Evaluation of DNA fingerprinting by PFGE as an epidemiologic tool for Salmonella infections.


:To evaluate DNA fingerprinting as an epidemiologic tool, pulsed-field gel electrophoresis (PFGE) was performed on isolates of Salmonella, including S. typhimurium, S. thompson, and S. enteritidis. Chromosomal DNA was digested with the restriction endonucleases Bln I and Xba I. The patterns of S. thompson and S. typhimurium isolates from various sources were different from one another. There was no correlation between the phage type and the digestion pattern of S. enteritidis isolates. Some strains belonging to one phage type were distinguished by their PFGE pattern in this study. These results suggest that the Bln I and Xba I digestion patterns of chromosomal DNA are useful for epidemiological analysis of an outbreak of Salmonella infection or food poisoning.


Microbiol Immunol


Murase T,Okitsu T,Suzuki R,Morozumi H,Matsushima A,Nakamura A,Yamai S




Has Abstract


1995-01-01 00:00:00












  • Epidemiological analysis of serum anti-Pseudomonas aeruginosa PcrV titers in adults.

    abstract::Of the various virulence mechanisms of the opportunistic pathogen Pseudomonas aeruginosa, the type III secretion system (TTSS) has been characterized as a major factor associated with acute lung injury, bacteremia and mortality. In addition, PcrV, a component protein of the TTSS, has been characterized as a protective...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Yasumoto H,Katoh H,Kinoshita M,Shimizu M,Hamaoka S,Akiyama K,Naito Y,Sawa T

    更新日期:2016-02-01 00:00:00

  • Metastasis-promoting activity of a novel molecule, Ag 243-5, derived from mycoplasma, and the complete nucleotide sequence.

    abstract::We found that mycoplasma-infected cells have a higher ability to metastasize in vivo than non-mycoplasma-infected cells. To investigate this phenomenon, we obtained a monoclonal antibody, MAb 243-5, by immunization with Mycoplasma arginini-infected RPMI 4788 cells. This MAb recognized a mycoplasmal protein with an MW ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Ushio S,Iwaki K,Taniai M,Ohta T,Fukuda S,Sugimura K,Kurimoto M

    更新日期:1995-01-01 00:00:00

  • Engineering large viral DNA genomes using the CRISPR-Cas9 system.

    abstract::Manipulation of viral genomes is essential for studying viral gene function and utilizing viruses for therapy. Several techniques for viral genome engineering have been developed. Homologous recombination in virus-infected cells has traditionally been used to edit viral genomes; however, the frequency of the expected ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Suenaga T,Kohyama M,Hirayasu K,Arase H

    更新日期:2014-09-01 00:00:00

  • Spontaneous production of a C-type RNA virus in a cell line derived from rat glioma.

    abstract::The spontaneous production of a rat C-type RNA virus (ACV) in a cultured cell line (AC cells) established from a chemically induced rat glioma was studied. The characteristics of ACV were: morphology typical of C-type RNA virus; buoyant density of 1.15 g/ml in a sucrose density gradient; RNA directed DNA polymerase ac...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Igarashi K,Sasada R,Niiyama Y,Kozai Y,Sugino Y

    更新日期:1979-01-01 00:00:00

  • Class-specific antibody response in acyclovir-treated and adenine arabinoside-treated patients with primary genital herpes simplex virus infection.

    abstract::Herpes simplex virus (HSV) class-specific antibody responses after primary genital herpes infection were evaluated with an enzyme-linked immunosorbent assay (ELISA) in 16 patients treated with acyclovir (ACV), given orally, and 17 patients treated with adenine arabinoside (Ara-A), given topically. ACV significantly su...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Kawana T,Hashido M,Koizumi Y

    更新日期:1995-01-01 00:00:00

  • Antibacterial activity of cefpodoxime against Branhamella catarrhalis.

    abstract::The antibacterial activity of cefpodoxime against Branhamella catarrhalis was studied. All of the 65 clinical isolates tested were inhibited at and below 1.56 micrograms/ml, both at 10(7) and at 10(5) CFUs. The following was further studied on B. catarrhalis N-5 which showed average susceptibility to each drug examine...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Takenouchi T,Nishino T

    更新日期:1991-01-01 00:00:00

  • Isolation and characterization of temperature-sensitive mutants of Sendai virus.

