Protective effects of in vivo-expressed autotransporters against Bordetella pertussis infection.

abstract：:Treatment of macrophages with cytochalasin E in combination with a lectin was found to stimulate the generation of superoxide anions (O2-) very efficiently. The macrophages stimulated with concanavalin A, phytohemagglutinin or wheat germ agglutinin released superoxide, but cells pretreated with cytochalasin E release...

journal_title：Microbiology and immunology

authors： Kayashima K,Onoue K,Nakagawara A,Minakami S

更新日期：1980-01-01 00:00:00

abstract：:Electron microscopy revealed multi-layered membranes within the cytoplasmic inclusion (accumulation of nucleocapsids) produced by rabies virus. When infected BHK cells were maintained at 31 C, an enhancement in production of these membranes occurred in approximately 60% of inclusion-containing cells. Multi-layered mem...

journal_title：Microbiology and immunology

authors： Tanabe K,Matsumoto S

更新日期：1979-01-01 00:00:00

abstract：:Methylcellulose-elicited peritoneal macrophages from BALB/c mice were cultivated in vitro and inoculated with dengue virus (DV). At intervals thereafter portions of the culture fluids were taken and titrated for viral infectivity. Extracts from Ascaris suum and Parascaris equorum, either crude or Sephadex G-100 fracti...

journal_title：Microbiology and immunology

authors： Wiharta AS,Hotta H,Hotta S,Matsumura T,Sujudi,Tsuji M

更新日期：1985-01-01 00:00:00

abstract：:The heat-labile toxin (HLT) of Bordetella bronchiseptica was purified successively from sonic extracts of phase I organisms grown in Stainer-Scholte medium, by partition in hydrophobic interaction, sucrose density gradient centrifugation, gel filtration through Sepharose 4B and 6B, isoelectric precipitation and isoele...

journal_title：Microbiology and immunology

authors： Endoh M,Amitani M,Nakase Y

更新日期：1986-01-01 00:00:00

abstract：:ICR mice were immunized with sheep red blood cells (sRBC). Both adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) activities in spleen lymphocytes increased faster than the serum antibody titer and reached a peak one week after the immunization. ADA activity increased significantly in T lymphocytes b...

journal_title：Microbiology and immunology

authors： Kurashige S,Akuzawa Y,Yoshida T,Teshima C,Kodama K,Mitsuhashi S

更新日期：1982-01-01 00:00:00

abstract：:Murine B cell mitogens such as bacterial lipopolysaccharide (LPS), butanol-extracted water soluble adjuvant (Bu-WSA), dextran sulfate (DS), synthetic muramyl dipeptide (MDP), and its analog MDP-Lys (L18) do not show any mitogenic ability in vitro on human peripheral blood lymphocytes or mixed cell populations of purif...

journal_title：Microbiology and immunology

authors： Nitta T,Konno-Ejiri H,Okumura S,Ozawa A,Nakano M

更新日期：1985-01-01 00:00:00

abstract：:A serological typing scheme of Bacillus cereus has been developed by immunochemical analyses of flagellar antigen using an agglutination method. Enzyme-linked immunosorbent assay (ELISA) for the classification of flagellar serotype of Bacillus cereus had greater sensitivity. 10-500 times, than that of agglutination me...

journal_title：Microbiology and immunology

authors： Murakami T,Hiraoka K,Mikami T,Matsumoto T,Katagiri S,Suzuki M

更新日期：1991-01-01 00:00:00

abstract：:Human adenoviruses (HAdVs) are an important cause of acute gastroenteritis in children. However, few studies on the epidemiology or types of HAdVs associated with acute gastroenteritis have been conducted in Korea. Therefore, in the present study, the incidence of HAdV in 2064 stool samples from Korean children hospit...

journal_title：Microbiology and immunology

authors： Lee JI,Lee GC,Chung JY,Han TH,Lee YK,Kim MS,Lee CH

更新日期：2012-08-01 00:00:00

abstract：:The biological effect of purified beta toxin of Clostridium perfringens type C in vivo was investigated. After intravenous injection of the purified beta toxin into rats, a rise in blood pressure and a simultaneous fall in heart rate were observed. After the blood pressure reached a maximum, the heart rate recovered g...

journal_title：Microbiology and immunology

authors： Sakurai J,Fujii Y,Matsuura M,Endo K

更新日期：1981-01-01 00:00:00

abstract：:We cloned and sequenced two new Verotoxin 2 (VT2) variant genes: one from an Escherichia coli strain from a case of bovine diarrhea and the other from an E. coli strain from a patient with diarrhea. The nucleotide and amino acid sequences of these two genes were highly homologous with, but distinct from those of the V...

journal_title：Microbiology and immunology

authors： Lin Z,Yamasaki S,Kurazono H,Ohmura M,Karasawa T,Inoue T,Sakamoto S,Suganami T,Takeoka T,Taniguchi Y

