Intracellular survival and innate immune evasion of Burkholderia cepacia: Improved understanding of quorum sensing-controlled virulence factors, biofilm, and inhibitors.

abstract：:The phospholipase C (alpha-toxin) gene (plc) of Clostridium perfringens was cloned into pUC19 and the effects of the upstream regions on expression of the plc gene were examined in Escherichia coli JM109. When the 0.7-kb region just upstream of the putative -35 site of the gene was deleted, production of phospholipase...

journal_title：Microbiology and immunology

authors： Toyonaga T,Matsushita O,Katayama S,Minami J,Okabe A

更新日期：1992-01-01 00:00:00

abstract：:Tetracycline (pp-tet), and kanamycin (pp-kan) resistance genes were cloned from a transferable R plasmid of fish pathogen Pasteurella piscicida, and complete nucleotide sequences were determined. The pp-tet was a class D Tet determinant constructed with the tetA resistance gene of 1,182 bp encoding a protein with a de...

journal_title：Microbiology and immunology

authors： Kim EH,Aoki T

更新日期：1994-01-01 00:00:00

abstract：:A new type of immuno-cell therapy called BRM-activated killer (BAK) therapy using non-MHC-restricted lymphocytes, CD56-positive cells, was devised. Peripheral blood lymphocytes were selected by immobilization with anti-CD3 monoclonal antibody and cultured for 2 weeks in the presence of IL-2. Thereafter, they were reac...

journal_title：Microbiology and immunology

authors： Ebina T,Ogama N,Shimanuki H,Kubota T,Isono N

更新日期：2001-01-01 00:00:00

abstract：:Vibrio hollisae strains isolated recently from patients in various locations were examined for the presence of the thermostable direct hemolysin gene (tdh) using nucleic acid hybridization and polymerase chain reaction assays. The results were consistent with the previous finding that all strains of V. hollisae carry ...

journal_title：Microbiology and immunology

authors： Nishibuchi M,Janda JM,Ezaki T

更新日期：1996-01-01 00:00:00

abstract：:Pathogens of the Aspergillus species are frequently seen in deep-seated mycoses. We previously demonstrated that the culture filtrate of Aspergillus fumigatus (CF) has immunosuppressive effects on polymorphonuclear leukocytes (PMNs), which act as the main phagocytes to hyphae of Aspergillus fumigatus (A. fumigatus). B...

journal_title：Microbiology and immunology

authors： Higurashi H,Arai M,Watanabe A,Igari H,Seki N,Kamei K,Kuriyama T

更新日期：2007-01-01 00:00:00

abstract：:The sensitivity of influenza rapid diagnostic tests (IRDTs) currently available in Japan for various influenza virus strains, including human H7N9 and H5N1 isolates, were compared and it was found that all of the IRDTs examined detected these viruses; however, their detection sensitivities differed. ...

journal_title：Microbiology and immunology

authors： Sakai-Tagawa Y,Ozawa M,Yamada S,Uchida Y,Saito T,Takahashi K,Sugaya N,Tashiro M,Kawaoka Y

更新日期：2014-10-01 00:00:00

abstract：:The Clostridium perfringens strain 13 genome contains two genes (fbpA, fbpB) that encode putative Fbp. Both rFbpA and rFbpB were purified and their reactivity with human serum Fn was analyzed. To determine the region of the Fn molecule recognized by rFbp, a plate binding assay using N-terminal 70-kDa peptide, III1-C p...

journal_title：Microbiology and immunology

authors： Yamasaki T,Hitsumoto Y,Katayama S,Nogami Y

更新日期：2010-04-01 00:00:00

abstract：:Unmethylated CpG-ODN are known to enhance Th1-type immune response. However, optimal sequences of CpG-ODN for activating Th1-type immune cells vary among species. It is necessary to identify the effective CpG-ODN sequences in each species. In the present study, in order to identify the sequences of CpG-ODN that produc...

journal_title：Microbiology and immunology

authors： Satoh R,Kotake M,Takano T,Motokawa K,Gemma T,Watanabe R,Arai S,Hohdatsu T

更新日期：2011-03-01 00:00:00

abstract：:Murine γδ T cells develop as the first T-cell lineage within the fetal thymus and disproportionately localize in mucosal tissues such as lung, skin, uterus, and intestine of adult mice. These unique developmental features and distribution patterns of γδ T cells enable rapid functioning against various insults from pat...

journal_title：Microbiology and immunology

authors： Shibata K

更新日期：2012-04-01 00:00:00

abstract：:The in vitro activity of ofloxacin, a new pyridone-carboxylic acid, against 11 strains of Chlamydia trachomatis and six strains of Chlamydia psittaci was determined. All test strains of both species were inhibited by 0.39 micrograms of ofloxacin per ml. The antichlamydial activity of ofloxacin was comparable to that o...

