Evaluation of the biofilm-forming ability and genetic typing for clinical isolates of Pseudomonas aeruginosa by enterobacterial repetitive intergenic consensus-based PCR.

abstract：:Murine γδ T cells develop as the first T-cell lineage within the fetal thymus and disproportionately localize in mucosal tissues such as lung, skin, uterus, and intestine of adult mice. These unique developmental features and distribution patterns of γδ T cells enable rapid functioning against various insults from pat...

journal_title：Microbiology and immunology

authors： Shibata K

更新日期：2012-04-01 00:00:00

abstract：:Virulence of Coxsackie virus B3 (CB3) was compared in mice between strain SK-74 isolated from a patient and strain T-70 isolated from a healthy child as well as between prototype strain Nancy and its mouse-passaged derivative strain PMH. Strain SK-74 showed a high mortality (40-80%), while strain T-70 did not induce d...

journal_title：Microbiology and immunology

authors： Komatsu T,Hashimoto I,Kohara T

更新日期：1983-01-01 00:00:00

abstract：:Live attenuated bacteria can be used as a carrier for the delivery of foreign antigens to a host's immune system. The N-terminal domain of SipB, a translocon protein of the type III secretion system of Salmonella enterica serovar Typhimurium, is required for secretion and outer membrane localization. In the present st...

journal_title：Microbiology and immunology

authors： Jang JI,Kim JS,Eom JS,Kim HG,Kim BH,Lim S,Bang IS,Park YK

更新日期：2012-09-01 00:00:00

abstract：:Cholesterol is necessary for the conversion of Vibrio cholerae hemolysin (VCH) monomers into oligomers in liposome membranes. Using different sterols, we determined the stereochemical structures of the VCH-binding active groups present in cholesterol. The VCH monomers are bound to cholesterol, diosgenin, campesterol, ...

journal_title：Microbiology and immunology

authors： Ikigai H,Otsuru H,Yamamoto K,Shimamura T

更新日期：2006-01-01 00:00:00

abstract：:The test for susceptibility to kanamycin is useful for differentiating between the genera Rhodococcus and Nocardia. All rhodococci except R. equi, R. erythropolis, and R. aurantiacus are susceptible to kanamycin, whereas all nocardiae except N. otitidis-caviarum are resistant to kanamycin. Tests for susceptibility to ...

journal_title：Microbiology and immunology

authors： Tsukamura M

更新日期：1988-01-01 00:00:00

abstract：:Human adenoviruses (HAdVs) are an important cause of acute gastroenteritis in children. However, few studies on the epidemiology or types of HAdVs associated with acute gastroenteritis have been conducted in Korea. Therefore, in the present study, the incidence of HAdV in 2064 stool samples from Korean children hospit...

journal_title：Microbiology and immunology

authors： Lee JI,Lee GC,Chung JY,Han TH,Lee YK,Kim MS,Lee CH

更新日期：2012-08-01 00:00:00

abstract：:Using a new plaque cloning technique, we obtained unique Chlamydia trachomatis strains which were confirmed to be free of the 7.5-kb common plasmid and glycogen in inclusions. The in vitro susceptibility of these strains to various chemotherapeutic agents was tested by comparison with their parent strains and clinical...

journal_title：Microbiology and immunology

authors： Miyashita N,Matsumoto A,Matsushima T

更新日期：2000-01-01 00:00:00

abstract：:Inhibition of hemagglutinin (HA) activity in a membrane fraction of Bacteroides gingivalis was examined using various compounds. Leupeptin and anti-pain inhibited the HA activity at nM order. This potency was lost when the aldehyde group of leupeptin was converted to an alcohol moiety. Irreversible protease inhibitors...

journal_title：Microbiology and immunology

authors： Nishikata M,Yoshimura F,Nodasaka Y

更新日期：1989-01-01 00:00:00

abstract：:Bacteria of Stenotrophomonas maltophilia have been isolated with increasing frequency from the airways of cystic fibrosis (CF) patients, usually following P. aeruginosa infections, but their adherence to human epithelial respiratory cells has never been investigated. In this study, various S. maltophilia strains were ...

journal_title：Microbiology and immunology

authors： De Vidipó LA,De Marques EA,Puchelle E,Plotkowski MC

更新日期：2001-01-01 00:00:00

abstract：:We constructed a plasmid containing bovine leukemia virus (BLV)-tax gene driven by SR alpha promoter, designated as pME-BLVtax, to activate the promoter of the long terminal repeat (LTR) of BLV in various tumor cells. Activation of the promoter of BLV-LTR by pME-BLVtax was confirmed by luciferase assay. When the cells...

journal_title：Microbiology and immunology

authors： Tana,Watarai S,Aida Y,Tajima S,Kakidani H,Onuma M,Kodama H

更新日期：2001-01-01 00:00:00

abstract：:Leptospire lipopolysaccharide (LPS) stimulated the adherence of polymorphonuclear neutrophils (PMNs) to human umbilical vein endothelial cells (HUVEC). Enhanced PMN adherence in response to leptospire LPS can be mediated by platelet-activator-factor (PAF), because a PAF antagonist reduced adherence. Leptospire LPS als...

