Purification and characterization of heat-labile toxin from Bordetella bronchiseptica.

abstract：:Although most influenza vaccines are produced in eggs, new types of vaccines must be developed. In this study, the immunogenicity and safety of a baculovirus-expressed hemagglutinin (HA) of H1N1 influenza virus (Korea/01/2009; designated "HA-Bac-K") was compared with those of a commercially available baculovirus-expre...

journal_title：Microbiology and immunology

authors： Na HN,Kim KH,Song MK,Park HL,Lee EY,Shim SH,Park S,Nam JH

更新日期：2013-09-01 00:00:00

abstract：:Sodium vanadate enhanced Newcastle disease virus (NDV)-induced cell fusion in L cells, and there was a direct correlation between the degree of cell fusion and the dose of vanadate added. When anti-F protein of NDV monospecific antiserum was added to the culture fluid of L cells infected with NDV, the enhancement of c...

journal_title：Microbiology and immunology

authors： Yamamoto F,Fujioka H,Iinuma M,Takano M,Maeno K,Nagai Y,Ito Y

更新日期：1984-01-01 00:00:00

abstract：:ISG20 is an ribonuclease specific for single-stranded RNA and considered to play a role in innate immunity against virus infections. We herein show that both poly IC, an authentic double-stranded RNA, and IFN-gamma induced ISG20 expression in cultured HUVEC. Poly IC-induced ISG20 expression was inhibited by LY294002, ...

journal_title：Microbiology and immunology

authors： Imaizumi T,Mechti N,Matsumiya T,Sakaki H,Kubota K,Yoshida H,Kimura H,Satoh K

更新日期：2008-01-01 00:00:00

abstract：:Periapical lesions are induced by bacterial infection of the dental pulp and result in destruction of the surrounding alveolar bone. Although various immunological studies concerning periapical bone resorption have been reported, the role of cytokines in the formation of periapical lesions remains unclear. In this stu...

journal_title：Microbiology and immunology

authors： Oseko F,Yamamoto T,Akamatsu Y,Kanamura N,Iwakura Y,Imanishi J,Kita M

更新日期：2009-05-01 00:00:00

abstract：:We previously reported a helper T-cell (Th) epitope (peptide i) which corresponded to the sequence ranging from positions 462 to 479 from the N-terminus of the Friend-murine leukemia virus (F-MuLV) envelope protein (env462-479). Homologous sequences exist in both Moloney-murine leukemia (M-MuLV env452-469) and endogen...

journal_title：Microbiology and immunology

authors： Uenishi H,Iwanami N,Yamagishi H,Nakatani T,Kawasaki T,Tamamura H,Fujii N,Kuribayashi K

更新日期：1998-01-01 00:00:00

abstract：:Trehalose 6,6'-dimycolate (TDM), or cord factor, is a crucial stimulus of immune responses during Mycobacterium tuberculosis infection. Although TDM has immuno-stimulatory properties, including adjuvant activity and the ability to induce granuloma formation, the mechanisms underlying these remain unknown. We hypothesi...

journal_title：Microbiology and immunology

authors： Lee WB,Yan JJ,Kang JS,Chung S,Kim LK

更新日期：2017-12-01 00:00:00

abstract：:Cell-mediated immune response to Japanese encephalitis virus (JEV) and its purified envelope (E) protein was measured in 45 laboratory confirmed JE patients using a proliferation assay of peripheral blood mononuclear cells (PBMC). In parallel, JEV-specific IgM antibodies were measured by ELISA. No correlation was obse...

journal_title：Microbiology and immunology

authors： Desai A,Ravi V,Chandramuki A,Gourie-Devi M

更新日期：1995-01-01 00:00:00

abstract：:EAEC is increasingly recognized as an emerging enteric pathogen. Typical EAEC expressing the AggR regulon have been proven to be an important cause of childhood diarrhea in industrialized countries as well as in the developing world, while atypical EAEC without this regulon have not been thoroughly investigated. To in...

journal_title：Microbiology and immunology

authors： Tokuda K,Nishi J,Imuta N,Fujiyama R,Kamenosono A,Manago K,Kawano Y

更新日期：2010-06-01 00:00:00

abstract：:In order to analyze the host-parasite interactions in experimental trichomoniasis, the growth of Trichomonas foetus in the peritoneal cavity and changes in the peritoneal exudate cells were followed in mice treated with dextran sulfate 500 (DS 500), a known macrophage-toxic agent. Light microscopic observation showed ...

journal_title：Microbiology and immunology

authors： Oka Y,Hayashi H,Ishikawa F,Osaki H

更新日期：1980-01-01 00:00:00

abstract：:Mg2+-availability in Staphylococcus aureus cells decreased significantly with increasing NaCl concentration in growth media. Cells grew in a NaCl-free, Chelex resin-treated complex medium only if the medium was supplemented with 50 microM MgCl2, while, growth was limited when the medium was further supplemented with 1...

