Abstract:
:We determined the linkage of 16S, 23S, and 5S rRNA genes in several strains of Leptospira and Leptonema by DNA-DNA hybridization. Almost all the hybridizations in all leptospires used in these experiments gave two radioactive bands and the results strongly suggest that the number of the 16S and the 23S rRNA genes in those strains is two, respectively. In contrast with the larger rRNAs, the number of 5S rRNA gene was different. In the strains of leptospires, L. biflexa, which were non-parasitic, there are two genes for 5S rRNA, whereas only one gene for 5S rRNA is carried in L. interrogans, which were originally isolated as parasitic. Southern hybridization experiments suggest that those rRNA genes are interspersed on the leptospiral chromosome.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Fukunaga M,Masuzawa T,Okuzako N,Mifuchi I,Yanagihara Ydoi
10.1111/j.1348-0421.1990.tb01033.xsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
565-73issue
7eissn
0385-5600issn
1348-0421journal_volume
34pub_type
杂志文章abstract::Five groups of 32 chemicals were examined regarding their immunological functions as modifier inactivants to make inactivated Sendai nasal vaccine using a contact exposure experiment, direct immunofluorescent method, and serum HI titer. (1) Five of the nine reactive groups of reactive dyes (2-chloropyridine, 2, 4, 6-t...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1996.tb01138.x
更新日期:1996-01-01 00:00:00
abstract::Chronic hepatitis C virus (HCV) infection affects approximately 170 million people worldwide. HCV infection is a major global health problem as it can be complicated with liver cirrhosis and hepatocellular carcinoma. So far, there is no vaccine available and the non-specific, interferon (IFN)-based treatments now in u...
journal_title:Microbiology and immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1348-0421.2011.00403.x
更新日期:2012-01-01 00:00:00
abstract::There is a deficiency in the data concerning the role of hMPV in lower respiratory tract infections in adults, and until now there has been no data available regarding the prevalence of hMPV in adults in our region. In the present study the association of hMPV with varieties of lower respiratory tract disorders in imm...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2009.00162.x
更新日期:2009-11-01 00:00:00
abstract::Twelve monoclonal antibodies against Negishi virus were obtained and characterized by hemagglutination inhibition (HI) and neutralization (NT) tests using five flaviviruses isolated in the pan-Pacific region. The reaction pattern of the antibodies showed that Negishi virus was most closely related to Langat virus, fol...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1984.tb00758.x
更新日期:1984-01-01 00:00:00
abstract::The effects of added maltose on the activities of a preparation of crude glucosyltransferases (GTases) and purified dextransucrase (DS) were investigated to elucidate the inhibition mechanism of maltose on the synthesis of water-insoluble glucan (ISG) in Streptococcus mutans HS-6. Tri- and tetra-saccharides produced b...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1983.tb00657.x
更新日期:1983-01-01 00:00:00
abstract::The use of continuous free-flow electrophoresis for the purification of extracted lipopolysaccharides ( LPSs ) was investigated. Commercial (nucleic acid contaminated) LPS preparations, isolated by the hot phenol-water method of Westphal from Salmonella typhimurium and Escherichia coli 0111: B4, were analyzed. Continu...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1984.tb00668.x
更新日期:1984-01-01 00:00:00
abstract::Nontypeable Haemophilus influenzae (NTHi), an important human respiratory pathogen, frequently causes biofilm infections. Currently, resistance of bacteria within the biofilm to conventional antimicrobials poses a major obstacle to effective medical treatment on a global scale. Novel agents that are effective against ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12194
更新日期:2014-10-01 00:00:00
abstract::Bifidobacteria are important members of the intestinal microbiota and are considered to contribute to maintaining health. However, the level of bifidobacteria colonising the intestine of elderly subjects tends to be lower than in younger adults. Therefore, two Bifidobacterium longum strains, isolated from healthy elde...
