RobA-induced multiple antibiotic resistance largely depends on the activation of the AcrAB efflux.

Abstract:

:RobA is a member of the XylS/AraC subfamily of DNA binding proteins, and when overexpressed, it induces multiple antibiotic resistance in Escherichia coli. In this study, we introduced a multicopy robA plasmid (pMEP1) and its derivative into OmpF mutants and an AcrAB-deficient mutant. We found that a decrease in susceptibility to multiple antibiotics in these OmpF mutants when pMEP1 was introduced did not depend on OmpF porin expression. Interestingly, a delta ompF mutant (TK007) became more sensitive when pMEP1 was introduced. Moreover, no effect of RobA on the induction of multiple antibiotic resistance in an acrA1- mutant was observed. Therefore, we conclude that the multiple antibiotic resistance induced by the overexpression of RobA largely depends on the activation of the AcrAB efflux, as well as the activation of micF.

journal_name

Microbiol Immunol

authors

Tanaka T,Horii T,Shibayama K,Sato K,Ohsuka S,Arakawa Y,Yamaki K,Takagi K,Ohta M

doi

10.1111/j.1348-0421.1997.tb01913.x

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

697-702

issue

9

eissn

0385-5600

issn

1348-0421

journal_volume

41

pub_type

杂志文章
  • An indirect microhemagglutination test for Lyme disease.

    abstract::We have developed an indirect microhemagglutination test (IMHA) for detecting antibodies to Borrelia burgdorferi using formalin-fixed chicken erythrocytes sensitized with a sonicated extract of the bacteria. The specificity of IMHA for Lyme disease was compared with that of a conventional indirect immunoperoxidase tec...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1991.tb01590.x

    authors: Fujita H,Sato T,Ohara Y,Homma M

    更新日期:1991-01-01 00:00:00

  • Identification of heat-stable enterotoxin-producing strains of Yersinia enterocolitica and Vibrio cholerae non-O1 by a monoclonal antibody-based enzyme-linked immunosorbent assay.

    abstract::Using a mouse monoclonal antibody (MAb) 2F raised against Vibrio cholerae non-O1 heat-stable enterotoxin (NAG-ST) which also recognizes a shared epitope of Yersinia enterocolitica heat-stable enterotoxin (Y-ST), a competitive enzyme-linked immunosorbent assay (ELISA) was developed for independent detection of NAG-ST a...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1993.tb03198.x

    authors: Nair GB,Bhattacharya SK,Takeda T

    更新日期:1993-01-01 00:00:00

  • The kinetics of urinary shedding of BK virus in children with renal disease.

    abstract::Children with renal diseases are typically treated with immunosuppressive drugs, which place them at high risk of reactivation of the BK virus (BKV). Currently, little is known about the impact of immunosuppressive drugs on the kinetics of urinary shedding of BKV and viral reactivation in pediatric patients with renal...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12212

    authors: Yamamoto Y,Morooka M,Ihira M,Yoshikawa T

    更新日期:2015-01-01 00:00:00

  • Induction of protective immunity by primed B-1 cells in Toxoplasma gondii -infected B cell-deficient mice.

    abstract::We examined the role of B-1 cells in protection against Toxoplasma gondii infection using B cell-deficient mice (muMT mice). We found that primed but not naïve B-1 cells from wild-type C57BL/6 mice protected B cell-deficient recipients from challenge infection. All muMT mice transferred with primed B-1 cells survived ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2003.tb03460.x

    authors: Chen M,Mun HS,Piao LX,Aosai F,Norose K,Mohamed RM,Belal US,Fang H,Ahmed AK,Kang HK,Matsuzaki G,Kitamura D,Yano A

    更新日期:2003-01-01 00:00:00

  • Protective effects of in vivo-expressed autotransporters against Bordetella pertussis infection.

    abstract::Bordetella pertussis causes whooping cough, a severe and prolonged respiratory disease that results inhas high morbidity and mortality rates, particularly in developing countries. The number incidence of whooping cough cases is increasing in many countries despite high vaccine coverage. Causes for the re-emergence of ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12504

    authors: Suzuki K,Shinzawa N,Ishigaki K,Nakamura K,Abe H,Fukui-Miyazaki A,Ikuta K,Horiguchi Y

    更新日期:2017-09-01 00:00:00

  • Diagnostic value of serum concentrations of high-mobility group-box protein 1 and soluble hemoglobin scavenger receptor in brucellosis.

    abstract::Both cluster of differentiation (CD)4(+) and CD8(+) T lymphocytes play key roles in immunity to Brucella, in part because they secrete interferon (IFN)-γ and activate bactericidal functions in macrophages. Therefore, use of markers of macrophage activation may have diagnostic and prognostic significance. High-mobili...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12016

    authors: Ayarcı AO,Yılmaz E,Sığırlı D,Budak F,Göral G,Oral HB

    更新日期:2013-02-01 00:00:00

  • Genetic control of immune response to a synthetic fimbrial antigen of Actinobacillus actinomycetemcomitans.