journal_title：Microbiology and immunology

authors： Ochiai T

更新日期：2001-01-01 00:00:00

abstract：:Biofilm formation is an important phenotype associated with chronic Pseudomonas aeruginosa infections. In the present study, a total of 48 P. aeruginosa strains isolated from clinical specimens were examined for their biofilm-forming ability using a microtiter plate method. The different biofilm-forming abilities were...

journal_title：Microbiology and immunology

authors： Yang W,Shi L,Jia WX,Yin X,Su JY,Kou Y,Yi X,Shinoda S,Miyoshi S

更新日期：2005-01-01 00:00:00

abstract：:Water-insoluble glucan (WIG) produced by mutans streptococci, an important cariogenic pathogen, plays an important role in the formation of dental biofilm and adhesion of biofilm to tooth surfaces. Glucanohydrolases, such as mutanase (α-1,3-glucanase) and dextranase (α-1,6-glucanase), are able to hydrolyze WIG. The pu...

journal_title：Microbiology and immunology

authors： Otsuka R,Imai S,Murata T,Nomura Y,Okamoto M,Tsumori H,Kakuta E,Hanada N,Momoi Y

更新日期：2015-01-01 00:00:00

abstract：:β-1,3-glucan is a major component of fungal cell walls with various biological activities, including effects on the production of inflammatory mediators in vivo and in vitro. However, few reports have examined its influence on the fungal cell itself. In this study, the influences of β-1,3-glucan on the growth and cell...

journal_title：Microbiology and immunology

authors： Ishibashi K,Kurone Y,Motoi M,Miura NN,Adachi Y,Shirasu Y,Ohno N

更新日期：2010-11-01 00:00:00

abstract：:RobA is a member of the XylS/AraC subfamily of DNA binding proteins, and when overexpressed, it induces multiple antibiotic resistance in Escherichia coli. In this study, we introduced a multicopy robA plasmid (pMEP1) and its derivative into OmpF mutants and an AcrAB-deficient mutant. We found that a decrease in susce...

journal_title：Microbiology and immunology

authors： Tanaka T,Horii T,Shibayama K,Sato K,Ohsuka S,Arakawa Y,Yamaki K,Takagi K,Ohta M

更新日期：1997-01-01 00:00:00

abstract：:The nuclear factor-kappa B (NF-kappaB) plays a critical role in regulating expression of genes responsible for a wide range of cellular processes, including innate and adoptive immune responses, and pathways related to cell survival and proliferation. Based on its property as a transcription factor, NF-kappaB has been...

journal_title：Microbiology and immunology

authors： Isomura I,Morita A

更新日期：2006-01-01 00:00:00

abstract：:During the three-year period from 1984 to 1987, 506 acute gastroenteritis outbreaks involving 14,383 patients were reported to the Bureau of Public Health, Tokyo Metropolitan Government. Eighty (4,324 patients) of 150 outbreaks (4,860 patients) from which etiologic agents were not identified were subjected to virologi...

journal_title：Microbiology and immunology

authors： Sekine S,Okada S,Hayashi Y,Ando T,Terayama T,Yabuuchi K,Miki T,Ohashi M

更新日期：1989-01-01 00:00:00

abstract：:B lymphocytes express multiple TLRs that regulate their cytokine production.We investigated the effect of TLR4 and TLR9 activation on receptor activator of NF‐kB ligand (RANKL) expression by rat spleen B cells. Splenocytes or purified spleen B cells from Rowett rats were cultured with TLR4 ligand Escherichia coli LPS ...

journal_title：Microbiology and immunology

authors： Yu X,Lin J,Yu Q,Kawai T,Taubman MA,Han X

更新日期：2014-01-01 00:00:00

abstract：:Scanning electron micrograph of HeLa S3 monolayered cells, inoculated with viable bacteria of a Salmonella typhi strain GIFU 10007, revealed that the extended microvilli tangled the bacteria within 10 min after inoculation. The micrographs of HeLa cells, at 1 hr after inoculation, indicate the following: shortening of...

journal_title：Microbiology and immunology

authors： Yokoyama H,Ikedo M,Kohbata S,Ezaki T,Yabuuchi E

更新日期：1987-01-01 00:00:00

abstract：:Opaque (Op) and translucent (Tr) colonial variants were isolated from Vibrio vulnificus strains. Op-type variants were more resistant than the isogenic Tr-type variants, but the survival rate of the Op-type variants varied with the strains. Antisera were prepared by immunizing rabbit with whole cells of Op and Tr vari...

journal_title：Microbiology and immunology

authors： Shinoda S,Kobayashi M,Yamada H,Yoshida S,Ogawa M,Mizuguchi Y

更新日期：1987-01-01 00:00:00

abstract：:The immunostimulatory effect of intragastrically or parenterally administered beta-(1-->3; 1-->4) glucan, extracted from oats (ObetaG), on disease resistance to Eimeria vermiformis was studied in C57BL/6 mice. Multiple administrations of ObetaG by intragastric or subcutaneous routes reduced fecal oocyst shedding compa...

