Evaluation of DNA fingerprinting by PFGE as an epidemiologic tool for Salmonella infections.


:To evaluate DNA fingerprinting as an epidemiologic tool, pulsed-field gel electrophoresis (PFGE) was performed on isolates of Salmonella, including S. typhimurium, S. thompson, and S. enteritidis. Chromosomal DNA was digested with the restriction endonucleases Bln I and Xba I. The patterns of S. thompson and S. typhimurium isolates from various sources were different from one another. There was no correlation between the phage type and the digestion pattern of S. enteritidis isolates. Some strains belonging to one phage type were distinguished by their PFGE pattern in this study. These results suggest that the Bln I and Xba I digestion patterns of chromosomal DNA are useful for epidemiological analysis of an outbreak of Salmonella infection or food poisoning.


Microbiol Immunol


Murase T,Okitsu T,Suzuki R,Morozumi H,Matsushima A,Nakamura A,Yamai S




Has Abstract


1995-01-01 00:00:00












  • Small ruminant lentiviral Vif proteins commonly utilize cyclophilin A, an evolutionarily and structurally conserved protein, to degrade ovine and caprine APOBEC3 proteins.

    abstract::Mammals have co-evolved with retroviruses, including lentiviruses, over a long period. Evidence supporting this contention is that viral infectivity factor (Vif) encoded by lentiviruses antagonizes the anti-viral action of cellular apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3 (APOBEC3) of the host...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Yoshikawa R,Izumi T,Nakano Y,Yamada E,Moriwaki M,Misawa N,Ren F,Kobayashi T,Koyanagi Y,Sato K

    更新日期:2016-06-01 00:00:00

  • Serological evidence of leptospiral infection in pig populations in different districts in Japan.

    abstract::Serum samples were collected from 938 pigs of 24 farms in Hokkaido, Kagoshima, and Okinawa prefectures in Japan in 2001-2005. Enzyme-linked immunosorbent assay (ELISA) was used for the detection of antibodies to LipL32 antigen which is common to Leptospira interrogans. Samples positive in ELISA were then investigated ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Naito M,Sakoda Y,Kamikawa T,Nitta Y,Hirose K,Sakashita M,Kurokawa S,Kida H

    更新日期:2007-01-01 00:00:00

  • Endogenous inhibitors of factor B.

    abstract::proteins which were able to bind noncovalently with mouse factor B were found in cells that are nonsecretors of factor B such as mouse-established monocytic cells and L cells but not in peritoneal resident macrophages. These proteins were isolated from lysates of L cells and separated into four distinct proteins by pr...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Moriyama T,Kawamoto Y,Ichikawa H,Okamoto K,Inoue T,Kobayashi T,Miyama A

    更新日期:1983-01-01 00:00:00

  • Protective effects of in vivo-expressed autotransporters against Bordetella pertussis infection.

    abstract::Bordetella pertussis causes whooping cough, a severe and prolonged respiratory disease that results inhas high morbidity and mortality rates, particularly in developing countries. The number incidence of whooping cough cases is increasing in many countries despite high vaccine coverage. Causes for the re-emergence of ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Suzuki K,Shinzawa N,Ishigaki K,Nakamura K,Abe H,Fukui-Miyazaki A,Ikuta K,Horiguchi Y

    更新日期:2017-09-01 00:00:00

  • Antiplague antibodies against Yersinia pestis fraction-I antigen in serum from rodents either experimentally infected or captured in harbor areas of Japan, 1971--1977.

    abstract::Antiplague indirect hemagglutination tests were investigated with Yersinia pestis fraction-I antigen and sera from experimentally infected rodents. The same tests with sera treated with acetone or acetone-2-mercaptoethanol were performed in parallel. Technical conditions were determined to insure the specificity and r...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Suzuki S,Hotta S

    更新日期:1979-01-01 00:00:00

  • Detection sensitivity of influenza rapid diagnostic tests.

    abstract::The sensitivity of influenza rapid diagnostic tests (IRDTs) currently available in Japan for various influenza virus strains, including human H7N9 and H5N1 isolates, were compared and it was found that all of the IRDTs examined detected these viruses; however, their detection sensitivities differed. ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Sakai-Tagawa Y,Ozawa M,Yamada S,Uchida Y,Saito T,Takahashi K,Sugaya N,Tashiro M,Kawaoka Y

    更新日期:2014-10-01 00:00:00

  • Characterization of mechanisms of fluconazole resistance in a Candida albicans isolate from a Japanese patient with chronic mucocutaneous candidiasis.