journal_title：Microbiology and immunology

authors： Hirai K,Une T

更新日期：1986-01-01 00:00:00

abstract：:A protease was purified from a strain of Vibrio vulnificus isolated from the blood of a septicemic human. The vibrio was cultured in bacto peptone-yeast extract medium, and the protease was purified by a purification procedure including ultrafiltration of the culture supernatant with an Amicon YM 5 membrane, diethylam...

journal_title：Microbiology and immunology

authors： Miyoshi N,Shimizu C,Miyoshi S,Shinoda S

更新日期：1987-01-01 00:00:00

abstract：:A solubilized sheep red blood cell (SRBC) antigen (supernatant fraction obtained by centrifuging 10(7)-2 x 108 sonicated SRBC at 6 x 10(4) g for 30 min [Sup-SRBC]), whose ability to inhibit anti-SRBC plaque formation was 70% of that of the original sonicated SRBC, was unable to elicit a detectable antibody response in...

journal_title：Microbiology and immunology

authors： Nagase F,Nakashima I,Nagase N,Kato N

更新日期：1982-01-01 00:00:00

abstract：:Thrombin-activatable fibrinolysis inhibitor (TAFI) is an anaphylatoxin-inactivating enzyme generated by proteolytic cleavage of its zymogen, and is the same enzyme as that first designated by our group as procarboxypeptidase R (proCPR). TAFI in plasma is presumed to influence vascular disease in its role as a fibrinol...

journal_title：Microbiology and immunology

authors： Akatsu H,Ishiguro M,Ogawa N,Kanesaka T,Okada N,Yamamoto T,Campbell W,Okada H

更新日期：2007-01-01 00:00:00

abstract：:The immune responses of individuals exposed to Leishmania major were evaluated and compared with those of non-exposed volunteers. Forty-one patients with active lesion(s), 43 healed individuals, 15 vaccinees 1 month or 1 year post vaccination, and 15 non-exposed volunteers were studied. Leishmanin skin test (LST) resp...

journal_title：Microbiology and immunology

authors： Alimohammadian MH,Darabi H,Malekzadeh S,Mahmoodzadeh-Niknam H,Ajdary S,Khamesipour A,Bahonar A,Mofarrah A

更新日期：2007-01-01 00:00:00

abstract：:Rapid western blot (WB) procedure for an abnormal isoform of prion protein (PrP(Sc) ) detection in lymphoid tissues was established and has been applied to the surveillance of fallen stock. In this program, brain and palatal tonsil were examined by WB and three cases of sheep scrapie were detected. While one clinicall...

journal_title：Microbiology and immunology

authors： Shimada K,Hayashi HK,Ookubo Y,Iwamaru Y,Imamura M,Takata M,Schmerr MJ,Shinagawa M,Yokoyama T

更新日期：2005-01-01 00:00:00

abstract：:A cytotoxic T lymphocyte (CTL) response to dengue virus-infected target cells is described. Effector cells were generated in an in vitro secondary culture and appeared to be T cells possessing both the Lyt 1.1 and Lyt 2.1 surface antigens. A stronger CTL response was noted with the H-2k haplotype compared to H-2d, and...

journal_title：Microbiology and immunology

authors： Pang T,Devi S,Blanden RV,Lam SK

更新日期：1988-01-01 00:00:00

abstract：:There are many examples of probiotic effects of various lactic bacteria on enteropathogens. In this study, Lactobacillus strains (L. rhamnosus, L. gasseri, L. casei and L. plantarum) were tested in an in vitro model of enterohemorrhagic Escherichia coli (EHEC) infection of a human colon epithelial cell line, C2BBe1. W...

journal_title：Microbiology and immunology

authors： Hirano J,Yoshida T,Sugiyama T,Koide N,Mori I,Yokochi T

更新日期：2003-01-01 00:00:00

abstract：:To determine the infection source of a sporadic Legionella pneumonia case associated with a hot spring bath, we used five molecular methods, including repetitive element polymerase chain reaction (rep-PCR), arbitrarily primed PCR (AP-PCR), ribotyping, restriction endonuclease analysis (REA), and macrorestriction endon...

journal_title：Microbiology and immunology

authors： Miyamoto H,Jitsurong S,Shiota R,Maruta K,Yoshida S,Yabuuchi E

更新日期：1997-01-01 00:00:00

abstract：:Experiments were performed to investigate the resistance of the host due to antibody-mediated mechanisms to herpes simplex virus (HSV) infection. Transfer of hyperimmune anti-HSV mouse serum inhibited the development of skin lesions and prolonged the survival of lethally HSV-infected nude mice. Relatively high concent...

journal_title：Microbiology and immunology

authors： Hayashida I,Nagafuchi S,Hayashi Y,Kino Y,Mori R,Oda H,Ohtomo N,Tashiro A

更新日期：1982-01-01 00:00:00

abstract：:CD4(+) T cells play critical roles in protection against the blood stage of malarial infection; however, their uncontrolled activation can be harmful to the host. In this study, in which rodent models of Plasmodium parasites were used, the expression of inhibitory receptors on activated CD4(+) T cells and their cytoki...