journal_title:Microbiology and immunology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1348-0421.2003.tb03464.x
更新日期:2003-01-01 00:00:00
abstract::A solubilized sheep red blood cell (SRBC) antigen (supernatant fraction obtained by centrifuging 10(7)-2 x 108 sonicated SRBC at 6 x 10(4) g for 30 min [Sup-SRBC]), whose ability to inhibit anti-SRBC plaque formation was 70% of that of the original sonicated SRBC, was unable to elicit a detectable antibody response in...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1982.tb00163.x
更新日期:1982-01-01 00:00:00
abstract::A 4-year (1996-2000) survey of rotavirus infection involving 2,218 diarrheal fecal specimens of children collected from five regions of Japan was conducted. A total of 642 (28.9%) specimens were found to be rotavirus positive. A changed prevalence pattern of rotavirus G serotype was found with an increase of G9 and G2...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2003.tb03422.x
更新日期:2003-01-01 00:00:00
abstract::Cattle enterotoxemia caused by Clostridium perfringens toxins is a noncontagious, sporadic, and fatal disease characterized by sudden death. Strategies for controlling and preventing cattle enterotoxemia are based on systematic vaccination of herds with toxoids. Because the process of producing conventional clostridia...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12198
更新日期:2014-11-01 00:00:00
abstract::Recombinant human interferon-gammas (rHuIFN-gamma s) were obtained from two different mammalian cells (mouse C127 cells and Chinese hamster ovary, CHO, cells) cultured in a microcarrier culture system. Both rHuIFN-gamma s were purified using sequential chromatographies for their comparison of structural properties. Th...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1988.tb01410.x
更新日期:1988-01-01 00:00:00
abstract::Four N-acetylchito-oligosaccharides, from tetra-N-acetylchitotetraose (NACOS-4) to hepta-N-acetylchitoheptaose (NACOS-7), were found to increase the number of peritoneal exudate cells (PEC) in male BALB/c mice after 3 hr intraperitoneal administration of 50 mg/kg of each oligosaccharide. The number of attracted cells,...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1986.tb03004.x
更新日期:1986-01-01 00:00:00
abstract::Staphylococcal exfoliative toxin A (ETA) had a splitting effect at the granular layer of skin in humans and neonatal mice, but not in rabbits, guinea pigs, golden hamsters, or rats. Besides its splitting effect, ETA could stimulate productions of neutralizing antibody to ETA in rabbits, rats and B10D2 mice, but not in...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1988.tb01472.x
更新日期:1988-01-01 00:00:00
abstract::Vibrio parahaemolyticus mutants lacking three Na+/H+ antiporters (NhaA, NhaB, NhaD) were constructed. The DeltanhaA strains showed significantly higher sensitivity to LiCl regarding their growth compared to the parental strain. The DeltanhaA and DeltanhaB strains exhibited higher sensitivities to LiCl. The mutant XACa...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2005.tb03662.x
更新日期:2005-01-01 00:00:00
abstract::Antibodies to influenza A virus were detected using enzyme-linked immunosorbent assay (ELISA) in the sera from two of seven Baikal seals (Phoca sibrica) and from five of six ringed seals (Phoca hispida) in Russia. In a hemagglutination-inhibition test using H1-H15 reference influenza A viruses, ELISA-positive sera fro...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2004.tb03610.x
更新日期:2004-01-01 00:00:00
abstract::Erythematous delayed reactions without induration, presumably assigned to Jones-Mote type, were characterized by the resistance to treatment with cyclophosphamide (CY) before elicitation or immunization in guinea pigs immunized with BGG in IFA or CFA. CY-treatment before elicitation converted delayed erythematous reac...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1978.tb00425.x
更新日期:1978-01-01 00:00:00
abstract::Escherichia coli O157 strains starved in sterile deionized water (SDW) and filter-sterilized natural river water (SRW) were investigated with specific reference to their culturability in selective and non-selective media. Growth of the strains starved in both SDW and SRW were markedly suppressed with time in selective...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1999.tb02396.x
更新日期:1999-01-01 00:00:00
abstract::Noroviruses cause acute gastroenteritis. Since multiple genotypes of norovirus co-circulate in humans, changing the genotype composition and eluding host immunity, development of a polyvalent vaccine against norovirus in which the genotypes of vaccine strains match the major strains in circulation in the target season...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12384