    abstract::We examined the mechanisms of fluconazole resistance in a fluconazole-resistant Candida albicans isolate from a Japanese patient with chronic mucocutaneous candidiasis. It was demonstrated that the highly resistant phenotype of this strain was associated with combined mechanisms of the energy-dependent reduced intrace...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Kamai Y,Maebashi K,Kudoh M,Makimura K,Naka W,Uchida K,Yamaguchi H

    更新日期:2004-01-01 00:00:00

  • Cluster analysis of human and animal pathogenic Microsporum species and their teleomorphic states, Arthroderma species, based on the DNA sequences of nuclear ribosomal internal transcribed spacer 1.

    abstract::We performed a cluster analysis of human and animal pathogenic Microsporum species and their teleomorphic states, Arthroderma species, including A. otae-related species (M. canis, M. audouinii, M. distortum, M. equinum, M. langeronii, and M. ferrugineum) and M. gypseum complex (A. fulvum, A. gypseum, and A. incurvatum...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Makimura K,Tamura Y,Murakami A,Kano R,Nakamura Y,Hasegawa A,Uchida K,Yamaguchi H

    更新日期:2001-01-01 00:00:00

  • Properties of Hg- and Cd-spores of Bacillus megaterium QM B1551.

    abstract::Hg- and Cd-spores of Bacillus megaterium QM B1551 were produced in Schaeffer's medium containing mercuric chloride and cadmium chloride respectively. Metals were added to the medium at 9 hr of incubation (Stage V) to give a final concentration of 50 microM. It was found by electron microscopic and biochemical studies ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Imagawa M,Ichikawa T,Kondo M

    更新日期:1982-01-01 00:00:00

  • Pathogenicity of glycoprotein C-deficient herpes simplex virus 1 strain TN-1 which encodes truncated glycoprotein C.

    abstract::A clinical isolate of herpes simplex virus 1 (TN-1) from a stromal keratitis patient was found to be defective in the glycoprotein C (gC) gene (UL44), thus resulting in the production of truncated gC upon infection. To study the pathogenetic role of truncated gC, we prepared a recombinant LTN-8 derived from TN-1 with ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Minagawa H,Liu Y,Yoshida T,Hidaka Y,Toh Y,Mori R

    更新日期:1997-01-01 00:00:00

  • Class-specific antibody response in acyclovir-treated and adenine arabinoside-treated patients with primary genital herpes simplex virus infection.

    abstract::Herpes simplex virus (HSV) class-specific antibody responses after primary genital herpes infection were evaluated with an enzyme-linked immunosorbent assay (ELISA) in 16 patients treated with acyclovir (ACV), given orally, and 17 patients treated with adenine arabinoside (Ara-A), given topically. ACV significantly su...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Kawana T,Hashido M,Koizumi Y

    更新日期:1995-01-01 00:00:00

  • Macrophage migration inhibitory factor (MIF) produced by a human T cell hybridoma clone.

    abstract::A human T cell hybridoma clone, F5, producing high levels of macrophage migration inhibitory factor (MIF) was established by the emetine-actinomycin D selection method. This clone produced two species of MIF which were separated on a Phenyl Sepharose column. We purified MIF-2 (the more hydrophobic species of the two) ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Hirose S,Ooki S,Higuchi M,Osawa T

    更新日期:1991-01-01 00:00:00

  • The role of p38 mitogen-activated protein kinase in regulating interleukin-10 gene expression in Burkitt's lymphoma cell lines.

    abstract::In malignant B lymphoma cells interleukin-10 (IL-10) expression is frequently upregulated. This effect is thought to support to the malignant transformation of these cells and to be a potential target for pharmacotherapy. To define better the mechanism for upregulation of the IL-10 gene, we tested the association betw...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Horie K,Ohashi M,Satoh Y,Sairenji T

    更新日期:2007-01-01 00:00:00

  • Multicenter evaluation of prototype real-time PCR assays for Epstein-Barr virus and cytomegalovirus DNA in whole blood samples from transplant recipients.

    abstract::Quantitative PCR is becoming widespread for diagnosing and monitoring post-transplantation diseases associated with EBV and CMV. These assays need to be standardized to manage patients in different facilities. Five independent laboratories in Japan compared home-brew assays and a prototype assay system to establish a ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章,多中心研究


    authors: Ito Y,Takakura S,Ichiyama S,Ueda M,Ando Y,Matsuda K,Hidaka E,Nakatani K,Nishioka J,Nobori T,Kajiyama N,Kimura H

    更新日期:2010-09-01 00:00:00

  • Differentiation between genera Rhodococcus and Nocardia by susceptibility testing to kanamycin and some other antituberculosis agents.