    abstract::The incidence of infection by Actinobacillus actinomycetemcomitans, one of the important pathogens in human periodontal diseases, has been reported to be associated with racial background and genetic factors. We attempted to determine the genetic regulation of immune responses to A. actinomycetemcomitans fimbriae, an ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1997.tb01899.x

    authors: Honma K,Ishii N,Kato T,Ishihara K,Okuda K,Okuda K

    更新日期:1997-01-01 00:00:00

  • The transposon-like structure of IS26-tetracycline, and kanamycin resistance determinant derived from transferable R plasmid of fish pathogen, Pasteurella piscicida.

    abstract::Tetracycline (pp-tet), and kanamycin (pp-kan) resistance genes were cloned from a transferable R plasmid of fish pathogen Pasteurella piscicida, and complete nucleotide sequences were determined. The pp-tet was a class D Tet determinant constructed with the tetA resistance gene of 1,182 bp encoding a protein with a de...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1994.tb01741.x

    authors: Kim EH,Aoki T

    更新日期:1994-01-01 00:00:00

  • In situ infiltration of natural killer-like cells induced by intradermal injection of the nucleic acid fraction from BCG.

    abstract::Intradermal injection of MY-1, a nucleic acid fraction extracted from Mycobacterium bovis strain BCG, induced in situ infiltration of mononuclear cells, most of which were asialo GM1 (GA1)-positive as determined by immunofluorescence microscopy. The infiltration occurred with as little as 1 microgram of MY-1 and laste...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1989.tb00980.x

    authors: Kuramoto E,Toizumi S,Shimada S,Tokunaga T

    更新日期:1989-01-01 00:00:00

  • Pharmacological effect of beta toxin of Clostridium perfringens type C on rats.

    abstract::The biological effect of purified beta toxin of Clostridium perfringens type C in vivo was investigated. After intravenous injection of the purified beta toxin into rats, a rise in blood pressure and a simultaneous fall in heart rate were observed. After the blood pressure reached a maximum, the heart rate recovered g...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1981.tb00045.x

    authors: Sakurai J,Fujii Y,Matsuura M,Endo K

    更新日期:1981-01-01 00:00:00

  • Immunopotentiating activity of whole cells and mini-cells of Salmonella typhimurium.

    abstract::Salmonella typhimurium strain 9 produces mini-cells during cell proliferation. Mini-cells are viable but cannot proliferate since they do not contain chromosomal DNA. Effects of whole cells and mini-cells of S. typhimurium on the immune responses were investigated, with the following results. Phagocytosis of peritonea...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1981.tb00049.x

    authors: Kurashige S,Yoshida T,Kodama K,Mitsuhashi S

    更新日期:1981-01-01 00:00:00

  • Linkage of ribosomal RNA genes in Leptospira.

    abstract::We determined the linkage of 16S, 23S, and 5S rRNA genes in several strains of Leptospira and Leptonema by DNA-DNA hybridization. Almost all the hybridizations in all leptospires used in these experiments gave two radioactive bands and the results strongly suggest that the number of the 16S and the 23S rRNA genes in t...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1990.tb01033.x

    authors: Fukunaga M,Masuzawa T,Okuzako N,Mifuchi I,Yanagihara Y

    更新日期:1990-01-01 00:00:00

  • Endogenous inhibitors of factor B.

    abstract::proteins which were able to bind noncovalently with mouse factor B were found in cells that are nonsecretors of factor B such as mouse-established monocytic cells and L cells but not in peritoneal resident macrophages. These proteins were isolated from lysates of L cells and separated into four distinct proteins by pr...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1983.tb02941.x

    authors: Moriyama T,Kawamoto Y,Ichikawa H,Okamoto K,Inoue T,Kobayashi T,Miyama A

    更新日期:1983-01-01 00:00:00

  • Engineering large viral DNA genomes using the CRISPR-Cas9 system.

    abstract::Manipulation of viral genomes is essential for studying viral gene function and utilizing viruses for therapy. Several techniques for viral genome engineering have been developed. Homologous recombination in virus-infected cells has traditionally been used to edit viral genomes; however, the frequency of the expected ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12180

    authors: Suenaga T,Kohyama M,Hirayasu K,Arase H

    更新日期:2014-09-01 00:00:00

  • Protective immunity against Bordetella pertussis by a recombinant DNA vaccine and the effect of coinjection with a granulocyte-macrophage colony stimulating factor gene.