    abstract::Sixteen temperature-sensitive mutants of Sendai virus were isolated from mutagenized stocks (10 mutants, designated numerically) and persistently infected cultures (6 mutants, designated alphabetically). Based on complementation tests, virion-associated activities, thermal inactivation, and viral RNA and hemadsorbing ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Adachi A,Kanda T,Shibuta H

    更新日期:1980-01-01 00:00:00

  • Effect of Yersinia enterocolitica ST on cyclic guanosine 3',5'-monophosphate levels in mouse intestines and cultured cells.

    abstract::We determined the effect of heat-stable enterotoxin produced by Yersinia enterocolitica (Y. enterocolitica ST) on cyclic nucleotide levels in the intestines of 6-day-old mice and in cultured cell line cells. The concentration of cyclic guanosine 3',5'-monophosphate (cyclic GMP) in homogenates of the intestines increas...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Inoue T,Okamoto K,Moriyama T,Takahashi T,Shimizu K,Miyama A

    更新日期:1983-01-01 00:00:00

  • Protective effect of recombinant human interleukin-2 against lethal infection caused by Klebsiella pneumoniae.

    abstract::The effects of recombinant human interleukin-2 (rIL-2) administered prophylactically on the death of CBA/J mice challenged with Klebsiella pneumoniae 27 intraperitoneally were examined. rIL-2 administered subcutaneously at 20 micrograms per mouse for 7 days enhanced survival after a lethal challenge. The injection of ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Iizawa Y,Nakao M,Kondo M,Yamazaki T

    更新日期:1990-01-01 00:00:00

  • Electron microscopic study of hemadsorption on vaccinia virus infected cells.

    abstract::Hemadsorption (HAD) induced in HEp-2 cells infected with vaccinia virus was observed. In ultrathin sections, binding of 36 red blood cells (RBCs) was examined in detail and 3 types of HAD were observed: (1) direct and close binding of RBCs to infected HEp-2 cells (cyto-HAD) was observed in cross sections of 27 RBCs (2...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Saburi Y,Okuda K,Takahashi T,Tadokoro I

    更新日期:1977-10-20 00:00:00

  • Herpes simplex virus US3 protein kinase regulates host responses and determines neurovirulence.

    abstract::The US3 of HSV encodes a serine/threonine protein kinase that is highly conserved among members of the alphaherpesviruses. It is an accessory gene that is not required for viral replication in cultured cells but appears essential for viral survival in humans. Although accumulating in vitro evidence suggested that the ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章,评审


    authors: Mori I

    更新日期:2012-06-01 00:00:00

  • Increased phosphodiesters in lipopolysaccharide prepared from the polymyxin B-resistant isolate of Klebsiella pneumoniae.

    abstract::A polymyxin B (PXB)-resistant mutant of Klebsiella pneumoniae O3 was isolated. Lipopolysaccharide (LPS) extracted from the PXB-resistant isolate bound little PXB, although LPS from the parental strain did. The 31P nuclear magnetic resonance (NMR) spectrum of PXB-resistant type LPS showed that it contained much less of...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Yokochi T,Inoue Y,Jiang GZ,Kato Y,Sugiyama T,Kawai M,Fukada M,Takahashi K

    更新日期:1994-01-01 00:00:00

  • Genetic control of immune response to a synthetic fimbrial antigen of Actinobacillus actinomycetemcomitans.

    abstract::The incidence of infection by Actinobacillus actinomycetemcomitans, one of the important pathogens in human periodontal diseases, has been reported to be associated with racial background and genetic factors. We attempted to determine the genetic regulation of immune responses to A. actinomycetemcomitans fimbriae, an ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Honma K,Ishii N,Kato T,Ishihara K,Okuda K,Okuda K

    更新日期:1997-01-01 00:00:00

  • Inhibition of HIV-1 infection by an intramolecular antisense peptide to T20 in gp160.

    abstract::Antisense amino acids are amino acids which can be translated from the corresponding anti-codons of a sense amino acid. Antisense peptides encoded by the noncoding DNA strand have a tendency to interact with each other. We have demonstrated that antisense peptide sequences are present intramolecularly, and these may c...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Imai M,Okada N,Okada H

    更新日期:2000-01-01 00:00:00

  • Protective capacity of L-form Salmonella typhimurium against murine typhoid in C3H/HeJ mice.