更新日期：1993-01-01 00:00:00

abstract：:Many studies have proved the relevance of local immune responses, rather than systemic immunity, to the pathogenesis of allergic rhinitis. Indeed, allergen-specific B lymphocyte undergoes class switching to IgE in situ. However, the relative contribution of in situ production to the amount in serum is still ambiguous....

journal_title：Microbiology and immunology

authors： Yoshida T,Usui A,Kusumi T,Inafuku S,Sugiyama T,Koide N,Yokochi T

更新日期：2005-01-01 00:00:00

abstract：:In recent years, phosphatidylinositol 4-kinase III beta (PI4KB) has emerged as a conserved target of anti-picornavirus compounds. In the present study, PI4KB was identified as the direct target of the plant-derived anti-picornavirus compounds, oxoglaucine and pachypodol (also known as Ro 09-0179). PI4KB was also ident...

journal_title：Microbiology and immunology

authors： Arita M,Philipov S,Galabov AS

更新日期：2015-06-01 00:00:00

abstract：:CD4+ cytotoxic T lymphocytes (CTL) clones, YT-4 and YT-9, specific for Toxoplasma gondii (T. gondii)-infected melanoma SK-MEL 28 (P36), were generated from the peripheral blood lymphocytes (PBL) of a patient with chronic toxoplasmosis. These CTL clones were shown to secrete significant amounts of interleukin 6 (IL-6) ...

journal_title：Microbiology and immunology

authors： Yang TH,Aosai F,Norose K,Ueda M,Yano A

更新日期：1996-01-01 00:00:00

abstract：:Shigella sonnei, which has generally been associated with dysentery in developed countries, has recently been emerging in developing countries. Specifically, in Brazil few published studies have that molecularly characterized this species. The aims of this study were to analyze the efficacy of typing using multiple-lo...

journal_title：Microbiology and immunology

authors： Seribelli AA,Frazão MR,Medeiros MIC,Stehling EG,Falcão JP

更新日期：2017-12-01 00:00:00

abstract：:Accurate and rapid detection of carbapenemases and identification of their types in Enterobacteriaceae are both still major challenges for clinical laboratories in attempting to prevent the intrusion and transmission of carbapenemase-producing Enterobacteriaceae. This study aimed to evaluate the performance of the MAS...

journal_title：Microbiology and immunology

authors： Ohsaki Y,Kubo R,Hobson J,Davies M,Osaka S,Hayashi W,Taniguchi Y,Koide S,Nagano Y,Nagano N

更新日期：2018-01-01 00:00:00

abstract：:The present study deals with whether lipopolysaccharide (LPS)-induced intra-uterine fetal death (IUFD) is related to LPS-susceptibility of either mother or fetus and how LPS or LPS-induced TNF causes IUFD. LPS-susceptible C3H/HeN or -hypo-susceptible C3H/HeJ pregnant mice and the mice mated reciprocally with these mic...

journal_title：Microbiology and immunology

authors： Kohmura Y,Kirikae T,Kirikae F,Nakano M,Sato I

更新日期：2000-01-01 00:00:00

abstract：:A gene upstream from fimA, the gene encoding fimbrilin, on the chromosome of Porphyromonas gingivalis was sequenced and shown to be the gene encoding an outer membrane protein in this organism based on homology and biochemical analyses. Therefore, the gene (formerly ORF5) was designated pgmA, the P. gingivalis outer m...

journal_title：Microbiology and immunology

authors： Hongo H,Osano E,Ozeki M,Onoe T,Watanabe K,Honda O,Tani H,Nakamura H,Yoshimura F

更新日期：1999-01-01 00:00:00

abstract：:Mg2+-availability in Staphylococcus aureus cells decreased significantly with increasing NaCl concentration in growth media. Cells grew in a NaCl-free, Chelex resin-treated complex medium only if the medium was supplemented with 50 microM MgCl2, while, growth was limited when the medium was further supplemented with 1...

journal_title：Microbiology and immunology

authors： Ochiai T

更新日期：2001-01-01 00:00:00

abstract：:The effects of recombinant human interleukin-2 (rIL-2) administered prophylactically on the death of CBA/J mice challenged with Klebsiella pneumoniae 27 intraperitoneally were examined. rIL-2 administered subcutaneously at 20 micrograms per mouse for 7 days enhanced survival after a lethal challenge. The injection of ...

journal_title：Microbiology and immunology

authors： Iizawa Y,Nakao M,Kondo M,Yamazaki T

更新日期：1990-01-01 00:00:00

abstract：:Aged C57BL/6 (B6) mice could reject allogeneic BALB/c RL male 1 tumor as efficiently as young B6 mice. However, in vitro analysis showed impaired generation of cytotoxic T cell response in aged B6 mice against allogeneic tumor. The reaction could be augmented by the addition of recombinant interleukin-2 (rIL-2). Enzym...