    abstract::A recombinant pertussis DNA vaccine was described here with its immunogenicity and the ability to induce protection against B. pertussis infection in mice. Three immunodominant antigen gene fragments of pertussis, pertussis toxin subunit 1 (pts1), fragments of pertactin (prn) and filamentous hemagglutinin (fha), were ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2006.tb03869.x

    authors: Li Q,Zhu Y,Chu J,Wang Y,Xu Y,Hou Q,Zhang S,Guo X

    更新日期:2006-01-01 00:00:00

  • Inhibitory role of neutrophils on influenza virus multiplication in the lungs of mice.

    abstract::The protective role of neutrophils on intranasal infection of influenza virus was investigated in 3 strains of tumor-bearing mice with neutrophilic leukocytosis. In vitro multiplication of influenza virus was inhibited by neutrophils from both normal and tumor-bearing mice, and the inhibitory effect of neutrophils was...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2001.tb01302.x

    authors: Fujisawa H

    更新日期:2001-01-01 00:00:00

  • Mechanisms of action of corilagin and tellimagrandin I that remarkably potentiate the activity of beta-lactams against methicillin-resistant Staphylococcus aureus.

    abstract::Corilagin and tellimagrandin I are polyphenols isolated from the extract of Arctostaphylos uvaursi and Rosa canina L. (rose red), respectively. We have reported that corilagin and tellimagrandin I remarkably reduced the minimum inhibitory concentration (MIC) of beta-lactams in methicillin-resistant Staphylococcus aure...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2004.tb03489.x

    authors: Shiota S,Shimizu M,Sugiyama J,Morita Y,Mizushima T,Tsuchiya T

    更新日期:2004-01-01 00:00:00

  • Attachment of Mycoplasma pulmonis to rat and mouse synovial cells cultured in vitro.

    abstract::The attachment of Mycoplasma pulmonis m53 organisms to mouse and rat synovial cells was examined by using the organisms and the synovial cells treated in various ways. M. pulmonis treated with trypsin attached to the synovial cells, but the organisms treated with pronase, formaldehyde, glutaraldehyde, or heat did not....

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1985.tb00864.x

    authors: Araake M,Yayoshi M,Yoshioka M

    更新日期:1985-01-01 00:00:00

  • Leukocyte integrin-dependent and antibody-independent cytotoxicity of macrophage against allografts.

    abstract::Macrophages (Mphis), but not T cells, infiltrating into the rejection site of either i.p. allografted Meth A (H-2d) fibrosarcoma cells in C57BL/6 (B6) (H-2b) mice or BALB/c (H-2d) skin onto B6 mice are cytotoxic against allografts with H-2d specificity. To determine the mechanisms of specific killing of allografts by ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2000.tb01246.x

    authors: Yoshida R,Oku T,Takikawa O,Einaga-Naito K,Yoneda Y,Hirota R,Kubota T

    更新日期:2000-01-01 00:00:00

  • Physical and functional map of an amikacin-resistance plasmid isolated from a multiresistant strain of Serratia marcescens.

    abstract::pTK159, a multiresistance 40-kilobase (kb) plasmid, was isolated from a clinical strain of Serratia marcescens. pTK159 was nonconjugative and carried determinants for resistance to amikacin, streptomycin/spectinomycin, sulfamethoxazole and ampicillin. A physical and functional map of pTK159 was constructed. By cloning...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1988.tb01384.x

    authors: Kanno R

    更新日期:1988-01-01 00:00:00

  • Cluster analysis of human and animal pathogenic Microsporum species and their teleomorphic states, Arthroderma species, based on the DNA sequences of nuclear ribosomal internal transcribed spacer 1.

    abstract::We performed a cluster analysis of human and animal pathogenic Microsporum species and their teleomorphic states, Arthroderma species, including A. otae-related species (M. canis, M. audouinii, M. distortum, M. equinum, M. langeronii, and M. ferrugineum) and M. gypseum complex (A. fulvum, A. gypseum, and A. incurvatum...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2001.tb02609.x

    authors: Makimura K,Tamura Y,Murakami A,Kano R,Nakamura Y,Hasegawa A,Uchida K,Yamaguchi H

    更新日期:2001-01-01 00:00:00

  • A role for lipid rafts in immune cell signaling.

    abstract::Cross-linking of surface receptors in hematopoietic cells results in the enrichment of these receptors in the rafts along with other downstream signaling molecules. A possible explanation how signal is transduced through the plasma membrane has arisen from the concept of raft. From the study of cellular responses in t...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1348-0421.2001.tb01259.x

    authors: Katagiri YU,Kiyokawa N,Fujimoto J

    更新日期:2001-01-01 00:00:00

  • Gene expression profiling of polymorphonuclear leukocytes treated with the culture filtrate of Aspergillus fumigatus and gliotoxin.

