Complete genome sequence and characterization of virulence genes in Lancefield group C Streptococcus dysgalactiae isolated from farmed amberjack (Seriola dumerili).

abstract：:Live attenuated bacteria can be used as a carrier for the delivery of foreign antigens to a host's immune system. The N-terminal domain of SipB, a translocon protein of the type III secretion system of Salmonella enterica serovar Typhimurium, is required for secretion and outer membrane localization. In the present st...

journal_title：Microbiology and immunology

authors： Jang JI,Kim JS,Eom JS,Kim HG,Kim BH,Lim S,Bang IS,Park YK

更新日期：2012-09-01 00:00:00

abstract：:We isolated a filamentous bacteriophage, VfO4K68, from the pandemic Vibrio parahaemolyticus strain belonging to 04:K68 serovar. The VfO4K68 DNA lacked a 1,893-bp fragment present in that of the distinctive region of f237, a filamentous phage isolated from a pandemic 03:K6 strain (Nasu, H. et al., J. Clin. Microbiol., ...

journal_title：Microbiology and immunology

authors： Chan B,Miyamoto H,Taniguchi H,Yoshida S

更新日期：2002-01-01 00:00:00

abstract：:In order to assess the role of the route of immunization on the immunogenicity of killed Salmonella vaccine, mice were immunized with killed S. enteritidis by intraperitoneal (i.p.) and intradermal (i.d.) routes. Whereas the former was non-immunogenic, the i.d. immunization generated an excellent delayed-type hypersen...

journal_title：Microbiology and immunology

authors： George A,Shroff KE,Rath S,Ghosh SN,Sengupta SR,Kamat RS

更新日期：1989-01-01 00:00:00

abstract：:Herpes simplex virus (HSV) class-specific antibody responses after primary genital herpes infection were evaluated with an enzyme-linked immunosorbent assay (ELISA) in 16 patients treated with acyclovir (ACV), given orally, and 17 patients treated with adenine arabinoside (Ara-A), given topically. ACV significantly su...

journal_title：Microbiology and immunology

authors： Kawana T,Hashido M,Koizumi Y

更新日期：1995-01-01 00:00:00

abstract：:The effect of fatty acids on Mycobacterium smegmatis was examined in vitro at pH 5.0 to 7.0 to determine the role of fatty acids in the intracellular killing of mycobacteria. Unsaturated fatty acids showed strong bactericidal activity in low concentrations (0.005 to 0.02 mM), whereas saturated fatty acids, except for ...

journal_title：Microbiology and immunology

authors： Kanetsuna F

更新日期：1985-01-01 00:00:00

abstract：:Accurate and rapid detection of carbapenemases and identification of their types in Enterobacteriaceae are both still major challenges for clinical laboratories in attempting to prevent the intrusion and transmission of carbapenemase-producing Enterobacteriaceae. This study aimed to evaluate the performance of the MAS...

journal_title：Microbiology and immunology

authors： Ohsaki Y,Kubo R,Hobson J,Davies M,Osaka S,Hayashi W,Taniguchi Y,Koide S,Nagano Y,Nagano N

更新日期：2018-01-01 00:00:00

abstract：:Alkaline phosphatase-conjugated oligonucleotide probes were developed to detect the gene coding for Vero toxin 1 (VT1) and Vero toxin 2 (VT2). Using these probes, 3 hr was enough to detect VT genes when suspicious colonies of enterohaemorrhagic Escherichia coli (EHEC) were obtained on an agar plate. The results of a h...

journal_title：Microbiology and immunology

authors： Yoh M,Takagi K,Eda J,Ohtomo M,Takarada Y,Shibata S,Honda T

更新日期：1997-01-01 00:00:00

abstract：:The Wilms' tumor gene WT1 is overexpressed in various types of solid tumors, including lung and breast cancer and WT1 protein is a tumor antigen for these malignancies. In phase I clinical trials of WT1 peptide-based cancer immunotherapy, two patients with advanced lung cancer were intradermally injected with 0.3 mg o...

journal_title：Microbiology and immunology

authors： Tsuboi A,Oka Y,Osaki T,Kumagai T,Tachibana I,Hayashi S,Murakami M,Nakajima H,Elisseeva OA,Fei W,Masuda T,Yasukawa M,Oji Y,Kawakami M,Hosen N,Ikegame K,Yoshihara S,Udaka K,Nakatsuka S,Aozasa K,Kawase I,Sugiyama H

更新日期：2004-01-01 00:00:00

abstract：:The activity of glucose dehydrogenase present in resting spores of Bacillus subtilis varied strikingly with the conditions for disrupting the spores by sonic treatment, namely, the time and strength of sonication, and the type and pH of the solution used for suspending the spores. When the resting spores were sonicate...

journal_title：Microbiology and immunology

authors： Tochikubo K,Yasuda Y

更新日期：1983-01-01 00:00:00

abstract：:A reversed passive latex agglutination (RPLA) assay for detecting Clostridium difficile toxin A is presented. Purified monoclonal antibody (mAb 37B5) was used for latex sensitization. The culture supernatants of 93 strains of C. difficile were tested by RPLA assay and the results compared with those of a commercially ...

