Evaluation of DNA fingerprinting by PFGE as an epidemiologic tool for Salmonella infections.

Abstract:

:To evaluate DNA fingerprinting as an epidemiologic tool, pulsed-field gel electrophoresis (PFGE) was performed on isolates of Salmonella, including S. typhimurium, S. thompson, and S. enteritidis. Chromosomal DNA was digested with the restriction endonucleases Bln I and Xba I. The patterns of S. thompson and S. typhimurium isolates from various sources were different from one another. There was no correlation between the phage type and the digestion pattern of S. enteritidis isolates. Some strains belonging to one phage type were distinguished by their PFGE pattern in this study. These results suggest that the Bln I and Xba I digestion patterns of chromosomal DNA are useful for epidemiological analysis of an outbreak of Salmonella infection or food poisoning.

journal_name

Microbiol Immunol

authors

Murase T,Okitsu T,Suzuki R,Morozumi H,Matsushima A,Nakamura A,Yamai S

doi

10.1111/j.1348-0421.1995.tb03255.x

subject

Has Abstract

pub_date

1995-01-01 00:00:00

pages

673-6

issue

9

eissn

0385-5600

issn

1348-0421

journal_volume

39

pub_type

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