Abstract:
:Tetracycline (pp-tet), and kanamycin (pp-kan) resistance genes were cloned from a transferable R plasmid of fish pathogen Pasteurella piscicida, and complete nucleotide sequences were determined. The pp-tet was a class D Tet determinant constructed with the tetA resistance gene of 1,182 bp encoding a protein with a deduced molecular mass of 41 kDa and the tetR repressor gene of 654 bp encoding a product of 24 kDa. The pp-tet was highly homologous to the tet(D) of plasmid RA1 isolated from Aeromonas hydrophila with two nucleotide differences in the tetR, and of plasmid pIP173 from Salmonella ordonez with two nucleotide differences in the tetA. The pp-kan contained 813 bp encoding a 31 kDa protein of 271 amino acids, and was classified into type aph-Ic. It was identical to the aphA7 in the IAB operon of pBWH77, in which was originally found an isolate of Klebsiella pneumoniae, in its nucleotide sequences and hybrid promoter construction. The genes were connected by an insertion sequence IS26 of 820 bp, and were flanked by repeated copies in direct orientation at the 3' flanking region of the pp-tetA and in inverted orientation at the 3' flanking region of the pp-kan. The genetic elements are organized like a complex transposon by close linkage of the IS26 and the pp-tet and -kan.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Kim EH,Aoki Tdoi
10.1111/j.1348-0421.1994.tb01741.xsubject
Has Abstractpub_date
1994-01-01 00:00:00pages
31-8issue
1eissn
0385-5600issn
1348-0421journal_volume
38pub_type
杂志文章abstract::Biofilm formation can cause refractory urinary tract infections (UTIs) in dogs; however, minimum biofilm eradication concentrations (MBECs) of veterinary drugs against canine uropathogens remain to be investigated. In this study, the MBECs of orbifloxacin (OBFX), trimethoprim-sulfamethoxazole (TMS) and amoxicillin/cla...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12461
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1994.tb01756.x
更新日期:1994-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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doi:10.1111/j.1348-0421.1999.tb02396.x
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journal_title:Microbiology and immunology
pub_type: 杂志文章,评审
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1981.tb00099.x
更新日期:1981-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/1348-0421.12588
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1996.tb01080.x
更新日期:1996-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1985.tb00864.x
更新日期:1985-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2008.00020.x
更新日期:2008-03-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1991.tb01574.x
更新日期:1991-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1995.tb03255.x
更新日期:1995-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2005.tb03660.x
更新日期:2005-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1348-0421.1984.tb00679.x
更新日期:1984-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12290
更新日期:2015-09-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1988.tb01436.x
更新日期:1988-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1998.tb02351.x
更新日期:1998-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1978.tb00355.x
更新日期:1978-01-01 00:00:00
abstract::Lancefield group C Streptococcus dysgalactiae causes infections in farmed fish. Here, the genome of S. dysgalactiae strain kdys0611, isolated from farmed amberjack (Seriola dumerili) was sequenced. The complete genome sequence of kdys0611 consists of a single chromosome and five plasmids. The chromosome is 2,142,780 b...
journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2019-07-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2001-01-01 00:00:00
abstract::The effect of fatty acids on Mycobacterium smegmatis was examined in vitro at pH 5.0 to 7.0 to determine the role of fatty acids in the intracellular killing of mycobacteria. Unsaturated fatty acids showed strong bactericidal activity in low concentrations (0.005 to 0.02 mM), whereas saturated fatty acids, except for ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1985.tb00811.x
更新日期:1985-01-01 00:00:00
abstract::There is a deficiency in the data concerning the role of hMPV in lower respiratory tract infections in adults, and until now there has been no data available regarding the prevalence of hMPV in adults in our region. In the present study the association of hMPV with varieties of lower respiratory tract disorders in imm...
journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2009-11-01 00:00:00
abstract::None of the 58 Moraxella catarrhalis strains grew on nutrient agar without sodium chloride supplementation, whereas 49 of 51 commensal Neisseria spp. strains tested did. Growth on nutrient agar without sodium chloride supplementation could be used for screening between M. catarrhalis and commensal Neisseria spp. ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00