Evaluation of an enzyme-linked immunosorbent assay based on binding inhibition for type-specific quantification of poliovirus neutralization-relevant antibodies.

abstract：:The antigenic formula and chemical structure of capsular polysaccharide (CPS) of Cryptococcus albidus var. albidus (C. albidus) were studied in relation to those of C. neoformans var. neoformans serotype A (C. neoformans A). The results of slide agglutination tests with factor sera and reciprocal adsorption experiment...

journal_title：Microbiology and immunology

authors： Ikeda R,Matsuyama H,Nishikawa A,Shinoda T,Fukazawa Y

更新日期：1991-01-01 00:00:00

abstract：:A sensitive, quantitative, short-time, and reproducible focus assay for Japanese encephalitis (JE) virus is described. After 2 or 3 days of incubation, the infected cells were treated with anti-JE virus serum and complement, and subsequently stained with trypan blue; then clear foci were produced. This method made it ...

journal_title：Microbiology and immunology

authors： Kimura-Kuroda J,Yasui K

更新日期：1985-01-01 00:00:00

abstract：:The effect of fatty acids on Mycobacterium smegmatis was examined in vitro at pH 5.0 to 7.0 to determine the role of fatty acids in the intracellular killing of mycobacteria. Unsaturated fatty acids showed strong bactericidal activity in low concentrations (0.005 to 0.02 mM), whereas saturated fatty acids, except for ...

journal_title：Microbiology and immunology

authors： Kanetsuna F

更新日期：1985-01-01 00:00:00

abstract：:Samples of feces and vomit collected from patients during 13 non-bacterial gastroenteritis outbreaks which occurred in Hokkaido between 1995 and 1998 were examined by electron microscopy (EM) and reverse-transcription polymerase chain reaction (RT-PCR) for evidence of infection with human caliciviruses (HuCVs). In 6 f...

journal_title：Microbiology and immunology

authors： Ohyama T,Yoshizumi S,Sawada H,Uchiyama Y,Katoh Y,Hamaoka N,Utagawa E

更新日期：1999-01-01 00:00:00

abstract：:The inhibitory effects of an extract of the blackcurrant (Ribes nigrum L.) against pathogens associated with oral, nasopharyngeal and upper respiratory infectious diseases; namely respiratory syncytial virus (RSV), influenza virus A and B (IFV-A and IFV-B), adenovirus (AdV), herpes simplex virus type 1, Haemophilus in...

journal_title：Microbiology and immunology

authors： Ikuta K,Hashimoto K,Kaneko H,Mori S,Ohashi K,Suzutani T

更新日期：2012-12-01 00:00:00

abstract：:In Streptococcus pneumoniae, heat shock induces the synthesis of 65-, 73-, and 84-kDa proteins, and ethanol shock induces a 104-kDa protein. In this study, the 65-, 84-, and 104-kDa proteins were identified as members of the GroEL, ClpL and alcohol dehydrogenase families, respectively, and the general properties of th...

journal_title：Microbiology and immunology

authors： Choi IH,Shim JH,Kim SW,Kim SN,Pyo SN,Rhee DK

更新日期：1999-01-01 00:00:00

abstract：:We found that a sub-lethal concentration of hydrogen peroxide (HPOx) enhanced the growth of Helicobacter pylori in Brucella broth supplemented with 10% fetal bovine serum (BB/FBS). The enhancement was evident at 0.1 mM HPOx and reached a maximun at 3.5 mM. The growth stimulation was dependent on the basal media used; ...

journal_title：Microbiology and immunology

authors： Murano A,Miyake M,Kato J,Tanzawa H,Takeo K,Noda M

更新日期：1999-01-01 00:00:00

abstract：:The sensitivity of influenza rapid diagnostic tests (IRDTs) currently available in Japan for various influenza virus strains, including human H7N9 and H5N1 isolates, were compared and it was found that all of the IRDTs examined detected these viruses; however, their detection sensitivities differed. ...

journal_title：Microbiology and immunology

authors： Sakai-Tagawa Y,Ozawa M,Yamada S,Uchida Y,Saito T,Takahashi K,Sugaya N,Tashiro M,Kawaoka Y

更新日期：2014-10-01 00:00:00

abstract：:Cattle enterotoxemia caused by Clostridium perfringens toxins is a noncontagious, sporadic, and fatal disease characterized by sudden death. Strategies for controlling and preventing cattle enterotoxemia are based on systematic vaccination of herds with toxoids. Because the process of producing conventional clostridia...

journal_title：Microbiology and immunology

authors： Jiang Z,De Y,Chang J,Wang F,Yu L

更新日期：2014-11-01 00:00:00

abstract：:Staphylococcal exfoliative toxin A (ETA) had a splitting effect at the granular layer of skin in humans and neonatal mice, but not in rabbits, guinea pigs, golden hamsters, or rats. Besides its splitting effect, ETA could stimulate productions of neutralizing antibody to ETA in rabbits, rats and B10D2 mice, but not in...