journal_title：Microbiology and immunology

authors： Isogai E,Hirose K,Kimura K,Hayashi S,Kubota T,Fujii N,Isogai H

更新日期：1997-01-01 00:00:00

abstract：:Four N-acetylchito-oligosaccharides, from tetra-N-acetylchitotetraose (NACOS-4) to hepta-N-acetylchitoheptaose (NACOS-7), were found to increase the number of peritoneal exudate cells (PEC) in male BALB/c mice after 3 hr intraperitoneal administration of 50 mg/kg of each oligosaccharide. The number of attracted cells,...

journal_title：Microbiology and immunology

authors： Suzuki K,Tokoro A,Okawa Y,Suzuki S,Suzuki M

更新日期：1986-01-01 00:00:00

abstract：:ELISA was used to measure the amount and avidity of IgG antibodies to exotoxin A (ExA) and 7 Fisher's immunotypes of lipopolysaccharide (LPS) in the sera of 13 patients with mild or moderate Pseudomonas aeruginosa infections. Changes in the specificity of tested sera during the course of infection were demonstrated. A...

journal_title：Microbiology and immunology

authors： Trafny EA,Grzybowski J,Patzer J,Popiel D,Wrembel-Wargocka J,Dzierzanowska D,Zawistowska-Marciniak I,Kobus K

更新日期：1991-01-01 00:00:00

abstract：:A simple method for the separation and identification of Bacteroides fragilis from other Bacteroides species through the use of oxgall-impregnated filter paper disks is described. As in the antibiotic sensitivity test, filter paper disks containing defined amounts of bile salt were placed on a lawn of bacterial cells ...

journal_title：Microbiology and immunology

authors： Yamazaki E,Sugimoto K,Niwano K,Okada J

更新日期：1982-01-01 00:00:00

abstract：:A chimeric PCV1-2 clone containing the PCV2 capsid gene cloned into the backbone of the nonpathogenic PCV1 genome was recently generated based on PCV2 and PCV1 strains isolated in China. The efficacy of this available candidate inactivated vaccine was evaluated by subjecting conventional pigs to intramuscular immuniza...

journal_title：Microbiology and immunology

authors： Xujie L,Xiaobo W,Yi S,Jing F,Song G,Xiufan L

更新日期：2011-04-01 00:00:00

abstract：:Two gram-negative, catalase-negative, oxidase-positive strains (PAGU 1467T and PAGU 1468) isolated from patients with infective endocarditis were investigated to determine their taxonomic status. 16S rRNA gene sequence analysis indicated that the two strains were members of the Bergeyella-Chryseobacterium-Riemerella b...

journal_title：Microbiology and immunology

authors： Tomida J,Fujiwara N,Naka T,Morita Y,Sawabe E,Tojo N,Kikuchi K,Kawamura Y

更新日期：2019-03-01 00:00:00

abstract：:The colH gene encoding 116-kDa collagenase of Clostridium histolyticum (cColH) was cloned into an Escherichia coli-Bacillus subtilis shuttle vector to develop a method for purification of recombinant collagenase (rColH). When plasmid pJCM310 containing the colH gene was introduced into B. subtilis DB104 and the transf...

journal_title：Microbiology and immunology

authors： Jung CM,Matsushita O,Katayama S,Minami J,Ohhira I,Okabe A

更新日期：1996-01-01 00:00:00

abstract：:In this review, we have emphasized: 1) bacterial lipopolysaccharide (LPS) involvement in IgA responses to orally administered thymic-dependent (TD) antigens; 2) characterization of Peyer's patch (PP) lymphoreticular cells; and 3) gastrointestinal immunization with gram negative pathogens and anti-LPS immunity to infec...

journal_title：Microbiology and immunology

authors： McGhee JR,Michalek SM,Kiyono H,Eldridge JH,Colwell DE,Williamson SI,Wannemuehler MJ,Jirillo E,Mosteller LM,Spalding DM

更新日期：1984-01-01 00:00:00

abstract：:The effect of fatty acids on Mycobacterium smegmatis was examined in vitro at pH 5.0 to 7.0 to determine the role of fatty acids in the intracellular killing of mycobacteria. Unsaturated fatty acids showed strong bactericidal activity in low concentrations (0.005 to 0.02 mM), whereas saturated fatty acids, except for ...

journal_title：Microbiology and immunology

authors： Kanetsuna F

更新日期：1985-01-01 00:00:00

abstract：:During Helicobacter pylori infection, T cell response is critical in the development of active gastritis and in protective immunity against infection. We studied gastric inflammation and T cell response in H. pylori-challenged mice following an intraperitoneal immunization, using whole H. pylori lysate (HpAg) in the a...