    abstract::Pathogens of the Aspergillus species are frequently seen in deep-seated mycoses. We previously demonstrated that the culture filtrate of Aspergillus fumigatus (CF) has immunosuppressive effects on polymorphonuclear leukocytes (PMNs), which act as the main phagocytes to hyphae of Aspergillus fumigatus (A. fumigatus). B...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2007.tb03928.x

    authors: Higurashi H,Arai M,Watanabe A,Igari H,Seki N,Kamei K,Kuriyama T

    更新日期:2007-01-01 00:00:00

  • Apoptosis observed in murine peritoneal macrophages treated with interferon gamma through staphylococcal enterotoxin-dependent cell-mediated cytotoxicity.

    abstract::The concept of superantigens is well-known and widely accepted. In this brief communication, we analyze the behaviour of antigen-presenting cells after T-cell activation by staphylococcal enterotoxin B, a representative superantigen. We tried to activate murine T cells by inflammatory mouse peritoneal macrophage in th...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2000.tb02603.x

    authors: Sakurada J,Tajima A,Shinji H,Seki K,Masuda S

    更新日期:2000-01-01 00:00:00

  • Spodiobacter cordis gen. nov. sp. nov., a member of the family Flavobacteriaceae isolated from patients with infective endocarditis.

    abstract::Two gram-negative, catalase-negative, oxidase-positive strains (PAGU 1467T and PAGU 1468) isolated from patients with infective endocarditis were investigated to determine their taxonomic status. 16S rRNA gene sequence analysis indicated that the two strains were members of the Bergeyella-Chryseobacterium-Riemerella b...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/1348-0421.12673

    authors: Tomida J,Fujiwara N,Naka T,Morita Y,Sawabe E,Tojo N,Kikuchi K,Kawamura Y

    更新日期:2019-03-01 00:00:00

  • Suppression of phorbol myristate acetate-triggering of macrophage H2O2 release by sarcoma 180 originating factor.

    abstract::A high molecular weight proteinaceous factor in the cell extract of sarcoma 180 (S-180) was found to inhibit phorbol myristate acetate (PMA)-triggering of macrophage H2O2 release. This factor (S-180 factor) was stable at 56 C for 1 hr and resistant to ultraviolet-irradiation. The S-180 factor inhibited the specific bi...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1985.tb00900.x

    authors: Tomioka H,Saito H

    更新日期:1985-01-01 00:00:00

  • Nature of adult T-cell leukemia-associated membrane antigen on the surface of adult T-cell leukemia virus-carrying cells as revealed by membrane immunofluorescence.

    abstract::Adult T-cell leukemia-associated membrane antigen (ATLMA) expressed on the surface of living ATL virus (ATLV)-carrying cells was investigated by an indirect membrane immunofluorescence method using natural antibodies to ATLV in human sera. All the ATLV-positive cell lines tested that had cytoplasmic ATL-associated ant...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1984.tb00696.x

    authors: Chosa T,Yamamoto N,Koyanagi Y,Kohno M,Nagata K,Hinuma Y

    更新日期:1984-01-01 00:00:00

  • Menadione-catalyzed O2- production by Escherichia coli cells: application of rapid chemiluminescent assay to antimicrobial susceptibility testing.

    abstract::This study proposes a novel chemiluminescent assay of bacterial activity. Luminol chemiluminescence (LC) was amplified on addition of menadione to Escherichia coli suspension, and it was effectively inhibited by addition of superoxide dismutase rather than catalase. This fact suggests that H2O2 produced from O2 by sup...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2001.tb02628.x

    authors: Yamashoji S,Manome I,Ikedo M

    更新日期:2001-01-01 00:00:00

  • Clostridium botulinum type C in healthy swine in Japan.

    abstract::Healthy cattle and swine bred in a district of Japan were examined for the presence of Clostridium botulinum in their liver. Liver specimens were cultivated in chopped meat-glucose medium and the cultures were examined for botulinum toxin. In cattle, none of the cultures of 100 liver specimens yielded the toxin. In sw...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.1992.tb01639.x

    authors: Yamakawa K,Kamiya S,Yoshimura K,Nakamura S

    更新日期:1992-01-01 00:00:00

  • Screening method for Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones by PCR-restriction fragment length polymorphism.

    abstract::Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones (MICs of ciprofloxacin, 0.25 to 2 microg/ml) have a mutation at codon either Ser-83 or Asp-87 of gyrA gene. A screening method by PCR-restriction fragment length polymorphism (PCR-RFLP) was designed to screen the ...

    journal_title:Microbiology and immunology

    pub_type: 杂志文章

    doi:10.1111/j.1348-0421.2003.tb02800.x

    authors: Hirose K,Tamura K,Watanabe H

    更新日期:2003-01-01 00:00:00