    abstract::L forms of Salmonella typhimurium LT2 conferred strong protection to a lethal challenge with its parental bacterium on innately hypersusceptible C3H/HeJ mice, and its minimal protective dose was approximately 150 L-forming units. Although L-form S. typhimurium was avirulent for C3H/HeJ mice, it multiplied slowly in bo...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Nishikawa F,Kita E,Yamada H,Nakano A,Kashiba S

    更新日期:1994-01-01 00:00:00

  • Prevalence, molecular characteristics and risk factors for cryptosporidiosis among Iranian immunocompromised patients.

    abstract::Cryptosporidium spp. is a major cause of diarrhea in developing countries, mainly affecting people with compromised immune systems in general and HIV-infected individuals with low CD4 + T-cell counts in particular. This infection is self-limiting in healthy persons; however, it can be severe, progressive and persisten...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Izadi M,Jonaidi-Jafari N,Saburi A,Eyni H,Rezaiemanesh MR,Ranjbar R

    更新日期:2012-12-01 00:00:00

  • Oral immunization of mice with Saccharomyces cerevisiae expressing a neutralizing epitope of ApxIIA exotoxin from Actinobacillus pleuropneumoniae induces systemic and mucosal immune responses.

    abstract::An oral delivery system based on ApxIIA#5-expressed on Saccharomyces cerevisiae was studied for its potential to induce immune responses in mice. Murine bone marrow-derived dendritic cells (DCs) stimulated in vitro with ApxIIA#5-expressed on S. cerevisiae upregulated the expression of maturation and activation markers...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Shin MK,Lee WJ,Jung MH,Cha SB,Shin SW,Yoo A,Kim DH,Yoo HS

    更新日期:2013-06-01 00:00:00

  • Novel functions of herbal medicines in dendritic cells: role of Amomi Semen in tumor immunity.

    abstract::Dendritic cells (DCs) have a major role in regulating immune responses, including tumor immunity and peripheral tolerance. In the present study, we identified novel functions of herbal medicines in DCs by screening 99 herbal medicines, most of which are among the 210 Chinese medicines approved by the Ministry of Healt...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Fukui H,Mitsui S,Harima N,Nose M,Tsujimura K,Mizukami H,Morita A

    更新日期:2007-01-01 00:00:00

  • The fibronectin-binding protein homologue Fbp62 of Streptococcus anginosus is a potent virulence factor.

    abstract::Streptococcus anginosus appears to be able to adhere to cultured epithelial cells or fibronectin and this may be associated with bacterial pathogenicity. In the present study, the molecular characteristics and virulence of the fibronectin-binding protein (FBP), Fbp62, of S. anginosus were investigated in animal models...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Kodama Y,Ishikawa T,Shimoyama Y,Sasaki D,Kimura S,Sasaki M

    更新日期:2018-10-01 00:00:00

  • In vitro models for analysis of the hepatitis C virus life cycle.

    abstract::Chronic hepatitis C virus (HCV) infection affects approximately 170 million people worldwide. HCV infection is a major global health problem as it can be complicated with liver cirrhosis and hepatocellular carcinoma. So far, there is no vaccine available and the non-specific, interferon (IFN)-based treatments now in u...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章,评审


    authors: Aly HH,Shimotohno K,Hijikata M,Seya T

    更新日期:2012-01-01 00:00:00

  • Enhancement of antigen-presenting function of dendritic cells with culture supernatants of mouse peritoneal macrophages stimulated with certain particulate substances.

    abstract::The production from murine resident peritoneal macrophages (M phi) of a soluble factor, which was capable of enhancing the antigen-presenting (AP) function of dendritic cells (DC), was examined. The supernatants of peritoneal M phi (M phi sup) were prepared by culturing peritoneal M phi with particles, i.e., zymosan A...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Miyazaki H,Ito A,Osawa T

    更新日期:1988-01-01 00:00:00

  • Phosphatidylinositol 4-kinase III beta is the target of oxoglaucine and pachypodol (Ro 09-0179) for their anti-poliovirus activities, and is located at upstream of the target step of brefeldin A.

    abstract::In recent years, phosphatidylinositol 4-kinase III beta (PI4KB) has emerged as a conserved target of anti-picornavirus compounds. In the present study, PI4KB was identified as the direct target of the plant-derived anti-picornavirus compounds, oxoglaucine and pachypodol (also known as Ro 09-0179). PI4KB was also ident...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Arita M,Philipov S,Galabov AS