journal_title：Microbiology and immunology

authors： Watanabe K,Murakami K,Maeda K,Fujioka T,Nasu M,Nishizono A

更新日期：2002-01-01 00:00:00

abstract：:The effects of the soluble forms of the endotoxin receptor molecules sMD-2 and sCD14 on bacterial growth were studied. When Escherichia coli and Bacillus subtilis were incubated at 37 degrees C for 18 hr with either sMD-2 or sCD14, growth of these bacteria was significantly inhibited as evaluated by viable cell counts...

journal_title：Microbiology and immunology

authors： Ohnishi T,Muroi M,Tanamoto K

更新日期：2010-02-01 00:00:00

abstract：:Although the BCG vaccine can prevent tuberculosis (TB) in infants, its ability to prevent adult pulmonary TB is reportedly limited. Therefore, development of a novel effective vaccine against pulmonary TB has become an international research priority. We have previously reported that intranasal vaccination of mice wit...

journal_title：Microbiology and immunology

authors： Fukui M,Shinjo K,Umemura M,Shigeno S,Harakuni T,Arakawa T,Matsuzaki G

更新日期：2015-12-01 00:00:00

abstract：:The heat-labile toxin (HLT) of Bordetella bronchiseptica was purified successively from sonic extracts of phase I organisms grown in Stainer-Scholte medium, by partition in hydrophobic interaction, sucrose density gradient centrifugation, gel filtration through Sepharose 4B and 6B, isoelectric precipitation and isoele...

journal_title：Microbiology and immunology

authors： Endoh M,Amitani M,Nakase Y

更新日期：1986-01-01 00:00:00

abstract：:The present study was designed to evaluate the distribution of epithelioid cells, myofibroblasts, and TGF-beta1 in the formation of granuloma caused by Mycobacterium avium intracellulare complex (MAC) lung infection. A retrospective study was performed for 9 cases of positive MAC culture in which lung resections were ...

journal_title：Microbiology and immunology

authors： Fujita J,Ohtsuki Y,Suemitsu I,Yamadori I,Shigeto E,Shiode M,Nishimura K,Hirayama T,Matsushima T,Ishida T

更新日期：2002-01-01 00:00:00

abstract：:A sensitive, quantitative, short-time, and reproducible focus assay for Japanese encephalitis (JE) virus is described. After 2 or 3 days of incubation, the infected cells were treated with anti-JE virus serum and complement, and subsequently stained with trypan blue; then clear foci were produced. This method made it ...

journal_title：Microbiology and immunology

authors： Kimura-Kuroda J,Yasui K

更新日期：1985-01-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) assay for simple detection of Bordetella holmesii was developed. This assay discriminates between B. holmesii and other Bordetella species and successfully detect B. holmesii DNA in nasopharyngeal swab samples from subjects with suspected pertussis. The LAMP assay result...

journal_title：Microbiology and immunology

authors： Otsuka N,Yoshino S,Kawano K,Toyoizumi-Ajisaka H,Shibayama K,Kamachi K

更新日期：2012-07-01 00:00:00

abstract：:Coagulase negative staphylococci (CNS) are significant pathogens, particularly in medical device related infections and in immunocompromised patients. Five CNS strains were isolated from 5 NOS2 knockout mice with dermatitis. Histologically, granulomatous dermatitis was found in the skin around the ear with epidermal u...

journal_title：Microbiology and immunology

authors： Won YS,Kwon HJ,Oh GT,Kim BH,Lee CH,Park YH,Hyun BH,Choi YK

更新日期：2002-01-01 00:00:00

abstract：:To determine the infection source of a sporadic Legionella pneumonia case associated with a hot spring bath, we used five molecular methods, including repetitive element polymerase chain reaction (rep-PCR), arbitrarily primed PCR (AP-PCR), ribotyping, restriction endonuclease analysis (REA), and macrorestriction endon...

journal_title：Microbiology and immunology

authors： Miyamoto H,Jitsurong S,Shiota R,Maruta K,Yoshida S,Yabuuchi E

更新日期：1997-01-01 00:00:00

abstract：:Structural studies were carried out on an O-antigenic polysaccharide moiety derived from Porphyromonas circumdentaria NCTC 12469, a reference strain of Porphyromonas species. The polysaccharide chain was composed of D-glucose, D-galactose, N-acetyl-D-glucosamine, and N-acetyl-D-galactosamine in a molar ratio of 1:2:1:...

journal_title：Microbiology and immunology

authors： Matsuo K,Isogai E,Araki Y

更新日期：2001-01-01 00:00:00

abstract：:Natural killer cells, a critical component of the innate immune system, eradicate both virus-infected cells and tumor cells through cytotoxicity and secretion of cytokines. Human NK cell research has largely been based on in vitro studies because of the lack of appropriate animal models. In this study, a selective pro...

journal_title：Microbiology and immunology

authors： Hattori S,Matsuda K,Kariya R,Harada H,Okada S

更新日期：2016-02-01 00:00:00