journal_title：Microbiology and immunology

authors： Toma C,Nakamura S,Kamiya S,Nakasone N,Iwanaga M

更新日期：1999-01-01 00:00:00

abstract：:A 9.8-kbp DNA fragment which contained a neurotoxin gene and its upstream region was cloned from Clostridium botulinum type D strain CB-16. Nucleotide sequencing of the fragment revealed that genes encoding for hemagglutinin (HA) subcomponents and one for a nontoxic-nonhemagglutinin (NTNH) component were located upstr...

journal_title：Microbiology and immunology

authors： Ohyama T,Watanabe T,Fujinaga Y,Inoue K,Sunagawa H,Fujii N,Inoue K,Oguma K

更新日期：1995-01-01 00:00:00

abstract：:A VeraCode-allele-specific primer extension (ASPE) method was applied to the detection and genotyping of human papillomavirus (HPV)-DNA. Oligonucleotide primers containing HPV-type-specific L1 sequences were annealed to HPV-DNA amplified by PGMY-PCR, followed by ASPE to label the DNA with biotinylated nucleotides. The...

journal_title：Microbiology and immunology

authors： Kitamura-Muramatsu Y,Kusumoto-Matsuo R,Kondo K,Mori S,Saito S,Tsukahara Y,Kukimoto I

更新日期：2012-02-01 00:00:00

abstract：:Tetrads-forming mutant MT cells of Micrococcus luteus, both treated with chemical reagents and non-treated, were observed with a scanning electron microscope (SEM). The agglutinability of the cells with antiserum containing anti-teichuronic acid antibody was examined. The binding of protein A-gold particles to the cel...

journal_title：Microbiology and immunology

authors： Monodane T,Tokunaga M,Torii M

更新日期：1990-01-01 00:00:00

abstract：:This study investigated the mechanisms of resistance of 36 quinolone-resistant Salmonella Typhimurium strains isolated from outpatients with infectious diarrhea in Beijing Tian Tan Hospital between 2013 and 2015. The resistance spectrum of the 36 strains was measured using a broth dilution method. Class 1 integrons ha...

journal_title：Microbiology and immunology

authors： Yuan J,Guo W

更新日期：2017-04-01 00:00:00

abstract：:Burkholderia pseudomallei, a pathogenic gram-negative bacterium, causes the severe human disease melioidosis. This organism can survive in eukaryotic host cells by escaping reactive oxygen species via the regulation of stress responsive sigma factors, including RpoS. In B. pseudomallei, RpoS has been reported to play ...

journal_title：Microbiology and immunology

authors： Chutoam P,Charoensawan V,Wongtrakoongate P,Kum-Arth A,Buphamalai P,Tungpradabkul S

更新日期：2013-09-01 00:00:00

abstract：:Antibodies to fibronectin-binding proteins (FnBPs) of Staphylococcus aureus, including binding domain of FnBPA, the D region, or the A-C regions of FnBPB were produced in rabbits and mice. These antibodies were used to characterize cell-associated FnBPs of S. aureus strain Cowan I, S. aureus strain U320 and a coagulas...

journal_title：Microbiology and immunology

authors： Sakata N,Rozalska B,Wadström T

更新日期：1994-01-01 00:00:00

abstract：:The attachment of Mycoplasma pulmonis m53 organisms to mouse and rat synovial cells was examined by using the organisms and the synovial cells treated in various ways. M. pulmonis treated with trypsin attached to the synovial cells, but the organisms treated with pronase, formaldehyde, glutaraldehyde, or heat did not....

journal_title：Microbiology and immunology

authors： Araake M,Yayoshi M,Yoshioka M

更新日期：1985-01-01 00:00:00

abstract：:Sera from 3,158 individuals living in northern Egypt were tested for the presence of antibodies against human T-lymphotropic virus type I (HTLV-I) by the newly developed particle agglutination (PA) test. Ten sera gave a positive reaction in the PA test. Eight of these sera were examined further by Western blotting and...

journal_title：Microbiology and immunology

authors： el-Farrash MA,Badr MF,Hawas SA,el-Nashar NM,Imai J,Komoda H,Hinuma Y

更新日期：1988-01-01 00:00:00

abstract：:Escherichia coli isolates from calves with diarrhea (1 day to 8 weeks old, 140 individuals) were surveyed for the three immunologically distinct fimbrial adhesins FY, 31A, and K99. Of a total of 1,370 strains isolated, 96 (7.0%), 34 (2.5%), 75 (5.5%), and 13 (0.9%) were identified as FY+, 31A+, FY+.31A+, and K99+, res...

journal_title：Microbiology and immunology

authors： Shimizu M,Sakano T,Yamamoto J,Kitajima K

更新日期：1987-01-01 00:00:00

abstract：:Tetracycline (pp-tet), and kanamycin (pp-kan) resistance genes were cloned from a transferable R plasmid of fish pathogen Pasteurella piscicida, and complete nucleotide sequences were determined. The pp-tet was a class D Tet determinant constructed with the tetA resistance gene of 1,182 bp encoding a protein with a de...