更新日期:2016-06-01 00:00:00
abstract::A human T-lymphoblastoid cell line, TCL-Fuj, produces large amounts of interferon (IFN)-gamma constitutively. A variant cell line, 2M, was derived from it. Both cell lines express similar surface antigen markers, but differ in surface morphology. Compared with the parent TCL-Fuj cell line, 2M produced less IFN-gamma c...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1987.tb03116.x
更新日期:1987-01-01 00:00:00
abstract::There are two types of carboxypeptidases present in human blood, carboxypeptidase N (CPN) and arginine carboxypeptidase (CPR). CPR is generated during coagulation from a precursor (proCPR) which can be converted to the active form by trypsin in vitro. Since it is difficult to distinguish the two types of carboxypeptid...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1999.tb02457.x
更新日期:1999-01-01 00:00:00
abstract::Alkaline phosphatase-conjugated oligonucleotide probes were developed to detect the gene coding for Vero toxin 1 (VT1) and Vero toxin 2 (VT2). Using these probes, 3 hr was enough to detect VT genes when suspicious colonies of enterohaemorrhagic Escherichia coli (EHEC) were obtained on an agar plate. The results of a h...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1997.tb01944.x
更新日期:1997-01-01 00:00:00
abstract::An oral delivery system based on ApxIIA#5-expressed on Saccharomyces cerevisiae was studied for its potential to induce immune responses in mice. Murine bone marrow-derived dendritic cells (DCs) stimulated in vitro with ApxIIA#5-expressed on S. cerevisiae upregulated the expression of maturation and activation markers...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/1348-0421.12053
更新日期:2013-06-01 00:00:00
abstract::Diarrheal diseases are major causes of morbidity and mortality among children in developing countries. We have analyzed the causative agents of diarrhea in children under five years of age who resided in rural environments but attended a hospital in Malindi, a coastal town in Kenya. Bacterial diarrhea was found in 239...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1997.tb01925.x
更新日期:1997-01-01 00:00:00
abstract::Adult T-cell leukemia-associated membrane antigen (ATLMA) expressed on the surface of living ATL virus (ATLV)-carrying cells was investigated by an indirect membrane immunofluorescence method using natural antibodies to ATLV in human sera. All the ATLV-positive cell lines tested that had cytoplasmic ATL-associated ant...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1984.tb00696.x
更新日期:1984-01-01 00:00:00
abstract::A cytotoxic T lymphocyte (CTL) response to dengue virus-infected target cells is described. Effector cells were generated in an in vitro secondary culture and appeared to be T cells possessing both the Lyt 1.1 and Lyt 2.1 surface antigens. A stronger CTL response was noted with the H-2k haplotype compared to H-2d, and...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1988.tb01411.x
更新日期:1988-01-01 00:00:00
abstract::Based on the results of a phylogenetic analysis of 16S rRNA and the presence of sphingoglycolipid in cellular lipids of the type strains, transfer of "Rhizomonas" suberifaciens, Blastomonas natatoria and Erythromonas ursincola to the genus Sphingomonas as Sphingomonas suberifaciens (van Bruggen et al 1990) comb. nov.,...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1999.tb02414.x
更新日期:1999-01-01 00:00:00
abstract::The standard products of V(D)J recombination of immunoglobulin and T cell receptor genes are two kinds of DNA junction, a coding joint and a signal joint. TCR delta V-D and D-D signal joints in adult mouse thymocytes were sequenced following PCR amplification. We observed differential nucleotide insertions at the V de...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.2003.tb03455.x
更新日期:2003-01-01 00:00:00
abstract::The ability of 33 compounds of L-alanine analogues over a wide range of concentrations to initiate germination of Bacillus subtilis spores was determined, and the inhibitory activity against L-alanine-initiated germination was determined for the above compounds and 22 of their D- and DL-isomers. Nineteen L-isomers wer...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1985.tb00822.x
更新日期:1985-01-01 00:00:00
abstract::The mitogen effect on migration of eosinophils and monocytes was studied in embryonic chickens. On the 13th embryonic day, chickens were injected with mitogens, such as concanavalin A (Con A), phytohemagglutinin-P (PHA-P), and lipopolysaccharide (LPS), into the allantoic cavity, and the mitogenic effect was estimated ...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1979.tb00491.x
更新日期:1979-01-01 00:00:00