journal_title：Microbiology and immunology

authors： Machida K,Sakurai S,Kondo I,Ikawa S

更新日期：1988-01-01 00:00:00

abstract：:β-1,3-glucan is a major component of fungal cell walls with various biological activities, including effects on the production of inflammatory mediators in vivo and in vitro. However, few reports have examined its influence on the fungal cell itself. In this study, the influences of β-1,3-glucan on the growth and cell...

journal_title：Microbiology and immunology

authors： Ishibashi K,Kurone Y,Motoi M,Miura NN,Adachi Y,Shirasu Y,Ohno N

更新日期：2010-11-01 00:00:00

abstract：:Unmethylated CpG-ODN are known to enhance Th1-type immune response. However, optimal sequences of CpG-ODN for activating Th1-type immune cells vary among species. It is necessary to identify the effective CpG-ODN sequences in each species. In the present study, in order to identify the sequences of CpG-ODN that produc...

journal_title：Microbiology and immunology

authors： Satoh R,Kotake M,Takano T,Motokawa K,Gemma T,Watanabe R,Arai S,Hohdatsu T

更新日期：2011-03-01 00:00:00

abstract：:Site-specific recombinase is widely applied for the regulation of gene expression because its regulatory action is strict and efficient. However, each system can mediate regulation of only one gene at a time. Here, we demonstrate efficient "sequential" gene regulation using Cre-and FLP-expressing recombinant adenoviru...

journal_title：Microbiology and immunology

authors： Kondo S,Takahashi Y,Shiozawa S,Ichise H,Yoshida N,Kanegae Y,Saito I

更新日期：2006-01-01 00:00:00

abstract：:Of the various virulence mechanisms of the opportunistic pathogen Pseudomonas aeruginosa, the type III secretion system (TTSS) has been characterized as a major factor associated with acute lung injury, bacteremia and mortality. In addition, PcrV, a component protein of the TTSS, has been characterized as a protective...

journal_title：Microbiology and immunology

authors： Yasumoto H,Katoh H,Kinoshita M,Shimizu M,Hamaoka S,Akiyama K,Naito Y,Sawa T

更新日期：2016-02-01 00:00:00

abstract：:The 16S rRNA gene sequences were determined for type strains of 21 Bifidobacterium species. A phylogenetic tree was constructed using the determined sequences and sequences from DNA databases, which contain the sequences of 11 type strains of Bifidobacterium species and 11 strains of related genera. All species of the...

journal_title：Microbiology and immunology

authors： Miyake T,Watanabe K,Watanabe T,Oyaizu H

更新日期：1998-01-01 00:00:00

abstract：:To clarify the epidemiology of viral acute respiratory infections (ARIs), 305 human parainfluenza virus types 1 (HPIV1), 154 HPIV2 and 574 HPIV3 strains were isolated from 16,962 nasopharyngeal swabs obtained between 2002 and 2011 at pediatric clinics in Yamagata, Japan. The total isolation frequency for HPIV1-3 was 6...

journal_title：Microbiology and immunology

authors： Mizuta K,Abiko C,Aoki Y,Ikeda T,Itagaki T,Katsushima F,Katsushima Y,Matsuzaki Y,Noda M,Kimura H,Ahiko T

更新日期：2012-12-01 00:00:00

abstract：:Healthy cattle and swine bred in a district of Japan were examined for the presence of Clostridium botulinum in their liver. Liver specimens were cultivated in chopped meat-glucose medium and the cultures were examined for botulinum toxin. In cattle, none of the cultures of 100 liver specimens yielded the toxin. In sw...

journal_title：Microbiology and immunology

authors： Yamakawa K,Kamiya S,Yoshimura K,Nakamura S

更新日期：1992-01-01 00:00:00

abstract：:Inhibition of hemagglutinin (HA) activity in a membrane fraction of Bacteroides gingivalis was examined using various compounds. Leupeptin and anti-pain inhibited the HA activity at nM order. This potency was lost when the aldehyde group of leupeptin was converted to an alcohol moiety. Irreversible protease inhibitors...

journal_title：Microbiology and immunology

authors： Nishikata M,Yoshimura F,Nodasaka Y

更新日期：1989-01-01 00:00:00

abstract：:Virulence of hantavirus strain of SR-11 Seoul virus and Hantaan 76-118 (HTN) of Hantaan virus were compared. Infections of both strains were lethal in newborn mice. However, inoculum required to cause lethal infection was about 4,000 times higher for strain HTN (1.65 x 10(3) PFU/mouse/LD50) than for strain SR-11 (0.36...

journal_title：Microbiology and immunology

authors： Yoo YC,Yoshimatsu K,Yoshida R,Tamura M,Azuma I,Arikawa J

更新日期：1993-01-01 00:00:00

abstract：:The distribution of the antibody to influenza C virus in the dogs and pigs in Yamagata prefecture, Japan was investigated by using three different serological methods: hemagglutination-inhibition (HI), radioimmunoprecipitation (RIP), and immunoblotting. The antibody against influenza C virus glycoprotein (gp88) was de...