journal_title：Microbiology and immunology

authors： Win S,Uenaka A,Nakayama E

更新日期：2002-01-01 00:00:00

abstract：:JC polyomavirus (JCPyV) causes progressive multifocal leukoencephalopathy (PML), a fatal demyelinating disease of the central nervous system, in immunocompromised patients. Because no drugs have been approved for treating PML, many antiviral agents are currently being investigated for this purpose. The inhibitory effe...

journal_title：Microbiology and immunology

authors： Nukuzuma S,Nakamichi K,Kameoka M,Sugiura S,Nukuzuma C,Tasaki T,Takegami T

更新日期：2016-04-01 00:00:00

abstract：:CD4(+) T cells play critical roles in protection against the blood stage of malarial infection; however, their uncontrolled activation can be harmful to the host. In this study, in which rodent models of Plasmodium parasites were used, the expression of inhibitory receptors on activated CD4(+) T cells and their cytoki...

journal_title：Microbiology and immunology

authors： Doe HT,Kimura D,Miyakoda M,Kimura K,Akbari M,Yui K

更新日期：2016-02-01 00:00:00

abstract：:Attempts were made to study the pathogenicity of some strains of Bacteroides fragilis group in the rat intra-abdominal abscess model. Multiple intraabdominal abscesses were produced in 50 to 70% of animals when an inoculum containing 10(9) CFU/ml of any of the five species of Bacteroides fragilis group was injected. R...

journal_title：Microbiology and immunology

authors： Jotwani R,Gupta U,Watanabe K,Ueno K

更新日期：1992-01-01 00:00:00

abstract：:Rapid western blot (WB) procedure for an abnormal isoform of prion protein (PrP(Sc) ) detection in lymphoid tissues was established and has been applied to the surveillance of fallen stock. In this program, brain and palatal tonsil were examined by WB and three cases of sheep scrapie were detected. While one clinicall...

journal_title：Microbiology and immunology

authors： Shimada K,Hayashi HK,Ookubo Y,Iwamaru Y,Imamura M,Takata M,Schmerr MJ,Shinagawa M,Yokoyama T

更新日期：2005-01-01 00:00:00

abstract：:Manipulation of viral genomes is essential for studying viral gene function and utilizing viruses for therapy. Several techniques for viral genome engineering have been developed. Homologous recombination in virus-infected cells has traditionally been used to edit viral genomes; however, the frequency of the expected ...

journal_title：Microbiology and immunology

authors： Suenaga T,Kohyama M,Hirayasu K,Arase H

更新日期：2014-09-01 00:00:00

abstract：:M protein is an important virulence determinant in Streptococcus pyogenes, but the amounts of M protein in various strains of the species remain to be elucidated. To assess the amount of M protein in strains of each emm genotype, dot blot analysis was performed on 141 clinically isolated strains. Among the cell membra...

journal_title：Microbiology and immunology

authors： Matsumoto M,Suzuki M,Hirose K,Hiramatsu R,Minagawa H,Minami M,Tatsuno I,Okamoto A,Ohta M,Hasegawa T

更新日期：2011-06-01 00:00:00

abstract：:Exocellular proteases produced by Vibrio fluvialis, V. furnissii, V. metschnikovii and V. campbellii were characterized and compared to those of V. mimicus protease (VMP) and V. vulnificus protease (VVP). These proteases possessed both elastolytic and hemagglutinating abilities and were identified, except that of V. m...

journal_title：Microbiology and immunology

authors： Alam M,Miyoshi S,Shinoda S

更新日期：1995-01-01 00:00:00

abstract：:The immune responses of individuals exposed to Leishmania major were evaluated and compared with those of non-exposed volunteers. Forty-one patients with active lesion(s), 43 healed individuals, 15 vaccinees 1 month or 1 year post vaccination, and 15 non-exposed volunteers were studied. Leishmanin skin test (LST) resp...

journal_title：Microbiology and immunology

authors： Alimohammadian MH,Darabi H,Malekzadeh S,Mahmoodzadeh-Niknam H,Ajdary S,Khamesipour A,Bahonar A,Mofarrah A

更新日期：2007-01-01 00:00:00

abstract：:The test for susceptibility to kanamycin is useful for differentiating between the genera Rhodococcus and Nocardia. All rhodococci except R. equi, R. erythropolis, and R. aurantiacus are susceptible to kanamycin, whereas all nocardiae except N. otitidis-caviarum are resistant to kanamycin. Tests for susceptibility to ...

journal_title：Microbiology and immunology

authors： Tsukamura M

更新日期：1988-01-01 00:00:00

abstract：:The mechanism of MHC-restricted T and B cell interactions in antibody response was studied with IgM-inducible B hybridomas and antigen-specific helper T cell clones. B hybridomas were prepared by fusion between splenic B cells from (CBA/N (H-2k) X BALB/c (H-2d)) F1 (NBF1) male mice and a B lymphoma cell line, M12.4.5....

journal_title：Microbiology and immunology

authors： Yoshikubo T,Kimoto M,Kishimoto T

更新日期：1985-01-01 00:00:00