    abstract::The test for susceptibility to kanamycin is useful for differentiating between the genera Rhodococcus and Nocardia. All rhodococci except R. equi, R. erythropolis, and R. aurantiacus are susceptible to kanamycin, whereas all nocardiae except N. otitidis-caviarum are resistant to kanamycin. Tests for susceptibility to ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Tsukamura M

    更新日期:1988-01-01 00:00:00

  • The matrix gene expression of subacute sclerosing panencephalitis (SSPE) virus (Osaka-1 strain): a comparison of two sibling viruses isolated from different lobes of an SSPE brain.

    abstract::The Fr/V and Oc/V sibling viruses of the Osaka-1 strain of the subacute sclerosing panencephalitis (SSPE) virus were defective in cell-free virus production. By radioimmunoprecipitation assay, the matrix (M) protein was not detected in cells persistently infected with the Osaka-1 strain. This undetectable expression w...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Ayata M,Hayashi K,Seto T,Murata R,Ogura H

    更新日期:1998-01-01 00:00:00

  • Consensus sequence on the genes encoding the major outer surface proteins (OspA and OspB) of Borrelia garinii isolate.

    abstract::Japanese Lyme borrelias classified as ribotype IV is predominant among isolates derived from clinical specimens, reservoir rodents and Ixodes persulcatus ticks, and has been characterized as Borrelia garinii. These B. garinii isolates have antigenic and genetic features apparently different from North American, Europe...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Wang J,Masuzawa T,Komikado T,Yanagihara Y

    更新日期:1997-01-01 00:00:00

  • A latex agglutination test for the detection of Mycoplasma pneumoniae in respiratory exudates: a comparative study with a commercially available DNA-probe test.

    abstract::We prepared polyclonal antibody specific to Mycoplasma pneumoniae. Using this antibody, we developed a latex agglutination test (LAT) for detecting the organism in respiratory exudates as rapid diagnosis of M. pneumoniae infection. Further, LAT was compared with DNA-probe test (DP) which was the only commercially avai...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Masayoshi T,Hirai Y,Kanemasa Y

    更新日期:1992-01-01 00:00:00

  • Formation of a hexagonal lattice structure by an R-form lipopolysaccharide of Klebsiella: comparative study of preparations extracted by the phenol-water and the phenol-chloroform-petroleum ether methods.

    abstract::An R-form lipopolysaccharide (LPS) extracted from Klebsiella strain LEN-111 (O3-:K1-) by the phenol-chloroform-petroleum ether method was compared with that extracted by the phenol-water method in the ability to form a hexagonal assembly. The LPS which was extracted by the phenol-water method and dialyzed against tap ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Kato N,Ohta M,Kido N,Ito H,Naito S,Kuno T

    更新日期:1985-01-01 00:00:00

  • Deletion polymorphisms in the promoter region of Fcgamma receptor IIB is not associated with antigen-specific IgG2a and IgG2b antibody responses in NC/Nga mice.

    abstract::Fcgamma receptor (R) IIB, a low-affinity FcR for IgG, inhibits B cell Ag R (BCR)-mediated activation when these two receptors are cross-linked by Ag and IgG-containing immune complexs (ICs). We found deletion polymorphisms in the promoter region of fcgr2b in NC/Nga mice, a model for human atopic dermatitis. NC/Nga mic...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Sakai T,Kogiso M,Mitsuya K,Komatsu T,Yamamoto S

    更新日期:2006-01-01 00:00:00

  • Antibacterial activity of cefpodoxime against Branhamella catarrhalis.

    abstract::The antibacterial activity of cefpodoxime against Branhamella catarrhalis was studied. All of the 65 clinical isolates tested were inhibited at and below 1.56 micrograms/ml, both at 10(7) and at 10(5) CFUs. The following was further studied on B. catarrhalis N-5 which showed average susceptibility to each drug examine...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Takenouchi T,Nishino T

    更新日期:1991-01-01 00:00:00

  • MASTDISCS combi Carba plus, a simple method for discriminating carbapenemase-producing Enterobacteriaceae, including OXA-48-type producers.

    abstract::Accurate and rapid detection of carbapenemases and identification of their types in Enterobacteriaceae are both still major challenges for clinical laboratories in attempting to prevent the intrusion and transmission of carbapenemase-producing Enterobacteriaceae. This study aimed to evaluate the performance of the MAS...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Ohsaki Y,Kubo R,Hobson J,Davies M,Osaka S,Hayashi W,Taniguchi Y,Koide S,Nagano Y,Nagano N