journal_title：Microbiology and immunology

authors： Ohwada K,Kitame F,Sugawara K,Nishimura H,Homma M,Nakamura K

更新日期：1987-01-01 00:00:00

abstract：:Previously we reported that mice infected recurrently with live Fusobacterim nucleatum (Fn) synthesize a significant amount of NO between 12 hr and 24 hr after the Fn injection. We now investigated whether the NO has the capability of killing Fn, a gram-negative rod periodontal pathogen. The mice were divided into thr...

journal_title：Microbiology and immunology

authors： Kato C,Mikami M,Suzuki A,Saito K

更新日期：2003-01-01 00:00:00

abstract：:None of the 58 Moraxella catarrhalis strains grew on nutrient agar without sodium chloride supplementation, whereas 49 of 51 commensal Neisseria spp. strains tested did. Growth on nutrient agar without sodium chloride supplementation could be used for screening between M. catarrhalis and commensal Neisseria spp. ...

journal_title：Microbiology and immunology

authors： Chida T,Sawabe E,Ono E,Nishiyama H,Ebina K,Sato K,Watanabe Y,Kuroki T,Okamura N

更新日期：2005-01-01 00:00:00

abstract：:The effects of added maltose on the activities of a preparation of crude glucosyltransferases (GTases) and purified dextransucrase (DS) were investigated to elucidate the inhibition mechanism of maltose on the synthesis of water-insoluble glucan (ISG) in Streptococcus mutans HS-6. Tri- and tetra-saccharides produced b...

journal_title：Microbiology and immunology

authors： Fukui K,Moriyama T

更新日期：1983-01-01 00:00:00

abstract：:The standard products of V(D)J recombination of immunoglobulin and T cell receptor genes are two kinds of DNA junction, a coding joint and a signal joint. TCR delta V-D and D-D signal joints in adult mouse thymocytes were sequenced following PCR amplification. We observed differential nucleotide insertions at the V de...

journal_title：Microbiology and immunology

authors： Kanari Y,Muto M,Yamagishi H

更新日期：2003-01-01 00:00:00

abstract：:Intracellular localization of the wild-type (Spv+), the phoP-constitutively expressed strain (PhoPc), and the spv-deleted strain (Spv-) of Salmonella typhimurium was examined by the use of confocal laser scanning microscopy analysis of immunostained sections of mouse spleens after oral or subcutaneous inoculation. Onl...

journal_title：Microbiology and immunology

authors： Matsui H,Eguchi M,Kikuchi Y

更新日期：2001-01-01 00:00:00

abstract：:Germfree (GF) F344 rats developed severe adjuvant-induced arthritis with a 100% incidence after a single intradermal injection of heat-killed Mycobacterium bovis (BCG). Specific pathogene-free (SPF) rats developed less severe arthritis with a lower incidence. The rats colonized with Escherichia coli or Bacteroides dev...

journal_title：Microbiology and immunology

authors： Kohashi O,Kohashi Y,Takahashi T,Ozawa A,Shigematsu N

更新日期：1985-01-01 00:00:00

abstract：:The lack of a suitable animal model is a major obstacle to developing anti-HIV-1 vaccines. We successfully generated an SIVmac/HIV-1 chimeric virus (SHIV) (designated as NM-3rN) that contains the HIV-1 env gene and is infectious to macaque monkeys. Challenging the vaccinated macaque monkeys with NM-3rN, we developed a...

journal_title：Microbiology and immunology

authors： Haga T,Kuwata T,Ui M,Igarashi T,Miyazaki Y,Hayami M

更新日期：1998-01-01 00:00:00

abstract：:Live attenuated bacteria can be used as a carrier for the delivery of foreign antigens to a host's immune system. The N-terminal domain of SipB, a translocon protein of the type III secretion system of Salmonella enterica serovar Typhimurium, is required for secretion and outer membrane localization. In the present st...

journal_title：Microbiology and immunology

authors： Jang JI,Kim JS,Eom JS,Kim HG,Kim BH,Lim S,Bang IS,Park YK

更新日期：2012-09-01 00:00:00

abstract：:Burkholderia cepacia complex (Bcc) are opportunistic pathogens implicated with nosocomial infections, and high rates of morbidity and mortality, especially in individuals with cystic fibrosis (CF). B. cepacia are naturally resistant to different classes of antibiotics, and can subvert the host innate immune responses ...

journal_title：Microbiology and immunology

authors： Ganesh PS,Vishnupriya S,Vadivelu J,Mariappan V,Vellasamy KM,Shankar EM

更新日期：2020-02-01 00:00:00

abstract：:Hepatitis C virus (HCV) non-structural protein 5A (NS5A) is a multifunctional protein that is involved in the HCV life cycle and pathogenesis. In this study, a host protein(s) interacting with NS5A by tandem affinity purification were searched for with the aim of elucidating the role of NS5A. An NS5A-interacting prote...

journal_title：Microbiology and immunology

authors： Chen M,Gan X,Yoshino K,Kitakawa M,Shoji I,Deng L,Hotta H

更新日期：2016-06-01 00:00:00