journal_title：Microbiology and immunology

authors： Doe HT,Kimura D,Miyakoda M,Kimura K,Akbari M,Yui K

更新日期：2016-02-01 00:00:00

abstract：:Hepatitis C virus (HCV) non-structural protein 5B (NS5B) is an RNA replicase. We expressed full-length NS5B (591 amino acid residues) in Escherichia coli as a fusion protein with maltose binding protein (MBP-NS5B). MBP-NS5B was recovered in the soluble fraction after centrifugation at 40,000 x g and affinity-purified ...

journal_title：Microbiology and immunology

authors： Tanaka T,Sugiyama K,Ikeda M,Naganuma A,Nozaki A,Saito M,Shimotohno K,Kato N

更新日期：2000-01-01 00:00:00

abstract：:Fibronectin is known to be an important factor in colonization by Staphylococcus aureus of host tissues as well as other extracellular matrix proteins such as collagen and laminin. We investigated the effect of fibronectin on the phagocytosis of the S. aureus Cowan I strain by macrophages and of coagulase-negative sta...

journal_title：Microbiology and immunology

authors： Shinji H,Sakurada J,Seki K,Murai M,Masuda S

更新日期：1998-01-01 00:00:00

abstract：:To detect neutralization-relevant antibodies against 3 types of poliovirus (PV) without using tissue cultures and live viruses, an enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibody-binding inhibition was evaluated using sera from 80 vaccinated Japanese children and 60 Pakistani poliomyelitis patie...

journal_title：Microbiology and immunology

authors： Hashido M,Horie H,Abe S,Doi Y,Hashizume S,Agboatwalla M,Isomura S,Nishio O,Hagiwara A,Inouye S

更新日期：1999-01-01 00:00:00

abstract：:Dengue virus multiplication in cultures of a murine myelomonocytic cell line (WEHI-3) as well as mouse peritoneal macrophages was enhanced by treatment of the cells with lipophilic derivatives of muramyl peptides for 2 or 3 days before virus inoculation, but not for 2 hr before virus inoculation or during the adsorpti...

journal_title：Microbiology and immunology

authors： Hotta H,Sanchez LF,Takada H,Homma M,Kotani S

更新日期：1985-01-01 00:00:00

abstract：:Pneumocystis spp. are opportunistic fungal pathogens that are closely associated with severe pneumonia and pulmonary complications in patients with impaired immunity. In this study, the antigenic epitopes of the gene encoding the 55 kDa antigen fragment of Pneumocystis (p55), which may play an important role in Pneumo...

journal_title：Microbiology and immunology

authors： Fan H,Guo JY,Ma SL,Zhang N,An CL

更新日期：2016-06-01 00:00:00

abstract：:Immunological analyses of lipopolysaccharides (LPS) isolated from Proteus strains OX2, OX19, and OXK used as antigens of Weil-Felix (WF) test, were performed by quantitative agglutination, enzyme-linked immunosorbent assay (ELISA), and immunoblotting. Antisera against LPS and whole cells (WC) of the three Proteus stra...

journal_title：Microbiology and immunology

authors： Amano K,Mizushiri S,Fujii S,Fukushi K,Suto T

更新日期：1990-01-01 00:00:00

abstract：:The US3 of HSV encodes a serine/threonine protein kinase that is highly conserved among members of the alphaherpesviruses. It is an accessory gene that is not required for viral replication in cultured cells but appears essential for viral survival in humans. Although accumulating in vitro evidence suggested that the ...

journal_title：Microbiology and immunology

authors： Mori I

更新日期：2012-06-01 00:00:00

abstract：:The in vitro effect of the heat-labile toxin (HLT) of Bordetella parapertussis on HeLa, baby hamster kidney (BHK), chinese hamster ovary (CHO), myeloma (Pa-NS-1), human embryonic lung (HEL-R66) cells, erythrocytes, adipocytes and lymphocytes from guinea pigs, mice or rats, or aortic smooth muscle cells from pigs or gu...

journal_title：Microbiology and immunology

authors： Endoh M,Nagai M,Ueda T,Yoshida Y,Nakase Y

更新日期：1988-01-01 00:00:00

abstract：:To study the role of Moraxella (subgenus Branhamella) catarrhalis (B. catarrhalis) adherence to airway cells in lower respiratory tract infections, the in vitro attachments of B. catarrhalis to upper airway (oropharyngeal) and lower airway (bronchial) epithelial cells were compared. The adherence of 4 strains (1 nonfi...

journal_title：Microbiology and immunology

authors： Rikitomi N,Ahmed K,Nagatake T

更新日期：1997-01-01 00:00:00

abstract：:Antimicrobial peptides contribute to innate host defense against a number of bacteria and fungal pathogens. Some of antimicrobial synthetic peptides were systemically administered in vivo; however, effective protection has so far not been obtained because the effective dose of peptides in vivo seems to be very high, o...

journal_title：Microbiology and immunology

authors： Hamamoto K,Kida Y,Zhang Y,Shimizu T,Kuwano K

更新日期：2002-01-01 00:00:00