journal_title：Microbiology and immunology

authors： Yun CH,Estrada A,Van Kessel A,Gajadhar A,Redmond M,Laarveld B

更新日期：1998-01-01 00:00:00

abstract：:Stromal cell-derived factor-1 (SDF-1) is an efficacious chemoattractant for lymphocytes, monocytes and hematopoietic progenitor cells. In the present study, we examined whether SDF-1 has growth promoting activity on human peripheral T cells and analyzed the possible underlying signal transduction pathways. SDF-1 augme...

journal_title：Microbiology and immunology

authors： Yonezawa A,Hori T,Sakaida H,Uchiyama T

更新日期：2000-01-01 00:00:00

abstract：:A polymyxin B (PXB)-resistant mutant of Klebsiella pneumoniae O3 was isolated. Lipopolysaccharide (LPS) extracted from the PXB-resistant isolate bound little PXB, although LPS from the parental strain did. The 31P nuclear magnetic resonance (NMR) spectrum of PXB-resistant type LPS showed that it contained much less of...

journal_title：Microbiology and immunology

authors： Yokochi T,Inoue Y,Jiang GZ,Kato Y,Sugiyama T,Kawai M,Fukada M,Takahashi K

更新日期：1994-01-01 00:00:00

abstract：:Francisella tularensis, which causes tularemia, is widely distributed in the Northern hemisphere. F. tularensis strains isolated in Japan are genetically unique from non-Japanese strains; however, their phenotypic properties have not been well studied. Thus, mice were infected with representative Japanese strains of F...

journal_title：Microbiology and immunology

authors： Hotta A,Fujita O,Uda A,Yamamoto Y,Sharma N,Tanabayashi K,Yamada A,Morikawa S

更新日期：2016-03-01 00:00:00

abstract：:The mechanism of MHC-restricted T and B cell interactions in antibody response was studied with IgM-inducible B hybridomas and antigen-specific helper T cell clones. B hybridomas were prepared by fusion between splenic B cells from (CBA/N (H-2k) X BALB/c (H-2d)) F1 (NBF1) male mice and a B lymphoma cell line, M12.4.5....

journal_title：Microbiology and immunology

authors： Yoshikubo T,Kimoto M,Kishimoto T

更新日期：1985-01-01 00:00:00

abstract：:Sixteen temperature-sensitive mutants of Sendai virus were isolated from mutagenized stocks (10 mutants, designated numerically) and persistently infected cultures (6 mutants, designated alphabetically). Based on complementation tests, virion-associated activities, thermal inactivation, and viral RNA and hemadsorbing ...

journal_title：Microbiology and immunology

authors： Adachi A,Kanda T,Shibuta H

更新日期：1980-01-01 00:00:00

abstract：:The standard products of V(D)J recombination of immunoglobulin and T cell receptor genes are two kinds of DNA junction, a coding joint and a signal joint. TCR delta V-D and D-D signal joints in adult mouse thymocytes were sequenced following PCR amplification. We observed differential nucleotide insertions at the V de...

journal_title：Microbiology and immunology

authors： Kanari Y,Muto M,Yamagishi H

更新日期：2003-01-01 00:00:00

abstract：:Natural killer cells, a critical component of the innate immune system, eradicate both virus-infected cells and tumor cells through cytotoxicity and secretion of cytokines. Human NK cell research has largely been based on in vitro studies because of the lack of appropriate animal models. In this study, a selective pro...

journal_title：Microbiology and immunology

authors： Hattori S,Matsuda K,Kariya R,Harada H,Okada S

更新日期：2016-02-01 00:00:00

abstract：:An antiserum against a fraction enriched for scrapie-associated fibrils (SAF), was examined for its effects on scrapie incubation period by inoculating mice either intraperitoneally or intracerebrally with various dilutions of the serum mixed with scrapie-infected mouse brain homogenate. After intraperitoneal inoculat...

journal_title：Microbiology and immunology

authors： Ito M,Shinagawa M,Doi S,Sasaki S,Isomura H,Takahashi K,Goto H,Sato G

更新日期：1988-01-01 00:00:00

abstract：:Susceptibility of eight strains of influenza A and B viruses to interferon and to poly(I) . poly(C) were determined by the plaque reduction method. All strains tested were slightly less susceptible than vesicular stomatitis virus (VSV) in an established line of canine kidney (MDCK) cells. The 50% plaque depression dos...

journal_title：Microbiology and immunology

authors： Machida H,Kuninaka A,Yoshino H

更新日期：1980-01-01 00:00:00

abstract：:Intestinal intraepithelial T cells (IELs) expressing either gammadelta TCR or alphabeta TCR have been proposed to play an important role in the regulation of intestinal epithelia by producing cytokines that directly influence the adjoining intestinal epithelial cell (IEC) functions. To illuminate this issue, we utiliz...

journal_title：Microbiology and immunology

authors： Kohyama M,Hachimura S,Nanno M,Ishikawa H,Kaminogawa S

更新日期：1997-01-01 00:00:00