    更新日期:2015-06-01 00:00:00

  • Characterization of an outer membrane protein gene, pgmA, and its gene product from Porphyromonas gingivalis.

    abstract::A gene upstream from fimA, the gene encoding fimbrilin, on the chromosome of Porphyromonas gingivalis was sequenced and shown to be the gene encoding an outer membrane protein in this organism based on homology and biochemical analyses. Therefore, the gene (formerly ORF5) was designated pgmA, the P. gingivalis outer m...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Hongo H,Osano E,Ozeki M,Onoe T,Watanabe K,Honda O,Tani H,Nakamura H,Yoshimura F

    更新日期:1999-01-01 00:00:00

  • The Bordetella type III secretion system: its application to vaccine development.

    abstract::B. pertussis is a causative agent of whooping cough (pertussis) in humans. Despite wide-scale vaccination in many countries, there is serious concern about pertussis as a re-emerging disease. Re-emergence of pertussis may be explained by several factors: the short duration of protection by the currently available acel...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章,评审


    authors: Abe A,Nagamatsu K,Watanabe M

    更新日期:2008-02-01 00:00:00

  • Severe invasive streptococcal infection by Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis.

    abstract::Streptococcus pyogenes, a group A Streptococcus (GAS), has been recognized as the causative pathogen in patients with severe invasive streptococcal infection with or without necrotizing fasciitis. In recent epidemiological studies, Streptococcus dysgalactiae subsp. equisimilis (SDSE) has been isolated from severe inva...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章,评审


    authors: Watanabe S,Takemoto N,Ogura K,Miyoshi-Akiyama T

    更新日期:2016-01-01 00:00:00

  • Stenotrophomonas maltophilia interaction with human epithelial respiratory cells in vitro.

    abstract::Bacteria of Stenotrophomonas maltophilia have been isolated with increasing frequency from the airways of cystic fibrosis (CF) patients, usually following P. aeruginosa infections, but their adherence to human epithelial respiratory cells has never been investigated. In this study, various S. maltophilia strains were ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: De Vidipó LA,De Marques EA,Puchelle E,Plotkowski MC

    更新日期:2001-01-01 00:00:00

  • Quantification of the CD55 and CD59, membrane inhibitors of complement on HIV-1 particles as a function of complement-mediated virolysis.

    abstract::Previous studies have demonstrated that the murine monoclonal antibody (MoAb) NM-01 activates the human complement classical pathway resulting in lysis of human immunodeficiency virus (HIV). The present study was performed to determine the availability of the V3-loop of gp120 relative to the complement regulatory prot...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Nakamura M,Okada H,Sasaki H,Yoshida K,Kamada M,Okada N,Terada M,Ohno T

    更新日期:1996-01-01 00:00:00

  • Genetic identification of Staphylococcus aureus by polymerase chain reaction using single-base-pair mismatch in 16S ribosomal RNA gene.

    abstract::Staphylococcus aureus is the most predominant and important pathogen in clinical microbiology. A DNA amplification assay using the polymerase chain reaction (PCR) was designed to identify S. aureus through a single-base-pair mismatch in the sequences of staphylococcal 16S ribosomal RNA (16S rRNA) genes. It was able to...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Saruta K,Hoshina S,Machida K

    更新日期:1995-01-01 00:00:00

  • The role of p38 mitogen-activated protein kinase in regulating interleukin-10 gene expression in Burkitt's lymphoma cell lines.

    abstract::In malignant B lymphoma cells interleukin-10 (IL-10) expression is frequently upregulated. This effect is thought to support to the malignant transformation of these cells and to be a potential target for pharmacotherapy. To define better the mechanism for upregulation of the IL-10 gene, we tested the association betw...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Horie K,Ohashi M,Satoh Y,Sairenji T

    更新日期:2007-01-01 00:00:00

  • Immunogenic dialyzable factor derived from a ribosomal fraction of Salmonella typhimurium III. Analysis of resistance induced by dialyzable factors.

    abstract::Dialyzable factors (DF) were prepared from ribosomal fractions of several organisms including rough mutants of Salmonella typhimurium LT2, salmonella species of different serogroups, other enteric bacteria and gram-positive organisms, and tested for their immunogenicity against S. typhimurium infection in mice. All of...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章


    authors: Kita E,Yasui K,Yasui K,Matsuda Y,Matsuda K,Kashiba S

    更新日期:1984-01-01 00:00:00