,Maekura R,Tsujimura K,Koide Y,Ohara N,Yamamoto T,Kobayashi K,Matsumoto S

更新日期：2013-01-01 00:00:00

abstract：:A protease was purified from a strain of Vibrio vulnificus isolated from the blood of a septicemic human. The vibrio was cultured in bacto peptone-yeast extract medium, and the protease was purified by a purification procedure including ultrafiltration of the culture supernatant with an Amicon YM 5 membrane, diethylam...

journal_title：Microbiology and immunology

authors： Miyoshi N,Shimizu C,Miyoshi S,Shinoda S

更新日期：1987-01-01 00:00:00

abstract：:proteins which were able to bind noncovalently with mouse factor B were found in cells that are nonsecretors of factor B such as mouse-established monocytic cells and L cells but not in peritoneal resident macrophages. These proteins were isolated from lysates of L cells and separated into four distinct proteins by pr...

journal_title：Microbiology and immunology

authors： Moriyama T,Kawamoto Y,Ichikawa H,Okamoto K,Inoue T,Kobayashi T,Miyama A

更新日期：1983-01-01 00:00:00

abstract：:The Env and Gag proteins of HIV-1 are the two major structural proteins of this retrovirus. The interactions between Env and Gag proteins and their regulation in HIV-1 are required for several steps of the replication cycle, involving not only virus assembly, specifically Env incorporation, but also entry steps after ...

journal_title：Microbiology and immunology

authors： Murakami T

更新日期：2008-05-01 00:00:00

abstract：:Measuring the amount of HIV-1 DNA in infected cells is important to estimate the size of the viral reservoir in patients. However, the clinical impact of the intracellular viral DNA level remains unclear. The present study examines the clinical significance of the HIV-1 DNA level in peripheral CD4+ T lymphocytes from ...

journal_title：Microbiology and immunology

authors： Wada K,Nagai H,Hagiwara T,Ibe S,Utsumi M,Kaneda T

更新日期：2004-01-01 00:00:00

abstract：:Oral administration of adenovirus strain K87 to BALB/c nude mice resulted in viral proliferation in the intestinal tract up to around week 6 at which point replication was suppressed. In other words, the host acquired resistance. However, this resistance was temporary and the viral infection persisted over a long peri...

journal_title：Microbiology and immunology

authors： Umehara K,Hirakawa M,Hashimoto K

更新日期：1984-01-01 00:00:00

abstract：:The possible presence of Paul-Bunnell (PB) antigen on Epstein-Barr virus (EBV)-transformed lymphocytes were investigated. Of 23 EBV genome-positive lymphoblastoid cell lines tested all but one absorbed PB type antibody from the serum of an infectious mononucleosis patient. The one EBV-negative B cell line tested did n...

journal_title：Microbiology and immunology

authors： Maeda M,Sairenji T,Hinuma Y

更新日期：1979-01-01 00:00:00

abstract：:Cross-linking of surface receptors in hematopoietic cells results in the enrichment of these receptors in the rafts along with other downstream signaling molecules. A possible explanation how signal is transduced through the plasma membrane has arisen from the concept of raft. From the study of cellular responses in t...

journal_title：Microbiology and immunology

authors： Katagiri YU,Kiyokawa N,Fujimoto J

更新日期：2001-01-01 00:00:00

abstract：:Despite the high prevalence of highly pathogenic H5N1 influenza A viruses in Indonesia, epidemiology information on seasonal human influenza is lacking. The present authors, therefore, conducted virologic surveillance in Surabaya, East Java from October 2008 to March 2010. Influenza viruses, including pandemic (H1N1) ...

journal_title：Microbiology and immunology

authors： Yamaoka M,Palilingan JF,Wibisono J,Yudhawati R,Nidom RV,Alamudi MY,Ginting TE,Makino A,Nidom CA,Shinya K,Kawaoka Y

更新日期：2011-07-01 00:00:00