    更新日期:2018-01-01 00:00:00

  • Electron microscopic study of hemadsorption on vaccinia virus infected cells.

    abstract::Hemadsorption (HAD) induced in HEp-2 cells infected with vaccinia virus was observed. In ultrathin sections, binding of 36 red blood cells (RBCs) was examined in detail and 3 types of HAD were observed: (1) direct and close binding of RBCs to infected HEp-2 cells (cyto-HAD) was observed in cross sections of 27 RBCs (2...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Saburi Y,Okuda K,Takahashi T,Tadokoro I

    更新日期:1977-10-20 00:00:00

  • Activation of Toll‐like receptor 9 inhibits lipopolysaccharide‐induced receptor activator of nuclear factor kappa‐ B ligand expression in rat B lymphocytes.

    abstract::B lymphocytes express multiple TLRs that regulate their cytokine production.We investigated the effect of TLR4 and TLR9 activation on receptor activator of NF‐kB ligand (RANKL) expression by rat spleen B cells. Splenocytes or purified spleen B cells from Rowett rats were cultured with TLR4 ligand Escherichia coli LPS ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Yu X,Lin J,Yu Q,Kawai T,Taubman MA,Han X

    更新日期:2014-01-01 00:00:00

  • Genetic control of immune response to a synthetic fimbrial antigen of Actinobacillus actinomycetemcomitans.

    abstract::The incidence of infection by Actinobacillus actinomycetemcomitans, one of the important pathogens in human periodontal diseases, has been reported to be associated with racial background and genetic factors. We attempted to determine the genetic regulation of immune responses to A. actinomycetemcomitans fimbriae, an ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Honma K,Ishii N,Kato T,Ishihara K,Okuda K,Okuda K

    更新日期:1997-01-01 00:00:00

  • Molecular determination of infection source of a sporadic Legionella pneumonia case associated with a hot spring bath.

    abstract::To determine the infection source of a sporadic Legionella pneumonia case associated with a hot spring bath, we used five molecular methods, including repetitive element polymerase chain reaction (rep-PCR), arbitrarily primed PCR (AP-PCR), ribotyping, restriction endonuclease analysis (REA), and macrorestriction endon...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Miyamoto H,Jitsurong S,Shiota R,Maruta K,Yoshida S,Yabuuchi E

    更新日期:1997-01-01 00:00:00

  • Human T-lymphoblastoid cell lines with high and low abilities to produce interferon-gamma constitutively and their susceptibilities to interferon.

    abstract::A human T-lymphoblastoid cell line, TCL-Fuj, produces large amounts of interferon (IFN)-gamma constitutively. A variant cell line, 2M, was derived from it. Both cell lines express similar surface antigen markers, but differ in surface morphology. Compared with the parent TCL-Fuj cell line, 2M produced less IFN-gamma c...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Matsuyama M,Sugamura K,Hinuma Y

    更新日期:1987-01-01 00:00:00

  • Induction of acute arthritis in mice by peptidoglycan derived from gram-positive bacteria and its possible role in cytokine production.

    abstract::The activities of a water-soluble peptidoglycan fragment derived from Staphylococcus epidermidis (SEPS) were examined as to their role in proliferation of spleen mononuclear cells (SMNC) from various strains of mice, the production of cytokines in vitro, and the induction of an inflammatory reaction in vivo. The proli...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Onta T,Sashida M,Fujii N,Sugawara S,Rikiishi H,Kumagai K

    更新日期:1993-01-01 00:00:00

  • Genus Enhydrobacter Staley et al. 1987 should be recognized as a member of the family Rhodospirillaceae within the class Alphaproteobacteria.

    abstract::The genus Enhydrobacter, first reported as a member of the family Vibrionaceae, has been placed in the family Moraxellaceae, but as a genus incertae sedis in Bergey's Manual of Systematic Bacteriology 2nd edition. During our taxonomic investigation of Enhydrobacter-like organisms, we observed that the 16S rRNA sequenc...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Kawamura Y,Fujiwara N,Naka T,Mitani A,Kubota H,Tomida J,Morita Y,Hitomi J

    更新日期:2012-01-01 00:00:00

  • Comparative characterization of inducible and virulent Vibrio parahaemolyticus bacteriophages having unique head projections.

    abstract::Phage TP1, induced from Vibrio parahaemolyticus K-20 pilot strain by mitomycin C, exhibited a unique hexagonal head with knob-like projections which covered the whole capsid and a noncontractile tail. The appearance of this phage was very similar to those of phages VP3 and VP6, isolated from seawater. The host range o...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Koga T,Kawata T

    更新日期:1991-01-01 00:00:00