journal_title：Microbiology and immunology

authors： Kim EH,Aoki T

更新日期：1994-01-01 00:00:00

abstract：:A CpG-enriched recombinant plasmid (pUC18-CpG) as an adjuvant of FMD killed vaccine was tested for immunization and vaccination challenge in a porcine model. Our preliminary results had indicated that the recombinant plasmid could enhance the humoral immune response triggered by the traditional oil-adjuvant vaccine af...

journal_title：Microbiology and immunology

authors： Guo X,Jia H,Zhang Q,Yuan W,Zhu G,Xin T,Zhu H,Sánchez-Vizcaíno JM

更新日期：2012-05-01 00:00:00

abstract：:Enterovirus 71 (EV71) is the predominant pathogen for severe hand, foot, and mouth disease (HFMD) in children younger than 5 years, and currently no effective drugs are available for EV71. Thus, there is an urgent need to develop new drugs for the control of EV71 infection. In this study, LJ04 was extracted from Lamin...

journal_title：Microbiology and immunology

authors： Li Z,Cui B,Liu X,Wang L,Xian Q,Lu Z,Liu S,Cao Y,Zhao Y

更新日期：2020-03-01 00:00:00

abstract：:Electron microscopy revealed multi-layered membranes within the cytoplasmic inclusion (accumulation of nucleocapsids) produced by rabies virus. When infected BHK cells were maintained at 31 C, an enhancement in production of these membranes occurred in approximately 60% of inclusion-containing cells. Multi-layered mem...

journal_title：Microbiology and immunology

authors： Tanabe K,Matsumoto S

更新日期：1979-01-01 00:00:00

abstract：:Absorption of a pooled human gamma globulin preparation with acetone-treated measles virus-infected cells removed all antibodies to measles virus antigens except a portion of the antibody to the fusion (F) protein. The residual anti-F antibody had hemolysis-inhibiting and virus-neutralizing activities, inhibited sprea...

journal_title：Microbiology and immunology

authors： Sato TA,Kohama T,Sugiura A

更新日期：1989-01-01 00:00:00

abstract：:Biofilm formation is an important phenotype associated with chronic Pseudomonas aeruginosa infections. In the present study, a total of 48 P. aeruginosa strains isolated from clinical specimens were examined for their biofilm-forming ability using a microtiter plate method. The different biofilm-forming abilities were...

journal_title：Microbiology and immunology

authors： Yang W,Shi L,Jia WX,Yin X,Su JY,Kou Y,Yi X,Shinoda S,Miyoshi S

更新日期：2005-01-01 00:00:00

abstract：:A chimeric PCV1-2 clone containing the PCV2 capsid gene cloned into the backbone of the nonpathogenic PCV1 genome was recently generated based on PCV2 and PCV1 strains isolated in China. The efficacy of this available candidate inactivated vaccine was evaluated by subjecting conventional pigs to intramuscular immuniza...

journal_title：Microbiology and immunology

authors： Xujie L,Xiaobo W,Yi S,Jing F,Song G,Xiufan L

更新日期：2011-04-01 00:00:00

abstract：:Macrophages are extremely heterogeneous mononuclear phagocytes widely distributed throughout the body. They play unique roles in each organ where they reside. Among macrophage subsets, red pulp macrophages (RPMs) that localize in the splenic red pulp, are critical for maintenance of blood homeostasis by actively phago...

journal_title：Microbiology and immunology

authors： Kurotaki D,Uede T,Tamura T

更新日期：2015-02-01 00:00:00

abstract：:Importance of the overexpression of AcrAB efflux pumps in the low-level resistance of pathogens to antimicrobials requires a better understanding of the AcrAB regulation. The goal of the present research was to study the transcription of acrAB, as well as the genes that play a role in its regulation in Salmonella ente...

journal_title：Microbiology and immunology

authors： Hartog E,Ben-Shalom L,Shachar D,Matthews KR,Yaron S

更新日期：2008-12-01 00:00:00

abstract：:Children with renal diseases are typically treated with immunosuppressive drugs, which place them at high risk of reactivation of the BK virus (BKV). Currently, little is known about the impact of immunosuppressive drugs on the kinetics of urinary shedding of BKV and viral reactivation in pediatric patients with renal...

journal_title：Microbiology and immunology

authors： Yamamoto Y,Morooka M,Ihira M,Yoshikawa T

更新日期：2015-01-01 00:00:00

abstract：:Chikungunya virus (CHIKV) was purified and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis in discontinuous buffer systems. Three bands were revealed by staining with Coomassie blue; two of them (E1 and E2) were associated with the membrane, and one (C) with the core. Their molecular weights wer...

journal_title：Microbiology and immunology

authors： Konishi E,Hotta S

更新日期：1980-01-01 00:00:00